7h10 solid medium Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Difco 7h10 solid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h10 Solid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid medium/product/Difco
    Average 91 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    7h10 solid medium - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    92
    Becton Dickinson 7h10 solid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h10 Solid Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid medium/product/Becton Dickinson
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    7h10 solid medium - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    95
    MiddleBrook Pharmaceuticals 7h10 solid medium
    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook <t>7H10</t> agar, and colonies
    7h10 Solid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid medium/product/MiddleBrook Pharmaceuticals
    Average 95 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    7h10 solid medium - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    94
    MiddleBrook Pharmaceuticals 7h10 solid growth medium
    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook <t>7H10</t> agar, and colonies
    7h10 Solid Growth Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid growth medium/product/MiddleBrook Pharmaceuticals
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    7h10 solid growth medium - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    84
    MiddleBrook Pharmaceuticals 7h10 solid medium plates
    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook <t>7H10</t> agar, and colonies
    7h10 Solid Medium Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid medium plates/product/MiddleBrook Pharmaceuticals
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    7h10 solid medium plates - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    93
    Difco 7h10 agar medium
    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook <t>7H10</t> agar, and colonies
    7h10 Agar Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 agar medium/product/Difco
    Average 93 stars, based on 118 article reviews
    Price from $9.99 to $1999.99
    7h10 agar medium - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    91
    MiddleBrook Pharmaceuticals 7h10 s7h11 solid medium biplates
    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook <t>7H10</t> agar, and colonies
    7h10 S7h11 Solid Medium Biplates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 s7h11 solid medium biplates/product/MiddleBrook Pharmaceuticals
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    7h10 s7h11 solid medium biplates - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    85
    MiddleBrook Pharmaceuticals solid medium
    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook <t>7H10</t> agar, and colonies
    Solid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid medium/product/MiddleBrook Pharmaceuticals
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    solid medium - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    93
    Difco middlebrook 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    Middlebrook 7h10 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10 medium/product/Difco
    Average 93 stars, based on 152 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 medium - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    94
    MiddleBrook Pharmaceuticals 7h10 panta supplemented medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    7h10 Panta Supplemented Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 panta supplemented medium/product/MiddleBrook Pharmaceuticals
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    7h10 panta supplemented medium - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    Becton Dickinson middlebrook 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    Middlebrook 7h10 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10 medium/product/Becton Dickinson
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 medium - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    86
    MiddleBrook Pharmaceuticals 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    7h10 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 medium/product/MiddleBrook Pharmaceuticals
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    7h10 medium - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    93
    Becton Dickinson 7h10 medium
    ATP binding by Rv2624c is required for its ability to increase survival in infected THP-1 cells. ( A ) The ATP-binding deficiencies of Rv2624c mutants did not influence growth on <t>7H10</t> medium. The mycobacterial strains indicated were serially diluted and were spotted onto solid 7H10 medium with 10% OADC. Pictures were taken after 3 d of incubation at 37 °C. ( B ) ATP-binding ability affects the survival of mycobacterial strains in THP-1 cells. Rv2624c mutant D17E with abrogated ATP-binding ability attenuated survival in THP-1 cells. The viability of Rv2624c mutant G109A, which had reduced ATP-binding ability, was comparable with that of wild-type Rv2624c. All results presented are representative of three independent experiments. p
    7h10 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 medium/product/Becton Dickinson
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    7h10 medium - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    MiddleBrook Pharmaceuticals 7h10 solid agar
    ATP binding by Rv2624c is required for its ability to increase survival in infected THP-1 cells. ( A ) The ATP-binding deficiencies of Rv2624c mutants did not influence growth on <t>7H10</t> medium. The mycobacterial strains indicated were serially diluted and were spotted onto solid 7H10 medium with 10% OADC. Pictures were taken after 3 d of incubation at 37 °C. ( B ) ATP-binding ability affects the survival of mycobacterial strains in THP-1 cells. Rv2624c mutant D17E with abrogated ATP-binding ability attenuated survival in THP-1 cells. The viability of Rv2624c mutant G109A, which had reduced ATP-binding ability, was comparable with that of wild-type Rv2624c. All results presented are representative of three independent experiments. p
    7h10 Solid Agar, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid agar/product/MiddleBrook Pharmaceuticals
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    7h10 solid agar - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    87
    MiddleBrook Pharmaceuticals 7h10 medium plates
    ATP binding by Rv2624c is required for its ability to increase survival in infected THP-1 cells. ( A ) The ATP-binding deficiencies of Rv2624c mutants did not influence growth on <t>7H10</t> medium. The mycobacterial strains indicated were serially diluted and were spotted onto solid 7H10 medium with 10% OADC. Pictures were taken after 3 d of incubation at 37 °C. ( B ) ATP-binding ability affects the survival of mycobacterial strains in THP-1 cells. Rv2624c mutant D17E with abrogated ATP-binding ability attenuated survival in THP-1 cells. The viability of Rv2624c mutant G109A, which had reduced ATP-binding ability, was comparable with that of wild-type Rv2624c. All results presented are representative of three independent experiments. p
    7h10 Medium Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 medium plates/product/MiddleBrook Pharmaceuticals
    Average 87 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    7h10 medium plates - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    97
    MiddleBrook Pharmaceuticals middlebrook 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    Middlebrook 7h10 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 97/100, based on 782 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10 medium/product/MiddleBrook Pharmaceuticals
    Average 97 stars, based on 782 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 medium - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    84
    MiddleBrook Pharmaceuticals middlebrook 7h10 oadc medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    Middlebrook 7h10 Oadc Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10 oadc medium/product/MiddleBrook Pharmaceuticals
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 oadc medium - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    7h10  (Difco)
    92
    Difco 7h10
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    7h10, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10/product/Difco
    Average 92 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    7h10 - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    91
    Millipore 7h10
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    7h10, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10/product/Millipore
    Average 91 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    7h10 - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    94
    Difco middlebrook 7h10 agar
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    Middlebrook 7h10 Agar, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10 agar/product/Difco
    Average 94 stars, based on 490 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 agar - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    95
    MiddleBrook Pharmaceuticals medium
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medium/product/MiddleBrook Pharmaceuticals
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    medium - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    97
    MiddleBrook Pharmaceuticals middlebrook 7h10
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    Middlebrook 7h10, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 97/100, based on 1205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10/product/MiddleBrook Pharmaceuticals
    Average 97 stars, based on 1205 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    93
    MiddleBrook Pharmaceuticals middlebrook 7h10 plates
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    Middlebrook 7h10 Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h10 plates/product/MiddleBrook Pharmaceuticals
    Average 93 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h10 plates - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    Becton Dickinson mb 7h10 agar
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    Mb 7h10 Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mb 7h10 agar/product/Becton Dickinson
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mb 7h10 agar - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    99
    MiddleBrook Pharmaceuticals 7h9 liquid medium
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid medium/product/MiddleBrook Pharmaceuticals
    Average 99 stars, based on 616 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid medium - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    87
    MiddleBrook Pharmaceuticals tween less middlebrook 7h10 broth
    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook <t>7H10</t> plates with or without 500 ng/ml ATc.
    Tween Less Middlebrook 7h10 Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween less middlebrook 7h10 broth/product/MiddleBrook Pharmaceuticals
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tween less middlebrook 7h10 broth - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    Image Search Results


    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Journal: Scientific Reports

    Article Title: Assessing the role of Rv1222 (RseA) as an anti-sigma factor of the Mycobacterium tuberculosis extracytoplasmic sigma factor SigE

    doi: 10.1038/s41598-019-41183-4

    Figure Lengend Snippet: Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Article Snippet: M. tuberculosis H37Rv was grown in either Middlebrook 7H9 liquid medium or Middlebrook 7H10 solid medium (Difco) supplemented with 0.05% Tween 80 and ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques: Mutagenesis, Incubation

    BCG harboring sigF :: lacZ translational fusion constructs give “target colony” phenotypes with sigF expression centrally. (Top) BCG harboring a control plasmid with the sigF :: lacZ fusion out of frame (pCK1107). (Middle) Integrated single-copy sigF :: lacZ fusion gene (pCK3215). (Bottom) Multicopy plasmid-borne sigF :: lacZ fusion (pCK3217). Colonies were grown on Middlebrook 7H10 agar supplemented with ADC, glycerol, cycloheximide, kanamycin, and X-Gal. After 5 weeks of incubation the colonies (each about 5 mm in diameter) were photographed.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Exposure to Antibiotics Induces Expression of the Mycobacterium tuberculosis sigF Gene: Implications for Chemotherapy against Mycobacterial Persistors

    doi:

    Figure Lengend Snippet: BCG harboring sigF :: lacZ translational fusion constructs give “target colony” phenotypes with sigF expression centrally. (Top) BCG harboring a control plasmid with the sigF :: lacZ fusion out of frame (pCK1107). (Middle) Integrated single-copy sigF :: lacZ fusion gene (pCK3215). (Bottom) Multicopy plasmid-borne sigF :: lacZ fusion (pCK3217). Colonies were grown on Middlebrook 7H10 agar supplemented with ADC, glycerol, cycloheximide, kanamycin, and X-Gal. After 5 weeks of incubation the colonies (each about 5 mm in diameter) were photographed.

    Article Snippet: Transformations into Mycobacterium bovis BCG and Mycobacterium smegmatis were performed as described previously ( , ) with a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, Calif.) and by allowing recovery for 24 or 3 h, respectively, in Middlebrook 7H9 liquid medium (Difco Laboratories, Detroit, Mich.) supplemented with albumin-dextrose complex (ADC) and Tween 80 , prior to plating on Middlebrook 7H10 solid selective medium (Difco Laboratories).

    Techniques: Construct, Expressing, Plasmid Preparation, Incubation

    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook 7H10 agar, and colonies

    Journal: Infection and Immunity

    Article Title: Individual Mycobacterium tuberculosis Resuscitation-Promoting Factor Homologues Are Dispensable for Growth In Vitro and In Vivo

    doi: 10.1128/IAI.72.1.515-526.2004

    Figure Lengend Snippet: Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook 7H10 agar, and colonies

    Article Snippet: When plated onto Middlebrook 7H10 solid medium, each of the rpf deletion mutant strains displayed a colony morphology similar to wild type, with the exception of the Rv1009 disruption mutant strains, mc2 3126 and mc2 3126A.

    Techniques:

    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on Middlebrook 7H10 agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P

    Journal: BMC Immunology

    Article Title: Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection

    doi: 10.1186/s12865-018-0258-8

    Figure Lengend Snippet: BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on Middlebrook 7H10 agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P

    Article Snippet: Both strains were grown with gentle agitation (80 rpm) in Middlebrook 7H9 medium (Difco, Detroit, MI, USA) supplemented with 0.05% Tween 80 and 10% albumin-dextrose-catalase (ADC) enrichment or on solid Middlebrook 7H10 medium (Difco) supplemented with 0.05% Tween 80 and 10% oleic-ADC enrichment.

    Techniques: Infection, Mouse Assay, Injection

    ATP binding by Rv2624c is required for its ability to increase survival in infected THP-1 cells. ( A ) The ATP-binding deficiencies of Rv2624c mutants did not influence growth on 7H10 medium. The mycobacterial strains indicated were serially diluted and were spotted onto solid 7H10 medium with 10% OADC. Pictures were taken after 3 d of incubation at 37 °C. ( B ) ATP-binding ability affects the survival of mycobacterial strains in THP-1 cells. Rv2624c mutant D17E with abrogated ATP-binding ability attenuated survival in THP-1 cells. The viability of Rv2624c mutant G109A, which had reduced ATP-binding ability, was comparable with that of wild-type Rv2624c. All results presented are representative of three independent experiments. p

    Journal: Scientific Reports

    Article Title: Universal stress protein Rv2624c alters abundance of arginine and enhances intracellular survival by ATP binding in mycobacteria

    doi: 10.1038/srep35462

    Figure Lengend Snippet: ATP binding by Rv2624c is required for its ability to increase survival in infected THP-1 cells. ( A ) The ATP-binding deficiencies of Rv2624c mutants did not influence growth on 7H10 medium. The mycobacterial strains indicated were serially diluted and were spotted onto solid 7H10 medium with 10% OADC. Pictures were taken after 3 d of incubation at 37 °C. ( B ) ATP-binding ability affects the survival of mycobacterial strains in THP-1 cells. Rv2624c mutant D17E with abrogated ATP-binding ability attenuated survival in THP-1 cells. The viability of Rv2624c mutant G109A, which had reduced ATP-binding ability, was comparable with that of wild-type Rv2624c. All results presented are representative of three independent experiments. p

    Article Snippet: Reagents and growth media Mycobacterial strains were grown in liquid Middlebrook 7H9 medium (Becton Dickinson, Sparks, MD, USA) containing 10% OADC supplement (oleic acid, albumin, dextrose, catalase; Becton Dickinson, Sparks, MD, USA) and 0.05% Tween 80 (Sigma, St. Louis, MO, USA), or on solid 7H10 medium (Becton Dickinson, Sparks, MD, USA) containing 10% OADC supplement.

