Journal:

Article Title: Type 3 Fimbrial Shaft (MrkA) of Klebsiella pneumoniae , but Not the Fimbrial Adhesin (MrkD), Facilitates Biofilm Formation

doi: 10.1128/IAI.69.9.5805-5812.2001

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: The conditions for transformation and characterization of plasmids in strains of K. pneumoniae have been described elsewhere ( 13 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Genotype and/or relevant feature Reference or source K. pneumoniae strains IA565 Wild type; complete mrk gene cluster possessing collagen-binding MrkD 1P adhesin 27 IApc35 Fim + Adh − derivative of IA565 lacking the plasmid-borne mrkD 1P gene 27 IAΔT3 mrkB ::Tn 5 ; Fim − Adh − derivative of IApc35 possessing a mutation in the fimbrial chaperone 30 43816 Type 3 fimbriate strain with mrkD 1C2 allele ATCC; 27 MBM100 Nonfimbriate Tn 5 mutant of 43816 This study Plasmids pFK12 Complete mrk gene cluster possessing the mrkD 1P allele 2 pUT Plasmid possessing the Mini-Tn 5Km2 element 8 pBBRMCS-1 GFP expressing, Cm resistant 24 Open in a separate window Strains and plasmids used in this study

Techniques: Plasmid Preparation, Mutagenesis, Expressing