7500 real time pcr system Thermo Fisher Search Results


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  • 90
    Thermo Fisher real time pcr system
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Thermo Fisher 7500 real time pcr
    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Thermo Fisher real time pcr machine
    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Thermo Fisher real‑time pcr system
    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
    Quantstudio Real Time Pcr Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with <t>qRT-PCR.</t> The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p
    Abi 7500 Real Time Pcr System Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Prox1 is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by PCR. Data represent one of three separate experiments.

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Prox1 is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by PCR. Data represent one of three separate experiments.

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Sequencing, Polymerase Chain Reaction

    Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Over Expression, Expressing, Plasmid Preparation, Generated, Infection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection

    Expression of Prox1 mRNA and protein in T cells ( A, B and C ) PBMCs, naïve CD4 + T cells and Jurkat cells were isolated, and stimulated with PHA and anti-CD3/CD28 Dynabeads for 24 h respectively. (A) Prox1 and (C) IL-2 mRNA levels were measured by RT-PCR, while (B) Prox1 protein levels were assessed by Western blot. For (A), (B) and (C), the data represent one of three independent experiments. ( D and E ) Naïve CD4 + T cells were stimulated with anti-CD3/CD28 Dynabeads for indicated times. (D) Prox1 and (E) IL-2 mRNA levels were measured by real-time PCR. Gene expression is normalized against the amount of GAPDH mRNA. The data from represent the mean ± SEM of three experiments.

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Expression of Prox1 mRNA and protein in T cells ( A, B and C ) PBMCs, naïve CD4 + T cells and Jurkat cells were isolated, and stimulated with PHA and anti-CD3/CD28 Dynabeads for 24 h respectively. (A) Prox1 and (C) IL-2 mRNA levels were measured by RT-PCR, while (B) Prox1 protein levels were assessed by Western blot. For (A), (B) and (C), the data represent one of three independent experiments. ( D and E ) Naïve CD4 + T cells were stimulated with anti-CD3/CD28 Dynabeads for indicated times. (D) Prox1 and (E) IL-2 mRNA levels were measured by real-time PCR. Gene expression is normalized against the amount of GAPDH mRNA. The data from represent the mean ± SEM of three experiments.

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction

    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative PCR (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The SUR2B subunit of rat vascular KATP channel is targeted by miR-9a-3p induced by prolonged exposure to methylglyoxal

    doi: 10.1152/ajpcell.00311.2014

    Figure Lengend Snippet: Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative PCR (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P

    Article Snippet: The qPCR was performed with a Fast Real-time PCR system (Applied Biosystems 7500) for 40 cycles.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with qRT-PCR. The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p

    Journal: Development & Reproduction

    Article Title: Expression Analysis of Interferon-Stimulated Gene 15 in the Rock Bream Oplegnathus fasciatus against Rock Bream Iridovirus (RSIV) Challenge

    doi: 10.12717/DR.2017.21.4.371

    Figure Lengend Snippet: Temporal distribution of ISG15 mRNA in various tissues following RSIV infection. (A) The expression level of ISG15 was determined in the (A) kidney, (B) muscle, and (C) spleen with qRT-PCR. The samples were analyzed at 0, 3, 6, 12, 24, and 72 hours post-injection. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p

    Article Snippet: Quantitative real-time PCR (qRT-PCR) qRT-PCR (ABI 7500, Applied Biosystems) was performed on rock bream samples to quantify the expression of ISG15.

    Techniques: Infection, Expressing, Quantitative RT-PCR, Injection

    Temporal expression analysis of ISG15 following rock bream iridovirus (RSIV) infection in whole fish. (A) Reverse transcription (RT)-PCR was performed with 1 μg of total RNA using ISG15-qRT-1F and 1R primers. Beta-actin was included in all reactions to verify equal complementary DNA concentrations in the PCR reaction and on the gel. (B) qRT-PCR analysis of ISG15 in rock bream following RSIV infection at 0, 3, 6, 12, 24, and 72 h. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p

    Journal: Development & Reproduction

    Article Title: Expression Analysis of Interferon-Stimulated Gene 15 in the Rock Bream Oplegnathus fasciatus against Rock Bream Iridovirus (RSIV) Challenge

    doi: 10.12717/DR.2017.21.4.371

    Figure Lengend Snippet: Temporal expression analysis of ISG15 following rock bream iridovirus (RSIV) infection in whole fish. (A) Reverse transcription (RT)-PCR was performed with 1 μg of total RNA using ISG15-qRT-1F and 1R primers. Beta-actin was included in all reactions to verify equal complementary DNA concentrations in the PCR reaction and on the gel. (B) qRT-PCR analysis of ISG15 in rock bream following RSIV infection at 0, 3, 6, 12, 24, and 72 h. Each experiment was performed in triplicate and the expression levels of beta actin and ISG15 at 0 h were set as 1. Asterisks indicate statistically significant differences ( * p

    Article Snippet: Quantitative real-time PCR (qRT-PCR) qRT-PCR (ABI 7500, Applied Biosystems) was performed on rock bream samples to quantify the expression of ISG15.

    Techniques: Expressing, Infection, Fluorescence In Situ Hybridization, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Quantitative RT-PCR