66243 Search Results


93
Proteintech anti ca9 antibody
<t>CA9,</t> CXCL9, and GIMAP7 regulate the immune infiltration of tumour microenvironment in PAAD. ( A – C ) The overall survival rate of the patients with PAAD were computed with the GEPIA web tool. ( D – F ) The Timer web tool was used to determine the association between the expression levels of CA9 ( D ), CXCL9 ( E ) and GIMAP7 ( F ) with the infiltration level of immune cells in PAAD samples.
Anti Ca9 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ca9 antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
anti ca9 antibody - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech carbonic anhydrase 9
Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic <t>anhydrase</t> <t>9</t> <t>(CA9).</t> (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.
Carbonic Anhydrase 9, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/carbonic anhydrase 9/product/Proteintech
Average 93 stars, based on 1 article reviews
carbonic anhydrase 9 - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech anti ca9 rabbit polyclonal antibody
Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic <t>anhydrase</t> <t>9</t> <t>(CA9).</t> (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.
Anti Ca9 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ca9 rabbit polyclonal antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
anti ca9 rabbit polyclonal antibody - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech ca9
Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic <t>anhydrase</t> <t>9</t> <t>(CA9).</t> (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.
Ca9, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca9/product/Proteintech
Average 93 stars, based on 1 article reviews
ca9 - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech anti caix
Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic <t>anhydrase</t> <t>9</t> <t>(CA9).</t> (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.
Anti Caix, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti caix/product/Proteintech
Average 93 stars, based on 1 article reviews
anti caix - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

Image Search Results


CA9, CXCL9, and GIMAP7 regulate the immune infiltration of tumour microenvironment in PAAD. ( A – C ) The overall survival rate of the patients with PAAD were computed with the GEPIA web tool. ( D – F ) The Timer web tool was used to determine the association between the expression levels of CA9 ( D ), CXCL9 ( E ) and GIMAP7 ( F ) with the infiltration level of immune cells in PAAD samples.

Journal: Aging (Albany NY)

Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma

doi: 10.18632/aging.102931

Figure Lengend Snippet: CA9, CXCL9, and GIMAP7 regulate the immune infiltration of tumour microenvironment in PAAD. ( A – C ) The overall survival rate of the patients with PAAD were computed with the GEPIA web tool. ( D – F ) The Timer web tool was used to determine the association between the expression levels of CA9 ( D ), CXCL9 ( E ) and GIMAP7 ( F ) with the infiltration level of immune cells in PAAD samples.

Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400), anti-CA9 antibody (Proteintech, Cat# 11071-1-AP, 1:200), and anti-FOXO1 antibody (Proteintech, Cat# 18592-1-AP, 1:1000).

Techniques: Expressing

FOXOs regulate the immune infiltration of tumor microenvironment in PAAD. ( A – C ) The Timer web tool was used to determine the association between the expression levels of FOXO1 ( A ), FOXO3 ( B ), and FOXO4 ( C ) with the infiltration level of immune cells in PAAD samples. ( D – F ) The GEPIA web tool was used to determine the correlation between the mRNA expression levels of CA9 ( D ), CXCL9 ( E ), and GIMAP7 ( F ) with FoxOs in PAAD samples, respectively.

Journal: Aging (Albany NY)

Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma

doi: 10.18632/aging.102931

Figure Lengend Snippet: FOXOs regulate the immune infiltration of tumor microenvironment in PAAD. ( A – C ) The Timer web tool was used to determine the association between the expression levels of FOXO1 ( A ), FOXO3 ( B ), and FOXO4 ( C ) with the infiltration level of immune cells in PAAD samples. ( D – F ) The GEPIA web tool was used to determine the correlation between the mRNA expression levels of CA9 ( D ), CXCL9 ( E ), and GIMAP7 ( F ) with FoxOs in PAAD samples, respectively.

Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400), anti-CA9 antibody (Proteintech, Cat# 11071-1-AP, 1:200), and anti-FOXO1 antibody (Proteintech, Cat# 18592-1-AP, 1:1000).

Techniques: Expressing

CA9, CXCL9, and GIMAP7 regulate the expression level of FOXO1 in PAAD. PANC-1 and SW 1990 cells were transfected with indicated siRNA. Then, 24 hrs post-transfection, cells were harvested for RT-qPCR. The data shown were the mean values ± SD from three replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. After 48 h post-transfection, cells were harvested for Western Blot analysis. ( A – C ) PANC-1 and SW 1990 cells transfected with Si-CA9 ( A ), Si-CXCL9 ( B ) and Si-GIMAP7 ( C ) were harvested for RT-qPCR. ( D – F ) PANC-1 and SW 1990 cells transfected with Si-CA9 ( D ), Si-CXCL9 ( E ) and Si-GIMAP7 ( F ) were harvested for RT-qPCR and western blotting. ( G ) PANC-1 cells transfected with Si-CA9 ( C ), Si-CXCL9 ( E ), or Si-GIMAP7 ( G ) were harvested for RT-qPCR.

