66500 Search Results


taz antibody  (Proteintech)
94
Proteintech taz antibody
miR-34a-5p repressed <t>Hippo-YAP1/TAZ</t> signaling pathway. (a) RNA-Seq data showed that significant change was taken place in Hippo signaling pathway <t>after</t> <t>LEF1</t> was overexpressed. (b,c) YAP1/TAZ expression was downregulated after miR-34a-5p upregulation in Eca109 or TE1 cells compared to the control groups.
Taz Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taz antibody/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
taz antibody - by Bioz Stars, 2024-10
94/100 stars
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rabbit anti taz antibody  (Proteintech)
94
Proteintech rabbit anti taz antibody
<t>a</t> <t>YAP/TAZ</t> is required for the LPA-induced the transcription of Aurora A . RPE-1 cells were starved for 12 h and then transfected with control siRNA or YAP/TAZ siRNAs. Following serum starvation for another 48 h, cells were treated with 2 μM LPA for 18 h, and the mRNA levels were measured by qPCR. b Immunoblot analysis in control or YAP/TAZ knockdown cells using indicating antibodies. RPE-1 cells were transfected and treated as described in Fig. . The effect of serum- or LPA-induced cilia disassembly in control or YAP/TAZ knockdown cells. RPE-1 cells were transfected and treated as described in Fig. b, . d CMZ or EGTA blocks serum- and LPA- induced Aurora A activation. Ciliated RPE-1 cells were pretreated with CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. Cells were stained with anti-p-AurA (Aurora A, green), anti-Ac-tub (Ac-tubulin, red) antibodies. Scale bar: 5 μm (main image) and 1 μm (magnified region). e CMZ or EGTA blocks serum- and LPA- induced cilia disassembly. Ciliated RPE-1 cells were pretreated with Ki16425 (40 μM), CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. f A proposed model for LPA signaling in the regulation of cilia disassembly. Source data are provided as a Source Data file. Three experiments were repeated independently with similar results in b and d . Data are presented as mean ± S.D. of three independent experiments in a , , and e . n , number of cells. *** P < 0.001. Two-tailed Student’s t -test in a , two-way ANOVA test was performed followed by Dunnett’s multiple comparisons in and e .
Rabbit Anti Taz Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti taz antibody/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti taz antibody - by Bioz Stars, 2024-10
94/100 stars
  Buy from Supplier
taz  (Proteintech)
94
Proteintech taz
Downregulation of VASN gene expression in TPC and BCPAP cells inhibits the Hippo pathway and EMT pathway. YAP, <t>TAZ,</t> <t>N-cadherin,</t> E-cadherin, and Vimentin protein expression determined by western blotting. All samples were total unified total proteins by β-actin. The pictures were taken under a microscope before protein extraction.
Taz, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taz/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
taz - by Bioz Stars, 2024-10
94/100 stars
  Buy from Supplier
1 ap  (Proteintech)
94
Proteintech 1 ap
Downregulation of VASN gene expression in TPC and BCPAP cells inhibits the Hippo pathway and EMT pathway. YAP, <t>TAZ,</t> <t>N-cadherin,</t> E-cadherin, and Vimentin protein expression determined by western blotting. All samples were total unified total proteins by β-actin. The pictures were taken under a microscope before protein extraction.
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 ap/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1 ap - by Bioz Stars, 2024-10
94/100 stars
  Buy from Supplier
23306 1 ap  (Proteintech)
94
Proteintech 23306 1 ap
Downregulation of VASN gene expression in TPC and BCPAP cells inhibits the Hippo pathway and EMT pathway. YAP, <t>TAZ,</t> <t>N-cadherin,</t> E-cadherin, and Vimentin protein expression determined by western blotting. All samples were total unified total proteins by β-actin. The pictures were taken under a microscope before protein extraction.
23306 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/23306 1 ap/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
23306 1 ap - by Bioz Stars, 2024-10
94/100 stars
  Buy from Supplier

Image Search Results


miR-34a-5p repressed Hippo-YAP1/TAZ signaling pathway. (a) RNA-Seq data showed that significant change was taken place in Hippo signaling pathway after LEF1 was overexpressed. (b,c) YAP1/TAZ expression was downregulated after miR-34a-5p upregulation in Eca109 or TE1 cells compared to the control groups.

