8-cineole Search Results


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  • 99
    ATCC 1 8 cineole
    1 8 Cineole, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore 1 8 cineole
    Effect of <t>1,8-cineole</t> on UVB-induced skin tumorigenesis in SKH-1 hairless mouse skin (a) The external appearance of mice at 22 weeks after UVB-treatment. The mice in control group (n = 5) were subjected to the topical treatment of 200 μL acetone on dorsal skin without UVB irradiation (0.20 J/cm 2 ) at 3 days/week for 22 weeks. The mice in UVB-irradiated groups (n = 5 per each group) were treated with 200 μL acetone or the indicated amount of 1,8-cineole (40 nmol or 200 nmol) in 200 μL acetone topically on the dorsal skin for 1 hour before UVB irradiation (0.20 J/cm 2 ) for 3 days/week for 22 weeks. (b) 1,8-cineole suppresses UVB-induced tumor incidence in SKH-1 hairless mice. The incidence of skin tumors was recorded weekly. A tumor was defined as an outgrowth > 1 mm in diameter that persisted for 2 weeks or longer. Tumor incidence and multiplicity were recorded each week until the end of the experiment at 22 weeks. Asterisk symbols ( * and ** ) indicates a significant difference (p
    1 8 Cineole, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 8 cineole/product/Millipore
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    91
    Tokyo Chemical Industry 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 8 cineole/product/Tokyo Chemical Industry
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    99
    FUJIFILM 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck KGaA 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher compounds 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    Compounds 1 8 Cineole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    ATCC 1 8 cineole biofilm inhibition effect against s aureus atcc 6538
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole Biofilm Inhibition Effect Against S Aureus Atcc 6538, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Greiner Bio 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck & Co 1 8 cineole
    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of <t>1,8-cineole</t> in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.
    1 8 Cineole, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 8 cineole/product/Merck & Co
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    Nacalai 1 8 cineole
    Effects of Eucalyptus oil (Eu) and <t>1,8-cineole</t> on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). ( a ) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P
    1 8 Cineole, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Sinopharm 1 8 cineole
    Effects of Eucalyptus oil (Eu) and <t>1,8-cineole</t> on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). ( a ) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P
    1 8 Cineole, supplied by Sinopharm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Vetter 1 8 cineole
    Effects of Eucalyptus oil (Eu) and <t>1,8-cineole</t> on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). ( a ) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P
    1 8 Cineole, supplied by Vetter, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore congo red 1 8 cineol
    Effects of Eucalyptus oil (Eu) and <t>1,8-cineole</t> on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). ( a ) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P
    Congo Red 1 8 Cineol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher eo constituent 1 8 cineole eucalyptol
    Schematic representation of immune-modulatory mode of action of eucalyptus oil and its constituent 1, 8-cineole on different targets of LPS/infection-induced pathways in alveolar macrophage. Abbreviations : EO, Eucalyptus oil; Cin, 1,8-Cineole; LPS, Lipopolysaccharide; TLR, Toll-like receptor; LBP, LPS-binding protein; CD14, Cluster of differentiation 14; TREM-1, Triggering receptor expressed on myeloid cells 1; IRAKs, IL-1 Receptor-Associated Kinases; TRAF6, TNF receptor-associated factor 6; MAPKs, Mitogen-activated protein kinases; ERK, Extracellular signal–regulated kinases; JNK: c-Jun N-terminal kinases; MKP-1, MAP kinase phosphatase-1; NF-κB, Nuclear factor kappa-light-chain-enhancer of activated B cells; NLRP3, Nod-like receptor family pyrin domain containing 3; NO, Nitric oxide: TF, Transcription factor.
    Eo Constituent 1 8 Cineole Eucalyptol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Standard format Plasmid sent in bacteria as agar stab
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    N/A
    This gene encodes a member of the cytochrome P450 superfamily of enzymes The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol
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    Standard format Plasmid sent in bacteria as agar stab
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    Image Search Results


