8 oxo dgtp Search Results


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  • 86
    TriLink 8 oxo dgtp
    8 Oxo Dgtp, supplied by TriLink, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 oxo dgtp/product/TriLink
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8 oxo dgtp - by Bioz Stars, 2021-09
    86/100 stars
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    86
    TriLink 8 oxodgtp
    Kinetic analysis of dGTP and <t>8-oxodGTP</t> incorporation by KF − . Enzyme model used for (A) dGTP and (B) 8-oxodGTP. (C) Global fitting of incorporation of dGTP opposite of template dC. The concentrations of dGTP are 0.5 µM (closed circles),
    8 Oxodgtp, supplied by TriLink, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 oxodgtp/product/TriLink
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8 oxodgtp - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    94
    Jena Bioscience 8 oxo dgtp
    Kinetic analysis of dGTP and <t>8-oxodGTP</t> incorporation by KF − . Enzyme model used for (A) dGTP and (B) 8-oxodGTP. (C) Global fitting of incorporation of dGTP opposite of template dC. The concentrations of dGTP are 0.5 µM (closed circles),
    8 Oxo Dgtp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 oxo dgtp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8 oxo dgtp - by Bioz Stars, 2021-09
    94/100 stars
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    93
    GE Healthcare 8 oxo dgtp
    Standing start extension of primers having <t>8-oxo-G</t> at the termini by pol III* with or without β clamp. Cy3-labelled 35-mer primer (X = T, G or 8-oxo-G) with 100-mer template DNA (N = A or C) having biotin/streptavidin at both ends (20 nM) was incubated with pol III* (10 nM) and β clamp (0, 10 or 100 nM) in the presence of 100 μM dNTPs. The reaction mixtures were incubated at room temperature for 1 min. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. The alphabets shown in the figure represent: X, primer terminal base; N, template base; A, adenine; C, cytosine; G, guanine; T, thymine; oG, 8-oxo-G. The arrow indicates the position of primer. Although we have purified the primer DNA, there appears shorter primer DNAs, which were present below the position of the primer.
    8 Oxo Dgtp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 oxo dgtp/product/GE Healthcare
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8 oxo dgtp - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Kinetic analysis of dGTP and 8-oxodGTP incorporation by KF − . Enzyme model used for (A) dGTP and (B) 8-oxodGTP. (C) Global fitting of incorporation of dGTP opposite of template dC. The concentrations of dGTP are 0.5 µM (closed circles),

    Journal: Chemical research in toxicology

    Article Title: Klenow Fragment Discriminates Against the Incorporation of the Hyperoxidized dGTP Lesion Spiroiminodihydantoin into DNA

    doi: 10.1021/acs.chemrestox.5b00330

    Figure Lengend Snippet: Kinetic analysis of dGTP and 8-oxodGTP incorporation by KF − . Enzyme model used for (A) dGTP and (B) 8-oxodGTP. (C) Global fitting of incorporation of dGTP opposite of template dC. The concentrations of dGTP are 0.5 µM (closed circles),

    Article Snippet: To evaluate the biological relevance of incorporation of dSpTP into DNA from the nucleotide pool, we performed experiments to compare the kinetic parameters k pol and K d of the hyperoxidized nucleotide to dGTP and 8-oxodGTP.

    Techniques:

    (A) Sequence of primer and template strands. Primer was 32 P-radiolabeled at 5'-end as indicated by the asterisk. (B) Primer extension on different template DNA (X = A, C, G or T) with either dGTP, 8-oxodGTP, (S) -dSpTP, or (R) -dSpTP. The five lanes on

    Journal: Chemical research in toxicology

    Article Title: Klenow Fragment Discriminates Against the Incorporation of the Hyperoxidized dGTP Lesion Spiroiminodihydantoin into DNA

    doi: 10.1021/acs.chemrestox.5b00330

    Figure Lengend Snippet: (A) Sequence of primer and template strands. Primer was 32 P-radiolabeled at 5'-end as indicated by the asterisk. (B) Primer extension on different template DNA (X = A, C, G or T) with either dGTP, 8-oxodGTP, (S) -dSpTP, or (R) -dSpTP. The five lanes on

    Article Snippet: To evaluate the biological relevance of incorporation of dSpTP into DNA from the nucleotide pool, we performed experiments to compare the kinetic parameters k pol and K d of the hyperoxidized nucleotide to dGTP and 8-oxodGTP.

