66883 Search Results


wfdc2  (Novus Biologicals)
90
Novus Biologicals wfdc2
Verification of <t>WFDC2</t> and PEX19 interaction in HK2, podocyte, and glomerular endothelial cells. (A) Relative WFDC2 transcript levels were upregulated in many diabetic nephropathy human and mice datasets in the kidney tubule. (B) Relative FEX19 transcript levels were downregulated in FSGS in the kidney glomeruli in Nephroseq database. (C,D) WFDC2 in the tubule was upregulated, and FEX19 in the glomeruli was downregulated in STZ-induced diabetic mice by qPCR and western blot. (E) Western blot shows WFDC2 overexpression in HK2 cell. (F) PEX19 protein level was decreased with WFDC2 treatment in podocyte and glomerular endothelial cells by western blot. Con, WFDC2 empty vector; OE, WFDC2 overexpression vector; EC, glomerular endothelial cells. (A–C) Values are mean ± SEM; * p ≤ 0.05 between means. (A,B) All the genes were significantly changed in CKD compared with control with p < 0.001.
Wfdc2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wfdc2/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
wfdc2 - by Bioz Stars, 2025-05
90/100 stars
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slc3a2  (Proteintech)
95
Proteintech slc3a2
Verification of <t>WFDC2</t> and PEX19 interaction in HK2, podocyte, and glomerular endothelial cells. (A) Relative WFDC2 transcript levels were upregulated in many diabetic nephropathy human and mice datasets in the kidney tubule. (B) Relative FEX19 transcript levels were downregulated in FSGS in the kidney glomeruli in Nephroseq database. (C,D) WFDC2 in the tubule was upregulated, and FEX19 in the glomeruli was downregulated in STZ-induced diabetic mice by qPCR and western blot. (E) Western blot shows WFDC2 overexpression in HK2 cell. (F) PEX19 protein level was decreased with WFDC2 treatment in podocyte and glomerular endothelial cells by western blot. Con, WFDC2 empty vector; OE, WFDC2 overexpression vector; EC, glomerular endothelial cells. (A–C) Values are mean ± SEM; * p ≤ 0.05 between means. (A,B) All the genes were significantly changed in CKD compared with control with p < 0.001.
Slc3a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
slc3a2 - by Bioz Stars, 2025-05
95/100 stars
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human e2f1 sh  (Addgene inc)
94
Addgene inc human e2f1 sh
Influence of TUBG1, <t>E2F1,</t> and RB1 protein levels on the cytotoxic effect of L12 treatment. (A and B) The MCF10A cell lines used include: Control (MCF10A, non‐modified parental cells), MCF10A sh TUBG (stably expressing TUBG shRNA) and MCF10A sh TUBG TUBG1 (stably co‐expressing TUBG shRNA and a sh‐resistant TUBG1 gene). (A) MCF10A cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. DNA content was measured by nuclear counter to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction (indicative of dead cells). Histograms display the results, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (B) Western blotting (WB) was performed to analyze TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs illustrate relative protein expression (Student's t test, N = 3, * p < .05, ** p < .01). (C and D) The U2OS cell lines used include: Control (U2OS, non‐modified parental cells), U2OS sh E2F1 (transiently expressing E2F1 shRNA) and U2OS E2F1 sh E2F1 (transiently co‐expressing E2F1 sgRNA and a E2F1 gene). U2OS cells were treated with DMSO (vehicle) or 50 nM L12 for 24 h. (C) DNA content was measured to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction. Histograms show representative data, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 4; Student's t test, * p < .01, ** p < .01). (D) WB was used to analyze E2F1 and procaspase 3 protein levels in total lysates using anti‐E2F1 and anti‐procaspase 3 antibodies. GAPDH served as the loading control. Graphs display relative protein expression (Student's t test, N = 4, * p < .05, ** p < .01). The numbers above