7000 real time pcr system Search Results


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  • 99
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using <t>7000</t> Real-Time <t>PCR</t> System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p
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    Image Search Results


    The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using 7000 Real-Time PCR System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p

    Journal: Nutrients

    Article Title: Real Time Monitoring of Inhibition of Adipogenesis and Angiogenesis by (−)-Epigallocatechin-3-Gallate in 3T3-L1 Adipocytes and Human Umbilical Vein Endothelial Cells

    doi: 10.3390/nu7105437

    Figure Lengend Snippet: The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes ( A ) and adipocytes ( B ) as quantified using 7000 Real-Time PCR System (Applied Biosystems); ( C ) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); ( D ) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p

    Article Snippet: The reaction mixtures were heated at 25 °C for 5 min, 42 °C for 60 min and 70 °C for 15 min. Real-time PCR was performed using the 7000 Real-Time PCR System (Applied Biosystems, Foster, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation