7500 fast real-time pcr system Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher 7500 fast realtime pcr system
    Upregulation of USP5 and its role in in pancreatic cancer USP5 mRNA expression was quantified by <t>realtime</t> <t>PCR</t> (qRT-PCR) and normalized to ribosomal protein, large, P0 (RPLP0) mRNA levels. (A) Expression in malignant tissues (n=10) was significantly increased compared to healthy controls (n=8) or chronic pancreatitis tissues (n=6). CP = Chronic Pancreatitis. (B, C) Transient loss of USP5 function significantly reduced proliferation and viability of four different pancreatic cancer cell lines as assessed by their ability to metabolize MTT reagent (B, viability index) or incorporate BrdU agent (C, proliferation index) as compared to non-silencing control siRNA (siC) or untreated cells (NT). (D) USP5 knockdown however had no impact on the migration speed of PaTu-8988T cells, as assessed by Time Lapse Microscopy and automated cell tracking. si1, si2 and si3 = three specific siRNAs against USP5 . siC = non-silencing control. NT = untreated cells. *p
    7500 Fast Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7500 fast realtime pcr system/product/Thermo Fisher
    Average 99 stars, based on 4409 article reviews
    Price from $9.99 to $1999.99
    7500 fast realtime pcr system - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher fast 7500 realtime pcr systems
    Activation of STAT1 and STAT3 by U1-snRNA. ( A and B ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After the indicated time periods, cellular content of pIRF3 (A and B), pSTAT1 (A) and pSTAT3 (B) was determined by immunoblot analysis. For detection of pIRF3 and pSTAT1/3 on the same blot, blots were cut in half. For each experimental setup, one representative of three independently performed experiments is shown. ( C ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After 8 h, cellular IL-18BP mRNA expression was assessed by quantitative <t>realtime</t> <t>PCR</t> analysis. IL-18BP mRNA was normalized to that of GAPDH and is shown as fold induction compared with mock-stimulated control ± SD ( n = 3); ** P
    Fast 7500 Realtime Pcr Systems, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast 7500 realtime pcr systems/product/Thermo Fisher
    Average 99 stars, based on 7778 article reviews
    Price from $9.99 to $1999.99
    fast 7500 realtime pcr systems - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher 7500 7500 fast real time pcr system
    Activation of STAT1 and STAT3 by U1-snRNA. ( A and B ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After the indicated time periods, cellular content of pIRF3 (A and B), pSTAT1 (A) and pSTAT3 (B) was determined by immunoblot analysis. For detection of pIRF3 and pSTAT1/3 on the same blot, blots were cut in half. For each experimental setup, one representative of three independently performed experiments is shown. ( C ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After 8 h, cellular IL-18BP mRNA expression was assessed by quantitative <t>realtime</t> <t>PCR</t> analysis. IL-18BP mRNA was normalized to that of GAPDH and is shown as fold induction compared with mock-stimulated control ± SD ( n = 3); ** P
    7500 7500 Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7500 7500 fast real time pcr system/product/Thermo Fisher
    Average 91 stars, based on 520 article reviews
    Price from $9.99 to $1999.99
    7500 7500 fast real time pcr system - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Upregulation of USP5 and its role in in pancreatic cancer USP5 mRNA expression was quantified by realtime PCR (qRT-PCR) and normalized to ribosomal protein, large, P0 (RPLP0) mRNA levels. (A) Expression in malignant tissues (n=10) was significantly increased compared to healthy controls (n=8) or chronic pancreatitis tissues (n=6). CP = Chronic Pancreatitis. (B, C) Transient loss of USP5 function significantly reduced proliferation and viability of four different pancreatic cancer cell lines as assessed by their ability to metabolize MTT reagent (B, viability index) or incorporate BrdU agent (C, proliferation index) as compared to non-silencing control siRNA (siC) or untreated cells (NT). (D) USP5 knockdown however had no impact on the migration speed of PaTu-8988T cells, as assessed by Time Lapse Microscopy and automated cell tracking. si1, si2 and si3 = three specific siRNAs against USP5 . siC = non-silencing control. NT = untreated cells. *p