    Effect of 1,8-cineole on UVB-induced skin tumorigenesis in SKH-1 hairless mouse skin (a) The external appearance of mice at 22 weeks after UVB-treatment. The mice in control group (n = 5) were subjected to the topical treatment of 200 μL acetone on dorsal skin without UVB irradiation (0.20 J/cm 2 ) at 3 days/week for 22 weeks. The mice in UVB-irradiated groups (n = 5 per each group) were treated with 200 μL acetone or the indicated amount of 1,8-cineole (40 nmol or 200 nmol) in 200 μL acetone topically on the dorsal skin for 1 hour before UVB irradiation (0.20 J/cm 2 ) for 3 days/week for 22 weeks. (b) 1,8-cineole suppresses UVB-induced tumor incidence in SKH-1 hairless mice. The incidence of skin tumors was recorded weekly. A tumor was defined as an outgrowth > 1 mm in diameter that persisted for 2 weeks or longer. Tumor incidence and multiplicity were recorded each week until the end of the experiment at 22 weeks. Asterisk symbols ( * and ** ) indicates a significant difference (p

    Journal: Oncotarget

    Article Title: 1,8-cineole prevents UVB-induced skin carcinogenesis by targeting the aryl hydrocarbon receptor

    doi: 10.18632/oncotarget.22519

    Figure Lengend Snippet: Effect of 1,8-cineole on UVB-induced skin tumorigenesis in SKH-1 hairless mouse skin (a) The external appearance of mice at 22 weeks after UVB-treatment. The mice in control group (n = 5) were subjected to the topical treatment of 200 μL acetone on dorsal skin without UVB irradiation (0.20 J/cm 2 ) at 3 days/week for 22 weeks. The mice in UVB-irradiated groups (n = 5 per each group) were treated with 200 μL acetone or the indicated amount of 1,8-cineole (40 nmol or 200 nmol) in 200 μL acetone topically on the dorsal skin for 1 hour before UVB irradiation (0.20 J/cm 2 ) for 3 days/week for 22 weeks. (b) 1,8-cineole suppresses UVB-induced tumor incidence in SKH-1 hairless mice. The incidence of skin tumors was recorded weekly. A tumor was defined as an outgrowth > 1 mm in diameter that persisted for 2 weeks or longer. Tumor incidence and multiplicity were recorded each week until the end of the experiment at 22 weeks. Asterisk symbols ( * and ** ) indicates a significant difference (p

    Article Snippet: Reagents and antibodies 1,8-cineole (99%) and N -Acetyl-L-cysteine (NAC, 99%) were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Mouse Assay, Irradiation

    Effect of 1,8-cineole on UVB-induced phosphorylation of MAPKs in HaCaT cells (a) and (b) 1,8-cineole inhibits UVB-induced phosphorylation of ERK1/2, MEK1/2, BRAF, and CRAF, (c) but not MKK4/7, JNK1/2, MKK3/6, and p38 in HaCaT cells. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then harvested after 15 min. Phosphorylation levels of proteins were detected by Western blotting.

    Journal: Oncotarget

    Article Title: 1,8-cineole prevents UVB-induced skin carcinogenesis by targeting the aryl hydrocarbon receptor

    doi: 10.18632/oncotarget.22519

    Figure Lengend Snippet: Effect of 1,8-cineole on UVB-induced phosphorylation of MAPKs in HaCaT cells (a) and (b) 1,8-cineole inhibits UVB-induced phosphorylation of ERK1/2, MEK1/2, BRAF, and CRAF, (c) but not MKK4/7, JNK1/2, MKK3/6, and p38 in HaCaT cells. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then harvested after 15 min. Phosphorylation levels of proteins were detected by Western blotting.

    Article Snippet: Reagents and antibodies 1,8-cineole (99%) and N -Acetyl-L-cysteine (NAC, 99%) were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Irradiation, Western Blot

    Effect of 1,8-cineole on UVB induction of the AhR/c-Src/EGFR signaling pathway and direct binding with AhR (a) 1,8-cineole inhibits UVB-induced phosphorylation of c-Src Tyr-416 and EGFR Tyr-845 residues in HaCaT cells. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then harvested after 15 min. Phosphorylation levels of proteins were detected by Western blotting. (b) and (c) 1,8-cineole suppresses UVB-induced AhR nuclear translocation and dissociation from c-Src in HaCaT cells. Cells were pretreated with 1,8-cineole for 1 hours, before irradiation with UVB for 30 min. (b) AhR (green) and p-c-Src (Tyr 416) (red) were detected by fluorescence microscopy. Nuclei were counterstained with DAPI (blue). White arrows indicate inhibition of AhR nuclear translocation and c-Src phosphorylation by 1,8-cineole treatment in HaCaT cells. Scale bar, 50 μm. (c) Co-immunoprecipitation assay was performed asdescribedin the ‘Materials and Methods’ and levels of AhR and c-Src were detected by Western blotting. (d) 1,8-cineole inhibits UVB-induced CYP1A1 mRNA upregulation in HaCaT cells. CYP1A1 mRNA levels were measured by qRT-PCR. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, and irradiated with UVB before total RNA was extracted after 4 hours. (e) 1,8-cineole directly binds to AhR but not (f) EGFR or (g) c-Src in HaCaT cells. For the DARTS assay, cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour and lysed. The lysates were digested with pronase (at the indicated pronase to protein ratio) and subjected to Western blotting.

