Journal: Scientific Reports
Article Title: Universal stress protein Rv2624c alters abundance of arginine and enhances intracellular survival by ATP binding in mycobacteria
Figure Lengend Snippet: ATP binding by Rv2624c is required for its ability to increase survival in infected THP-1 cells. ( A ) The ATP-binding deficiencies of Rv2624c mutants did not influence growth on 7H10 medium. The mycobacterial strains indicated were serially diluted and were spotted onto solid 7H10 medium with 10% OADC. Pictures were taken after 3 d of incubation at 37 °C. ( B ) ATP-binding ability affects the survival of mycobacterial strains in THP-1 cells. Rv2624c mutant D17E with abrogated ATP-binding ability attenuated survival in THP-1 cells. The viability of Rv2624c mutant G109A, which had reduced ATP-binding ability, was comparable with that of wild-type Rv2624c. All results presented are representative of three independent experiments. p
Article Snippet: Reagents and growth media Mycobacterial strains were grown in liquid Middlebrook 7H9 medium (Becton Dickinson, Sparks, MD, USA) containing 10% OADC supplement (oleic acid, albumin, dextrose, catalase; Becton Dickinson, Sparks, MD, USA) and 0.05% Tween 80 (Sigma, St. Louis, MO, USA), or on solid 7H10 medium (Becton Dickinson, Sparks, MD, USA) containing 10% OADC supplement.
Techniques: Binding Assay, Infection, Incubation, Mutagenesis