the blots (WB) represent the normalized intensity of the protein bands. (E and F) The A549 cell lines used include: Control (A549, non‐modified parental cells), A549 sg RB1 (stably expressing RB1 sgRNA) and A549 RB1 sgRNA RB1 (stably co‐expressing RB1 sgRNA and a sg‐resistant RB1 gene). (E) A549 cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. The cell cycle profile and percentage of sub‐G1 cells were determined. Histograms represent the results, and graphs show mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (F) WB analyzed TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs depict relative protein expression (Student's t test, N = 3, * p < .05, **** p < .0001). To ensure accurate comparisons of RB1 protein levels under different conditions, Western blot exposure times were optimized for each experiment to balance signal detection and prevent overexposure, enabling the detection of subtle differences in RB1 expression.
Human E2f1 Sh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
human e2f1 sh - by Bioz Stars, 2025-05
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66883 u  (Millipore)
86
Millipore 66883 u
Influence of TUBG1, <t>E2F1,</t> and RB1 protein levels on the cytotoxic effect of L12 treatment. (A and B) The MCF10A cell lines used include: Control (MCF10A, non‐modified parental cells), MCF10A sh TUBG (stably expressing TUBG shRNA) and MCF10A sh TUBG TUBG1 (stably co‐expressing TUBG shRNA and a sh‐resistant TUBG1 gene). (A) MCF10A cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. DNA content was measured by nuclear counter to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction (indicative of dead cells). Histograms display the results, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (B) Western blotting (WB) was performed to analyze TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs illustrate relative protein expression (Student's t test, N = 3, * p < .05, ** p < .01). (C and D) The U2OS cell lines used include: Control (U2OS, non‐modified parental cells), U2OS sh E2F1 (transiently expressing E2F1 shRNA) and U2OS E2F1 sh E2F1 (transiently co‐expressing E2F1 sgRNA and a E2F1 gene). U2OS cells were treated with DMSO (vehicle) or 50 nM L12 for 24 h. (C) DNA content was measured to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction. Histograms show representative data, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 4; Student's t test, * p < .01, ** p < .01). (D) WB was used to analyze E2F1 and procaspase 3 protein levels in total lysates using anti‐E2F1 and anti‐procaspase 3 antibodies. GAPDH served as the loading control. Graphs display relative protein expression (Student's t test, N = 4, * p < .05, ** p < .01). The numbers above the blots (WB) represent the normalized intensity of the protein bands. (E and F) The A549 cell lines used include: Control (A549, non‐modified parental cells), A549 sg RB1 (stably expressing RB1 sgRNA) and A549 RB1 sgRNA RB1 (stably co‐expressing RB1 sgRNA and a sg‐resistant RB1 gene). (E) A549 cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. The cell cycle profile and percentage of sub‐G1 cells were determined. Histograms represent the results, and graphs show mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (F) WB analyzed TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs depict relative protein expression (Student's t test, N = 3, * p < .05, **** p < .0001). To ensure accurate comparisons of RB1 protein levels under different conditions, Western blot exposure times were optimized for each experiment to balance signal detection and prevent overexposure, enabling the detection of subtle differences in RB1 expression.
66883 U, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/66883 u/product/Millipore
Average 86 stars, based on 1 article reviews
66883 u - by Bioz Stars, 2025-05
86/100 stars
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Image Search Results


Verification of WFDC2 and PEX19 interaction in HK2, podocyte, and glomerular endothelial cells. (A) Relative WFDC2 transcript levels were upregulated in many diabetic nephropathy human and mice datasets in the kidney tubule. (B) Relative FEX19 transcript levels were downregulated in FSGS in the kidney glomeruli in Nephroseq database. (C,D) WFDC2 in the tubule was upregulated, and FEX19 in the glomeruli was downregulated in STZ-induced diabetic mice by qPCR and western blot. (E) Western blot shows WFDC2 overexpression in HK2 cell. (F) PEX19 protein level was decreased with WFDC2 treatment in podocyte and glomerular endothelial cells by western blot. Con, WFDC2 empty vector; OE, WFDC2 overexpression vector; EC, glomerular endothelial cells. (A–C) Values are mean ± SEM; * p ≤ 0.05 between means. (A,B) All the genes were significantly changed in CKD compared with control with p < 0.001.

Journal: Frontiers in Medicine

Article Title: Integrative Informatics Analysis of Transcriptome and Identification of Interacted Genes in the Glomeruli and Tubules in CKD

doi: 10.3389/fmed.2020.615306

Figure Lengend Snippet: Verification of WFDC2 and PEX19 interaction in HK2, podocyte, and glomerular endothelial cells. (A) Relative WFDC2 transcript levels were upregulated in many diabetic nephropathy human and mice datasets in the kidney tubule. (B) Relative FEX19 transcript levels were downregulated in FSGS in the kidney glomeruli in Nephroseq database. (C,D) WFDC2 in the tubule was upregulated, and FEX19 in the glomeruli was downregulated in STZ-induced diabetic mice by qPCR and western blot. (E) Western blot shows WFDC2 overexpression in HK2 cell. (F) PEX19 protein level was decreased with WFDC2 treatment in podocyte and glomerular endothelial cells by western blot. Con, WFDC2 empty vector; OE, WFDC2 overexpression vector; EC, glomerular endothelial cells. (A–C) Values are mean ± SEM; * p ≤ 0.05 between means. (A,B) All the genes were significantly changed in CKD compared with control with p < 0.001.

Article Snippet: The following antibodies were used: WFDC2 (rabbit monoclonal HE4/WFDC2, Catalog # NBP2-66883; Novus Biologicals), PEX19 (PEX19 monoclonal antibody (GT554), Catalog # MA5-17266; Invitrogen), and GAPDH (mouse monoclonal antibody, Catalog # G8795-100UL; Sigma).

Techniques: Western Blot, Over Expression, Plasmid Preparation

Influence of TUBG1, E2F1, and RB1 protein levels on the cytotoxic effect of L12 treatment. (A and B) The MCF10A cell lines used include: Control (MCF10A, non‐modified parental cells), MCF10A sh TUBG (stably expressing TUBG shRNA) and MCF10A sh TUBG TUBG1 (stably co‐expressing TUBG shRNA and a sh‐resistant TUBG1 gene). (A) MCF10A cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. DNA content was measured by nuclear counter to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction (indicative of dead cells). Histograms display the results, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (B) Western blotting (WB) was performed to analyze TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs illustrate relative protein expression (Student's t test, N = 3, * p < .05, ** p < .01). (C and D) The U2OS cell lines used include: Control (U2OS, non‐modified parental cells), U2OS sh E2F1 (transiently expressing E2F1 shRNA) and U2OS E2F1 sh E2F1 (transiently co‐expressing E2F1 sgRNA and a E2F1 gene). U2OS cells were treated with DMSO (vehicle) or 50 nM L12 for 24 h. (C) DNA content was measured to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction. Histograms show representative data, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 4; Student's t test, * p < .01, ** p < .01). (D) WB was used to analyze E2F1 and procaspase 3 protein levels in total lysates using anti‐E2F1 and anti‐procaspase 3 antibodies. GAPDH served as the loading control. Graphs display relative protein expression (Student's t test, N = 4, * p < .05, ** p < .01). The numbers above the blots (WB) represent the normalized intensity of the protein bands. (E and F) The A549 cell lines used include: Control (A549, non‐modified parental cells), A549 sg RB1 (stably expressing RB1 sgRNA) and A549 RB1 sgRNA RB1 (stably co‐expressing RB1 sgRNA and a sg‐resistant RB1 gene). (E) A549 cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. The cell cycle profile and percentage of sub‐G1 cells were determined. Histograms represent the results, and graphs show mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (F) WB analyzed TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs depict relative protein expression (Student's t test, N = 3, * p < .05, **** p < .0001). To ensure accurate comparisons of RB1 protein levels under different conditions, Western blot exposure times were optimized for each experiment to balance signal detection and prevent overexposure, enabling the detection of subtle differences in RB1 expression.

Journal: The FASEB Journal

Article Title: Targeting TUBG1 in RB1 ‐negative tumors

doi: 10.1096/fj.202403180RR

Figure Lengend Snippet: Influence of TUBG1, E2F1, and RB1 protein levels on the cytotoxic effect of L12 treatment. (A and B) The MCF10A cell lines used include: Control (MCF10A, non‐modified parental cells), MCF10A sh TUBG (stably expressing TUBG shRNA) and MCF10A sh TUBG TUBG1 (stably co‐expressing TUBG shRNA and a sh‐resistant TUBG1 gene). (A) MCF10A cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. DNA content was measured by nuclear counter to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction (indicative of dead cells). Histograms display the results, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (B) Western blotting (WB) was performed to analyze TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs illustrate relative protein expression (Student's t test, N = 3, * p < .05, ** p < .01). (C and D) The U2OS cell lines used include: Control (U2OS, non‐modified parental cells), U2OS sh E2F1 (transiently expressing E2F1 shRNA) and U2OS E2F1 sh E2F1 (transiently co‐expressing E2F1 sgRNA and a E2F1 gene). U2OS cells were treated with DMSO (vehicle) or 50 nM L12 for 24 h. (C) DNA content was measured to determine the cell cycle profile and the percentage of cells in the sub‐G1 fraction. Histograms show representative data, and graphs summarize the mean ± SD percentages of sub‐G1 cells ( N = 4; Student's t test, * p < .01, ** p < .01). (D) WB was used to analyze E2F1 and procaspase 3 protein levels in total lysates using anti‐E2F1 and anti‐procaspase 3 antibodies. GAPDH served as the loading control. Graphs display relative protein expression (Student's t test, N = 4, * p < .05, ** p < .01). The numbers above the blots (WB) represent the normalized intensity of the protein bands. (E and F) The A549 cell lines used include: Control (A549, non‐modified parental cells), A549 sg RB1 (stably expressing RB1 sgRNA) and A549 RB1 sgRNA RB1 (stably co‐expressing RB1 sgRNA and a sg‐resistant RB1 gene). (E) A549 cells were treated with DMSO (vehicle) or the indicated concentrations of L12 for 24 h. The cell cycle profile and percentage of sub‐G1 cells were determined. Histograms represent the results, and graphs show mean ± SD percentages of sub‐G1 cells ( N = 3; two‐way ANOVA, **** p < .0001). (F) WB analyzed TUBG and RB1 protein levels in total lysates using anti‐TUBG and anti‐RB1 antibodies. Actin served as the loading control. Graphs depict relative protein expression (Student's t test, N = 3, * p < .05, **** p < .0001). To ensure accurate comparisons of RB1 protein levels under different conditions, Western blot exposure times were optimized for each experiment to balance signal detection and prevent overexposure, enabling the detection of subtle differences in RB1 expression.

Article Snippet: Human TUBG1 single guide (sg; RRID:Addgene_104437) and short hairpin (sh) RNA (RRID:Addgene_87955), human sg‐resistant pcDNA3‐ TUBG1 (RRID:Addgene_104433), human E2F1 sh (RRID:Addgene_66883) and pcDNA3‐ TUBG2 (RRID:Addgene_171966) were prepared as previously reported., , , , The human pSG5L‐ RB1 and pcDNA‐ E2F1 constructs were kindly provided by Dr. W. Sellers (RRID:Addgene_10720 ) and Dr. J.R. Nevins (Duke University, USA ), respectively.

Techniques: Control, Modification, Stable Transfection, Expressing, shRNA, Western Blot