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    Thermo Fisher 7900ht fast realtime pcr system
    mRNA levels of SPARC and SLUG in melanoma cells at various stages of tumor development. ( A ) Analysis of SPARC and SLUG mRNA levels: relative gene expression levels of SPARC and SLUG in cultures of melanoma cells derived from RGP, VGP or metastatic melanoma tumors were evaluated by relative <t>Q-PCR</t> using an ABI Biosystems <t>7900HT</t> Fast Real Time PCR System and the SYBR Green dye detection protocol. Data were analyzed using the 2 −ddCt method and human 18S transcript level was used to normalize for each sample. Values are the mean of independent triplicates. RGP, Radial Growth Phase; VGP, Vertical Growth Phase. ( B ) Positive correlation between SPARC and SLUG in melanoma samples: regression analysis to determine the correlation between SPARC and SLUG in human melanoma samples. R , Spearman’s Rank Correlation Coefficient; P
    7900ht Fast Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 28587 article reviews
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    7900ht fast realtime pcr system - by Bioz Stars, 2020-08
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    mRNA levels of SPARC and SLUG in melanoma cells at various stages of tumor development. ( A ) Analysis of SPARC and SLUG mRNA levels: relative gene expression levels of SPARC and SLUG in cultures of melanoma cells derived from RGP, VGP or metastatic melanoma tumors were evaluated by relative Q-PCR using an ABI Biosystems 7900HT Fast Real Time PCR System and the SYBR Green dye detection protocol. Data were analyzed using the 2 −ddCt method and human 18S transcript level was used to normalize for each sample. Values are the mean of independent triplicates. RGP, Radial Growth Phase; VGP, Vertical Growth Phase. ( B ) Positive correlation between SPARC and SLUG in melanoma samples: regression analysis to determine the correlation between SPARC and SLUG in human melanoma samples. R , Spearman’s Rank Correlation Coefficient; P

    Journal: PLoS ONE

    Article Title: The Epithelial-Mesenchymal Transition (EMT) Regulatory Factor SLUG (SNAI2) Is a Downstream Target of SPARC and AKT in Promoting Melanoma Cell Invasion

    doi: 10.1371/journal.pone.0040378

    Figure Lengend Snippet: mRNA levels of SPARC and SLUG in melanoma cells at various stages of tumor development. ( A ) Analysis of SPARC and SLUG mRNA levels: relative gene expression levels of SPARC and SLUG in cultures of melanoma cells derived from RGP, VGP or metastatic melanoma tumors were evaluated by relative Q-PCR using an ABI Biosystems 7900HT Fast Real Time PCR System and the SYBR Green dye detection protocol. Data were analyzed using the 2 −ddCt method and human 18S transcript level was used to normalize for each sample. Values are the mean of independent triplicates. RGP, Radial Growth Phase; VGP, Vertical Growth Phase. ( B ) Positive correlation between SPARC and SLUG in melanoma samples: regression analysis to determine the correlation between SPARC and SLUG in human melanoma samples. R , Spearman’s Rank Correlation Coefficient; P

    Article Snippet: Quantitative PCR was performed on 25 ng cDNA samples, in sealed 384-well microtiter plates using the SYBR Green™ PCR Master Mix (Applied Biosystems) with the 7900HT Fast Real-Time PCR System (Applied Biosystems).

    Techniques: Expressing, Derivative Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, SYBR Green Assay

    hKLK1-MSCs transfer down-regulated the expression of inflammatory cytokines and apoptosis associated cytokines in mouse kidney. The expression of 13 genes representing proinflammatory cytokines, chemokines and apoptotic factors in the kidneys were measured by QPCR in B6.Sle1.Sle3 mice treated with PBS, hKLK1-MSCs or vector-MSCs. Total renal RNA was extracted 28 days after treatment. Taqman assay was performed using an ABI 7900HT real-time PCR system. RQ (relative quantity) represents the mean of 5 samples per group. Error bars denote SD.

    Journal: PLoS ONE

    Article Title: Kallikrein Transduced Mesenchymal Stem Cells Protect against Anti-GBM Disease and Lupus Nephritis by Ameliorating Inflammation and Oxidative Stress

    doi: 10.1371/journal.pone.0067790

    Figure Lengend Snippet: hKLK1-MSCs transfer down-regulated the expression of inflammatory cytokines and apoptosis associated cytokines in mouse kidney. The expression of 13 genes representing proinflammatory cytokines, chemokines and apoptotic factors in the kidneys were measured by QPCR in B6.Sle1.Sle3 mice treated with PBS, hKLK1-MSCs or vector-MSCs. Total renal RNA was extracted 28 days after treatment. Taqman assay was performed using an ABI 7900HT real-time PCR system. RQ (relative quantity) represents the mean of 5 samples per group. Error bars denote SD.

    Article Snippet: QPCR were carried out on a 7900HT real-time PCR system (Applied Biosystems) with the universal PCR reaction system under standard thermal cycler conditions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Plasmid Preparation, TaqMan Assay