Journal: Journal of Cancer

Article Title: MiR-34a-5p Inhibits Proliferation, Migration, Invasion and Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma by Targeting LEF1 and Inactivation of the Hippo-YAP1/TAZ Signaling Pathway

doi: 10.7150/jca.39861

Figure Lengend Snippet: miR-34a-5p repressed Hippo-YAP1/TAZ signaling pathway. (a) RNA-Seq data showed that significant change was taken place in Hippo signaling pathway after LEF1 was overexpressed. (b,c) YAP1/TAZ expression was downregulated after miR-34a-5p upregulation in Eca109 or TE1 cells compared to the control groups.

Article Snippet: Protein extracts were subjected to SDS-PAGE and analyzed using the following primary antibodies: LEF1 antibody (Abcam, ab137872), YAP1 antibody (Abcam, ab52771), TAZ antibody (Proteintech, 23306-1-AP), E-cadherin antibody(Abcam, ab40772), N-cadherin antibody (Abcam, ab18203) and GADPH antibody (Abcam, ab8245).

Techniques: RNA Sequencing Assay, Expressing

a YAP/TAZ is required for the LPA-induced the transcription of Aurora A . RPE-1 cells were starved for 12 h and then transfected with control siRNA or YAP/TAZ siRNAs. Following serum starvation for another 48 h, cells were treated with 2 μM LPA for 18 h, and the mRNA levels were measured by qPCR. b Immunoblot analysis in control or YAP/TAZ knockdown cells using indicating antibodies. RPE-1 cells were transfected and treated as described in Fig. . The effect of serum- or LPA-induced cilia disassembly in control or YAP/TAZ knockdown cells. RPE-1 cells were transfected and treated as described in Fig. b, . d CMZ or EGTA blocks serum- and LPA- induced Aurora A activation. Ciliated RPE-1 cells were pretreated with CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. Cells were stained with anti-p-AurA (Aurora A, green), anti-Ac-tub (Ac-tubulin, red) antibodies. Scale bar: 5 μm (main image) and 1 μm (magnified region). e CMZ or EGTA blocks serum- and LPA- induced cilia disassembly. Ciliated RPE-1 cells were pretreated with Ki16425 (40 μM), CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. f A proposed model for LPA signaling in the regulation of cilia disassembly. Source data are provided as a Source Data file. Three experiments were repeated independently with similar results in b and d . Data are presented as mean ± S.D. of three independent experiments in a , , and e . n , number of cells. *** P < 0.001. Two-tailed Student’s t -test in a , two-way ANOVA test was performed followed by Dunnett’s multiple comparisons in and e .

Journal: Nature Communications

Article Title: LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis

doi: 10.1038/s41467-021-20986-y

Figure Lengend Snippet: a YAP/TAZ is required for the LPA-induced the transcription of Aurora A . RPE-1 cells were starved for 12 h and then transfected with control siRNA or YAP/TAZ siRNAs. Following serum starvation for another 48 h, cells were treated with 2 μM LPA for 18 h, and the mRNA levels were measured by qPCR. b Immunoblot analysis in control or YAP/TAZ knockdown cells using indicating antibodies. RPE-1 cells were transfected and treated as described in Fig. . The effect of serum- or LPA-induced cilia disassembly in control or YAP/TAZ knockdown cells. RPE-1 cells were transfected and treated as described in Fig. b, . d CMZ or EGTA blocks serum- and LPA- induced Aurora A activation. Ciliated RPE-1 cells were pretreated with CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. Cells were stained with anti-p-AurA (Aurora A, green), anti-Ac-tub (Ac-tubulin, red) antibodies. Scale bar: 5 μm (main image) and 1 μm (magnified region). e CMZ or EGTA blocks serum- and LPA- induced cilia disassembly. Ciliated RPE-1 cells were pretreated with Ki16425 (40 μM), CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. f A proposed model for LPA signaling in the regulation of cilia disassembly. Source data are provided as a Source Data file. Three experiments were repeated independently with similar results in b and d . Data are presented as mean ± S.D. of three independent experiments in a , , and e . n , number of cells. *** P < 0.001. Two-tailed Student’s t -test in a , two-way ANOVA test was performed followed by Dunnett’s multiple comparisons in and e .

