7300 real time pcr system Search Results


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  • 99
    Thermo Fisher steponeplus real time pcr system
    Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer 7300 real time pcr
    7300 Real Time Pcr, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time pcr system
    Attenuated T H 2 cytokines and chemokines in Mmp7 −/− mice Mmp7 −/− mice and age and sex matched wild-type (n=5 per group) mice immunized as described in Fig. 2 and 24 h after the last immunization, concentrations of (a) IL-4, (b) IL-5 and (c) IL-13 were measured in BAL fluid using Luminex. (d) Level of <t>CCL11</t> in BAL fluid was measured by ELISA. (e) Expression of Il-25 mRNA in the lung of WT and Mmp7 −/− CAA immunized mice was measured by real time <t>RT-PCR.</t> Data was normalized to actin. (Data represent mean values ± SD; representative of 3 independent experiments). * P
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7300 real time pcr
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7500 real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad 7300 real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7300 real time pcr machine
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Azco Biotech 7300 real time pcr thermocycler
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr Thermocycler, supplied by Azco Biotech, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer 7300 real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene 7300 real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr System, supplied by Stratagene, used in various techniques. Bioz Stars score: 87/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche 7300 real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    7300 Real Time Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi 7300 real time pcr
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    Abi 7300 Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher viia real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    Viia Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fast real time pcr system
    Results of <t>hybrid-PCR</t> . (A) Hybrid-PCR was carried out as described. Product of hybrid-PCR (PmiR-UL112-1) and mRNA-derived cDNA (cDNA) were electrophoresis on 3% agarose gel with DL2000 alongside. (B) Partial chromatogram of clone B29, which was identified containing HCMV IE72 specific sequence. Sequence of <t>miR-UL112-1</t> hybrid-primer was indicated in red box, and inner primer binding site was indicated in green box. PolyA sequence was down lined in black.
    Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time pcr model 7300
    Results of <t>hybrid-PCR</t> . (A) Hybrid-PCR was carried out as described. Product of hybrid-PCR (PmiR-UL112-1) and mRNA-derived cDNA (cDNA) were electrophoresis on 3% agarose gel with DL2000 alongside. (B) Partial chromatogram of clone B29, which was identified containing HCMV IE72 specific sequence. Sequence of <t>miR-UL112-1</t> hybrid-primer was indicated in red box, and inner primer binding site was indicated in green box. PolyA sequence was down lined in black.
    Real Time Pcr Model 7300, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time pcr
    Expression of IL-22 and IL-22R during septic peritonitis. (A and B) Organs were removed from wild-type C57BL/6 mice before (0 h) and 3 h, 6 h, and 12 h after CASP. Organs were homogenized, and RNA was prepared. IL-22, IL-22R1, IL-10, <t>IFN-γ,</t> IL-10R2, IL-10R2, and IL-22BP levels were determined by quantitative <t>PCR.</t> Expression levels are given relative to those before CASP as calibrator. Results are derived from four mice per group and time point. (C) Constitutive expression of IL-22BP mRNA was detected by reverse transcription-PCR in spleen 0 h and 3 h, respectively, after sepsis induction in three individual mice. (D) Spleens were removed from wild-type C57BL/6 mice before (0 h) and 6 h after CASP. T, B, and non-T/B cells were isolated by MACS and flow cytometry cell sorting, and RNA was prepared. IL-22 levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four individual experiments. (E) Purity of B220- (left panel) or Thy1.2-positive (right panel) cell populations used for IL-22 quantitative PCR determinations after magnetic sorting. *, P
    Quantitative Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 32963 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht real time pcr system
    Expression of IL-22 and IL-22R during septic peritonitis. (A and B) Organs were removed from wild-type C57BL/6 mice before (0 h) and 3 h, 6 h, and 12 h after CASP. Organs were homogenized, and RNA was prepared. IL-22, IL-22R1, IL-10, <t>IFN-γ,</t> IL-10R2, IL-10R2, and IL-22BP levels were determined by quantitative <t>PCR.</t> Expression levels are given relative to those before CASP as calibrator. Results are derived from four mice per group and time point. (C) Constitutive expression of IL-22BP mRNA was detected by reverse transcription-PCR in spleen 0 h and 3 h, respectively, after sepsis induction in three individual mice. (D) Spleens were removed from wild-type C57BL/6 mice before (0 h) and 6 h after CASP. T, B, and non-T/B cells were isolated by MACS and flow cytometry cell sorting, and RNA was prepared. IL-22 levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four individual experiments. (E) Purity of B220- (left panel) or Thy1.2-positive (right panel) cell populations used for IL-22 quantitative PCR determinations after magnetic sorting. *, P
    7900ht Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6868 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantstudio 5 real time pcr system
    Expression of IL-22 and IL-22R during septic peritonitis. (A and B) Organs were removed from wild-type C57BL/6 mice before (0 h) and 3 h, 6 h, and 12 h after CASP. Organs were homogenized, and RNA was prepared. IL-22, IL-22R1, IL-10, <t>IFN-γ,</t> IL-10R2, IL-10R2, and IL-22BP levels were determined by quantitative <t>PCR.</t> Expression levels are given relative to those before CASP as calibrator. Results are derived from four mice per group and time point. (C) Constitutive expression of IL-22BP mRNA was detected by reverse transcription-PCR in spleen 0 h and 3 h, respectively, after sepsis induction in three individual mice. (D) Spleens were removed from wild-type C57BL/6 mice before (0 h) and 6 h after CASP. T, B, and non-T/B cells were isolated by MACS and flow cytometry cell sorting, and RNA was prepared. IL-22 levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four individual experiments. (E) Purity of B220- (left panel) or Thy1.2-positive (right panel) cell populations used for IL-22 quantitative PCR determinations after magnetic sorting. *, P
    Quantstudio 5 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1017 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time pcr machine
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time pcr thermocycler
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Real Time Pcr Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time abi 7300 fast real time pcr system
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Real Time Abi 7300 Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time pcr apparatus
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Real Time Pcr Apparatus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher model 7300 real time pcr system
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Model 7300 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi 7300 real time pcr machine
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Abi 7300 Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi 7300 real time pcr thermocycler
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Abi 7300 Real Time Pcr Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi prism 7300 real time pcr
    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
    Abi Prism 7300 Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Real-time polymerase chain reaction <t>(PCR)</t> and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, <t>ALP,</t> Col I, and OPN in hAECs
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    Image Search Results


    Attenuated T H 2 cytokines and chemokines in Mmp7 −/− mice Mmp7 −/− mice and age and sex matched wild-type (n=5 per group) mice immunized as described in Fig. 2 and 24 h after the last immunization, concentrations of (a) IL-4, (b) IL-5 and (c) IL-13 were measured in BAL fluid using Luminex. (d) Level of CCL11 in BAL fluid was measured by ELISA. (e) Expression of Il-25 mRNA in the lung of WT and Mmp7 −/− CAA immunized mice was measured by real time RT-PCR. Data was normalized to actin. (Data represent mean values ± SD; representative of 3 independent experiments). * P

    Journal: Nature immunology

    Article Title: Divergent Roles for Airway Epithelial MMP7 and Retinoic Acid in Experimental Asthma

    doi: 10.1038/ni.1719

    Figure Lengend Snippet: Attenuated T H 2 cytokines and chemokines in Mmp7 −/− mice Mmp7 −/− mice and age and sex matched wild-type (n=5 per group) mice immunized as described in Fig. 2 and 24 h after the last immunization, concentrations of (a) IL-4, (b) IL-5 and (c) IL-13 were measured in BAL fluid using Luminex. (d) Level of CCL11 in BAL fluid was measured by ELISA. (e) Expression of Il-25 mRNA in the lung of WT and Mmp7 −/− CAA immunized mice was measured by real time RT-PCR. Data was normalized to actin. (Data represent mean values ± SD; representative of 3 independent experiments). * P

    Article Snippet: One step real-time quantitative RT-PCR was used to determine the relative expression of Ccl11 (eotaxin-1) and Raldh-1 in Real-Time PCR System (7300 Applied Biosystems).

