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  • 93
    Cell Signaling Technology Inc erk
    ANOVA results for Western blots.
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    ANOVA results for Western blots.
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    Arista Biologicals 7124 application switch
    ANOVA results for Western blots.
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    ANOVA results for Western blots.
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    Thermo Fisher 276 2009 7124 7133 ª 2009
    ANOVA results for Western blots.
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    Image Search Results


    ANOVA results for Western blots.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Negative Effects of Chronic Rapamycin Treatment on Behavior in a Mouse Model of Fragile X Syndrome

    doi: 10.3389/fnmol.2017.00452

    Figure Lengend Snippet: ANOVA results for Western blots.

    Article Snippet: Primary antibodies were used at a 1:1000 dilution and were as follows: FMRP (Abcam 27455), p-mTOR (Cell Signaling 5536), mTOR (Cell Signaling 2983), p-p70S6K (Cell Signaling 9234), p70S6K (Cell Signaling 2708), p-S6 235/236 (Cell Signaling 2211), p-S6 240/244 (Cell Signaling 2215), S6 (Cell Signaling 2217), p-ERK (Cell Signaling 4370), ERK (Cell Signaling 7124), p-AKT Ser473 (Cell Signaling 4060), and AKT (Cell Signaling 9272).

    Techniques: Western Blot

    Westerns blots of frontal cortex of Fmr1 KO and control mice on vehicle and rapamycin treatment. (A) Representative Western blot images. (B) p-mTOR levels did not differ among the groups. (C) mTOR levels did not differ among the groups. (D) p-mTOR/Total mTOR did not differ among the groups. (E) p-p70S6k did not differ among the groups. (F) p70S6k did not differ among the groups. (G) p-p70S6k/Total p70S6k did not differ among the groups. (H) The genotype × treatment interaction for pS6 235/236 was statistically significant. Post hoc t -tests revealed that vehicle-treated Fmr1 KO animals had significantly higher p-S6 235/236 ( p = 0.002) compared to vehicle-treated controls. This was significantly reduced by rapamycin treatment ( p = 0.002). (I) S6 levels did not differ among groups. (J) The genotype × treatment interaction for p-S6 (235/236)/Total S6 approached statistical significance. We looked at individual differences by means of post hoc t -tests and found that the difference between vehicle-treated controls and vehicle-treated Fmr1 KO mice was statistically significant ( p = 0.004). (K) The genotype × treatment interaction for p-S6 240/244 was statistically significant. ( Post hoc t -tests revealed that vehicle-treated Fmr1 KO animals had significantly higher p-S6 240/244 levels compared to vehicle-treated controls ( p = 0.010). This was significantly reduced with rapamycin treatment ( p = 0.006). (L) Total S6 levels did not differ among the groups. (M) The genotype × treatment interaction for p-S6 (240/244)/Total S6 approached statistical significance. We looked at individual differences by means of post hoc t -tests and found that the difference between vehicle-treated controls and vehicle-treated Fmr1 KO mice was statistically significant ( p = 0.016). (N) The genotype × treatment interaction for p-AKT Ser473 was statistically significant. Post hoc t -tests revealed that vehicle-treated Fmr1 KO animals had higher p-AKT compared to vehicle-treated controls ( p = 0.020). p-AKT Ser473 levels were reduced in Fmr1 KO animals after rapamycin treatment ( p = 0.013). (O) Total AKT levels did not differ among the groups. (P) p-Akt (473)/Akt did not differ among the groups. (Q) The main effect of treatment for p-ERK levels was statistically significant indicating that regardless of genotype, rapamycin reduced p-ERK. (R) The main effect of treatment for ERK levels was statistically significant indicating that regardless of genotype, rapamycin reduced ERK. (S) p-ERK/ERK did not differ among the groups. (B–S) Levels were normalized to total protein in the blot. Values presented are relative to the mean of vehicle-treated control values. Bars represent mean ± SEM. ∼0.05 < p ≤ 0.1, ∗ 0.01 ≤ p ≤ 0.05, ∗∗ 0.001 ≤ p ≤ 0.01 as determined by post hoc t -tests. n = 4 vehicle-treated control, n = 4 rapamycin-treated control, n = 3 vehicle-treated Fmr1 KO, n = 3 rapamycin-treated Fmr1 KO. )