Article Snippet: Antibodies used in this study included mouse anti-Ac-tubulin antibody (1:800, T6793, Sigma), rabbit anti-γ-Tubulin antibody (1:600, T6557, Sigma), mouse anti-LPAR1 antibody (1:100, sc-515665, Santa), mouse anti-α-Tubulin (1:5000, T5168, Sigma), mouse anti-Flag antibody (1:1000, F3165, Sigma), mouse anti-Gα 12 antibody (1:200, sc-515445, Santa), mouse anti-Gα 13 antibody (1:200, sc-293424, Santa), mouse anti-Gα q antibody (1:200, sc-136181, Santa), mouse anti-Gα 11 antibody (1:200, sc-390382, Santa), mouse anti-YAP antibody (1:1000, sc-101199, Santa), rabbit anti-TAZ antibody (1:4000, 66500-1-Ig, Proteintech), rabbit anti-Aurora A antibody (1:1000, 4718s, Cell Signaling Technologies), rabbit anti-HDAC6 antibody (1:1000, 7558T, Cell Signaling Technologies), rabbit anti-ARL13B antibody (1:400, 17711-1-AP, Proteintech), mouse anti-BrdU antibody (1:250, 11-286-C100, Exbio), rabbit anti-Pax6 antibody (1:500, PRB-278P, Covance), rat anti-Tbr2 antibody (1:500, 14-4875-82, Thermo Fisher Scientific), rabbit anti-Satb2 antibody (1:500, ab92446, Abcam), mouse anti-Tbr1 antibody (1:250, 66564-1-Ig, Proteintech), rabbit anti-Ctip2 antibody (1:250, ab28448, Abcam), rabbit anti-p-H3(Ser10) antibody (1:500, 9701s, Cell Signaling Technologies), rabbit anti-Phospho-Aurora A (Thr288) antibody (1:100, MA5-14904, Thermo Fisher Scientific), goat anti-Sox2 antibody (1:300, Sc-17320, Santa), rabbit anti-Ki67 antibody (1:500, 9129T, Cell Signaling Technologies), rabbit anti-cleaved Caspase3 antibody (1:400, 9664, Cell Signaling Technologies), mouse anti-Flag-M2 FITC (1:8000, F4049, Sigma), Goat anti-Mouse Alexa Fluor 488 (1:500, A11029, Thermo Fisher Scientific), Goat anti-Mouse Alexa Fluor 546 (1:500, A11030, Thermo Fisher Scientific), Goat anti-Mouse Alexa Fluor 647 (1:500, A21235, Thermo Fisher Scientific, lot: 2088736), Goat anti-Rabbit Alexa Fluor 488 (1:500, A11034, Thermo Fisher Scientific), Goat anti-Rabbit Alexa Fluor 546 (1:500, A11035, Thermo Fisher Scientific), Goat anti-Rabbit Alexa Fluor 647 (1:500, A21245, Thermo Fisher Scientific), Goat anti-Rat Alexa Fluor 555 (1:500, A21434, Thermo Fisher Scientific), Goat anti-Rat Alexa Fluor 488 (1:500, A11006, Thermo Fisher Scientific), Donkey anti-Goat Alexa Fluor 647 (1:500, A21447, Thermo Fisher Scientific).

Techniques: Transfection, Western Blot, Activation Assay, Staining, Two Tailed Test

a , b VZ/SVZ cells in Lpar1 − /− mice exhibit a lower mitotic index. a Representative images of mitotic cells stained with phosphorylated histone 3 (p-H3, green) and DNA (blue) in E14.5 Lpar1 +/+ and Lpar1 − /− mice cortices. Scale bar, 20 μm. b The percentage of p-H3-positive cells from VZ/SVZ cells in a , n = 8 sections from four mice. c Representative images of E14.5 Lpar1 +/+ and Lpar1 − /− cortices subjected to dual pulse-chase labeling of BrdU (green) and EdU (red). The pulse-chase timing is shown on the top. Scale bar, 20 μm. d Quantification of the ratio of EdU + cells in VZ/SVZ (top) and the ratio of BrdU + EdU + to BrdU + cells in VZ/SVZ (down), n = 8 sections from four mice. e Lpar1 − /− RG cells possess longer cilia than Lpar1 +/+ . Lpar1 +/+ and Lpar1 − /− cortices section at P0 were stained with cilia marker (ARL13B, green) and DNA (blue). Scale bar, 5 μm (main image) and 1 μm (magnified region). f Quantification of the cilium length in e , Lpar1 +/+ ː n = 179 cilia from four mice, Lpar1 − /− ː n = 149 cilia from four mice. g , h Quantification of cilium length ( g ) or percentage of Ki67 + cells ( h ) in Sox2-positive RG cells. Isolated cells from Lpar1 +/+ cerebral cortex were treated with 2 μM tubacin or DMSO for 72 h, and isolated cells from Lpar1 − /− cerebral cortex were infected with lentivirus expressed GFP or GFP-Lpar1 with or without 2 μM tubacin treatment for 72 h. g From left to right, n = 95, 164, 103, 111 cilia from four experiments. h n = 10 mice from four experiments. i qPCR analyzed the transcriptional levels of Aurora A , Ctgf , Yap and Taz in E14.5 Lpar1 +/+ and Lpar1 − /− mice cortices. j Representative images of E14.5 Lpar1 +/+ and Lpar1 − /− cortices stained with YAP (green) and PAX6 (red). Scale bars, 20 μm (main image) and 5 μm (magnified region). k Quantification of the percentage of PAX6 + cells with nuclear YAP in VZ. Source data are provided as a Source Data file. Four experiments were repeated independently with similar results in a , c , e , and j . Data are presented as mean ± S.D. in b , d , f , g , h , i , and k . * P < 0.05, ** P < 0.01, *** P < 0.001. Two-tailed Student’s t -test in b , d , f , i , and k ; One-way ANOVA test was performed followed by Bonferroni’s multiple comparisons in g and h .