    Journal: Oncotarget

    Article Title: 1,8-cineole prevents UVB-induced skin carcinogenesis by targeting the aryl hydrocarbon receptor

    doi: 10.18632/oncotarget.22519

    Figure Lengend Snippet: Effect of 1,8-cineole on UVB induction of the AhR/c-Src/EGFR signaling pathway and direct binding with AhR (a) 1,8-cineole inhibits UVB-induced phosphorylation of c-Src Tyr-416 and EGFR Tyr-845 residues in HaCaT cells. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then harvested after 15 min. Phosphorylation levels of proteins were detected by Western blotting. (b) and (c) 1,8-cineole suppresses UVB-induced AhR nuclear translocation and dissociation from c-Src in HaCaT cells. Cells were pretreated with 1,8-cineole for 1 hours, before irradiation with UVB for 30 min. (b) AhR (green) and p-c-Src (Tyr 416) (red) were detected by fluorescence microscopy. Nuclei were counterstained with DAPI (blue). White arrows indicate inhibition of AhR nuclear translocation and c-Src phosphorylation by 1,8-cineole treatment in HaCaT cells. Scale bar, 50 μm. (c) Co-immunoprecipitation assay was performed asdescribedin the ‘Materials and Methods’ and levels of AhR and c-Src were detected by Western blotting. (d) 1,8-cineole inhibits UVB-induced CYP1A1 mRNA upregulation in HaCaT cells. CYP1A1 mRNA levels were measured by qRT-PCR. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, and irradiated with UVB before total RNA was extracted after 4 hours. (e) 1,8-cineole directly binds to AhR but not (f) EGFR or (g) c-Src in HaCaT cells. For the DARTS assay, cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour and lysed. The lysates were digested with pronase (at the indicated pronase to protein ratio) and subjected to Western blotting.

    Article Snippet: Reagents and antibodies 1,8-cineole (99%) and N -Acetyl-L-cysteine (NAC, 99%) were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Binding Assay, Irradiation, Western Blot, Translocation Assay, Fluorescence, Microscopy, Inhibition, Co-Immunoprecipitation Assay, Quantitative RT-PCR

    Effect of 1,8-cineole on UVB-induced COX-2 expression and epidermal hyperplasia in SKH-1 hairless mouse skin (a) and (b) 1,8-cineole inhibits UVB-induced COX-2 expression in SKH-1 mouse skin. Expression levels of proteins were detected by Western blotting. Each band was densitometrically quantified by image analysis. The band density of COX-2 was normalized to β-actin followed by statistical analysis. Quantification data are represented as means ± SD (n = 3). Immunohistochemical analysis shows representative photographs of overall staining patterns from each group. The expression level of COX-2 proteins was stained as brown and the nucleus was counterstained as blue. Scale bar, 20 μm. (c) 1,8-cineole inhibis UVB-induced epidermal hyperplasia in SKH-1 mouse skin. Hematoxylin- and eosin-staining shows representative photographs of overall staining patterns from each group. Scale bar, 20 μm. Bars graph represent epidermal thickness (μm) of the indicated groups. Hash symbol ( # ) indicates a significant difference between the control and the UVB-treated group (p

    Journal: Oncotarget

    Article Title: 1,8-cineole prevents UVB-induced skin carcinogenesis by targeting the aryl hydrocarbon receptor

    doi: 10.18632/oncotarget.22519

    Figure Lengend Snippet: Effect of 1,8-cineole on UVB-induced COX-2 expression and epidermal hyperplasia in SKH-1 hairless mouse skin (a) and (b) 1,8-cineole inhibits UVB-induced COX-2 expression in SKH-1 mouse skin. Expression levels of proteins were detected by Western blotting. Each band was densitometrically quantified by image analysis. The band density of COX-2 was normalized to β-actin followed by statistical analysis. Quantification data are represented as means ± SD (n = 3). Immunohistochemical analysis shows representative photographs of overall staining patterns from each group. The expression level of COX-2 proteins was stained as brown and the nucleus was counterstained as blue. Scale bar, 20 μm. (c) 1,8-cineole inhibis UVB-induced epidermal hyperplasia in SKH-1 mouse skin. Hematoxylin- and eosin-staining shows representative photographs of overall staining patterns from each group. Scale bar, 20 μm. Bars graph represent epidermal thickness (μm) of the indicated groups. Hash symbol ( # ) indicates a significant difference between the control and the UVB-treated group (p

