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  • 93
    Proteintech glucose 6 phosphatase
    Livers from 12-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, <t>glucose-6-phosphatase</t> catalytic subunit <t>(G6PC),</t> glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver glycogen (mg·g -1 ) in (B) sedentary mice prior to acute exercise, (C) mice following 30-minutes of exercise, and (D) mice refed following acute exercise. Muscle glycogen (mg·g -1 ) in (E) sedentary mice prior to acute exercise, (F) mice following 30-minutes of exercise, and (G) mice refed following acute exercise. (H) Kidney glycogen (mg·g -1 ) in sedentary mice. (I) Kidney G6PC, FBP, and PCK1 as determined by immunoblotting and representative immunoblots. n = 5-9 per genotype. Data are mean ± SEM. *p<0.05 and **p<0.01.
    Glucose 6 Phosphatase, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose 6 phosphatase/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose 6 phosphatase - by Bioz Stars, 2024-06
    93/100 stars
      Buy from Supplier

    93
    Proteintech cat 22169 1 ap
    Livers from 12-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, <t>glucose-6-phosphatase</t> catalytic subunit <t>(G6PC),</t> glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver glycogen (mg·g -1 ) in (B) sedentary mice prior to acute exercise, (C) mice following 30-minutes of exercise, and (D) mice refed following acute exercise. Muscle glycogen (mg·g -1 ) in (E) sedentary mice prior to acute exercise, (F) mice following 30-minutes of exercise, and (G) mice refed following acute exercise. (H) Kidney glycogen (mg·g -1 ) in sedentary mice. (I) Kidney G6PC, FBP, and PCK1 as determined by immunoblotting and representative immunoblots. n = 5-9 per genotype. Data are mean ± SEM. *p<0.05 and **p<0.01.
    Cat 22169 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cat 22169 1 ap/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cat 22169 1 ap - by Bioz Stars, 2024-06
    93/100 stars
      Buy from Supplier

    93
    Proteintech 22169 1 ap
    Livers from 12-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, <t>glucose-6-phosphatase</t> catalytic subunit <t>(G6PC),</t> glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver glycogen (mg·g -1 ) in (B) sedentary mice prior to acute exercise, (C) mice following 30-minutes of exercise, and (D) mice refed following acute exercise. Muscle glycogen (mg·g -1 ) in (E) sedentary mice prior to acute exercise, (F) mice following 30-minutes of exercise, and (G) mice refed following acute exercise. (H) Kidney glycogen (mg·g -1 ) in sedentary mice. (I) Kidney G6PC, FBP, and PCK1 as determined by immunoblotting and representative immunoblots. n = 5-9 per genotype. Data are mean ± SEM. *p<0.05 and **p<0.01.
    22169 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/22169 1 ap/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    22169 1 ap - by Bioz Stars, 2024-06
    93/100 stars
      Buy from Supplier

    88
    Addgene inc phdlx2 n174
    Livers from 12-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, <t>glucose-6-phosphatase</t> catalytic subunit <t>(G6PC),</t> glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver glycogen (mg·g -1 ) in (B) sedentary mice prior to acute exercise, (C) mice following 30-minutes of exercise, and (D) mice refed following acute exercise. Muscle glycogen (mg·g -1 ) in (E) sedentary mice prior to acute exercise, (F) mice following 30-minutes of exercise, and (G) mice refed following acute exercise. (H) Kidney glycogen (mg·g -1 ) in sedentary mice. (I) Kidney G6PC, FBP, and PCK1 as determined by immunoblotting and representative immunoblots. n = 5-9 per genotype. Data are mean ± SEM. *p<0.05 and **p<0.01.
    Phdlx2 N174, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phdlx2 n174/product/Addgene inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phdlx2 n174 - by Bioz Stars, 2024-06
    88/100 stars
      Buy from Supplier

    Image Search Results


    Livers from 12-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, glucose-6-phosphatase catalytic subunit (G6PC), glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver glycogen (mg·g -1 ) in (B) sedentary mice prior to acute exercise, (C) mice following 30-minutes of exercise, and (D) mice refed following acute exercise. Muscle glycogen (mg·g -1 ) in (E) sedentary mice prior to acute exercise, (F) mice following 30-minutes of exercise, and (G) mice refed following acute exercise. (H) Kidney glycogen (mg·g -1 ) in sedentary mice. (I) Kidney G6PC, FBP, and PCK1 as determined by immunoblotting and representative immunoblots. n = 5-9 per genotype. Data are mean ± SEM. *p<0.05 and **p<0.01.

    Journal: bioRxiv

    Article Title: Loss of hepatic phosphoenolpyruvate carboxykinase 1 dysregulates metabolic responses to acute exercise but enhances adaptations to exercise training in mice

    doi: 10.1101/2022.09.22.509117

    Figure Lengend Snippet: Livers from 12-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, glucose-6-phosphatase catalytic subunit (G6PC), glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver glycogen (mg·g -1 ) in (B) sedentary mice prior to acute exercise, (C) mice following 30-minutes of exercise, and (D) mice refed following acute exercise. Muscle glycogen (mg·g -1 ) in (E) sedentary mice prior to acute exercise, (F) mice following 30-minutes of exercise, and (G) mice refed following acute exercise. (H) Kidney glycogen (mg·g -1 ) in sedentary mice. (I) Kidney G6PC, FBP, and PCK1 as determined by immunoblotting and representative immunoblots. n = 5-9 per genotype. Data are mean ± SEM. *p<0.05 and **p<0.01.

    Article Snippet: Immunoblotting was performed with the following primary antibodies: β-hydroxybutyrate dehydrogenase (BDH1; Proteintech, Rosemont, IL, USA, 15417-1-AP; RRID:AB_2274683), fructose 1,6-bisphosphatase (FBP; Proteintech; 12842-1-AP; RRID:AB_2103572), glucose-6-phosphatase (G6PC; Proteintech, 22169-1-AP; RRID:AB_2879015), glycogen phosphorylase (PYGL; Proteintech, 15851-1-AP; RRID:AB_2175014), phospho-glycogen synthase (Ser641) (pGS S641 ; Cell Signaling Technology, 3891; RRID:AB_2116390), glycogen synthase 2 (GS; Proteintech, 22371-1-AP; RRID:AB_2879091), mitochondrial encoded cytochrome c oxidase II (MT-CO2, Proteintech, 55070-1-AP; RRID:AB_10859832), NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 (NDUFS4, Abcam, ab139178; RRID:AB_2922810), cytosolic phosphoenolpyruvate carboxykinase (PCK1; Proteintech, 16754-1-AP; RRID:AB_2160031), succinyl-CoA:3-oxoacid-CoA transferase (SCOT, Proteintech, 12175-1-AP; RRID:AB_2157444), Total OXPHOS Rodent WB Antibody Cocktail (Abcam, Cambridge, MA, USA, ab110413; RRID:AB_2629281), and 3-hydroxymethylglutaryl-CoA synthase 2 (HMGCS2; Cell Signaling Technology, 20940S; RRID:AB_2798853).

    Techniques: Knock-Out, Western Blot

    Following 6 weeks of exercise training protocols, livers from 18-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, glucose-6-phosphatase catalytic subunit (G6PC), glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver (B) glycogen (mg·mg -1 ), (C) phospholipids (PL; µg·mg -1 ), (D) diacylglycerides (DAG; µg·mg -1 ), and (E) triacylglycerides (TAG; µg·mg -1 ). (E) Liver respiratory chain complexes 1-5 (CI-CIV) as determined by immunoblotting and representative immunoblots. (F) Liver acetoacetate (AcAc; nmol·g -1 ), β-hydroxybutyrate (βOHB; nmol·g -1 ), and total ketone bodies (TKB; nmol·g -1 ). (G) Liver 3-hydroxymethylglutaryl-CoA synthase 2 (HMGCS2) and β-hydroxybutyrate dehydrogenase (BDH1) as determined by immunoblotting and representative immunoblots. n = 6-9 per group. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. ND, not determined.

    Journal: bioRxiv

    Article Title: Loss of hepatic phosphoenolpyruvate carboxykinase 1 dysregulates metabolic responses to acute exercise but enhances adaptations to exercise training in mice

    doi: 10.1101/2022.09.22.509117

    Figure Lengend Snippet: Following 6 weeks of exercise training protocols, livers from 18-week-old mice with a hepatic-specific knockout (KO) of phosphoenolpyruvate carboxykinase 1 (PCK1) and wild type (WT) littermates were obtained during fasted, sedentary mice to determine (A) liver PCK1, glucose-6-phosphatase catalytic subunit (G6PC), glycogen phosphorylase (PYGL), phosphorylated glycogen synthase-to-total glycogen synthase ratio (pGS S641 /GS), and fructose 1,6-biphosphatase (FBP) protein via immunoblotting. Liver (B) glycogen (mg·mg -1 ), (C) phospholipids (PL; µg·mg -1 ), (D) diacylglycerides (DAG; µg·mg -1 ), and (E) triacylglycerides (TAG; µg·mg -1 ). (E) Liver respiratory chain complexes 1-5 (CI-CIV) as determined by immunoblotting and representative immunoblots. (F) Liver acetoacetate (AcAc; nmol·g -1 ), β-hydroxybutyrate (βOHB; nmol·g -1 ), and total ketone bodies (TKB; nmol·g -1 ). (G) Liver 3-hydroxymethylglutaryl-CoA synthase 2 (HMGCS2) and β-hydroxybutyrate dehydrogenase (BDH1) as determined by immunoblotting and representative immunoblots. n = 6-9 per group. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. ND, not determined.

    Article Snippet: Immunoblotting was performed with the following primary antibodies: β-hydroxybutyrate dehydrogenase (BDH1; Proteintech, Rosemont, IL, USA, 15417-1-AP; RRID:AB_2274683), fructose 1,6-bisphosphatase (FBP; Proteintech; 12842-1-AP; RRID:AB_2103572), glucose-6-phosphatase (G6PC; Proteintech, 22169-1-AP; RRID:AB_2879015), glycogen phosphorylase (PYGL; Proteintech, 15851-1-AP; RRID:AB_2175014), phospho-glycogen synthase (Ser641) (pGS S641 ; Cell Signaling Technology, 3891; RRID:AB_2116390), glycogen synthase 2 (GS; Proteintech, 22371-1-AP; RRID:AB_2879091), mitochondrial encoded cytochrome c oxidase II (MT-CO2, Proteintech, 55070-1-AP; RRID:AB_10859832), NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 (NDUFS4, Abcam, ab139178; RRID:AB_2922810), cytosolic phosphoenolpyruvate carboxykinase (PCK1; Proteintech, 16754-1-AP; RRID:AB_2160031), succinyl-CoA:3-oxoacid-CoA transferase (SCOT, Proteintech, 12175-1-AP; RRID:AB_2157444), Total OXPHOS Rodent WB Antibody Cocktail (Abcam, Cambridge, MA, USA, ab110413; RRID:AB_2629281), and 3-hydroxymethylglutaryl-CoA synthase 2 (HMGCS2; Cell Signaling Technology, 20940S; RRID:AB_2798853).

    Techniques: Knock-Out, Western Blot