66898 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Proteintech anti upf1
    siRNAs used in this study
    Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti upf1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti upf1 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Proteintech rabbit polyclonal anti upf1
    BC cell migration and proliferation are regulated by PVT1 and <t>UPF1.</t> A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
    Rabbit Polyclonal Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti upf1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti upf1 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Proteintech β actin primary antibody
    BC cell migration and proliferation are regulated by PVT1 and <t>UPF1.</t> A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
    β Actin Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin primary antibody/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β actin primary antibody - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Proteintech rabbit anti upf1
    (A) Representative images of Drosophila eyes expressing the noted <t>UPF1</t> expression modifiers in photoreceptors using a GMR driver. Top images are from flies expressing the noted alleles only, and bottom images are from flies expressing the noted alleles in the context of 30 GGGGCC repeats under the same driver. (B) Violin plots of eye degeneration scores of 30R flies expressing the noted UPF1 alleles. See details for details on scoring method. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. ****p < 0.0001. From left to right, n = 108, 54, 101, 15, 60, 15. Data are represented as violin plots with indicated quartiles. (C) Representative fields of view of propidium-iodide-positive iPSNs in the absence (top row) or presence (bottom row) of 10 μM glutamate. Scale bar, 100 μm. (D) Quantification of the relative proportion of propidium-iodide-positive (dead) cells to total cells from images in (C), represented as “% Cell Death.” n = 3 pairs of age- and sex-matched control and C9orf72 iPSNs, 2 replicates per pair. Six fields of view were analyzed for each data point. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. *p < 0.05, ****p < 0.0001. Data are indicated as mean ± SD. See also .
    Rabbit Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti upf1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti upf1 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    Proteintech upf1 primary antibody
    The tRF-Leu-AAG promoted PC development by suppressing <t>UPF1.</t>
    Upf1 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upf1 primary antibody/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    upf1 primary antibody - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    siRNAs used in this study

    Journal: Nucleic Acids Research

    Article Title: Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway

    doi: 10.1093/nar/gky1087

    Figure Lengend Snippet: siRNAs used in this study

    Article Snippet: Antibodies used for western blotting were as follows: anti-Flag (Sigma, F3165, Cell Signaling Technology, 2368), anti-Myc (Roche, 11667149001, Santa Cruz Biotechnology, sc-789), anti-Dom34 ( , raised against His-tagged Dom34(220–385)), anti-RNase L (raised against His-tagged RNase L(1–333)), anti-OAS3 (abcam, ab154270), anti-GAPDH , anti-Xrn1 (Bethyl Laboratories, A300–443A), anti-Ski2 (Proteintech, 11462–1-AP), anti-Mtr4 (Bethyl Laboratories, A300–614A) and anti-Upf1 (raised against a synthetic peptide corresponding to N-terminal residues of Upf1 (EEDEEDTYYTKDLPIHAC)).

    Techniques: Sequencing, Luciferase

    BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

    Journal: Breast Cancer Research : BCR

    Article Title: Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1

    doi: 10.1186/s13058-021-01491-y

    Figure Lengend Snippet: BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

    Article Snippet: The primary antibodies were: rabbit monoclonal anti-E-cadherin (#3195, 1:1000, Cell Signaling Technology), rabbit monoclonal anti-Vimentin (#5741, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-UPF1 (23379-1-AP, 1:1000, ProteinTech), rabbit polyclonal anti-FOXQ1 (23718-1-AP, 1:1000, ProteinTech), mouse monoclonal anti-β-actin (A5316, 1:5000, Sigma-Aldrich), and mouse monoclonal anti-PCNA (sc-25280, 1:1000, Santa Cruz).

    Techniques: Migration, Western Blot, Transfection

    (A) Representative images of Drosophila eyes expressing the noted UPF1 expression modifiers in photoreceptors using a GMR driver. Top images are from flies expressing the noted alleles only, and bottom images are from flies expressing the noted alleles in the context of 30 GGGGCC repeats under the same driver. (B) Violin plots of eye degeneration scores of 30R flies expressing the noted UPF1 alleles. See details for details on scoring method. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. ****p < 0.0001. From left to right, n = 108, 54, 101, 15, 60, 15. Data are represented as violin plots with indicated quartiles. (C) Representative fields of view of propidium-iodide-positive iPSNs in the absence (top row) or presence (bottom row) of 10 μM glutamate. Scale bar, 100 μm. (D) Quantification of the relative proportion of propidium-iodide-positive (dead) cells to total cells from images in (C), represented as “% Cell Death.” n = 3 pairs of age- and sex-matched control and C9orf72 iPSNs, 2 replicates per pair. Six fields of view were analyzed for each data point. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. *p < 0.05, ****p < 0.0001. Data are indicated as mean ± SD. See also .

    Journal: Cell reports

    Article Title: UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction

    doi: 10.1016/j.celrep.2021.108925

    Figure Lengend Snippet: (A) Representative images of Drosophila eyes expressing the noted UPF1 expression modifiers in photoreceptors using a GMR driver. Top images are from flies expressing the noted alleles only, and bottom images are from flies expressing the noted alleles in the context of 30 GGGGCC repeats under the same driver. (B) Violin plots of eye degeneration scores of 30R flies expressing the noted UPF1 alleles. See details for details on scoring method. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. ****p < 0.0001. From left to right, n = 108, 54, 101, 15, 60, 15. Data are represented as violin plots with indicated quartiles. (C) Representative fields of view of propidium-iodide-positive iPSNs in the absence (top row) or presence (bottom row) of 10 μM glutamate. Scale bar, 100 μm. (D) Quantification of the relative proportion of propidium-iodide-positive (dead) cells to total cells from images in (C), represented as “% Cell Death.” n = 3 pairs of age- and sex-matched control and C9orf72 iPSNs, 2 replicates per pair. Six fields of view were analyzed for each data point. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. *p < 0.05, ****p < 0.0001. Data are indicated as mean ± SD. See also .

    Article Snippet: Rabbit anti-UPF1 , ProteinTech , Cat#23379-1-AP; RRID: AB_11232421.

    Techniques: Expressing

    (A) Relative abundance of sense repeat RNA following treatment with 0.5 μM SMG1i for the indicated periods of time (24 h 0.1% DMSO treatment used for normalization). n = 4 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. **p < 0.01. (B) Western blot against total UPF1 protein in samples following anti-UPF1 IP from C9-ALS iPSN lysates. Short and long exposures are shown on the top and bottom, respectively. (C) Fold enrichment of sense repeat RNA and ATF4 mRNA relative to GAPDH in IP fraction following anti-UPF1 pulldown as measured by qRT-PCR (input used for normalization). n = 5 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. Data are indicated as mean ± SD. See also .

    Journal: Cell reports

    Article Title: UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction

    doi: 10.1016/j.celrep.2021.108925

    Figure Lengend Snippet: (A) Relative abundance of sense repeat RNA following treatment with 0.5 μM SMG1i for the indicated periods of time (24 h 0.1% DMSO treatment used for normalization). n = 4 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. **p < 0.01. (B) Western blot against total UPF1 protein in samples following anti-UPF1 IP from C9-ALS iPSN lysates. Short and long exposures are shown on the top and bottom, respectively. (C) Fold enrichment of sense repeat RNA and ATF4 mRNA relative to GAPDH in IP fraction following anti-UPF1 pulldown as measured by qRT-PCR (input used for normalization). n = 5 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. Data are indicated as mean ± SD. See also .

    Article Snippet: Rabbit anti-UPF1 , ProteinTech , Cat#23379-1-AP; RRID: AB_11232421.

    Techniques: Western Blot, Quantitative RT-PCR

    (A) Ratio of Nluc to Fluc from HeLa cells stably expressing dual-luciferase reporters (with reading frame noted) and transfected control or UPF1 siRNA. n = 3 biological replicates. Unpaired t tests were used to calculate statistical significance. **p < 0.01, ***p < 0.001. (B) Poly(GP) response in control (left) and C9-ALS (right) iPSNs following OE of GFP or UPF1 as measured by an ELISA assay. n = 3 age- and sex-matched pairs of control and C9orf72 iPSNs, 2 replicates each line. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. (C) Relative abundance of sense repeat RNA in C9-ALS iPSNs OE GFP or UPF1. n = 3 C9-ALS iPSN lines, 2 replicates for each line. Paired t tests were used to calculate statistical significance. Data are indicated as mean ± SD. See also .

    Journal: Cell reports

    Article Title: UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction

    doi: 10.1016/j.celrep.2021.108925

    Figure Lengend Snippet: (A) Ratio of Nluc to Fluc from HeLa cells stably expressing dual-luciferase reporters (with reading frame noted) and transfected control or UPF1 siRNA. n = 3 biological replicates. Unpaired t tests were used to calculate statistical significance. **p < 0.01, ***p < 0.001. (B) Poly(GP) response in control (left) and C9-ALS (right) iPSNs following OE of GFP or UPF1 as measured by an ELISA assay. n = 3 age- and sex-matched pairs of control and C9orf72 iPSNs, 2 replicates each line. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. (C) Relative abundance of sense repeat RNA in C9-ALS iPSNs OE GFP or UPF1. n = 3 C9-ALS iPSN lines, 2 replicates for each line. Paired t tests were used to calculate statistical significance. Data are indicated as mean ± SD. See also .

    Article Snippet: Rabbit anti-UPF1 , ProteinTech , Cat#23379-1-AP; RRID: AB_11232421.

    Techniques: Stable Transfection, Expressing, Luciferase, Transfection, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction

    doi: 10.1016/j.celrep.2021.108925

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit anti-UPF1 , ProteinTech , Cat#23379-1-AP; RRID: AB_11232421.

    Techniques: Recombinant, Clone Assay, Luciferase, Sequencing, Plasmid Preparation, Software, Imaging, Real-time Polymerase Chain Reaction

    The tRF-Leu-AAG promoted PC development by suppressing UPF1.

    Journal: Bioengineered

    Article Title: The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells

    doi: 10.1080/21655979.2022.2064206

    Figure Lengend Snippet: The tRF-Leu-AAG promoted PC development by suppressing UPF1.

    Article Snippet: Subsequently, the proteins were blocked with a quick-blocking solution for 30 min. UPF1 primary antibody (1:1000; 23,379-1-AP, Proteintech) or β-Actin primary antibody (1:5000; 60,008-1-Lg, Proteintech) was overnight incubated at 4°C.

    Techniques: