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93
Chem Impex International v5111 boc paf chem impex
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Promega junior lb 9059 luminometer
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Journal: Cell chemical biology

Article Title: Chemoselective restoration of para -azido-phenylalanine at multiple sites in proteins

doi: 10.1016/j.chembiol.2021.12.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli C321.ΔA ( Lajoie et al., 2013 ) RRID: Addgene_48998 Chemicals, peptides, and recombinant proteins para -azido-L-phenylalanine Bachem Cat# F-3075 para -amino-L-phenylalanine Sigma Aldrich Cat# 9193 Imidazole-1-sulfonyl azide Sigma Aldrich Cat# 902756 10X PBS (pH7.2) Gibco Cat# 70013032 Thermolysin Promega Cat# V4001 Trypsin Promega Cat# V5111 Boc-pAF Chem Impex Cat# 02720 Lys(Boc) Sigma Aldrich Cat# 359661 Boc-Lys Sigma Aldrich Cat# 359688 Cy5.5 alkyne Lumiprobe Cat# B70B0 Tris(3-hydroxypropyl-triazolylmethyl)amine Sigma Aldrich Cat# 762342 Copper sulfate pentahydrate Sigma Aldrich Cat# 209198 Aminoguanidine hydrochloride Sigma Aldrich Cat# 396494 Peptide 1 ChinaPeptides Co., Ltd Custom Peptide 2 ChinaPeptides Co., Ltd Custom Peptide 3 ChinaPeptides Co., Ltd Custom Peptide 4 Tufts University Core Facility Custom Peptide 5 Tufts University Core Facility Custom Bovine Serum Albumin Sigma Aldrich Cat# 05470 Human Ubiquitin R&D Systems Cat# U-100H GFP(pAzF) This paper N/A ELP(5pAzF)GFP This paper N/A ELP(10pAzF)GFP This paper N/A Recombinant DNA pEvol-pAcFRS.1.t1 ( Amiram et al., 2015 ) RRID: Addgene_73545 Software and algorithms Adobe Illustrator CS6 Adobe https://www.adobe.com/ BioRender BioRender https://www.BioRender.com ChemDraw PerkinElmer https://www.perkinelmer.com/category/chemdraw GraphPad Prism GraphPad https://www.graphpad.com/scientific-software/prism/ Marvin 17.2.27.0 ChemAxon https://chemaxon.com/products/marvin MASCOT Matrix Science https://www.matrixscience.com MassHunter Qualitative Analysis (Version B.07.00) Agilent Technologies https://www.agilent.com/en/product/software-informatics/mass-spectrometry-software MassHunter Workstation Data Acquistion (Version B.06.01) Agilent Technologies https://www.agilent.com/en/product/software-informatics/mass-spectrometry-software MaxQuant ( Cox and Mann, 2008 ) https://www.maxquant.org Unidec ( Reid et al., 2019 ) http://unidec.chem.ox.ac.uk XCalibur 4.2 Thermo Fisher Scientific https://www.thermofisher.com/order/catalog/product/OPTON-30965 Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Recombinant, Software