7h9 broth Difco Search Results


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  • 99
    Difco middlebrook 7h9 broth
    Middlebrook 7h9 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 1493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h9 broth
    Middlebrook 7h9 Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 broth medium
    7h9 Broth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 96/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h9 oadc broth
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middlebrook 7h9 Oadc Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebroook 7h9 broth
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middlebroook 7h9 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 7h9 broth medium
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    7h9 Broth Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middlebrook S 7h9 Broth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9 broth media
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middlebrook 7h9 Broth Media, supplied by Difco, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 broth
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    7h9 Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middlebrooke 7h9 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9 medium
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 97/100, based on 788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middelbrooks 7h9 broth
    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in <t>7H9-OADC-Tween</t> 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.
    Middelbrooks 7h9 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco mb 7h9 broth
    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB <t>7H9</t> medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.
    Mb 7h9 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals madc tw
    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB <t>7H9</t> medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.
    Madc Tw, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 80/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 medium
    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB <t>7H9</t> medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.
    7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 94/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middle brock 7h9 broth
    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB <t>7H9</t> medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.
    Middle Brock 7h9 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 albumin dextrose complex 7h9 adc broth
    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB <t>7H9</t> medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.
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    Difco middelbrook 7h9 broth medium
    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB <t>7H9</t> medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.
    Middelbrook 7h9 Broth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in 7H9-OADC-Tween 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.

    Journal: PLoS ONE

    Article Title: Gene Replacement in Mycobacterium chelonae: Application to the Construction of Porin Knock-Out Mutants

    doi: 10.1371/journal.pone.0094951

    Figure Lengend Snippet: Growth rates of wild-type M. chelonae ATCC 35752, its isogenic porin knock-out mutants and complemented MCH_4691 mutant strains in 7H9-OADC-Tween 80 broth at 30°C. Shown are representative results of two to three independent experiments using different culture batches.

    Article Snippet: M. chelonae strains ATCC 35752 and 9917 , and M. abscessus ATCC 19977 were grown in LB or Middlebrook 7H9-OADC broth (BD, Difco) supplemented with 0.05% Tween 80, 7H11-OADC agar (BD, Difco) or minimal Sauton's medium supplemented with 0.05% tyloxapol.

    Techniques: Knock-Out, Mutagenesis

    Gene replacement at the MCH_4689c , MCH_4690c and MCH_4691c porin loci of M. chelonae ATCC 35752 using the Ts-sacB and recombineering systems. (A) Porin gene cluster of M. chelonae ATCC 35752. The positions of the primers used to generate the allelic exchange substrates and analyze the candidate mutants are indicated. IGR1 and IGR2 represent the intergenic regions. (B) Candidate mutants obtained for each of the porin genes using the Ts-sacB or the recombineering systems were analyzed by PCR as described under Materials and Methods and confirmed by sequencing the regions flanking the resistance cassette. The expected size of the PCR fragments is 3.3 kb for the wild-type parent strain and 3.8 kb for the knock-out mutants. MWM, molecular weight marker. WT, wild-type. (C) Immunoblot analysis of porin production in the wild-type, mutant and complemented mutant strains. Strains were grown in 7H9-OADC-Tween 80 broth at 30°C to mid-log phase (OD600 = 1) and porins were selectively extracted from whole cells at 100°C using 0.5% n -octylpolyoxyethylene as a detergent as described [44] . Protein samples prepared from the same amount of cells for each strain were denatured by boiling in 80% DMSO followed by acetone precipitation [23] . Denatured proteins were loaded volume to volume, separated by SDS-PAGE, blotted onto a nitrocellulose membrane, and porins were detected using rabbit antiserum to purified MspA [23] . Immune complexes were detected by chemiluminescence (Pierce, ELC) and semi-quantified using the Image Lab software (Biorad).

    Journal: PLoS ONE

    Article Title: Gene Replacement in Mycobacterium chelonae: Application to the Construction of Porin Knock-Out Mutants

    doi: 10.1371/journal.pone.0094951

    Figure Lengend Snippet: Gene replacement at the MCH_4689c , MCH_4690c and MCH_4691c porin loci of M. chelonae ATCC 35752 using the Ts-sacB and recombineering systems. (A) Porin gene cluster of M. chelonae ATCC 35752. The positions of the primers used to generate the allelic exchange substrates and analyze the candidate mutants are indicated. IGR1 and IGR2 represent the intergenic regions. (B) Candidate mutants obtained for each of the porin genes using the Ts-sacB or the recombineering systems were analyzed by PCR as described under Materials and Methods and confirmed by sequencing the regions flanking the resistance cassette. The expected size of the PCR fragments is 3.3 kb for the wild-type parent strain and 3.8 kb for the knock-out mutants. MWM, molecular weight marker. WT, wild-type. (C) Immunoblot analysis of porin production in the wild-type, mutant and complemented mutant strains. Strains were grown in 7H9-OADC-Tween 80 broth at 30°C to mid-log phase (OD600 = 1) and porins were selectively extracted from whole cells at 100°C using 0.5% n -octylpolyoxyethylene as a detergent as described [44] . Protein samples prepared from the same amount of cells for each strain were denatured by boiling in 80% DMSO followed by acetone precipitation [23] . Denatured proteins were loaded volume to volume, separated by SDS-PAGE, blotted onto a nitrocellulose membrane, and porins were detected using rabbit antiserum to purified MspA [23] . Immune complexes were detected by chemiluminescence (Pierce, ELC) and semi-quantified using the Image Lab software (Biorad).