Journal: Nature Communications

Article Title: LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis

doi: 10.1038/s41467-021-20986-y

Figure Lengend Snippet: a , b VZ/SVZ cells in Lpar1 − /− mice exhibit a lower mitotic index. a Representative images of mitotic cells stained with phosphorylated histone 3 (p-H3, green) and DNA (blue) in E14.5 Lpar1 +/+ and Lpar1 − /− mice cortices. Scale bar, 20 μm. b The percentage of p-H3-positive cells from VZ/SVZ cells in a , n = 8 sections from four mice. c Representative images of E14.5 Lpar1 +/+ and Lpar1 − /− cortices subjected to dual pulse-chase labeling of BrdU (green) and EdU (red). The pulse-chase timing is shown on the top. Scale bar, 20 μm. d Quantification of the ratio of EdU + cells in VZ/SVZ (top) and the ratio of BrdU + EdU + to BrdU + cells in VZ/SVZ (down), n = 8 sections from four mice. e Lpar1 − /− RG cells possess longer cilia than Lpar1 +/+ . Lpar1 +/+ and Lpar1 − /− cortices section at P0 were stained with cilia marker (ARL13B, green) and DNA (blue). Scale bar, 5 μm (main image) and 1 μm (magnified region). f Quantification of the cilium length in e , Lpar1 +/+ ː n = 179 cilia from four mice, Lpar1 − /− ː n = 149 cilia from four mice. g , h Quantification of cilium length ( g ) or percentage of Ki67 + cells ( h ) in Sox2-positive RG cells. Isolated cells from Lpar1 +/+ cerebral cortex were treated with 2 μM tubacin or DMSO for 72 h, and isolated cells from Lpar1 − /− cerebral cortex were infected with lentivirus expressed GFP or GFP-Lpar1 with or without 2 μM tubacin treatment for 72 h. g From left to right, n = 95, 164, 103, 111 cilia from four experiments. h n = 10 mice from four experiments. i qPCR analyzed the transcriptional levels of Aurora A , Ctgf , Yap and Taz in E14.5 Lpar1 +/+ and Lpar1 − /− mice cortices. j Representative images of E14.5 Lpar1 +/+ and Lpar1 − /− cortices stained with YAP (green) and PAX6 (red). Scale bars, 20 μm (main image) and 5 μm (magnified region). k Quantification of the percentage of PAX6 + cells with nuclear YAP in VZ. Source data are provided as a Source Data file. Four experiments were repeated independently with similar results in a , c , e , and j . Data are presented as mean ± S.D. in b , d , f , g , h , i , and k . * P < 0.05, ** P < 0.01, *** P < 0.001. Two-tailed Student’s t -test in b , d , f , i , and k ; One-way ANOVA test was performed followed by Bonferroni’s multiple comparisons in g and h .

Article Snippet: Antibodies used in this study included mouse anti-Ac-tubulin antibody (1:800, T6793, Sigma), rabbit anti-γ-Tubulin antibody (1:600, T6557, Sigma), mouse anti-LPAR1 antibody (1:100, sc-515665, Santa), mouse anti-α-Tubulin (1:5000, T5168, Sigma), mouse anti-Flag antibody (1:1000, F3165, Sigma), mouse anti-Gα 12 antibody (1:200, sc-515445, Santa), mouse anti-Gα 13 antibody (1:200, sc-293424, Santa), mouse anti-Gα q antibody (1:200, sc-136181, Santa), mouse anti-Gα 11 antibody (1:200, sc-390382, Santa), mouse anti-YAP antibody (1:1000, sc-101199, Santa), rabbit anti-TAZ antibody (1:4000, 66500-1-Ig, Proteintech), rabbit anti-Aurora A antibody (1:1000, 4718s, Cell Signaling Technologies), rabbit anti-HDAC6 antibody (1:1000, 7558T, Cell Signaling Technologies), rabbit anti-ARL13B antibody (1:400, 17711-1-AP, Proteintech), mouse anti-BrdU antibody (1:250, 11-286-C100, Exbio), rabbit anti-Pax6 antibody (1:500, PRB-278P, Covance), rat anti-Tbr2 antibody (1:500, 14-4875-82, Thermo Fisher Scientific), rabbit anti-Satb2 antibody (1:500, ab92446, Abcam), mouse anti-Tbr1 antibody (1:250, 66564-1-Ig, Proteintech), rabbit anti-Ctip2 antibody (1:250, ab28448, Abcam), rabbit anti-p-H3(Ser10) antibody (1:500, 9701s, Cell Signaling Technologies), rabbit anti-Phospho-Aurora A (Thr288) antibody (1:100, MA5-14904, Thermo Fisher Scientific), goat anti-Sox2 antibody (1:300, Sc-17320, Santa), rabbit anti-Ki67 antibody (1:500, 9129T, Cell Signaling Technologies), rabbit anti-cleaved Caspase3 antibody (1:400, 9664, Cell Signaling Technologies), mouse anti-Flag-M2 FITC (1:8000, F4049, Sigma), Goat anti-Mouse Alexa Fluor 488 (1:500, A11029, Thermo Fisher Scientific), Goat anti-Mouse Alexa Fluor 546 (1:500, A11030, Thermo Fisher Scientific), Goat anti-Mouse Alexa Fluor 647 (1:500, A21235, Thermo Fisher Scientific, lot: 2088736), Goat anti-Rabbit Alexa Fluor 488 (1:500, A11034, Thermo Fisher Scientific), Goat anti-Rabbit Alexa Fluor 546 (1:500, A11035, Thermo Fisher Scientific), Goat anti-Rabbit Alexa Fluor 647 (1:500, A21245, Thermo Fisher Scientific), Goat anti-Rat Alexa Fluor 555 (1:500, A21434, Thermo Fisher Scientific), Goat anti-Rat Alexa Fluor 488 (1:500, A11006, Thermo Fisher Scientific), Donkey anti-Goat Alexa Fluor 647 (1:500, A21447, Thermo Fisher Scientific).

Techniques: Staining, Pulse Chase, Labeling, Marker, Isolation, Infection, Two Tailed Test

Downregulation of VASN gene expression in TPC and BCPAP cells inhibits the Hippo pathway and EMT pathway. YAP, TAZ, N-cadherin, E-cadherin, and Vimentin protein expression determined by western blotting. All samples were total unified total proteins by β-actin. The pictures were taken under a microscope before protein extraction.

Journal: American Journal of Translational Research

Article Title: VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro

doi:

Figure Lengend Snippet: Downregulation of VASN gene expression in TPC and BCPAP cells inhibits the Hippo pathway and EMT pathway. YAP, TAZ, N-cadherin, E-cadherin, and Vimentin protein expression determined by western blotting. All samples were total unified total proteins by β-actin. The pictures were taken under a microscope before protein extraction.

Article Snippet: The membranes were blotted with 5% non-fat milk for in TBST 2 hours at room temperature, then, washed 3 times by TBST and probed with mouse anti N-cadherin (cat no. 66219-1-Ig; Proteintech, Inc; 1:2,000 dilution), vimentin (cat no. 60330-1-Ig; Proteintech, Inc; 1:2,000 dilution), E-cadherin (cat no. 60335-1-Ig; Proteintech, Inc; 1:2,000 dilution), TAZ (cat no. 66500-1-Ig; Proteintech, Inc; 1:1000 dilution), YAP1 (cat no. 66900-1-Ig; Proteintech, Inc; 1:1000 dilution), Rabbit-VASN (cat no. YN2042; ImmunoWay Biotechnology, lnc; 1:1000 dilution) and mouse-β-actin (cat no. 66009-1-Ig; Proteintech, Inc; 1:2,000 dilution) according to the manufacturer’s protocol overnight at 4°C.

Techniques: Expressing, Western Blot, Microscopy, Protein Extraction