    Article Snippet: Reagents and antibodies 1,8-cineole (99%) and N -Acetyl-L-cysteine (NAC, 99%) were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, Western Blot, Immunohistochemistry, Staining

    Effect of 1,8-cineole on UVB-induced COX-2 protein, mRNA expression, and PGE2 generation in HaCaT cells (a) Chemical structure of 1,8-cineole. (b) 1,8-cineole inhibits UVB-induced COX-2 protein expression in HaCaT cells. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then harvested after 18 hours. Expression levels of COX-2 and β-actin were determined by Western blotting. Data are representative of three independent experiments that gave similar results. (c) 1,8-cineole inhibits UVB-induced COX-2 mRNA expression in HaCaT cells. COX-2 mRNA levels were measured by qRT-PCR. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then total RNA was extracted after 4 hours. Data are represented as the mean ± SD of three independent experiments. (d) 1,8-cineole inhibits UVB-induced PGE 2 generation in HaCaT cells. The quantity of PGE 2 in the culture medium was measured by ELISA. Data are represented as the mean ± SD of three independent experiments. Hash symbol ( # ) indicates a significant difference between the control and the UVB-treated group; asterisk symbols ( * and *** ) indicate significant differences (p

    Journal: Oncotarget

    Article Title: 1,8-cineole prevents UVB-induced skin carcinogenesis by targeting the aryl hydrocarbon receptor

    doi: 10.18632/oncotarget.22519

    Figure Lengend Snippet: Effect of 1,8-cineole on UVB-induced COX-2 protein, mRNA expression, and PGE2 generation in HaCaT cells (a) Chemical structure of 1,8-cineole. (b) 1,8-cineole inhibits UVB-induced COX-2 protein expression in HaCaT cells. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then harvested after 18 hours. Expression levels of COX-2 and β-actin were determined by Western blotting. Data are representative of three independent experiments that gave similar results. (c) 1,8-cineole inhibits UVB-induced COX-2 mRNA expression in HaCaT cells. COX-2 mRNA levels were measured by qRT-PCR. Cells were pre-treated with 1,8-cineole at the indicated concentrations for 1 hour, irradiated with UVB, and then total RNA was extracted after 4 hours. Data are represented as the mean ± SD of three independent experiments. (d) 1,8-cineole inhibits UVB-induced PGE 2 generation in HaCaT cells. The quantity of PGE 2 in the culture medium was measured by ELISA. Data are represented as the mean ± SD of three independent experiments. Hash symbol ( # ) indicates a significant difference between the control and the UVB-treated group; asterisk symbols ( * and *** ) indicate significant differences (p

    Article Snippet: Reagents and antibodies 1,8-cineole (99%) and N -Acetyl-L-cysteine (NAC, 99%) were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, Irradiation, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    ( a ) Macroscopic and microscopic views of liver of 20-week-old Pten KO mice without any treatment ( upper ; control) and 1,8-cineole treatment for eight weeks ( lower ; 1,8-cineole). The liver in the control group (first line upper) was enlarged, where as the liver in the 1,8-cineole group was smaller than that in the control group. HE stained livers of the control group (100× second line upper) and the 1,8-cineole group (100× second line lower) indicate vacuoles in hepatocytes were decreased in the 1,8-cineole group. Lipid accumulation was confirmed by Oil red O staining (100× control; third upper, 1,8-cineole group; third lower). Sirius red staining of the liver (400× control; fourth upper, 1,8-cineole; fourth lower). In the 1,8-cineole group, fibrotic area is narrower than in the control group; ( b ) Oil red O positive area (%) is compared between the two groups. *, p

    Journal: International Journal of Molecular Sciences

    Article Title: 1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation

    doi: 10.3390/ijms160612051

    Figure Lengend Snippet: ( a ) Macroscopic and microscopic views of liver of 20-week-old Pten KO mice without any treatment ( upper ; control) and 1,8-cineole treatment for eight weeks ( lower ; 1,8-cineole). The liver in the control group (first line upper) was enlarged, where as the liver in the 1,8-cineole group was smaller than that in the control group. HE stained livers of the control group (100× second line upper) and the 1,8-cineole group (100× second line lower) indicate vacuoles in hepatocytes were decreased in the 1,8-cineole group. Lipid accumulation was confirmed by Oil red O staining (100× control; third upper, 1,8-cineole group; third lower). Sirius red staining of the liver (400× control; fourth upper, 1,8-cineole; fourth lower). In the 1,8-cineole group, fibrotic area is narrower than in the control group; ( b ) Oil red O positive area (%) is compared between the two groups. *, p

