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  • 91
    Proteintech anti human tdg antibody
    Anti Human Tdg Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Proteintech rabbit anti human tdg
    Rabbit Anti Human Tdg, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti tdg ab
    Anti Tdg Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Addgene inc crispra
    a) An NPC donor line heterozygous for index SNP (rs11709284) was transduced with lentivirus carrying the indicated gRNA, and dSpCas9:KRAB (CRISPRi) or <t>dSpCas9:VP64</t> <t>(CRISPRa).</t> Cells were incubated for four days, followed by an eight hour treatment with 1.5 mM LiCl. GNL3 expression was analyzed by RT-qPCR. Data normalized to neg. control gRNA (non-targeting, dashed line). n=4 qPCR replicates, significance determined by two sample t-test. * p<0.05, ** p<0.01, *** p<0.001. b) Proportion of NPCs in S-phase expressing indicated dSpCas9 and gRNAs at baseline, analyzed by high content imaging, as in . n=7 wells per condition, nine tiled images per well, significance determined by two sample t-test. c) Change in the proportion of NPCs in S-phase in response to 1.5mM LiCl, while expressing indicated dSpCas9 and gRNAs. n=7 wells per condition, significance determined by two sample t-test.
    Crispra, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crispra - by Bioz Stars, 2024-05
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    91
    Proteintech antibodies against tdg
    a) An NPC donor line heterozygous for index SNP (rs11709284) was transduced with lentivirus carrying the indicated gRNA, and dSpCas9:KRAB (CRISPRi) or <t>dSpCas9:VP64</t> <t>(CRISPRa).</t> Cells were incubated for four days, followed by an eight hour treatment with 1.5 mM LiCl. GNL3 expression was analyzed by RT-qPCR. Data normalized to neg. control gRNA (non-targeting, dashed line). n=4 qPCR replicates, significance determined by two sample t-test. * p<0.05, ** p<0.01, *** p<0.001. b) Proportion of NPCs in S-phase expressing indicated dSpCas9 and gRNAs at baseline, analyzed by high content imaging, as in . n=7 wells per condition, nine tiled images per well, significance determined by two sample t-test. c) Change in the proportion of NPCs in S-phase in response to 1.5mM LiCl, while expressing indicated dSpCas9 and gRNAs. n=7 wells per condition, significance determined by two sample t-test.
    Antibodies Against Tdg, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against tdg/product/Proteintech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against tdg - by Bioz Stars, 2024-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    a) An NPC donor line heterozygous for index SNP (rs11709284) was transduced with lentivirus carrying the indicated gRNA, and dSpCas9:KRAB (CRISPRi) or dSpCas9:VP64 (CRISPRa). Cells were incubated for four days, followed by an eight hour treatment with 1.5 mM LiCl. GNL3 expression was analyzed by RT-qPCR. Data normalized to neg. control gRNA (non-targeting, dashed line). n=4 qPCR replicates, significance determined by two sample t-test. * p<0.05, ** p<0.01, *** p<0.001. b) Proportion of NPCs in S-phase expressing indicated dSpCas9 and gRNAs at baseline, analyzed by high content imaging, as in . n=7 wells per condition, nine tiled images per well, significance determined by two sample t-test. c) Change in the proportion of NPCs in S-phase in response to 1.5mM LiCl, while expressing indicated dSpCas9 and gRNAs. n=7 wells per condition, significance determined by two sample t-test.

    Journal: bioRxiv

    Article Title: Cellular genome wide association study identifies common genetic variation influencing lithium induced neural progenitor proliferation

    doi: 10.1101/2022.01.31.478307

    Figure Lengend Snippet: a) An NPC donor line heterozygous for index SNP (rs11709284) was transduced with lentivirus carrying the indicated gRNA, and dSpCas9:KRAB (CRISPRi) or dSpCas9:VP64 (CRISPRa). Cells were incubated for four days, followed by an eight hour treatment with 1.5 mM LiCl. GNL3 expression was analyzed by RT-qPCR. Data normalized to neg. control gRNA (non-targeting, dashed line). n=4 qPCR replicates, significance determined by two sample t-test. * p<0.05, ** p<0.01, *** p<0.001. b) Proportion of NPCs in S-phase expressing indicated dSpCas9 and gRNAs at baseline, analyzed by high content imaging, as in . n=7 wells per condition, nine tiled images per well, significance determined by two sample t-test. c) Change in the proportion of NPCs in S-phase in response to 1.5mM LiCl, while expressing indicated dSpCas9 and gRNAs. n=7 wells per condition, significance determined by two sample t-test.

    Article Snippet: gRNAs were selected using the GPP sgRNA Designer (Broad Institute) and cloned into CRISPRi (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP; Addgene # 71237) or CRISPRa (pLV hU6-gRNA (anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP; Addgene #66707) plasmids, which were kind gifts from the lab of Charles Gersbach.

    Techniques: Transduction, Incubation, Expressing, Quantitative RT-PCR, Imaging