    Article Snippet: M. chelonae strains ATCC 35752 and 9917 , and M. abscessus ATCC 19977 were grown in LB or Middlebrook 7H9-OADC broth (BD, Difco) supplemented with 0.05% Tween 80, 7H11-OADC agar (BD, Difco) or minimal Sauton's medium supplemented with 0.05% tyloxapol.

    Techniques: Polymerase Chain Reaction, Sequencing, Knock-Out, Molecular Weight, Marker, Mutagenesis, SDS Page, Purification, Software

    MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB 7H9 medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.

    Journal: Journal of Bacteriology

    Article Title: Requirement of the mymA Operon for Appropriate Cell Wall Ultrastructure and Persistence of Mycobacterium tuberculosis in the Spleens of Guinea Pigs

    doi: 10.1128/JB.187.12.4173-4186.2005

    Figure Lengend Snippet: MALDI-TOF mass spectra of MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown in MB 7H9 medium at pH 7.0 or exposed to pH 5.0 for 12 h. MAMEs were extracted and subjected to MALDI-TOF analysis as described in Materials and Methods. MALDI-TOF mass spectra of MAMEs from M. tuberculosis (A and E), MtbΔ virS (B and F), Mtb mym :: hyg (C and G), and MtbΔ virS _ virS (D and H) are shown. The psuedomolecular masses of mycolates are indicated on each mass spectrum. Arrows and brackets indicate the major differences observed in MALDI-TOF analysis of MAMEs from various strains.

    Article Snippet: M. tuberculosis Erdman, the parental strain, mutant strains (MtbΔ virS and Mtb mym :: hyg ) and a virS -complemented strain (MtbΔ virS _ virS ) were grown in Middlebrook (MB) 7H9 broth (Difco Laboratories) supplemented with 0.5% glycerol, 0.2% Tween 80, and 1× ADC (albumin-dextrose complex; Difco Laboratories) or MB 7H10 medium (Difco Laboratories) supplemented with 1× OADC (oleic acid-albumin-dextrose complex; Difco Laboratories).

    Techniques:

    Effect of mymA disruption on the cell wall ultrastructure of M. tuberculosis . The cell wall of M. tuberculosis and Mtb mym :: hyg was examined by transmission electron microscopy. Cells were cultured in MB 7H9 medium to an A 600 ). Shown is electron microscopic analysis (×44,000) of (A) M. tuberculosis and (B) Mtb mym :: hyg . The photomicrographs shown are typical of the population as a whole, as judged by viewing many independent fields.

    Journal: Journal of Bacteriology

    Article Title: Requirement of the mymA Operon for Appropriate Cell Wall Ultrastructure and Persistence of Mycobacterium tuberculosis in the Spleens of Guinea Pigs

    doi: 10.1128/JB.187.12.4173-4186.2005

    Figure Lengend Snippet: Effect of mymA disruption on the cell wall ultrastructure of M. tuberculosis . The cell wall of M. tuberculosis and Mtb mym :: hyg was examined by transmission electron microscopy. Cells were cultured in MB 7H9 medium to an A 600 ). Shown is electron microscopic analysis (×44,000) of (A) M. tuberculosis and (B) Mtb mym :: hyg . The photomicrographs shown are typical of the population as a whole, as judged by viewing many independent fields.

    Article Snippet: M. tuberculosis Erdman, the parental strain, mutant strains (MtbΔ virS and Mtb mym :: hyg ) and a virS -complemented strain (MtbΔ virS _ virS ) were grown in Middlebrook (MB) 7H9 broth (Difco Laboratories) supplemented with 0.5% glycerol, 0.2% Tween 80, and 1× ADC (albumin-dextrose complex; Difco Laboratories) or MB 7H10 medium (Difco Laboratories) supplemented with 1× OADC (oleic acid-albumin-dextrose complex; Difco Laboratories).

    Techniques: Transmission Assay, Electron Microscopy, Cell Culture

    Representative HPLC chromatograms of mycolic acids from various strains of M. tuberculosis. M. tuberculosis MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were inoculated separately in MB 7H9 medium and grown to an A 600 of 1.5. Total fatty acids were extracted, derivatized to UV-absorbing p -bromophenacyl esters, and separated by HPLC as described in Materials and Methods. Shown are mycolic acid profiles of (A) M. tuberculosis , (B) MtbΔ virS , (C) Mtb mym :: hyg , and (D) MtbΔ virS _ virS from HPLC analysis. Peaks were labeled according to their RRTs. IS, internal high-molecular-weight standard. Arrows indicate the significant differences in the mycolic peaks between various strains.

    Journal: Journal of Bacteriology

    Article Title: Requirement of the mymA Operon for Appropriate Cell Wall Ultrastructure and Persistence of Mycobacterium tuberculosis in the Spleens of Guinea Pigs

    doi: 10.1128/JB.187.12.4173-4186.2005

    Figure Lengend Snippet: Representative HPLC chromatograms of mycolic acids from various strains of M. tuberculosis. M. tuberculosis MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were inoculated separately in MB 7H9 medium and grown to an A 600 of 1.5. Total fatty acids were extracted, derivatized to UV-absorbing p -bromophenacyl esters, and separated by HPLC as described in Materials and Methods. Shown are mycolic acid profiles of (A) M. tuberculosis , (B) MtbΔ virS , (C) Mtb mym :: hyg , and (D) MtbΔ virS _ virS from HPLC analysis. Peaks were labeled according to their RRTs. IS, internal high-molecular-weight standard. Arrows indicate the significant differences in the mycolic peaks between various strains.

    Article Snippet: M. tuberculosis Erdman, the parental strain, mutant strains (MtbΔ virS and Mtb mym :: hyg ) and a virS -complemented strain (MtbΔ virS _ virS ) were grown in Middlebrook (MB) 7H9 broth (Difco Laboratories) supplemented with 0.5% glycerol, 0.2% Tween 80, and 1× ADC (albumin-dextrose complex; Difco Laboratories) or MB 7H10 medium (Difco Laboratories) supplemented with 1× OADC (oleic acid-albumin-dextrose complex; Difco Laboratories).

    Techniques: High Performance Liquid Chromatography, Labeling, Molecular Weight

    Thin-layer chromatography of FAMEs and MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown to an A 600 of 1.5 in MB 7H9 medium, adjusted either to pH 7.0 or to pH 5.0, and labeled with 1 μCi/ml [1,2- 14 C]acetate for 12 h. Labeled lipids were extracted, derivatized to methyl esters, and resolved by normal phase TLC using petroleum ether/diethyl ether (17:3). Equal counts (200,000 cpm) were loaded in each lane, and a phosphorimager was used to quantify the proportion of FAMEs and MAMEs derived from various strains of M. tuberculosis . Shown are TLC profiles of total fatty acid esters (FAMEs and MAMEs) extracted from M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains growing at (A) neutral pH (7.0) or (B) acidic pH (5.0). These experiments were repeated four times, and similar results were obtained.

    Journal: Journal of Bacteriology

    Article Title: Requirement of the mymA Operon for Appropriate Cell Wall Ultrastructure and Persistence of Mycobacterium tuberculosis in the Spleens of Guinea Pigs

    doi: 10.1128/JB.187.12.4173-4186.2005

    Figure Lengend Snippet: Thin-layer chromatography of FAMEs and MAMEs from various strains of M. tuberculosis. M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains were grown to an A 600 of 1.5 in MB 7H9 medium, adjusted either to pH 7.0 or to pH 5.0, and labeled with 1 μCi/ml [1,2- 14 C]acetate for 12 h. Labeled lipids were extracted, derivatized to methyl esters, and resolved by normal phase TLC using petroleum ether/diethyl ether (17:3). Equal counts (200,000 cpm) were loaded in each lane, and a phosphorimager was used to quantify the proportion of FAMEs and MAMEs derived from various strains of M. tuberculosis . Shown are TLC profiles of total fatty acid esters (FAMEs and MAMEs) extracted from M. tuberculosis , MtbΔ virS , Mtb mym :: hyg , and MtbΔ virS _ virS strains growing at (A) neutral pH (7.0) or (B) acidic pH (5.0). These experiments were repeated four times, and similar results were obtained.

    Article Snippet: M. tuberculosis Erdman, the parental strain, mutant strains (MtbΔ virS and Mtb mym :: hyg ) and a virS -complemented strain (MtbΔ virS _ virS ) were grown in Middlebrook (MB) 7H9 broth (Difco Laboratories) supplemented with 0.5% glycerol, 0.2% Tween 80, and 1× ADC (albumin-dextrose complex; Difco Laboratories) or MB 7H10 medium (Difco Laboratories) supplemented with 1× OADC (oleic acid-albumin-dextrose complex; Difco Laboratories).

    Techniques: Thin Layer Chromatography, Labeling, Derivative Assay