    Article Snippet: Western Blot Analysis For western blot analysis, total protein extracts of control and 1,8-cineole group mice livers were obtained, then separated by 10% SDS-PAGE and transferred to nitrocellulose membrane (Millipore, Bedford, MA, USA).

    Techniques: Mouse Assay, Staining

    ( a ) Western blot of fatty acid synthase (FASN), phospho Akt (P-Akt), total Akt (T-Akt), phospho mTOR (P-mTOR), total mTOR (mTOR), Phospho-protein phosphatase type 2A (P-PP2A), total PP2A (PP2A), phospho-insulin receptor (P-insulin receptor), total insulin receptor (insulin receptor) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In the 1,8-cineole group, FASN, P-Akt, P-mTOR, and P-PP2A were decreased. P-insulin receptor was increased in the 1,8-cineole group. Densitometry of FASN/GAPDH ( b ), phospho/total Akt ( c ), phospho/total mTOR ( d ), phospho/total PP2A ( e ), and phospho/total insulin receptor ( f ) in the two groups ( n = 3). In the 1,8-cineole group, Akt and PP2A were significantly dephosphorylated, and the insulin receptor was significantly activated. * p

    Journal: International Journal of Molecular Sciences

    Article Title: 1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation

    doi: 10.3390/ijms160612051

    Figure Lengend Snippet: ( a ) Western blot of fatty acid synthase (FASN), phospho Akt (P-Akt), total Akt (T-Akt), phospho mTOR (P-mTOR), total mTOR (mTOR), Phospho-protein phosphatase type 2A (P-PP2A), total PP2A (PP2A), phospho-insulin receptor (P-insulin receptor), total insulin receptor (insulin receptor) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In the 1,8-cineole group, FASN, P-Akt, P-mTOR, and P-PP2A were decreased. P-insulin receptor was increased in the 1,8-cineole group. Densitometry of FASN/GAPDH ( b ), phospho/total Akt ( c ), phospho/total mTOR ( d ), phospho/total PP2A ( e ), and phospho/total insulin receptor ( f ) in the two groups ( n = 3). In the 1,8-cineole group, Akt and PP2A were significantly dephosphorylated, and the insulin receptor was significantly activated. * p

    Article Snippet: Western Blot Analysis For western blot analysis, total protein extracts of control and 1,8-cineole group mice livers were obtained, then separated by 10% SDS-PAGE and transferred to nitrocellulose membrane (Millipore, Bedford, MA, USA).

    Techniques: Western Blot

    Total ion chromatogram of rose hydrosol. 1 : 1,8-cineole (internal standard), 2 : linalool, 3 : 4-terpineol, 4 : alpha terpineol, 5 : citronellol, 6 : nerol, 7 : geraniol, 8 : 2-phenlyethanol, 9 : methyl eugenol, 10 : eugenol.

    Journal: Molecules

    Article Title: Effects of Orally Consumed Rosa damascena Mill. Hydrosol on Hematology, Clinical Chemistry, Lens Enzymatic Activity, and Lens Pathology in Streptozotocin-Induced Diabetic Rats

    doi: 10.3390/molecules24224069

    Figure Lengend Snippet: Total ion chromatogram of rose hydrosol. 1 : 1,8-cineole (internal standard), 2 : linalool, 3 : 4-terpineol, 4 : alpha terpineol, 5 : citronellol, 6 : nerol, 7 : geraniol, 8 : 2-phenlyethanol, 9 : methyl eugenol, 10 : eugenol.

    Article Snippet: Citronellol and 1,8-Cineole reference substances were purchased from Sigma–Aldrich (St. Louis, MO, USA).

    Techniques:

    Chemical structure of MMF (a), MPA (b), EUL (c), and NMP (d).

    Journal: Scientifica

    Article Title: Enhancement of Mycophenolate Mofetil Permeation for Topical Use by Eucalyptol and N-Methyl-2-pyrrolidone

    doi: 10.1155/2016/9672718

    Figure Lengend Snippet: Chemical structure of MMF (a), MPA (b), EUL (c), and NMP (d).

    Article Snippet: EUL (1,8 cineole), NMP, and triethylamine were purchased from Sigma-Aldrich (Denmark).

    Techniques:

    Skin permeation profiles of MMF (a) and MPA (b) at various concentration ratios of mixed enhancers between EUL and NMP incorporated to 300 μ g/mL of MMF preparation.

    Journal: Scientifica

    Article Title: Enhancement of Mycophenolate Mofetil Permeation for Topical Use by Eucalyptol and N-Methyl-2-pyrrolidone

    doi: 10.1155/2016/9672718

    Figure Lengend Snippet: Skin permeation profiles of MMF (a) and MPA (b) at various concentration ratios of mixed enhancers between EUL and NMP incorporated to 300 μ g/mL of MMF preparation.

    Article Snippet: EUL (1,8 cineole), NMP, and triethylamine were purchased from Sigma-Aldrich (Denmark).

    Techniques: Concentration Assay

    A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of 1,8-cineole in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.

    Journal: Journal of Fungi

    Article Title: A Solvent-Free Approach for Converting Cellulose Waste into Volatile Organic Compounds with Endophytic Fungi

    doi: 10.3390/jof4030102

    Figure Lengend Snippet: A comparison of the solid-phase extraction versus the liquid–liquid extraction (ethyl acetate/water). The growth media contained the degraded cellulose as a carbon source. A notable difference is the presence of 1,8-cineole in the liquid–liquid extraction. A comparison of the potato dextrose broth gave very similar results and, therefore, is not shown. The identity of 1,8-cineole was verified by comparison to an authentic standard.

    Article Snippet: A sample of 1,8-cineole was obtained from TCI chemicals (Portland, OR, USA).

    Techniques:

    Effects of Eucalyptus oil (Eu) and 1,8-cineole on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). ( a ) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P

    Journal: Scientific Reports

    Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling

    doi: 10.1038/s41598-020-77039-5

    Figure Lengend Snippet: Effects of Eucalyptus oil (Eu) and 1,8-cineole on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). ( a ) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P

    Article Snippet: 1,8-cineole was obtained from Nacalai Tesque (Kyoto, Japan), and dimethyl sulfoxide (DMSO) was acquired from Wako (Osaka, Japan).

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay

    Effect of 1,8-cineole on the phosphorylation of PLCγ, p38 and phospholipase A2 (PLA2). ( a – c ) 1,8-cineole was applied to bone-marrow-derived mast cells (BMMCs) after sensitisation with anti-DNP-IgE for 24 h, and cells were subsequently stimulated with DNP-human serum albumin (HSA). After 10 min, the cells were lysed, and SDS-PAGE was performed. Proteins were separated, and p-PLCγ, PLCγ, p-p38, p38, p-PLA2 and PLA2 expression was examined via immunoblotting. The left side presents representative blots for each protein, and the right bar graphs show the relative ratios of PLCγ, p38 and PLA2 phosphorylation. Phosphorylation ratios were calculated relative to PLCγ, p38 and PLA2 expression (each set as 1), and data are presented as the mean ± SEM of 4–6 independent experiments. Dunnett’s test was used to assess the statistical significance (** P

    Journal: Scientific Reports

    Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling

    doi: 10.1038/s41598-020-77039-5

    Figure Lengend Snippet: Effect of 1,8-cineole on the phosphorylation of PLCγ, p38 and phospholipase A2 (PLA2). ( a – c ) 1,8-cineole was applied to bone-marrow-derived mast cells (BMMCs) after sensitisation with anti-DNP-IgE for 24 h, and cells were subsequently stimulated with DNP-human serum albumin (HSA). After 10 min, the cells were lysed, and SDS-PAGE was performed. Proteins were separated, and p-PLCγ, PLCγ, p-p38, p38, p-PLA2 and PLA2 expression was examined via immunoblotting. The left side presents representative blots for each protein, and the right bar graphs show the relative ratios of PLCγ, p38 and PLA2 phosphorylation. Phosphorylation ratios were calculated relative to PLCγ, p38 and PLA2 expression (each set as 1), and data are presented as the mean ± SEM of 4–6 independent experiments. Dunnett’s test was used to assess the statistical significance (** P

    Article Snippet: 1,8-cineole was obtained from Nacalai Tesque (Kyoto, Japan), and dimethyl sulfoxide (DMSO) was acquired from Wako (Osaka, Japan).

    Techniques: Derivative Assay, SDS Page, Expressing

    Eucalyptus and 1,8-cineole are putative sites of inducible inhibition of mast cell degranulation.

    Journal: Scientific Reports

    Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling

    doi: 10.1038/s41598-020-77039-5

    Figure Lengend Snippet: Eucalyptus and 1,8-cineole are putative sites of inducible inhibition of mast cell degranulation.

    Article Snippet: 1,8-cineole was obtained from Nacalai Tesque (Kyoto, Japan), and dimethyl sulfoxide (DMSO) was acquired from Wako (Osaka, Japan).

    Techniques: Inhibition

    Effect of Eucalyptus oil (Eu) and 1,8-cineole on IgE-induced degranulation in bone-marrow-derived mast cells (BMMCs). ( a ) Effect of Eucalyptus oil and 1,8-cineole on BMMCs viability. Cells were treated with various concentrations of Eucalyptus oil or 1,8-cineole for 24 h. Next, cell viability was measured using the MTT assay. Relative cell viability was calculated by comparing the absorbance in cells treated with Eucalyptus oil or 1,8-cineole with that in cells treated with vehicle (0.1% DMSO). Data are expressed as the mean ± SEM (N = 3). ( b ) Eucalyptus oil or 1,8-cineole was applied to anti-DNP-IgE-sensitised BMMCs immediately after stimulation with DNP-human serum albumin (HSA), and after 1 h, β-hexosaminidase release was determined by comparing the enzymatic activity in BMMCs between treatment with vehicle (0.1% DMSO) and DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P

    Journal: Scientific Reports

    Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling

    doi: 10.1038/s41598-020-77039-5

    Figure Lengend Snippet: Effect of Eucalyptus oil (Eu) and 1,8-cineole on IgE-induced degranulation in bone-marrow-derived mast cells (BMMCs). ( a ) Effect of Eucalyptus oil and 1,8-cineole on BMMCs viability. Cells were treated with various concentrations of Eucalyptus oil or 1,8-cineole for 24 h. Next, cell viability was measured using the MTT assay. Relative cell viability was calculated by comparing the absorbance in cells treated with Eucalyptus oil or 1,8-cineole with that in cells treated with vehicle (0.1% DMSO). Data are expressed as the mean ± SEM (N = 3). ( b ) Eucalyptus oil or 1,8-cineole was applied to anti-DNP-IgE-sensitised BMMCs immediately after stimulation with DNP-human serum albumin (HSA), and after 1 h, β-hexosaminidase release was determined by comparing the enzymatic activity in BMMCs between treatment with vehicle (0.1% DMSO) and DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (** P

    Article Snippet: 1,8-cineole was obtained from Nacalai Tesque (Kyoto, Japan), and dimethyl sulfoxide (DMSO) was acquired from Wako (Osaka, Japan).

    Techniques: Derivative Assay, MTT Assay, Activity Assay

    1,8-cineole suppresses the elevation of intracellular Ca 2+ concentration. BMMCs sensitised with anti-DNP IgE were incubated in medium containing Fluo 4-AM. BMMCs were treated with various concentrations of 1,8-cineole and stimulated with DNP-HSA 60 s after initiating the monitoring of fluorescence intensity. ( a ) Time course of intracellular Ca 2 + concentration in BMMCs. Data are displayed as mean ± SEM (N = 5). ( b ) Relative fluorescence intensity 60 s after DNP-HSA stimulation. Data are displayed as mean ± SEM (N = 5). Statistical significance was assessed using Dunnett’s test (** P

    Journal: Scientific Reports

    Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling

    doi: 10.1038/s41598-020-77039-5

    Figure Lengend Snippet: 1,8-cineole suppresses the elevation of intracellular Ca 2+ concentration. BMMCs sensitised with anti-DNP IgE were incubated in medium containing Fluo 4-AM. BMMCs were treated with various concentrations of 1,8-cineole and stimulated with DNP-HSA 60 s after initiating the monitoring of fluorescence intensity. ( a ) Time course of intracellular Ca 2 + concentration in BMMCs. Data are displayed as mean ± SEM (N = 5). ( b ) Relative fluorescence intensity 60 s after DNP-HSA stimulation. Data are displayed as mean ± SEM (N = 5). Statistical significance was assessed using Dunnett’s test (** P

    Article Snippet: 1,8-cineole was obtained from Nacalai Tesque (Kyoto, Japan), and dimethyl sulfoxide (DMSO) was acquired from Wako (Osaka, Japan).

    Techniques: Concentration Assay, Incubation, Fluorescence

    Effect of 1,8-cineole on the phosphorylation of Syk and Lyn. ( a , b ) Eucalyptus oil or 1,8-cineole was applied to BMMCs sensitised with anti-DNP-IgE 24 h before stimulation with DNP-human serum albumin, and then after 10 min, cells were lysed, separated by SDS-PAGE and immunoblotted to analyse p-Syk, Syk, p-Lyn and Lyn expression. The left side presents representative examples of blots for each protein, and the right bar graphs present the phosphorylation relative ratios for Syk and Lyn.Phosphorylation ratios were calculated relative to Syk and Lyn expression (each set as 1), and data are presented as the mean ± SEM of 4–6 independent experiments. Dunnett’s test was used to assess the statistical significance (** P

    Journal: Scientific Reports

    Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling

    doi: 10.1038/s41598-020-77039-5

    Figure Lengend Snippet: Effect of 1,8-cineole on the phosphorylation of Syk and Lyn. ( a , b ) Eucalyptus oil or 1,8-cineole was applied to BMMCs sensitised with anti-DNP-IgE 24 h before stimulation with DNP-human serum albumin, and then after 10 min, cells were lysed, separated by SDS-PAGE and immunoblotted to analyse p-Syk, Syk, p-Lyn and Lyn expression. The left side presents representative examples of blots for each protein, and the right bar graphs present the phosphorylation relative ratios for Syk and Lyn.Phosphorylation ratios were calculated relative to Syk and Lyn expression (each set as 1), and data are presented as the mean ± SEM of 4–6 independent experiments. Dunnett’s test was used to assess the statistical significance (** P

    Article Snippet: 1,8-cineole was obtained from Nacalai Tesque (Kyoto, Japan), and dimethyl sulfoxide (DMSO) was acquired from Wako (Osaka, Japan).

    Techniques: SDS Page, Expressing

    Schematic representation of immune-modulatory mode of action of eucalyptus oil and its constituent 1, 8-cineole on different targets of LPS/infection-induced pathways in alveolar macrophage. Abbreviations : EO, Eucalyptus oil; Cin, 1,8-Cineole; LPS, Lipopolysaccharide; TLR, Toll-like receptor; LBP, LPS-binding protein; CD14, Cluster of differentiation 14; TREM-1, Triggering receptor expressed on myeloid cells 1; IRAKs, IL-1 Receptor-Associated Kinases; TRAF6, TNF receptor-associated factor 6; MAPKs, Mitogen-activated protein kinases; ERK, Extracellular signal–regulated kinases; JNK: c-Jun N-terminal kinases; MKP-1, MAP kinase phosphatase-1; NF-κB, Nuclear factor kappa-light-chain-enhancer of activated B cells; NLRP3, Nod-like receptor family pyrin domain containing 3; NO, Nitric oxide: TF, Transcription factor.

    Journal: PLoS ONE

    Article Title: Suppression of inflammatory and infection responses in lung macrophages by eucalyptus oil and its constituent 1,8-cineole: Role of pattern recognition receptors TREM-1 and NLRP3, the MAP kinase regulator MKP-1, and NFκB

    doi: 10.1371/journal.pone.0188232

    Figure Lengend Snippet: Schematic representation of immune-modulatory mode of action of eucalyptus oil and its constituent 1, 8-cineole on different targets of LPS/infection-induced pathways in alveolar macrophage. Abbreviations : EO, Eucalyptus oil; Cin, 1,8-Cineole; LPS, Lipopolysaccharide; TLR, Toll-like receptor; LBP, LPS-binding protein; CD14, Cluster of differentiation 14; TREM-1, Triggering receptor expressed on myeloid cells 1; IRAKs, IL-1 Receptor-Associated Kinases; TRAF6, TNF receptor-associated factor 6; MAPKs, Mitogen-activated protein kinases; ERK, Extracellular signal–regulated kinases; JNK: c-Jun N-terminal kinases; MKP-1, MAP kinase phosphatase-1; NF-κB, Nuclear factor kappa-light-chain-enhancer of activated B cells; NLRP3, Nod-like receptor family pyrin domain containing 3; NO, Nitric oxide: TF, Transcription factor.

    Article Snippet: Purified EO constituent 1,8-cineole (eucalyptol) was obtained commercially (Alfa Aesar, Reston, VA, USA); the working stock (2% vol/vol or 0.119 M) was prepared in 10% methanol (MeOH).

    Techniques: Infection, Binding Assay