Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2
Figure Lengend Snippet: Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P
Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.
Techniques: Over Expression, Expressing, Plasmid Preparation, Generated, Infection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection