90094 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Bio-Techne corporation rabbit polyclonal septin4 antibody
    Formation of the basal body and annulus is deregulated in Dnah2 Δ/Δ epididymal sperm. (A,B) Confocal images of immunostained centriolar (A) and annulus (B) proteins in WT ( left ) and Dnah2 Δ/Δ ( right ) epididymal sperm. Centriolar proteins, CENTRIN1 ( top ) and γ-Tubulin ( bottom ), lacking in mouse epididymal sperm, are aberrantly detected near the connecting piece of Dnah2 Δ/Δ sperm (A) . Annulus components, <t>SEPTIN4</t> ( top ) and SEPTIN12 ( bottom ), is localized heterogeneously along the flagella in Dnah2 Δ/Δ sperm (B) . Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown (A,B) . Magnified insets are represented (scale bars = 1 μm). (C,D) Transmission electron microscopy of normal (C) and Dnah2 Δ/Δ (D) epididymal sperm. Dnah2 +/Δ ( top ) and WT (bottom) sperm were used for controls to show ultrastructure of connecting piece lacking centrioles and annulus, respectively (C) . Centriole-like structure or the traces are detected (red asterisks) and annulus-like electron-dense area (yellow asterisks) is localized heterogeneously near the connecting piece in Dnah2 Δ/Δ sperm (D) . Nu, nucleus; BP, basal plate; CP, capitulum; SC, segmented column; PCV, proximal centriolar vault; DCV, distal centriolar vault; AN, annulus; AX, axoneme; M, mitochondria; ODF, outer dense fiber; MP, midpiece; PP, principal piece.
    Rabbit Polyclonal Septin4 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal septin4 antibody/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal septin4 antibody - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    90
    BOC Sciences chemicals boc sciences
    Formation of the basal body and annulus is deregulated in Dnah2 Δ/Δ epididymal sperm. (A,B) Confocal images of immunostained centriolar (A) and annulus (B) proteins in WT ( left ) and Dnah2 Δ/Δ ( right ) epididymal sperm. Centriolar proteins, CENTRIN1 ( top ) and γ-Tubulin ( bottom ), lacking in mouse epididymal sperm, are aberrantly detected near the connecting piece of Dnah2 Δ/Δ sperm (A) . Annulus components, <t>SEPTIN4</t> ( top ) and SEPTIN12 ( bottom ), is localized heterogeneously along the flagella in Dnah2 Δ/Δ sperm (B) . Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown (A,B) . Magnified insets are represented (scale bars = 1 μm). (C,D) Transmission electron microscopy of normal (C) and Dnah2 Δ/Δ (D) epididymal sperm. Dnah2 +/Δ ( top ) and WT (bottom) sperm were used for controls to show ultrastructure of connecting piece lacking centrioles and annulus, respectively (C) . Centriole-like structure or the traces are detected (red asterisks) and annulus-like electron-dense area (yellow asterisks) is localized heterogeneously near the connecting piece in Dnah2 Δ/Δ sperm (D) . Nu, nucleus; BP, basal plate; CP, capitulum; SC, segmented column; PCV, proximal centriolar vault; DCV, distal centriolar vault; AN, annulus; AX, axoneme; M, mitochondria; ODF, outer dense fiber; MP, midpiece; PP, principal piece.
    Chemicals Boc Sciences, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemicals boc sciences/product/BOC Sciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemicals boc sciences - by Bioz Stars, 2024-05
    90/100 stars
      Buy from Supplier

    86
    Thermo Fisher part no 90094
    Formation of the basal body and annulus is deregulated in Dnah2 Δ/Δ epididymal sperm. (A,B) Confocal images of immunostained centriolar (A) and annulus (B) proteins in WT ( left ) and Dnah2 Δ/Δ ( right ) epididymal sperm. Centriolar proteins, CENTRIN1 ( top ) and γ-Tubulin ( bottom ), lacking in mouse epididymal sperm, are aberrantly detected near the connecting piece of Dnah2 Δ/Δ sperm (A) . Annulus components, <t>SEPTIN4</t> ( top ) and SEPTIN12 ( bottom ), is localized heterogeneously along the flagella in Dnah2 Δ/Δ sperm (B) . Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown (A,B) . Magnified insets are represented (scale bars = 1 μm). (C,D) Transmission electron microscopy of normal (C) and Dnah2 Δ/Δ (D) epididymal sperm. Dnah2 +/Δ ( top ) and WT (bottom) sperm were used for controls to show ultrastructure of connecting piece lacking centrioles and annulus, respectively (C) . Centriole-like structure or the traces are detected (red asterisks) and annulus-like electron-dense area (yellow asterisks) is localized heterogeneously near the connecting piece in Dnah2 Δ/Δ sperm (D) . Nu, nucleus; BP, basal plate; CP, capitulum; SC, segmented column; PCV, proximal centriolar vault; DCV, distal centriolar vault; AN, annulus; AX, axoneme; M, mitochondria; ODF, outer dense fiber; MP, midpiece; PP, principal piece.
    Part No 90094, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/part no 90094/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    part no 90094 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier


    Image Search Results


    Formation of the basal body and annulus is deregulated in Dnah2 Δ/Δ epididymal sperm. (A,B) Confocal images of immunostained centriolar (A) and annulus (B) proteins in WT ( left ) and Dnah2 Δ/Δ ( right ) epididymal sperm. Centriolar proteins, CENTRIN1 ( top ) and γ-Tubulin ( bottom ), lacking in mouse epididymal sperm, are aberrantly detected near the connecting piece of Dnah2 Δ/Δ sperm (A) . Annulus components, SEPTIN4 ( top ) and SEPTIN12 ( bottom ), is localized heterogeneously along the flagella in Dnah2 Δ/Δ sperm (B) . Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown (A,B) . Magnified insets are represented (scale bars = 1 μm). (C,D) Transmission electron microscopy of normal (C) and Dnah2 Δ/Δ (D) epididymal sperm. Dnah2 +/Δ ( top ) and WT (bottom) sperm were used for controls to show ultrastructure of connecting piece lacking centrioles and annulus, respectively (C) . Centriole-like structure or the traces are detected (red asterisks) and annulus-like electron-dense area (yellow asterisks) is localized heterogeneously near the connecting piece in Dnah2 Δ/Δ sperm (D) . Nu, nucleus; BP, basal plate; CP, capitulum; SC, segmented column; PCV, proximal centriolar vault; DCV, distal centriolar vault; AN, annulus; AX, axoneme; M, mitochondria; ODF, outer dense fiber; MP, midpiece; PP, principal piece.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice

    doi: 10.3389/fcell.2021.662903

    Figure Lengend Snippet: Formation of the basal body and annulus is deregulated in Dnah2 Δ/Δ epididymal sperm. (A,B) Confocal images of immunostained centriolar (A) and annulus (B) proteins in WT ( left ) and Dnah2 Δ/Δ ( right ) epididymal sperm. Centriolar proteins, CENTRIN1 ( top ) and γ-Tubulin ( bottom ), lacking in mouse epididymal sperm, are aberrantly detected near the connecting piece of Dnah2 Δ/Δ sperm (A) . Annulus components, SEPTIN4 ( top ) and SEPTIN12 ( bottom ), is localized heterogeneously along the flagella in Dnah2 Δ/Δ sperm (B) . Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown (A,B) . Magnified insets are represented (scale bars = 1 μm). (C,D) Transmission electron microscopy of normal (C) and Dnah2 Δ/Δ (D) epididymal sperm. Dnah2 +/Δ ( top ) and WT (bottom) sperm were used for controls to show ultrastructure of connecting piece lacking centrioles and annulus, respectively (C) . Centriole-like structure or the traces are detected (red asterisks) and annulus-like electron-dense area (yellow asterisks) is localized heterogeneously near the connecting piece in Dnah2 Δ/Δ sperm (D) . Nu, nucleus; BP, basal plate; CP, capitulum; SC, segmented column; PCV, proximal centriolar vault; DCV, distal centriolar vault; AN, annulus; AX, axoneme; M, mitochondria; ODF, outer dense fiber; MP, midpiece; PP, principal piece.

    Article Snippet: Rabbit polyclonal SEPTIN4 antibody (NBP1-90093) was purchased from Novus Biologicals.

    Techniques: Staining, Fluorescence, Transmission Assay, Electron Microscopy

    DNAH2 deficiency dysregulates the expression and localization of the basal body and annulus components in developing spermatids. (A,B) DNAH2 mRNA expression in human (A) and mouse (B) testicular cells. Human (GSE109037) and mouse (GSE109033) testis single-cell RNA (scRNA)-seq datasets were analyzed. UMAP plots represents 7 and 11 clusters of testicular cells in human and mouse ( left ) and DNAH2 mRNA expression in individual cells ( right ). EC, endothelial cell; PTM, peritubular myoid cell. (C,D) Confocal images of centriole (CENTRIN1, C ) and annulus (SEPTIN4, D ) proteins in WT and Dnah2 Δ/Δ spermatids in different developmental step (S). The number of centrioles and localization of the annulus are impaired heterogeneously in Dnah2 Δ/Δ spermatids. Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown. Magnified insets are shown with fluorescence images (scale bars = 1 mm, C,D ). (E) A schematic cartoon depicting impaired cellular events by DNAH2 deficiency during sperm development. Testicular germ cells from round to elongated spermatids and mature sperm are drawn ( top ) and the corresponding morphological and structural changes are described in a gray box ( bottom ). DNAH2 deficiency compromises flagella elongation and compartmentalization in developing spermatids, which causes in MMAF in mature sperm eventually.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice

    doi: 10.3389/fcell.2021.662903

    Figure Lengend Snippet: DNAH2 deficiency dysregulates the expression and localization of the basal body and annulus components in developing spermatids. (A,B) DNAH2 mRNA expression in human (A) and mouse (B) testicular cells. Human (GSE109037) and mouse (GSE109033) testis single-cell RNA (scRNA)-seq datasets were analyzed. UMAP plots represents 7 and 11 clusters of testicular cells in human and mouse ( left ) and DNAH2 mRNA expression in individual cells ( right ). EC, endothelial cell; PTM, peritubular myoid cell. (C,D) Confocal images of centriole (CENTRIN1, C ) and annulus (SEPTIN4, D ) proteins in WT and Dnah2 Δ/Δ spermatids in different developmental step (S). The number of centrioles and localization of the annulus are impaired heterogeneously in Dnah2 Δ/Δ spermatids. Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown. Magnified insets are shown with fluorescence images (scale bars = 1 mm, C,D ). (E) A schematic cartoon depicting impaired cellular events by DNAH2 deficiency during sperm development. Testicular germ cells from round to elongated spermatids and mature sperm are drawn ( top ) and the corresponding morphological and structural changes are described in a gray box ( bottom ). DNAH2 deficiency compromises flagella elongation and compartmentalization in developing spermatids, which causes in MMAF in mature sperm eventually.

    Article Snippet: Rabbit polyclonal SEPTIN4 antibody (NBP1-90093) was purchased from Novus Biologicals.

    Techniques: Expressing, Staining, Fluorescence