    Techniques: Binding Assay, Infection, Incubation, Mutagenesis

    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on Middlebrook 7H10 agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P

    Journal: BMC Immunology

    Article Title: Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection

    doi: 10.1186/s12865-018-0258-8

    Figure Lengend Snippet: BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on Middlebrook 7H10 agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P

    Article Snippet: Ten-fold serial dilutions of these suspensions were then plated on solid Middlebrook 7H10 medium, and colonies were counted after incubation at 37 °C for 2–3 weeks.

    Techniques: Infection, Mouse Assay, Injection

    pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook 7H10 plates with or without 500 ng/ml ATc.

    Journal: Journal of Bacteriology

    Article Title: The Phosphatidyl-myo-Inositol Mannosyltransferase PimA Is Essential for Mycobacterium tuberculosis Growth In Vitro and In Vivo

    doi: 10.1128/JB.01346-13

    Figure Lengend Snippet: pimA is an essential gene. Five microliters of a suspension of TB101 was spotted at the indicated dilutions on Middlebrook 7H10 plates with or without 500 ng/ml ATc.

    Article Snippet: M. tuberculosis H37Rv and its derivative, TB38, were grown at 37°C in Middlebrook 7H9 (liquid medium) or 7H10 (solid medium) (Difco) supplemented with 0.05% (vol/vol) Tween 80 (Sigma-Aldrich), 0.2% (vol/vol) glycerol (Sigma-Aldrich), and 10% ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques:

    (A) Growth curves of TB101 in Middlebrook 7H9 containing different concentrations of ATc. The optical density at 540 nm was recorded at different time points and used to compile the growth curves. (B) Killing curves of the pimA conditional mutant TB101 in the presence of ATc. The conditional mutant was grown in Middlebrook 7H9 containing 200 ng/ml ATc. Starting from day 1, samples were collected at different time points and plated on Middlebrook 7H10 to determine the viable counts. Each experiment was repeated at least twice, giving similar results. Filled squares, optical density; open squares, viable counts.

    Journal: Journal of Bacteriology

    Article Title: The Phosphatidyl-myo-Inositol Mannosyltransferase PimA Is Essential for Mycobacterium tuberculosis Growth In Vitro and In Vivo

    doi: 10.1128/JB.01346-13

    Figure Lengend Snippet: (A) Growth curves of TB101 in Middlebrook 7H9 containing different concentrations of ATc. The optical density at 540 nm was recorded at different time points and used to compile the growth curves. (B) Killing curves of the pimA conditional mutant TB101 in the presence of ATc. The conditional mutant was grown in Middlebrook 7H9 containing 200 ng/ml ATc. Starting from day 1, samples were collected at different time points and plated on Middlebrook 7H10 to determine the viable counts. Each experiment was repeated at least twice, giving similar results. Filled squares, optical density; open squares, viable counts.

    Article Snippet: M. tuberculosis H37Rv and its derivative, TB38, were grown at 37°C in Middlebrook 7H9 (liquid medium) or 7H10 (solid medium) (Difco) supplemented with 0.05% (vol/vol) Tween 80 (Sigma-Aldrich), 0.2% (vol/vol) glycerol (Sigma-Aldrich), and 10% ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques: Mutagenesis

    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Journal: Frontiers in Microbiology

    Article Title: Functional Studies of Five Toxin-Antitoxin Modules in Mycobacterium tuberculosis H37Rv

    doi: 10.3389/fmicb.2016.02071

    Figure Lengend Snippet: Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Article Snippet: M. smegmatis mc2 155 was grown in Middlebrook 7H9 (a liquid medium, Difco) containing 0.2% glycerol and 0.02% Tween 80 or Middlebrook 7H10 (a solid agar medium, Difco) containing 0.2% glycerol.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation