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  • 92
    Proteintech anti cyclin y
    Cyclin Y, but not CDK14, is expressed in colonic epithelial cells. ( a ) Representative immunostaining of <t>cyclin</t> <t>Y</t> (red, with nuclei in blue) in the colon of wild-type mice. The magnified inset on the right highlights cyclin Y localization to the plasma membrane of crypt base intestinal epithelial cells (arrowheads). ( b – e ) Gene expression analysis of CCNY ( b ), CCNYL1 ( c ), CDK14 ( d ), and CDK16 ( e ) in a single-cell RNA-seq dataset of intestinal epithelial cells from ulcerative colitis and control patients (Single Cell Portal accession SCP259). Peak expression of CCNY and CDK16 was observed in epithelial stem and progenitor cells, whereas CCNYL1 was more broadly expressed. Additional information on cell clusters can be found in . ( f ) CDK14 staining (green, with nuclei in blue and vimentin in red) in wild-type mouse colon. CDK14 was detected in stromal cells (arrowheads), but not intestinal epithelial cells (*).
    Anti Cyclin Y, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin y/product/Proteintech
    Average 92 stars, based on 1 article reviews
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    anti cyclin y - by Bioz Stars, 2024-06
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    92
    Proteintech 66865 1 ig
    Cyclin Y, but not CDK14, is expressed in colonic epithelial cells. ( a ) Representative immunostaining of <t>cyclin</t> <t>Y</t> (red, with nuclei in blue) in the colon of wild-type mice. The magnified inset on the right highlights cyclin Y localization to the plasma membrane of crypt base intestinal epithelial cells (arrowheads). ( b – e ) Gene expression analysis of CCNY ( b ), CCNYL1 ( c ), CDK14 ( d ), and CDK16 ( e ) in a single-cell RNA-seq dataset of intestinal epithelial cells from ulcerative colitis and control patients (Single Cell Portal accession SCP259). Peak expression of CCNY and CDK16 was observed in epithelial stem and progenitor cells, whereas CCNYL1 was more broadly expressed. Additional information on cell clusters can be found in . ( f ) CDK14 staining (green, with nuclei in blue and vimentin in red) in wild-type mouse colon. CDK14 was detected in stromal cells (arrowheads), but not intestinal epithelial cells (*).
    66865 1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/66865 1 ig/product/Proteintech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    66865 1 ig - by Bioz Stars, 2024-06
    92/100 stars
      Buy from Supplier

    92
    Proteintech mouse monoclonal anti cyclin y 2c9e3
    Antibodies used for western blots.
    Mouse Monoclonal Anti Cyclin Y 2c9e3, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti cyclin y 2c9e3/product/Proteintech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti cyclin y 2c9e3 - by Bioz Stars, 2024-06
    92/100 stars
      Buy from Supplier

    92
    Proteintech anti ccny
    Antibodies used for western blots.
    Anti Ccny, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ccny/product/Proteintech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ccny - by Bioz Stars, 2024-06
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    92
    Proteintech rabbit polyclonal anti ccny antibody
    Antibodies used for western blots.
    Rabbit Polyclonal Anti Ccny Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti ccny antibody/product/Proteintech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti ccny antibody - by Bioz Stars, 2024-06
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    Image Search Results


    Cyclin Y, but not CDK14, is expressed in colonic epithelial cells. ( a ) Representative immunostaining of cyclin Y (red, with nuclei in blue) in the colon of wild-type mice. The magnified inset on the right highlights cyclin Y localization to the plasma membrane of crypt base intestinal epithelial cells (arrowheads). ( b – e ) Gene expression analysis of CCNY ( b ), CCNYL1 ( c ), CDK14 ( d ), and CDK16 ( e ) in a single-cell RNA-seq dataset of intestinal epithelial cells from ulcerative colitis and control patients (Single Cell Portal accession SCP259). Peak expression of CCNY and CDK16 was observed in epithelial stem and progenitor cells, whereas CCNYL1 was more broadly expressed. Additional information on cell clusters can be found in . ( f ) CDK14 staining (green, with nuclei in blue and vimentin in red) in wild-type mouse colon. CDK14 was detected in stromal cells (arrowheads), but not intestinal epithelial cells (*).

    Journal: Cells

    Article Title: The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis

    doi: 10.3390/cells10092330

    Figure Lengend Snippet: Cyclin Y, but not CDK14, is expressed in colonic epithelial cells. ( a ) Representative immunostaining of cyclin Y (red, with nuclei in blue) in the colon of wild-type mice. The magnified inset on the right highlights cyclin Y localization to the plasma membrane of crypt base intestinal epithelial cells (arrowheads). ( b – e ) Gene expression analysis of CCNY ( b ), CCNYL1 ( c ), CDK14 ( d ), and CDK16 ( e ) in a single-cell RNA-seq dataset of intestinal epithelial cells from ulcerative colitis and control patients (Single Cell Portal accession SCP259). Peak expression of CCNY and CDK16 was observed in epithelial stem and progenitor cells, whereas CCNYL1 was more broadly expressed. Additional information on cell clusters can be found in . ( f ) CDK14 staining (green, with nuclei in blue and vimentin in red) in wild-type mouse colon. CDK14 was detected in stromal cells (arrowheads), but not intestinal epithelial cells (*).

    Article Snippet: Membranes were incubated with the following antibodies: anti-cyclin Y (rabbit, 1:5000, 18042-1-AP, Proteintech, Rosemont, IL, USA; or rabbit, 1:200, HPA036290, Sigma-Aldrich, Darmstadt, Germany), anti-CDK14 (mouse, 1:200, sc-376366, Santa Cruz Biotechnology, Dallas, TX, USA), anti-LC3B (rabbit, 1:1000, 2775, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 Sp1490 (rabbit, 1:1000, 2568T, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 (mouse, 1:1000, sc-25317, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-tubulin (mouse, 1:5000, NB100-690, Novus Biologicals, Centennial, CO, USA), anti-transferrin receptor/CD71 (mouse, 1:1000, sc-65882, Santa Cruz Biotechnology, Dallas, TX, USA); followed by anti-mouse or anti-rabbit NIR fluorophore-conjugated secondary antibodies (1:20,000, LI-COR Biotechnology, Lincoln, NE, USA).

    Techniques: Immunostaining, Expressing, RNA Sequencing Assay, Staining

    Ccny Δ IEC mice do not display altered colitis susceptibility or injury repair. ( a ) Immunoblot validation of loss of cyclin Y (green) in colonic mucosal scrapings of two Ccny Δ IEC mice and a wild-type littermate control. Tubulin (red) was used as a loading control. ( b ) Body weight change and ( c ) clinical disease activity following dextran sulfate sodium (DSS)-induced acute colitis (5 days). ( d ) Body weight change and ( e ) clinical disease activity during the recovery from DSS colitis (10 days). In these assays, Ccny Δ IEC mice were indistinguishable from littermate controls.

    Journal: Cells

    Article Title: The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis

    doi: 10.3390/cells10092330

    Figure Lengend Snippet: Ccny Δ IEC mice do not display altered colitis susceptibility or injury repair. ( a ) Immunoblot validation of loss of cyclin Y (green) in colonic mucosal scrapings of two Ccny Δ IEC mice and a wild-type littermate control. Tubulin (red) was used as a loading control. ( b ) Body weight change and ( c ) clinical disease activity following dextran sulfate sodium (DSS)-induced acute colitis (5 days). ( d ) Body weight change and ( e ) clinical disease activity during the recovery from DSS colitis (10 days). In these assays, Ccny Δ IEC mice were indistinguishable from littermate controls.

    Article Snippet: Membranes were incubated with the following antibodies: anti-cyclin Y (rabbit, 1:5000, 18042-1-AP, Proteintech, Rosemont, IL, USA; or rabbit, 1:200, HPA036290, Sigma-Aldrich, Darmstadt, Germany), anti-CDK14 (mouse, 1:200, sc-376366, Santa Cruz Biotechnology, Dallas, TX, USA), anti-LC3B (rabbit, 1:1000, 2775, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 Sp1490 (rabbit, 1:1000, 2568T, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 (mouse, 1:1000, sc-25317, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-tubulin (mouse, 1:5000, NB100-690, Novus Biologicals, Centennial, CO, USA), anti-transferrin receptor/CD71 (mouse, 1:1000, sc-65882, Santa Cruz Biotechnology, Dallas, TX, USA); followed by anti-mouse or anti-rabbit NIR fluorophore-conjugated secondary antibodies (1:20,000, LI-COR Biotechnology, Lincoln, NE, USA).

    Techniques: Western Blot, Activity Assay

    CCNY depletion does not affect Wnt signaling in intestinal epithelial cells. ( a , b ) Wnt activity upon cyclin Y loss-of-function by RNA interference with two siRNAs (Y #1/2) was assessed using the TOPflash luciferase reporter assay in 293T ( a ) and HCT116 cells ( b ). Where indicated, cells were treated with Wnt3a conditioned media. Data were analyzed by Dunnett’s post hoc test following one-way ANOVA, and statistically significant results are indicated as * p < 0.05 and ** p < 0.01 (versus siCo–Wnt3a). ( c ) Immunoblot analysis of active LRP6 (Sp1490) following cyclin Y loss-of-function and Wnt stimulation. The ratio of Sp1490 versus total LRP6 was normalized to the untreated siControl condition. Wnt-induced LRP6 phosphorylation was not affected by CCNY depletion.

    Journal: Cells

    Article Title: The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis

    doi: 10.3390/cells10092330

    Figure Lengend Snippet: CCNY depletion does not affect Wnt signaling in intestinal epithelial cells. ( a , b ) Wnt activity upon cyclin Y loss-of-function by RNA interference with two siRNAs (Y #1/2) was assessed using the TOPflash luciferase reporter assay in 293T ( a ) and HCT116 cells ( b ). Where indicated, cells were treated with Wnt3a conditioned media. Data were analyzed by Dunnett’s post hoc test following one-way ANOVA, and statistically significant results are indicated as * p < 0.05 and ** p < 0.01 (versus siCo–Wnt3a). ( c ) Immunoblot analysis of active LRP6 (Sp1490) following cyclin Y loss-of-function and Wnt stimulation. The ratio of Sp1490 versus total LRP6 was normalized to the untreated siControl condition. Wnt-induced LRP6 phosphorylation was not affected by CCNY depletion.

    Article Snippet: Membranes were incubated with the following antibodies: anti-cyclin Y (rabbit, 1:5000, 18042-1-AP, Proteintech, Rosemont, IL, USA; or rabbit, 1:200, HPA036290, Sigma-Aldrich, Darmstadt, Germany), anti-CDK14 (mouse, 1:200, sc-376366, Santa Cruz Biotechnology, Dallas, TX, USA), anti-LC3B (rabbit, 1:1000, 2775, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 Sp1490 (rabbit, 1:1000, 2568T, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 (mouse, 1:1000, sc-25317, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-tubulin (mouse, 1:5000, NB100-690, Novus Biologicals, Centennial, CO, USA), anti-transferrin receptor/CD71 (mouse, 1:1000, sc-65882, Santa Cruz Biotechnology, Dallas, TX, USA); followed by anti-mouse or anti-rabbit NIR fluorophore-conjugated secondary antibodies (1:20,000, LI-COR Biotechnology, Lincoln, NE, USA).

    Techniques: Activity Assay, Luciferase, Reporter Assay, Western Blot

    Loss of cyclin Y does not alter intestinal epithelial proliferation. ( a , b ) Cell proliferation of HCT116 cells upon cyclin Y loss-of-function by RNA interference was assessed with the MTT tetrazolium dye assay over a 96-h period. Wnt pathway activity was modulated by treatment with control conditioned media ( a ) or Wnt agonist Wnt3a ( b ). The dashed line indicates the average MTT absorbance of the siControl condition; a.u., arbitrary units. ( c ) Immunostaining of proliferation marker Ki67 (red, with nuclei in blue) in HCT116 cells following CCNY depletion for 48 h. ( d ) Representative immunostaining of Ki67 (red, with nuclei in blue) in the colon of a wild-type mouse. The Ki67-positive zone demarcates the crypt proliferative compartment. ( e – g ) Quantification of total crypt length and the crypt proliferative compartment in the distal colon as a fraction of the total crypt length in ( e ) unchallenged mice, ( f ) mice with acute DSS-induced colitis (5 days), and ( g ) mice following recovery from DSS colitis (10 days). Data points indicate individual animals. Only samples with a sufficiently high number of correctly aligned crypts were included in the analysis. No statistically significant results were observed between the groups (one-way ANOVA with Tukey’s post hoc comparison).

    Journal: Cells

    Article Title: The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis

    doi: 10.3390/cells10092330

    Figure Lengend Snippet: Loss of cyclin Y does not alter intestinal epithelial proliferation. ( a , b ) Cell proliferation of HCT116 cells upon cyclin Y loss-of-function by RNA interference was assessed with the MTT tetrazolium dye assay over a 96-h period. Wnt pathway activity was modulated by treatment with control conditioned media ( a ) or Wnt agonist Wnt3a ( b ). The dashed line indicates the average MTT absorbance of the siControl condition; a.u., arbitrary units. ( c ) Immunostaining of proliferation marker Ki67 (red, with nuclei in blue) in HCT116 cells following CCNY depletion for 48 h. ( d ) Representative immunostaining of Ki67 (red, with nuclei in blue) in the colon of a wild-type mouse. The Ki67-positive zone demarcates the crypt proliferative compartment. ( e – g ) Quantification of total crypt length and the crypt proliferative compartment in the distal colon as a fraction of the total crypt length in ( e ) unchallenged mice, ( f ) mice with acute DSS-induced colitis (5 days), and ( g ) mice following recovery from DSS colitis (10 days). Data points indicate individual animals. Only samples with a sufficiently high number of correctly aligned crypts were included in the analysis. No statistically significant results were observed between the groups (one-way ANOVA with Tukey’s post hoc comparison).

    Article Snippet: Membranes were incubated with the following antibodies: anti-cyclin Y (rabbit, 1:5000, 18042-1-AP, Proteintech, Rosemont, IL, USA; or rabbit, 1:200, HPA036290, Sigma-Aldrich, Darmstadt, Germany), anti-CDK14 (mouse, 1:200, sc-376366, Santa Cruz Biotechnology, Dallas, TX, USA), anti-LC3B (rabbit, 1:1000, 2775, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 Sp1490 (rabbit, 1:1000, 2568T, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 (mouse, 1:1000, sc-25317, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-tubulin (mouse, 1:5000, NB100-690, Novus Biologicals, Centennial, CO, USA), anti-transferrin receptor/CD71 (mouse, 1:1000, sc-65882, Santa Cruz Biotechnology, Dallas, TX, USA); followed by anti-mouse or anti-rabbit NIR fluorophore-conjugated secondary antibodies (1:20,000, LI-COR Biotechnology, Lincoln, NE, USA).

    Techniques: Activity Assay, Immunostaining, Marker

    CCNY does not regulate autophagy in glucose-starved intestinal epithelial cells. ( a ) Immunofluorescence analysis of endogenous autophagosome marker LC3-II in HCT116 cells upon cyclin Y loss-of-function by RNA interference. Cells were maintained for 16 h in complete media or glucose starvation media with 100 nM Bafilomycin A1. ( b ) The average number of LC3 dots per cell in panel ( a ) was quantified by computer-assisted image analysis ( n ≥ 6 images per condition, with ≥149 cells per image). Statistical analysis was performed using mixed-effects analysis (two-way ANOVA) followed by Dunnett’s post hoc test, and is presented as follows: * p < 0.05 and *** p < 0.001 versus complete media; ## p < 0.01 and ### p < 0.001 versus siControl.

    Journal: Cells

    Article Title: The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis

    doi: 10.3390/cells10092330

    Figure Lengend Snippet: CCNY does not regulate autophagy in glucose-starved intestinal epithelial cells. ( a ) Immunofluorescence analysis of endogenous autophagosome marker LC3-II in HCT116 cells upon cyclin Y loss-of-function by RNA interference. Cells were maintained for 16 h in complete media or glucose starvation media with 100 nM Bafilomycin A1. ( b ) The average number of LC3 dots per cell in panel ( a ) was quantified by computer-assisted image analysis ( n ≥ 6 images per condition, with ≥149 cells per image). Statistical analysis was performed using mixed-effects analysis (two-way ANOVA) followed by Dunnett’s post hoc test, and is presented as follows: * p < 0.05 and *** p < 0.001 versus complete media; ## p < 0.01 and ### p < 0.001 versus siControl.

    Article Snippet: Membranes were incubated with the following antibodies: anti-cyclin Y (rabbit, 1:5000, 18042-1-AP, Proteintech, Rosemont, IL, USA; or rabbit, 1:200, HPA036290, Sigma-Aldrich, Darmstadt, Germany), anti-CDK14 (mouse, 1:200, sc-376366, Santa Cruz Biotechnology, Dallas, TX, USA), anti-LC3B (rabbit, 1:1000, 2775, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 Sp1490 (rabbit, 1:1000, 2568T, Cell Signaling Technology, Danvers, MA, USA), anti-LRP6 (mouse, 1:1000, sc-25317, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-tubulin (mouse, 1:5000, NB100-690, Novus Biologicals, Centennial, CO, USA), anti-transferrin receptor/CD71 (mouse, 1:1000, sc-65882, Santa Cruz Biotechnology, Dallas, TX, USA); followed by anti-mouse or anti-rabbit NIR fluorophore-conjugated secondary antibodies (1:20,000, LI-COR Biotechnology, Lincoln, NE, USA).

    Techniques: Immunofluorescence, Marker

    Antibodies used for western blots.

    Journal: Nature Communications

    Article Title: AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy

    doi: 10.1038/s41467-020-14812-0

    Figure Lengend Snippet: Antibodies used for western blots.

    Article Snippet: mouse monoclonal anti-Cyclin Y (2C9E3) , 1:50 , Proteintech, 66865-1-Ig.

    Techniques: Western Blot

    Antibodies used for immunofluorescence staining.

    Journal: Nature Communications

    Article Title: AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy

    doi: 10.1038/s41467-020-14812-0

    Figure Lengend Snippet: Antibodies used for immunofluorescence staining.

    Article Snippet: mouse monoclonal anti-Cyclin Y (2C9E3) , 1:50 , Proteintech, 66865-1-Ig.

    Techniques: Immunofluorescence, Staining