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Negative Effects of Chronic Rapamycin Treatment on Behavior in a Mouse Model of Fragile X Syndrome

    doi: 10.3389/fnmol.2017.00452

    Figure Lengend Snippet: Westerns blots of frontal cortex of Fmr1 KO and control mice on vehicle and rapamycin treatment. (A) Representative Western blot images. (B) p-mTOR levels did not differ among the groups. (C) mTOR levels did not differ among the groups. (D) p-mTOR/Total mTOR did not differ among the groups. (E) p-p70S6k did not differ among the groups. (F) p70S6k did not differ among the groups. (G) p-p70S6k/Total p70S6k did not differ among the groups. (H) The genotype × treatment interaction for pS6 235/236 was statistically significant. Post hoc t -tests revealed that vehicle-treated Fmr1 KO animals had significantly higher p-S6 235/236 ( p = 0.002) compared to vehicle-treated controls. This was significantly reduced by rapamycin treatment ( p = 0.002). (I) S6 levels did not differ among groups. (J) The genotype × treatment interaction for p-S6 (235/236)/Total S6 approached statistical significance. We looked at individual differences by means of post hoc t -tests and found that the difference between vehicle-treated controls and vehicle-treated Fmr1 KO mice was statistically significant ( p = 0.004). (K) The genotype × treatment interaction for p-S6 240/244 was statistically significant. ( Post hoc t -tests revealed that vehicle-treated Fmr1 KO animals had significantly higher p-S6 240/244 levels compared to vehicle-treated controls ( p = 0.010). This was significantly reduced with rapamycin treatment ( p = 0.006). (L) Total S6 levels did not differ among the groups. (M) The genotype × treatment interaction for p-S6 (240/244)/Total S6 approached statistical significance. We looked at individual differences by means of post hoc t -tests and found that the difference between vehicle-treated controls and vehicle-treated Fmr1 KO mice was statistically significant ( p = 0.016). (N) The genotype × treatment interaction for p-AKT Ser473 was statistically significant. Post hoc t -tests revealed that vehicle-treated Fmr1 KO animals had higher p-AKT compared to vehicle-treated controls ( p = 0.020). p-AKT Ser473 levels were reduced in Fmr1 KO animals after rapamycin treatment ( p = 0.013). (O) Total AKT levels did not differ among the groups. (P) p-Akt (473)/Akt did not differ among the groups. (Q) The main effect of treatment for p-ERK levels was statistically significant indicating that regardless of genotype, rapamycin reduced p-ERK. (R) The main effect of treatment for ERK levels was statistically significant indicating that regardless of genotype, rapamycin reduced ERK. (S) p-ERK/ERK did not differ among the groups. (B–S) Levels were normalized to total protein in the blot. Values presented are relative to the mean of vehicle-treated control values. Bars represent mean ± SEM. ∼0.05 < p ≤ 0.1, ∗ 0.01 ≤ p ≤ 0.05, ∗∗ 0.001 ≤ p ≤ 0.01 as determined by post hoc t -tests. n = 4 vehicle-treated control, n = 4 rapamycin-treated control, n = 3 vehicle-treated Fmr1 KO, n = 3 rapamycin-treated Fmr1 KO. )

    Article Snippet: Primary antibodies were used at a 1:1000 dilution and were as follows: FMRP (Abcam 27455), p-mTOR (Cell Signaling 5536), mTOR (Cell Signaling 2983), p-p70S6K (Cell Signaling 9234), p70S6K (Cell Signaling 2708), p-S6 235/236 (Cell Signaling 2211), p-S6 240/244 (Cell Signaling 2215), S6 (Cell Signaling 2217), p-ERK (Cell Signaling 4370), ERK (Cell Signaling 7124), p-AKT Ser473 (Cell Signaling 4060), and AKT (Cell Signaling 9272).

    Techniques: Western Blot