Journal: Aging (Albany NY)

Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma

doi: 10.18632/aging.102931

Figure Lengend Snippet: CA9, CXCL9, and GIMAP7 regulate the expression level of FOXO1 in PAAD. PANC-1 and SW 1990 cells were transfected with indicated siRNA. Then, 24 hrs post-transfection, cells were harvested for RT-qPCR. The data shown were the mean values ± SD from three replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. After 48 h post-transfection, cells were harvested for Western Blot analysis. ( A – C ) PANC-1 and SW 1990 cells transfected with Si-CA9 ( A ), Si-CXCL9 ( B ) and Si-GIMAP7 ( C ) were harvested for RT-qPCR. ( D – F ) PANC-1 and SW 1990 cells transfected with Si-CA9 ( D ), Si-CXCL9 ( E ) and Si-GIMAP7 ( F ) were harvested for RT-qPCR and western blotting. ( G ) PANC-1 cells transfected with Si-CA9 ( C ), Si-CXCL9 ( E ), or Si-GIMAP7 ( G ) were harvested for RT-qPCR.

Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400), anti-CA9 antibody (Proteintech, Cat# 11071-1-AP, 1:200), and anti-FOXO1 antibody (Proteintech, Cat# 18592-1-AP, 1:1000).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

CA9, CXCL9, and GIMAP7 are correlated with FOXO1 in PAAD patient specimens respectively. ( A – C ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and CA9 respectively. The typical image of FOXO1 and CA9 was shown in ( A ), the IHC scores of FOXO1 and CA9 was shown in ( B ) and the correlation of these two proteins was shown in ( C ). ( D – F ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and CXCL9, respectively. The typical image of FOXO1 and CXCL9 was shown in ( D ), the IHC scores of FOXO1 and CXCL9 was shown in ( E ) and the correlation of these two proteins was shown in ( F ). ( G – I ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and GIMAP7, respectively. The typical image of FOXO1 and GIMAP7 was shown in ( G ), the IHC scores of FOXO1 and GIMAP7 was shown in ( H ), and the correlation of these two proteins was shown in ( I ). The scale in A , D , and G represents 1mm or 50um, respectively.

Journal: Aging (Albany NY)

Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma

doi: 10.18632/aging.102931

Figure Lengend Snippet: CA9, CXCL9, and GIMAP7 are correlated with FOXO1 in PAAD patient specimens respectively. ( A – C ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and CA9 respectively. The typical image of FOXO1 and CA9 was shown in ( A ), the IHC scores of FOXO1 and CA9 was shown in ( B ) and the correlation of these two proteins was shown in ( C ). ( D – F ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and CXCL9, respectively. The typical image of FOXO1 and CXCL9 was shown in ( D ), the IHC scores of FOXO1 and CXCL9 was shown in ( E ) and the correlation of these two proteins was shown in ( F ). ( G – I ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and GIMAP7, respectively. The typical image of FOXO1 and GIMAP7 was shown in ( G ), the IHC scores of FOXO1 and GIMAP7 was shown in ( H ), and the correlation of these two proteins was shown in ( I ). The scale in A , D , and G represents 1mm or 50um, respectively.

Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400), anti-CA9 antibody (Proteintech, Cat# 11071-1-AP, 1:200), and anti-FOXO1 antibody (Proteintech, Cat# 18592-1-AP, 1:1000).

Techniques: Microarray, Staining

Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic anhydrase 9 (CA9). (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.

Journal: Bioengineered

Article Title: A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma

doi: 10.1080/21655979.2022.2073943

Figure Lengend Snippet: Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic anhydrase 9 (CA9). (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.

Article Snippet: Primary antibodies against HIF1α (1:100; Cat No. 20960-1-AP; Proteintech, Wuhan, China), carbonic anhydrase 9 (CA9; 1:200; Cat No. 11071-1-AP; Proteintech, Wuhan, China), CFHR3 (1:100; Cat No. 16583-1-AP; Proteintech, Wuhan, China), and proliferating cell nuclear antigen (PCNA; 1:200; Cat No. 10205-2-AP; Proteintech, Wuhan, China) were incubated overnight at 4°C.

Techniques: In Vitro, Immunohistochemical staining, Staining, Expressing, Western Blot, Cell Culture, Negative Control, Transfection, Plasmid Preparation

Exploration of the biological functions of CFHR3 in HCC cells in vivo . (a, b) The proliferation of HepG2 cells with CFHR3 overexpression and NC cells in vivo . (c) Expression levels of HIF1α, CA9, CFHR3, and proliferating cell nuclear antigen (PCNA) in tumor tissues derived from HepG2 cells with CFHR3 overexpression and NC cells. (d, e) Metastatic foci in the lungs from mice injected with SMMC-7721 cells with CFHR3 overexpression and NC cells. ** P < 0.01.

Journal: Bioengineered

Article Title: A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma

doi: 10.1080/21655979.2022.2073943

Figure Lengend Snippet: Exploration of the biological functions of CFHR3 in HCC cells in vivo . (a, b) The proliferation of HepG2 cells with CFHR3 overexpression and NC cells in vivo . (c) Expression levels of HIF1α, CA9, CFHR3, and proliferating cell nuclear antigen (PCNA) in tumor tissues derived from HepG2 cells with CFHR3 overexpression and NC cells. (d, e) Metastatic foci in the lungs from mice injected with SMMC-7721 cells with CFHR3 overexpression and NC cells. ** P < 0.01.

Article Snippet: Primary antibodies against HIF1α (1:100; Cat No. 20960-1-AP; Proteintech, Wuhan, China), carbonic anhydrase 9 (CA9; 1:200; Cat No. 11071-1-AP; Proteintech, Wuhan, China), CFHR3 (1:100; Cat No. 16583-1-AP; Proteintech, Wuhan, China), and proliferating cell nuclear antigen (PCNA; 1:200; Cat No. 10205-2-AP; Proteintech, Wuhan, China) were incubated overnight at 4°C.

Techniques: In Vivo, Over Expression, Expressing, Derivative Assay, Injection