    Journal: Oncotarget

    Article Title: The deubiquitinating enzyme USP5 promotes pancreatic cancer via modulating cell cycle regulators

    doi: 10.18632/oncotarget.19882

    Figure Lengend Snippet: Upregulation of USP5 and its role in in pancreatic cancer USP5 mRNA expression was quantified by realtime PCR (qRT-PCR) and normalized to ribosomal protein, large, P0 (RPLP0) mRNA levels. (A) Expression in malignant tissues (n=10) was significantly increased compared to healthy controls (n=8) or chronic pancreatitis tissues (n=6). CP = Chronic Pancreatitis. (B, C) Transient loss of USP5 function significantly reduced proliferation and viability of four different pancreatic cancer cell lines as assessed by their ability to metabolize MTT reagent (B, viability index) or incorporate BrdU agent (C, proliferation index) as compared to non-silencing control siRNA (siC) or untreated cells (NT). (D) USP5 knockdown however had no impact on the migration speed of PaTu-8988T cells, as assessed by Time Lapse Microscopy and automated cell tracking. si1, si2 and si3 = three specific siRNAs against USP5 . siC = non-silencing control. NT = untreated cells. *p

    Article Snippet: Quantitative real time Reverse Transcription PCR (qRT-PCR) was performed using SYBR Green MasterMix (Applied Biosystems, USA) on a 7500 Fast Realtime PCR system (Applied Biosystems).

    Techniques: Expressing, Polymerase Chain Reaction, Quantitative RT-PCR, MTT Assay, Migration, Time-lapse Microscopy, Cell Tracking Assay

    STING N153S triggers perivascular pulmonary inflammation, myeloid cell expansion, and T cell cytopenia independently of IRF3. (A) Expression of ISGs in STING N153S, STING knockout ( Sting −/− ), and Trex1 −/− MEFs as well as STING N153S and Sting −/− spleens. Each data point represents a unique ISG as fold increase compared with a WT littermate control. Data were assembled using ISG expression values from RNA-sequencing. (B) qRT-PCR analysis of ISGs in WT, STING N153S, Sting −/− MEFs. mRNA expression is normalized to WT. (C) ISG expression in two healthy control (HC) and SAVI patient skin fibroblasts. Data represent a selection of ISGs assembled from RNA-sequencing. (D) qRT-PCR analysis of ISGs in skin fibroblasts. Data are from two independent experiments with two technical replicates per ISG, normalized to HC. (E) Hematoxylin and eosin images of lungs from Irf3 −/− and Irf3 −/− STING N153S mice (4–6 mo of age). Pulmonary vessels are indicated (v) with black arrows highlighting perivascular immune cell infiltration. Pulmonary vessels are indicated (v) with arrows. Bar, 100 µm. (F) Quantitation of perivascular lung lesions in STING N153S ( n = 7) and Irf3 −/− N153S ( n = 4) mice. Results in F represent the mean ± SEM of data collected and analyzed in two independent experiments. (G) ISG mRNA expression in Irf3 −/− and Irf3 −/− N153S MEFs normalized to gene expression in Irf3 −/− MEFs. Data represent the mean ± SEM from two independent experiments with n = 3 biological replicates per genotype. (H–J) Representative dot plots (H) and quantification (I and J) of splenic myeloid cell populations from WT, STING N153S, Irf3 −/− , and Irf3 −/− STING N153S mice. Data represent the mean of two independent experiments with n = 4 or 5 mice per genotype. (K and L) Percentage (K) and total number (L) of CD8 + splenocytes from WT, STING N153S, Irf3 −/− , and Irf3 −/− STING N153S mice. Data were pooled from two independent experiments with n = 4 or 5 mice per genotype. ns, not significant; *, P

    Journal: The Journal of Experimental Medicine

    Article Title: STING-associated vasculopathy develops independently of IRF3 in mice

    doi: 10.1084/jem.20171351

    Figure Lengend Snippet: STING N153S triggers perivascular pulmonary inflammation, myeloid cell expansion, and T cell cytopenia independently of IRF3. (A) Expression of ISGs in STING N153S, STING knockout ( Sting −/− ), and Trex1 −/− MEFs as well as STING N153S and Sting −/− spleens. Each data point represents a unique ISG as fold increase compared with a WT littermate control. Data were assembled using ISG expression values from RNA-sequencing. (B) qRT-PCR analysis of ISGs in WT, STING N153S, Sting −/− MEFs. mRNA expression is normalized to WT. (C) ISG expression in two healthy control (HC) and SAVI patient skin fibroblasts. Data represent a selection of ISGs assembled from RNA-sequencing. (D) qRT-PCR analysis of ISGs in skin fibroblasts. Data are from two independent experiments with two technical replicates per ISG, normalized to HC. (E) Hematoxylin and eosin images of lungs from Irf3 −/− and Irf3 −/− STING N153S mice (4–6 mo of age). Pulmonary vessels are indicated (v) with black arrows highlighting perivascular immune cell infiltration. Pulmonary vessels are indicated (v) with arrows. Bar, 100 µm. (F) Quantitation of perivascular lung lesions in STING N153S ( n = 7) and Irf3 −/− N153S ( n = 4) mice. Results in F represent the mean ± SEM of data collected and analyzed in two independent experiments. (G) ISG mRNA expression in Irf3 −/− and Irf3 −/− N153S MEFs normalized to gene expression in Irf3 −/− MEFs. Data represent the mean ± SEM from two independent experiments with n = 3 biological replicates per genotype. (H–J) Representative dot plots (H) and quantification (I and J) of splenic myeloid cell populations from WT, STING N153S, Irf3 −/− , and Irf3 −/− STING N153S mice. Data represent the mean of two independent experiments with n = 4 or 5 mice per genotype. (K and L) Percentage (K) and total number (L) of CD8 + splenocytes from WT, STING N153S, Irf3 −/− , and Irf3 −/− STING N153S mice. Data were pooled from two independent experiments with n = 4 or 5 mice per genotype. ns, not significant; *, P

    Article Snippet: RNA was quantified and quality confirmed using a NanoDrop ND-1000 spectrophotometer. cDNA was synthesized with iScript cDNA synthesis kit (Bio-Rad). iTaq Universal SYBR Green Supermix (Bio-Rad) and an ABI-7500 Fast Real-Time PCR system (Applied Biosystems) were used for qRT-PCR.

    Techniques: Expressing, Knock-Out, RNA Sequencing Assay, Quantitative RT-PCR, Selection, Mouse Assay, Quantitation Assay

    Decreased thymic CD4 output was observed in young but not middle-aged donors. (A) TREC counts (left) and CD31 + percentages in naïve CD4 T cells (right) of young and middle-aged donors. PBMC were isolated from leukapheresis blood and CD4 T cells were further isolated via positive selection. TREC content was examined longitudinally by Taqman Real time PCR. CD31 expression was determined by flow cytometry analysis. The average of all time points were presented with the SD ( P ≤ 0.05). (B) TREC frequencies in CD4 T cells isolated from young (left panels) and middle-aged (right panels) donors are shown. (C) CD31 + CD45RA + CD4 T cells.

    Journal: Frontiers in Immunology

    Article Title: Homeostasis of lymphocytes and monocytes in frequent blood donors

    doi: 10.3389/fimmu.2012.00271

    Figure Lengend Snippet: Decreased thymic CD4 output was observed in young but not middle-aged donors. (A) TREC counts (left) and CD31 + percentages in naïve CD4 T cells (right) of young and middle-aged donors. PBMC were isolated from leukapheresis blood and CD4 T cells were further isolated via positive selection. TREC content was examined longitudinally by Taqman Real time PCR. CD31 expression was determined by flow cytometry analysis. The average of all time points were presented with the SD ( P ≤ 0.05). (B) TREC frequencies in CD4 T cells isolated from young (left panels) and middle-aged (right panels) donors are shown. (C) CD31 + CD45RA + CD4 T cells.

    Article Snippet: The frequency of the δRec-ψJα TREC created when the TCRD segment is excised from the TCR β gene locus was determined using Taqman real-time PCR 7500 Fast Real-Time PCR System (Applied Biosystems).

    Techniques: Isolation, Selection, Real-time Polymerase Chain Reaction, Expressing, Flow Cytometry, Cytometry

    Quantitative mRNA analysis of cytokine expression in intestinal IELs. Total RNA was extracted from freshly isolated IELs of BALB/c and DO11.10 mice ( N = 5), and cDNA of IL-2, IL-4, IL-6, IL-10, IL-17, IFN- γ , TNF- α , and TGB- β was made using PureLink Micro-to-Midi Total RNA Purification System (Invitrogen, SP, Brazil,). Quantitative PCR analysis was performed with the ABI 7500 Fast Real-Time PCR system (Applied Biosystems), in four replicates, with the TaqMan Mastermix (Applied Biosystems). Samples were normalized to 18S rRNA, and an arbitrary value of 1 was given to control group (naïve mice) for the normalization, and the remaining samples were plotted relative to that value. IELs from BALB/c mice immunized by ip route show an increase in the expression of mRNA of cytokines IL-10, IL-2, IFN- γ , TGF- β , and TNF- α . IELs from DO11.10 mice fed with OVA and challenged by ip route showed increased expression of mRNA for cytokines IFN- γ , TGF- β , and TNF- α . The results are representative of three independent experiments.

    Journal: Clinical and Developmental Immunology

    Article Title: Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance

    doi: 10.1155/2012/208054

    Figure Lengend Snippet: Quantitative mRNA analysis of cytokine expression in intestinal IELs. Total RNA was extracted from freshly isolated IELs of BALB/c and DO11.10 mice ( N = 5), and cDNA of IL-2, IL-4, IL-6, IL-10, IL-17, IFN- γ , TNF- α , and TGB- β was made using PureLink Micro-to-Midi Total RNA Purification System (Invitrogen, SP, Brazil,). Quantitative PCR analysis was performed with the ABI 7500 Fast Real-Time PCR system (Applied Biosystems), in four replicates, with the TaqMan Mastermix (Applied Biosystems). Samples were normalized to 18S rRNA, and an arbitrary value of 1 was given to control group (naïve mice) for the normalization, and the remaining samples were plotted relative to that value. IELs from BALB/c mice immunized by ip route show an increase in the expression of mRNA of cytokines IL-10, IL-2, IFN- γ , TGF- β , and TNF- α . IELs from DO11.10 mice fed with OVA and challenged by ip route showed increased expression of mRNA for cytokines IFN- γ , TGF- β , and TNF- α . The results are representative of three independent experiments.

    Article Snippet: The cDNA was made using SuperScript III First-Strand Synthesis Supermix (Invitrogen) with random primers (Invitrogen) and analyzed for IL-2, IL-4, IL-10, IL-6, IL-17, IFN-γ , TGF-β , and TNF-α gene expression by real-time PCR assay using an 7500 Fast Real-Time PCR (Applied Biosystems, Foster City, CA) according to the manufacturer's instructions; 18S ribosomal RNA (rRNA) was used as an internal control.

    Techniques: Expressing, Isolation, Mouse Assay, Purification, Real-time Polymerase Chain Reaction

    U1 small nuclear RNA (snRNA) selectively enriched in the insoluble fraction from Alzheimer’s disease (AD) brain homogenates A . Dot blot of 10 μg insoluble fraction from three controls and three AD cases. Ponceau S stain and 2,2,7-trimethylguanosine cap labeling is shown. B–F . Quantitative real-time PCR for U1, U2, U4, U5 and U6 using cDNA reverse transcribed from control and AD insoluble fraction RNA. Average bar is show in each panel. 2^(-Delta delta Ct) values are shown. n = 4. *Statistical significance ( P = 0.003, Student’s t -test).

    Journal: Brain pathology (Zurich, Switzerland)

    Article Title: Aggregates of Small Nuclear Ribonucleic Acids (snRNAs) in Alzheimer’s Disease’

    doi: 10.1111/bpa.12133

    Figure Lengend Snippet: U1 small nuclear RNA (snRNA) selectively enriched in the insoluble fraction from Alzheimer’s disease (AD) brain homogenates A . Dot blot of 10 μg insoluble fraction from three controls and three AD cases. Ponceau S stain and 2,2,7-trimethylguanosine cap labeling is shown. B–F . Quantitative real-time PCR for U1, U2, U4, U5 and U6 using cDNA reverse transcribed from control and AD insoluble fraction RNA. Average bar is show in each panel. 2^(-Delta delta Ct) values are shown. n = 4. *Statistical significance ( P = 0.003, Student’s t -test).

    Article Snippet: TaqMan primers ( ) for U1, U2, U4, U5 and U6 along with 250 ng of cDNA template were used to perform quantitative real-time PCR (Applied Biosystems 7500 Fast Real-Time PCR System, Foster City, CA, USA).

    Techniques: Dot Blot, Staining, Labeling, Real-time Polymerase Chain Reaction

    Activation of STAT1 and STAT3 by U1-snRNA. ( A and B ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After the indicated time periods, cellular content of pIRF3 (A and B), pSTAT1 (A) and pSTAT3 (B) was determined by immunoblot analysis. For detection of pIRF3 and pSTAT1/3 on the same blot, blots were cut in half. For each experimental setup, one representative of three independently performed experiments is shown. ( C ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After 8 h, cellular IL-18BP mRNA expression was assessed by quantitative realtime PCR analysis. IL-18BP mRNA was normalized to that of GAPDH and is shown as fold induction compared with mock-stimulated control ± SD ( n = 3); ** P

    Journal: Nucleic Acids Research

    Article Title: Activation of interferon regulatory factor-3 via toll-like receptor 3 and immunomodulatory functions detected in A549 lung epithelial cells exposed to misplaced U1-snRNA

    doi: 10.1093/nar/gkp525

    Figure Lengend Snippet: Activation of STAT1 and STAT3 by U1-snRNA. ( A and B ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After the indicated time periods, cellular content of pIRF3 (A and B), pSTAT1 (A) and pSTAT3 (B) was determined by immunoblot analysis. For detection of pIRF3 and pSTAT1/3 on the same blot, blots were cut in half. For each experimental setup, one representative of three independently performed experiments is shown. ( C ) A549 cells were either kept as mock-transfected control or stimulated with U1-snRNA (0.1 µg/ml) or U1 ctr (0.003 µg/ml). After 8 h, cellular IL-18BP mRNA expression was assessed by quantitative realtime PCR analysis. IL-18BP mRNA was normalized to that of GAPDH and is shown as fold induction compared with mock-stimulated control ± SD ( n = 3); ** P

    Article Snippet: Quantitative realtime PCR of cDNA was performed on 7500 FAST Realtime PCR System (Applied Biosystems): One initial step at 95°C for 20 s was followed by 40 cycles at 95°C for 3 s and 60°C for 30 s. Detection, calculation of threshold cycles (C t values), and data analysis was performed by Sequence Detector software. mRNA was quantified by use of cloned cDNA standards for IL-18BP and GAPDH, respectively.

    Techniques: Activation Assay, Transfection, Expressing, Polymerase Chain Reaction

    Chemical structure and DNA binding properties of PM01183. (A) Structure of PM01183; (B) binding to naked DNA. Drugs were incubated with a 250 bp PCR product at 25°C during 1 h and the electrophoresis was run in 2% agarose-TAE; (C) relative binding affinities for different DNA triplet sequences. PM01183 was incubated with the labelled double-strand oligodeoxynucleotides at 25°C during 1 h; then the melting assay was started in a 7500 Fast Real-Time PCR System by increasing the temperature up to 95°C in small steps of 1°C·min −1 . Analysis of the 1/C 50 parameter (drug affinity for specific DNA sequences) and ΔT m (max) (stability of the drug–DNA complex) were analysed with an in-house developed Visual Basic Application (VBA) running on Microsoft Excel. The values correspond to the mean and standard deviation from at least two independent experiments for each sequence.

    Journal: British Journal of Pharmacology

    Article Title: PM01183, a new DNA minor groove covalent binder with potent in vitro and in vivo anti-tumour activity

    doi: 10.1111/j.1476-5381.2010.00945.x

    Figure Lengend Snippet: Chemical structure and DNA binding properties of PM01183. (A) Structure of PM01183; (B) binding to naked DNA. Drugs were incubated with a 250 bp PCR product at 25°C during 1 h and the electrophoresis was run in 2% agarose-TAE; (C) relative binding affinities for different DNA triplet sequences. PM01183 was incubated with the labelled double-strand oligodeoxynucleotides at 25°C during 1 h; then the melting assay was started in a 7500 Fast Real-Time PCR System by increasing the temperature up to 95°C in small steps of 1°C·min −1 . Analysis of the 1/C 50 parameter (drug affinity for specific DNA sequences) and ΔT m (max) (stability of the drug–DNA complex) were analysed with an in-house developed Visual Basic Application (VBA) running on Microsoft Excel. The values correspond to the mean and standard deviation from at least two independent experiments for each sequence.

    Article Snippet: For the experiments, we followed the methodology previously described ( ; ) using a 7500 Fast Real-Time PCR System (ABI Prism, Applied Biosystems, Foster City, CA, USA).

    Techniques: Binding Assay, Incubation, Polymerase Chain Reaction, Electrophoresis, Real-time Polymerase Chain Reaction, Standard Deviation, Sequencing