Journal: Nutrients

Article Title: Metabolic Trajectories Following Contrasting Prudent and Western Diets from Food Provisions: Identifying Robust Biomarkers of Short-Term Changes in Habitual Diet

doi: 10.3390/nu11102407

Figure Lengend Snippet: Top-ranked plasma metabolites associated with contrasting diets by DIGEST participants ( n = 42) when using time series MEBA, mixed ANOVA, and partial correlation analysis.

Article Snippet: Proline betaine was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada).

Techniques:

Journal: Nutrients

Article Title: Metabolic Trajectories Following Contrasting Prudent and Western Diets from Food Provisions: Identifying Robust Biomarkers of Short-Term Changes in Habitual Diet

doi: 10.3390/nu11102407

Figure Lengend Snippet: Top-ranked creatinine-normalized metabolites associated with contrasting diets by DIGEST participants ( n = 42) when using time series MEBA, mixed ANOVA, and partial correlation analysis.

Article Snippet: Proline betaine was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada).

Techniques:

Journal: Scientific Reports

Article Title: The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation

doi: 10.1038/s41598-019-46279-5

Figure Lengend Snippet: Recombinant RMND5A and MAEA exhibit E3 ligase activity in vitro . ( a ) Bacterially expressed RMND5A exhibits E3 ligase activity. Bacterial extracts with induced expression of GST (lane 2) or GST-RMND5A (lane 6) were incubated with ATP and His-ubiquitin and with or without, as indicated, His-E1 and UBE2D2 (E2) for 2 hours at 37 °C. Reactions were analyzed by Western blot using anti-His antibody (top), or an RMND5A antibody (bottom). The identity of the products is indicated on the right. ( b ) SUMO-MAEA has in vitro ligase activity. His-tagged SUMO MAEA was expressed and incubated in reaction as in. ( a ) His-tagged SUMO-SLP2 was used as a negative control. Analysis was done by Western blot analyzed using an anti-His antibody (top), or a MAEA antibody (bottom).

Article Snippet: 4.5 μL of bacterial extract was used in a 20 μL ubiquitination reaction with 5 μM UBE2D2 (E2-622, BostonBiochem, Cambridge, MA), 0.1 μM His-tagged E1 , 25 μM His-ubiquitin, 10 mM Mg-ATP (B-20, R&D Systems), 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM MgCl2, 0.1 mM DTT and 25 μM ZnCl 2 .

Techniques: Recombinant, Activity Assay, In Vitro, Expressing, Incubation, Western Blot, Negative Control