    Techniques: Mouse Assay, Luminex, Enzyme-linked Immunosorbent Assay, Expressing, Cellular Antioxidant Activity Assay, Quantitative RT-PCR

    H2A.B regulates the transcription of the imprinted Igf2r locus. ( A ) Schematic sketch of the Igf2r locus. ( B ) Retinoic acid (RA) induces the enrichment of H2A.B at the Igf2r locus. ChIP analysis on the Igf2r locus is performed in mouse ES cells with or without RA treatment. Data are presented as mean ± SEM ( n = 3). ( C , D ) H2A.B is deposited at the maternally methylated DMR of Igf2r following RA treatment. Allele-specific incorporation of H2A.B was determined by DNA sequencing ( C ) or PCR-SSCP ( D ) (see Methods). ( E ) Knockdown of H2A.B suppresses the incorporation of H2A.B at the DMR of Igf2r . ChIP analysis is performed in shRNA-treated ES cells with or without RA induction. Data are presented as mean ± SEM ( n = 3). ( F ) Knockdown of H2A.B suppresses the RA-induced transcription of Igf2r . The relative mRNA level of Igf2r is examined by qPCR. Data are presented as mean ± SEM ( n = 4). ( G ) Knockdown of H2A.B suppresses the transcription of Igf2r from the maternal allele following RA treatment. Left panel shows that maternal and paternal alleles are digested by Taq I into different patterns because of SNP. Right panel shows that the transcription of Igf2r is dependent on the maternally methylated allele following RA treatment, and that depletion of H2A.B suppresses the RA-induced transcription of Igf2r from the maternal allele. The reduction of the transcription of Igf2r from each allele following RA treatment is summarized in the histogram.

    Journal: Genome Research

    Article Title: H2A.B facilitates transcription elongation at methylated CpG loci

    doi: 10.1101/gr.156877.113

    Figure Lengend Snippet: H2A.B regulates the transcription of the imprinted Igf2r locus. ( A ) Schematic sketch of the Igf2r locus. ( B ) Retinoic acid (RA) induces the enrichment of H2A.B at the Igf2r locus. ChIP analysis on the Igf2r locus is performed in mouse ES cells with or without RA treatment. Data are presented as mean ± SEM ( n = 3). ( C , D ) H2A.B is deposited at the maternally methylated DMR of Igf2r following RA treatment. Allele-specific incorporation of H2A.B was determined by DNA sequencing ( C ) or PCR-SSCP ( D ) (see Methods). ( E ) Knockdown of H2A.B suppresses the incorporation of H2A.B at the DMR of Igf2r . ChIP analysis is performed in shRNA-treated ES cells with or without RA induction. Data are presented as mean ± SEM ( n = 3). ( F ) Knockdown of H2A.B suppresses the RA-induced transcription of Igf2r . The relative mRNA level of Igf2r is examined by qPCR. Data are presented as mean ± SEM ( n = 4). ( G ) Knockdown of H2A.B suppresses the transcription of Igf2r from the maternal allele following RA treatment. Left panel shows that maternal and paternal alleles are digested by Taq I into different patterns because of SNP. Right panel shows that the transcription of Igf2r is dependent on the maternally methylated allele following RA treatment, and that depletion of H2A.B suppresses the RA-induced transcription of Igf2r from the maternal allele. The reduction of the transcription of Igf2r from each allele following RA treatment is summarized in the histogram.

    Article Snippet: Quantitative-PCR (qPCR) was performed using Power SYBR green PCR master mix in 7300 real-time PCR systems (Applied Biosystems).

    Techniques: Chromatin Immunoprecipitation, Methylation, DNA Sequencing, Polymerase Chain Reaction, shRNA, Real-time Polymerase Chain Reaction

    Real-time quantitative RT-PCR analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).

    Journal: The Journal of parasitology

    Article Title: DIFFERENCES IN CYSTEINE PROTEASE ACTIVITY IN SCHISTOSOMA MANSONI-RESISTANT AND -SUSCEPTIBLE BIOMPHALARIA GLABRATA AND CHARACTERIZATION OF THE HEPATOPANCREAS CATHEPSIN B FULL-LENGTH cDNA

    doi: 10.1645/GE-1410R.1

    Figure Lengend Snippet: Real-time quantitative RT-PCR analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).

    Article Snippet: The RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System (Applied Biosystems, Foster City, California).

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Labeling, Expressing

    Effect of SP on EfTu mRNA transcription in B. cereu s. Expression of EfTu mRNAs was quantified by qRT-PCR using primers presented in Table 1 and a 7300 Real Time PCR System apparatus. EfTu expression was normalized using 16S mRNA as reference. ★★★ p

    Journal: Scientific Reports

    Article Title: Mechanism of action of the moonlighting protein EfTu as a Substance P sensor in Bacillus cereus

    doi: 10.1038/s41598-018-37506-6

    Figure Lengend Snippet: Effect of SP on EfTu mRNA transcription in B. cereu s. Expression of EfTu mRNAs was quantified by qRT-PCR using primers presented in Table 1 and a 7300 Real Time PCR System apparatus. EfTu expression was normalized using 16S mRNA as reference. ★★★ p

    Article Snippet: PCR reactions were performed in triplicate with the 7300 Real Time PCR System apparatus (Applied Biosystems, Illkirch, France).

    Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    CypA Kd-mediated down-regulation of IL-8, IL-6, and IL-1β expression. IL-8, IL-6, and IL-1β expression levels were determined by a quantitative real-time PCR (qRT-PCR) analysis. One microgram of total RNA was used for cDNA synthesis with Superscript III (Invitrogen) and the oligo(dT)15 primer. qRT-PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) detected by the 7300 Real Time PCR System (Applied Biosystems). The relative expression levels of IL-8, IL-6, and IL-1β mRNA were normalized to the internal reference 18S rRNA.

    Journal: PLoS ONE

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling

    doi: 10.1371/journal.pone.0096211

    Figure Lengend Snippet: CypA Kd-mediated down-regulation of IL-8, IL-6, and IL-1β expression. IL-8, IL-6, and IL-1β expression levels were determined by a quantitative real-time PCR (qRT-PCR) analysis. One microgram of total RNA was used for cDNA synthesis with Superscript III (Invitrogen) and the oligo(dT)15 primer. qRT-PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) detected by the 7300 Real Time PCR System (Applied Biosystems). The relative expression levels of IL-8, IL-6, and IL-1β mRNA were normalized to the internal reference 18S rRNA.

    Article Snippet: The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, SYBR Green Assay, Polymerase Chain Reaction

    SG-PERT assay on ABI 7300 Real-Time PCR System. (A) Amplification curves of indicated amount of replication competent HIV-1 (NL4-3 strain) (ng p24/mL) obtained by SG-PERT on the ABI 7300 instrument. The horizontal line represents the threshold line used to calculate Cq values. (B) Correlation between input levels of replication-competent HIV-1 virus and Cq values obtained by SG-PERT on the ABI 7300 instrument. p24 antigen concentration in the undiluted samples (value “0” on x-axis) was 3,100 ng/mL.

    Journal: PLoS ONE

    Article Title: Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

    doi: 10.1371/journal.pone.0050859

    Figure Lengend Snippet: SG-PERT assay on ABI 7300 Real-Time PCR System. (A) Amplification curves of indicated amount of replication competent HIV-1 (NL4-3 strain) (ng p24/mL) obtained by SG-PERT on the ABI 7300 instrument. The horizontal line represents the threshold line used to calculate Cq values. (B) Correlation between input levels of replication-competent HIV-1 virus and Cq values obtained by SG-PERT on the ABI 7300 instrument. p24 antigen concentration in the undiluted samples (value “0” on x-axis) was 3,100 ng/mL.

    Article Snippet: For SG-PERT on the ABI 7300 real-time PCR system (Applied Biosystems, Foster City, CA, USA), a reaction mix was made using the ROX-containing qPCR Core kit for SYBR Green I from Eurogentec (Catalog #RT-QP73-05).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Concentration Assay

    Effect of bone morphogenic protein 2 ( BMP - 2 ) and 5-AzadC treatment on SOST expression. a and b Efects of BMP-2 on SOST mRNA expression levels in cultured normal (n = 3) and osteoarthritis ( OA ) chondrocytes (n = 3). Osteocalcin and LRP-5 used as positive controls that upregulated by BMP-2 and LRP-6 and MMP-7 used as negative controls that not regulated by BMP-2( error bars = standard error, * p = 0.004). c Detection of SOST mRNA expression levels by real time PCR after BMP-2 treatment in cultured normal chondrocytes (n = 3) with or without 5-AzadC. GAPDH was used for normalization of the real-time PCR data ( error bars = standard error, * p = 0.001 versus control and DMSO/BMP-2 treatment). MMP matrix metalloproteinase, LRP low-density lipoprotein receptor-related protein

    Journal: Arthritis Research & Therapy

    Article Title: DNA methylation regulates sclerostin (SOST) expression in osteoarthritic chondrocytes by bone morphogenetic protein 2 (BMP-2) induced changes in Smads binding affinity to the CpG region of SOST promoter

    doi: 10.1186/s13075-015-0674-6

    Figure Lengend Snippet: Effect of bone morphogenic protein 2 ( BMP - 2 ) and 5-AzadC treatment on SOST expression. a and b Efects of BMP-2 on SOST mRNA expression levels in cultured normal (n = 3) and osteoarthritis ( OA ) chondrocytes (n = 3). Osteocalcin and LRP-5 used as positive controls that upregulated by BMP-2 and LRP-6 and MMP-7 used as negative controls that not regulated by BMP-2( error bars = standard error, * p = 0.004). c Detection of SOST mRNA expression levels by real time PCR after BMP-2 treatment in cultured normal chondrocytes (n = 3) with or without 5-AzadC. GAPDH was used for normalization of the real-time PCR data ( error bars = standard error, * p = 0.001 versus control and DMSO/BMP-2 treatment). MMP matrix metalloproteinase, LRP low-density lipoprotein receptor-related protein

    Article Snippet: DNA methylation analysis by qMSP Quantitative methylation-specific PCR (qMSP) for the CpG island of the SOST promoter was performed using a real-time PCR instrument (ABI 7300, Applied Biosystems, Foster, CA, USA).

    Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction

    Effect of 5-AzadC treatment on SOST expression and DNA methylation status in the CpG-rich region of the SOST promoter. a Quantitative SOST mRNA expression in cultured normal chondrocytes (n = 3) after treatment with 5 μM 5-AzadC. GAPDH was used for normalization of the real-time PCR data ( error bars = standard error, * p = 0.041). b Representative western blot of SOST protein levels in cultured normal chondrocytes after treatment with 5 μM 5-AzadC and a bar graph showing relative SOST protein expression normalized to β-actin in 5-AzadC-treated normal chondrocytes (n = 3) ( error bars = standard error, * p = 0.009). c DNA methylation and unmethylation status of the SOST promoter in cultured normal chondrocytes after treatment with 5 μM 5-AzadC. d DNA methylation values in CpG-rich region of the SOST promoter in cultured normal chondrocytes (n = 3) after treatment with 5 μM 5-AzadC by quantitative methylation-specific PCR ( error bars = standard error, * p = 0.032)

    Journal: Arthritis Research & Therapy

    Article Title: DNA methylation regulates sclerostin (SOST) expression in osteoarthritic chondrocytes by bone morphogenetic protein 2 (BMP-2) induced changes in Smads binding affinity to the CpG region of SOST promoter

    doi: 10.1186/s13075-015-0674-6

    Figure Lengend Snippet: Effect of 5-AzadC treatment on SOST expression and DNA methylation status in the CpG-rich region of the SOST promoter. a Quantitative SOST mRNA expression in cultured normal chondrocytes (n = 3) after treatment with 5 μM 5-AzadC. GAPDH was used for normalization of the real-time PCR data ( error bars = standard error, * p = 0.041). b Representative western blot of SOST protein levels in cultured normal chondrocytes after treatment with 5 μM 5-AzadC and a bar graph showing relative SOST protein expression normalized to β-actin in 5-AzadC-treated normal chondrocytes (n = 3) ( error bars = standard error, * p = 0.009). c DNA methylation and unmethylation status of the SOST promoter in cultured normal chondrocytes after treatment with 5 μM 5-AzadC. d DNA methylation values in CpG-rich region of the SOST promoter in cultured normal chondrocytes (n = 3) after treatment with 5 μM 5-AzadC by quantitative methylation-specific PCR ( error bars = standard error, * p = 0.032)

    Article Snippet: DNA methylation analysis by qMSP Quantitative methylation-specific PCR (qMSP) for the CpG island of the SOST promoter was performed using a real-time PCR instrument (ABI 7300, Applied Biosystems, Foster, CA, USA).

    Techniques: Expressing, DNA Methylation Assay, Cell Culture, Real-time Polymerase Chain Reaction, Western Blot, Methylation, Polymerase Chain Reaction

    SOST mRNA and protein expression levels in normal and osteoarthritis ( OA ) chondrocytes. a Quantitative SOST mRNA expression in cultured normal (n = 10) and OA chondrocytes (n = 14). GAPDH was used for normalization of the real-time PCR data ( error bars = standard error, * p = 0.005). b Representative western blot of SOST protein expression in cultured normal and OA chondrocytes and a bar graph showing relative SOST protein expression normalized to β-actin in normal (n = 5) and OA chondrocytes (n = 5) ( error bars = standard error, * p = 0.038)

    Journal: Arthritis Research & Therapy

    Article Title: DNA methylation regulates sclerostin (SOST) expression in osteoarthritic chondrocytes by bone morphogenetic protein 2 (BMP-2) induced changes in Smads binding affinity to the CpG region of SOST promoter

    doi: 10.1186/s13075-015-0674-6

    Figure Lengend Snippet: SOST mRNA and protein expression levels in normal and osteoarthritis ( OA ) chondrocytes. a Quantitative SOST mRNA expression in cultured normal (n = 10) and OA chondrocytes (n = 14). GAPDH was used for normalization of the real-time PCR data ( error bars = standard error, * p = 0.005). b Representative western blot of SOST protein expression in cultured normal and OA chondrocytes and a bar graph showing relative SOST protein expression normalized to β-actin in normal (n = 5) and OA chondrocytes (n = 5) ( error bars = standard error, * p = 0.038)

    Article Snippet: DNA methylation analysis by qMSP Quantitative methylation-specific PCR (qMSP) for the CpG island of the SOST promoter was performed using a real-time PCR instrument (ABI 7300, Applied Biosystems, Foster, CA, USA).

    Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Western Blot

    Results of hybrid-PCR . (A) Hybrid-PCR was carried out as described. Product of hybrid-PCR (PmiR-UL112-1) and mRNA-derived cDNA (cDNA) were electrophoresis on 3% agarose gel with DL2000 alongside. (B) Partial chromatogram of clone B29, which was identified containing HCMV IE72 specific sequence. Sequence of miR-UL112-1 hybrid-primer was indicated in red box, and inner primer binding site was indicated in green box. PolyA sequence was down lined in black.

    Journal: Virology Journal

    Article Title: A rapid method to screen putative mRNA targets of any known microRNA

    doi: 10.1186/1743-422X-8-8

    Figure Lengend Snippet: Results of hybrid-PCR . (A) Hybrid-PCR was carried out as described. Product of hybrid-PCR (PmiR-UL112-1) and mRNA-derived cDNA (cDNA) were electrophoresis on 3% agarose gel with DL2000 alongside. (B) Partial chromatogram of clone B29, which was identified containing HCMV IE72 specific sequence. Sequence of miR-UL112-1 hybrid-primer was indicated in red box, and inner primer binding site was indicated in green box. PolyA sequence was down lined in black.

    Article Snippet: Expression of mature miR-UL112-1 was measured by TaqMan® microRNA assays on 7300 Fast Real-Time PCR System (Applied Biosystems) (data not shown).

    Techniques: Polymerase Chain Reaction, Derivative Assay, Electrophoresis, Agarose Gel Electrophoresis, Sequencing, Binding Assay

    Protocol of hybrid-PCR . (A) Schematic presentation of principle and process designed for hybrid-PCR. (B) Diagram showing sequences of miR-UL112-1 and miR-UL112-1 hybrid primer. Positions marked by Red R meant random insertions of A or G. Seed region was indicated by green box surrounding nucleotide 2-7 of miR-UL112-1.

    Journal: Virology Journal

    Article Title: A rapid method to screen putative mRNA targets of any known microRNA

    doi: 10.1186/1743-422X-8-8

    Figure Lengend Snippet: Protocol of hybrid-PCR . (A) Schematic presentation of principle and process designed for hybrid-PCR. (B) Diagram showing sequences of miR-UL112-1 and miR-UL112-1 hybrid primer. Positions marked by Red R meant random insertions of A or G. Seed region was indicated by green box surrounding nucleotide 2-7 of miR-UL112-1.

    Article Snippet: Expression of mature miR-UL112-1 was measured by TaqMan® microRNA assays on 7300 Fast Real-Time PCR System (Applied Biosystems) (data not shown).

    Techniques: Polymerase Chain Reaction

    Expression of IL-22 and IL-22R during septic peritonitis. (A and B) Organs were removed from wild-type C57BL/6 mice before (0 h) and 3 h, 6 h, and 12 h after CASP. Organs were homogenized, and RNA was prepared. IL-22, IL-22R1, IL-10, IFN-γ, IL-10R2, IL-10R2, and IL-22BP levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four mice per group and time point. (C) Constitutive expression of IL-22BP mRNA was detected by reverse transcription-PCR in spleen 0 h and 3 h, respectively, after sepsis induction in three individual mice. (D) Spleens were removed from wild-type C57BL/6 mice before (0 h) and 6 h after CASP. T, B, and non-T/B cells were isolated by MACS and flow cytometry cell sorting, and RNA was prepared. IL-22 levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four individual experiments. (E) Purity of B220- (left panel) or Thy1.2-positive (right panel) cell populations used for IL-22 quantitative PCR determinations after magnetic sorting. *, P

    Journal: Infection and Immunity

    Article Title: Inhibition of Interleukin-22 Attenuates Bacterial Load and Organ Failure during Acute Polymicrobial Sepsis ▿

    doi: 10.1128/IAI.01564-06

    Figure Lengend Snippet: Expression of IL-22 and IL-22R during septic peritonitis. (A and B) Organs were removed from wild-type C57BL/6 mice before (0 h) and 3 h, 6 h, and 12 h after CASP. Organs were homogenized, and RNA was prepared. IL-22, IL-22R1, IL-10, IFN-γ, IL-10R2, IL-10R2, and IL-22BP levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four mice per group and time point. (C) Constitutive expression of IL-22BP mRNA was detected by reverse transcription-PCR in spleen 0 h and 3 h, respectively, after sepsis induction in three individual mice. (D) Spleens were removed from wild-type C57BL/6 mice before (0 h) and 6 h after CASP. T, B, and non-T/B cells were isolated by MACS and flow cytometry cell sorting, and RNA was prepared. IL-22 levels were determined by quantitative PCR. Expression levels are given relative to those before CASP as calibrator. Results are derived from four individual experiments. (E) Purity of B220- (left panel) or Thy1.2-positive (right panel) cell populations used for IL-22 quantitative PCR determinations after magnetic sorting. *, P

    Article Snippet: The expression of IL-22, IL-10, gamma interferon (IFN-γ), IL-22R1, IL-10R2, IL-10R2, and IL-22BP was analyzed using quantitative real-time PCR (ABI 7300 Real Time PCR System; Applied Biosystems, Foster City, CA) in liver, kidney, and spleen RNA samples from mice 0 h, 3 h, 6 h, and 12 h after sepsis induction.

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Derivative Assay, Polymerase Chain Reaction, Isolation, Magnetic Cell Separation, Flow Cytometry, Cytometry, FACS

    Real-time polymerase chain reaction (PCR) and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, ALP, Col I, and OPN in hAECs

    Journal: Stem Cells Translational Medicine

    Article Title: Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration

    doi: 10.5966/sctm.2014-0118

    Figure Lengend Snippet: Real-time polymerase chain reaction (PCR) and immunofluorescence study for osteogenic differentiation of hAECs in vitro. (A): Real-time PCR assay of osteoblastic marker genes showed significant upregulation of Runx2, Osx, ALP, Col I, and OPN in hAECs

    Article Snippet: To analyze the expression of specific genes for osteoblastic differentiation, the expression of Runx2, osterix, ALP, collagen I, and osteopontin (OPN) was determined quantitatively on a real-time PCR machine (ABI 7300; Applied Biosystems, Foster City, CA, ) using a SYBR Premix Ex Taq kit (Takara), with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the reference gene for normalization.

    Techniques: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Immunofluorescence, In Vitro, Marker, ALP Assay