    Techniques: Sequencing

    Analysis of dGTP (open triangles), 8-oxodGTP (closed circles), (S) -dSpTP (open squares), and (R) -dSpTP (closed triangles) kinetic results using Michaelis-Menten techniques where the observed rates are plotted against nucleotide concentrations. The insert

    Journal: Chemical research in toxicology

    Article Title: Klenow Fragment Discriminates Against the Incorporation of the Hyperoxidized dGTP Lesion Spiroiminodihydantoin into DNA

    doi: 10.1021/acs.chemrestox.5b00330

    Figure Lengend Snippet: Analysis of dGTP (open triangles), 8-oxodGTP (closed circles), (S) -dSpTP (open squares), and (R) -dSpTP (closed triangles) kinetic results using Michaelis-Menten techniques where the observed rates are plotted against nucleotide concentrations. The insert

    Article Snippet: To evaluate the biological relevance of incorporation of dSpTP into DNA from the nucleotide pool, we performed experiments to compare the kinetic parameters k pol and K d of the hyperoxidized nucleotide to dGTP and 8-oxodGTP.

    Techniques:

    Single-Turnover Kinetic Analysis of dGTP and 8-oxodGTP Incorporation

    Journal: Chemical research in toxicology

    Article Title: Klenow Fragment Discriminates Against the Incorporation of the Hyperoxidized dGTP Lesion Spiroiminodihydantoin into DNA

    doi: 10.1021/acs.chemrestox.5b00330

    Figure Lengend Snippet: Single-Turnover Kinetic Analysis of dGTP and 8-oxodGTP Incorporation

    Article Snippet: To evaluate the biological relevance of incorporation of dSpTP into DNA from the nucleotide pool, we performed experiments to compare the kinetic parameters k pol and K d of the hyperoxidized nucleotide to dGTP and 8-oxodGTP.

    Techniques:

    Standing start extension of primers having 8-oxo-G at the termini by pol III* with or without β clamp. Cy3-labelled 35-mer primer (X = T, G or 8-oxo-G) with 100-mer template DNA (N = A or C) having biotin/streptavidin at both ends (20 nM) was incubated with pol III* (10 nM) and β clamp (0, 10 or 100 nM) in the presence of 100 μM dNTPs. The reaction mixtures were incubated at room temperature for 1 min. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. The alphabets shown in the figure represent: X, primer terminal base; N, template base; A, adenine; C, cytosine; G, guanine; T, thymine; oG, 8-oxo-G. The arrow indicates the position of primer. Although we have purified the primer DNA, there appears shorter primer DNAs, which were present below the position of the primer.

    Journal: Molecular Microbiology

    Article Title: Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains

    doi: 10.1111/mmi.12061

    Figure Lengend Snippet: Standing start extension of primers having 8-oxo-G at the termini by pol III* with or without β clamp. Cy3-labelled 35-mer primer (X = T, G or 8-oxo-G) with 100-mer template DNA (N = A or C) having biotin/streptavidin at both ends (20 nM) was incubated with pol III* (10 nM) and β clamp (0, 10 or 100 nM) in the presence of 100 μM dNTPs. The reaction mixtures were incubated at room temperature for 1 min. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. The alphabets shown in the figure represent: X, primer terminal base; N, template base; A, adenine; C, cytosine; G, guanine; T, thymine; oG, 8-oxo-G. The arrow indicates the position of primer. Although we have purified the primer DNA, there appears shorter primer DNAs, which were present below the position of the primer.

    Article Snippet: Deoxyribonucleoside triphosphates (ultrapure grade) and 8-oxo-dGTP were purchased from Amersham Pharmacia Biotech (Buckinghamshire, UK) and TriLink BioTechnologies (San Diego, CA, USA) respectively.

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis, Purification

    MutT mutator phenotype: a multi-polymerase affair. Pol III HE (oval) incorporates 8-oxo-dGTP (G*) opposite template A during the chromosomal replication. We suggest that while pol III accounts for nearly all 8-oxo-dGTP incorporation opposite template A, it only extends ≍ 30% of them, the remaining 70% being extended by the combined action of pol I, pol II, pol IV or pol V (based on data from Fig. 1 ). We also speculate that the roles of the auxiliary pols might be redundant because the mutation frequency was significantly reduced only when three or four auxiliary pols are deleted ( Fig. 1 ). Following a short patch (a thick line) of DNA synthesis by the auxiliary pols, pol III HE will resume chromosomal replication.

    Journal: Molecular Microbiology

    Article Title: Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains

    doi: 10.1111/mmi.12061

    Figure Lengend Snippet: MutT mutator phenotype: a multi-polymerase affair. Pol III HE (oval) incorporates 8-oxo-dGTP (G*) opposite template A during the chromosomal replication. We suggest that while pol III accounts for nearly all 8-oxo-dGTP incorporation opposite template A, it only extends ≍ 30% of them, the remaining 70% being extended by the combined action of pol I, pol II, pol IV or pol V (based on data from Fig. 1 ). We also speculate that the roles of the auxiliary pols might be redundant because the mutation frequency was significantly reduced only when three or four auxiliary pols are deleted ( Fig. 1 ). Following a short patch (a thick line) of DNA synthesis by the auxiliary pols, pol III HE will resume chromosomal replication.

    Article Snippet: Deoxyribonucleoside triphosphates (ultrapure grade) and 8-oxo-dGTP were purchased from Amersham Pharmacia Biotech (Buckinghamshire, UK) and TriLink BioTechnologies (San Diego, CA, USA) respectively.

    Techniques: Mutagenesis, DNA Synthesis

    Incorporation of 8-oxo-dGTP and extension by pol III* with or without β clamp under running start conditions. The 30-mer primer/100-mer streptavidin bound template (sequences 2, 20 nM) were incubated with pol III* (10 nM) with or without β clamp (100 nM) in the presence of indicated dNTPs (100 μM each) for 1 min at 25°C. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. The alphabets shown in the figure represent: A, dATP; C, dCTP; G, dGTP; T, dTTP; oG, 8-oxo-dGTP.

    Journal: Molecular Microbiology

    Article Title: Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains

    doi: 10.1111/mmi.12061

    Figure Lengend Snippet: Incorporation of 8-oxo-dGTP and extension by pol III* with or without β clamp under running start conditions. The 30-mer primer/100-mer streptavidin bound template (sequences 2, 20 nM) were incubated with pol III* (10 nM) with or without β clamp (100 nM) in the presence of indicated dNTPs (100 μM each) for 1 min at 25°C. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. The alphabets shown in the figure represent: A, dATP; C, dCTP; G, dGTP; T, dTTP; oG, 8-oxo-dGTP.

    Article Snippet: Deoxyribonucleoside triphosphates (ultrapure grade) and 8-oxo-dGTP were purchased from Amersham Pharmacia Biotech (Buckinghamshire, UK) and TriLink BioTechnologies (San Diego, CA, USA) respectively.

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis

    A. Exonuclease digestion of primers by pol III*. The Cy3-labelled 19-mer primer having G or 8-oxo-G at the 3′-termini/36-mer template having C or A at the position N (0.1 μM) were incubated with pol III* (1 nM) for 5, 10, 20 or 30 min at 25°C. The products were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. oG represents 8-oxo-G. B. Time course of digestion of primers by pol III*. Four types of primer/template DNA having G/C, G/A, 8-oxo-G/C or 8-oxo-G/A at the termini were incubated with pol III* (1 nM) for 5, 10, 20 or 30 min at 25°C and the percentage of the digested primer DNA was plotted. C. Excision of 8-oxo-dGMP at the end of the primer by pol III*, T7 pol, pol I (KF) and exo III. The 19-mer primer/36-mer template DNA (0.1 μM) or the 19-mer primer DNA alone (0.1 μM) was incubated with pol III* (1 nM) at room temperature, T7 pol (0.0001 unit μl −1 ), pol I (KF)(0.001 unit μl −1 ) or exo III (0.0001 unit μl −1 ) at 37°C for 10 min without dNTP. The products were analysed as described in the legend to A.

    Journal: Molecular Microbiology

    Article Title: Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains

    doi: 10.1111/mmi.12061

    Figure Lengend Snippet: A. Exonuclease digestion of primers by pol III*. The Cy3-labelled 19-mer primer having G or 8-oxo-G at the 3′-termini/36-mer template having C or A at the position N (0.1 μM) were incubated with pol III* (1 nM) for 5, 10, 20 or 30 min at 25°C. The products were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager. oG represents 8-oxo-G. B. Time course of digestion of primers by pol III*. Four types of primer/template DNA having G/C, G/A, 8-oxo-G/C or 8-oxo-G/A at the termini were incubated with pol III* (1 nM) for 5, 10, 20 or 30 min at 25°C and the percentage of the digested primer DNA was plotted. C. Excision of 8-oxo-dGMP at the end of the primer by pol III*, T7 pol, pol I (KF) and exo III. The 19-mer primer/36-mer template DNA (0.1 μM) or the 19-mer primer DNA alone (0.1 μM) was incubated with pol III* (1 nM) at room temperature, T7 pol (0.0001 unit μl −1 ), pol I (KF)(0.001 unit μl −1 ) or exo III (0.0001 unit μl −1 ) at 37°C for 10 min without dNTP. The products were analysed as described in the legend to A.

    Article Snippet: Deoxyribonucleoside triphosphates (ultrapure grade) and 8-oxo-dGTP were purchased from Amersham Pharmacia Biotech (Buckinghamshire, UK) and TriLink BioTechnologies (San Diego, CA, USA) respectively.

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis

    Incorporation of 8-oxo-dGTP by pol III*. The Cy3-labelled 18-mer primer/36-mer template (sequences 1, 0.1 μM) was treated with pol III* (1 nM) in the presence of 100 μM 8-oxo-dGTP. The reaction mixtures were incubated at room temperature for 1 min. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager as described in Experimental procedures . The alphabets shown in the figure indicate as follows: N, template base; A, adenine; C, cytosine; G, guanine; T, thymine; P, primer.

    Journal: Molecular Microbiology

    Article Title: Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains

    doi: 10.1111/mmi.12061

    Figure Lengend Snippet: Incorporation of 8-oxo-dGTP by pol III*. The Cy3-labelled 18-mer primer/36-mer template (sequences 1, 0.1 μM) was treated with pol III* (1 nM) in the presence of 100 μM 8-oxo-dGTP. The reaction mixtures were incubated at room temperature for 1 min. The samples were analysed by denaturing polyacrylamide gel electrophoresis and visualized by the Molecular Imager as described in Experimental procedures . The alphabets shown in the figure indicate as follows: N, template base; A, adenine; C, cytosine; G, guanine; T, thymine; P, primer.

    Article Snippet: Deoxyribonucleoside triphosphates (ultrapure grade) and 8-oxo-dGTP were purchased from Amersham Pharmacia Biotech (Buckinghamshire, UK) and TriLink BioTechnologies (San Diego, CA, USA) respectively.

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis