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Image Search Results

Journal: Aging (Albany NY)
Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma
doi: 10.18632/aging.102931
Figure Lengend Snippet: CA9, CXCL9, and GIMAP7 regulate the immune infiltration of tumour microenvironment in PAAD. ( A – C ) The overall survival rate of the patients with PAAD were computed with the GEPIA web tool. ( D – F ) The Timer web tool was used to determine the association between the expression levels of CA9 ( D ), CXCL9 ( E ) and GIMAP7 ( F ) with the infiltration level of immune cells in PAAD samples.
Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400),
Techniques: Expressing

Journal: Aging (Albany NY)
Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma
doi: 10.18632/aging.102931
Figure Lengend Snippet: FOXOs regulate the immune infiltration of tumor microenvironment in PAAD. ( A – C ) The Timer web tool was used to determine the association between the expression levels of FOXO1 ( A ), FOXO3 ( B ), and FOXO4 ( C ) with the infiltration level of immune cells in PAAD samples. ( D – F ) The GEPIA web tool was used to determine the correlation between the mRNA expression levels of CA9 ( D ), CXCL9 ( E ), and GIMAP7 ( F ) with FoxOs in PAAD samples, respectively.
Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400),
Techniques: Expressing

Journal: Aging (Albany NY)
Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma
doi: 10.18632/aging.102931
Figure Lengend Snippet: CA9, CXCL9, and GIMAP7 regulate the expression level of FOXO1 in PAAD. PANC-1 and SW 1990 cells were transfected with indicated siRNA. Then, 24 hrs post-transfection, cells were harvested for RT-qPCR. The data shown were the mean values ± SD from three replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. After 48 h post-transfection, cells were harvested for Western Blot analysis. ( A – C ) PANC-1 and SW 1990 cells transfected with Si-CA9 ( A ), Si-CXCL9 ( B ) and Si-GIMAP7 ( C ) were harvested for RT-qPCR. ( D – F ) PANC-1 and SW 1990 cells transfected with Si-CA9 ( D ), Si-CXCL9 ( E ) and Si-GIMAP7 ( F ) were harvested for RT-qPCR and western blotting. ( G ) PANC-1 cells transfected with Si-CA9 ( C ), Si-CXCL9 ( E ), or Si-GIMAP7 ( G ) were harvested for RT-qPCR.
Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400),
Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

Journal: Aging (Albany NY)
Article Title: Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma
doi: 10.18632/aging.102931
Figure Lengend Snippet: CA9, CXCL9, and GIMAP7 are correlated with FOXO1 in PAAD patient specimens respectively. ( A – C ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and CA9 respectively. The typical image of FOXO1 and CA9 was shown in ( A ), the IHC scores of FOXO1 and CA9 was shown in ( B ) and the correlation of these two proteins was shown in ( C ). ( D – F ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and CXCL9, respectively. The typical image of FOXO1 and CXCL9 was shown in ( D ), the IHC scores of FOXO1 and CXCL9 was shown in ( E ) and the correlation of these two proteins was shown in ( F ). ( G – I ) the tissue microarray of PAAD patients (n=31) was stained with FOXO1 and GIMAP7, respectively. The typical image of FOXO1 and GIMAP7 was shown in ( G ), the IHC scores of FOXO1 and GIMAP7 was shown in ( H ), and the correlation of these two proteins was shown in ( I ). The scale in A , D , and G represents 1mm or 50um, respectively.
Article Snippet: To study altered protein expression, an IHC analysis was performed on tissue microarray (TMA) slides purchased from Outdo Biobank (Cat No. XT14-029) (Shanghai, China), using GIMAP7 antibody (Sigma, Cat# HPA020268,1:50), anti-CXCL9 antibody (Abcam, Cat# ab9720, 1:400),
Techniques: Microarray, Staining

Journal: Bioengineered
Article Title: A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma
doi: 10.1080/21655979.2022.2073943
Figure Lengend Snippet: Exploration of the biological functions of CFHR3 in HCC cells in vitro . (a) Immunohistochemical staining demonstrates that CFHR3 expression is decreased in HCC tissues with high expression levels of hypoxia inducible factor 1 subunit alpha (HIF1A) and carbonic anhydrase 9 (CA9). (b-c) Western blot was used to detect the expression of CFHR3 in HCC cells in the blank control group (BC, without any treatment; cultured under normoxia and hypoxia), in the negative control group (NC, transfected with vector; cultured under normoxia and hypoxia) and in the CFHR3 overexpressing group (CFHR3-OE; cultured under hypoxia). (d-e) 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect the effects of CFHR3 on HCC cell proliferation under hypoxia. (f-g) Colony formation assays were used to detect the effects of CFHR3 on HCC cell colony formation under hypoxia. (h-i) Transwell assays were used to detect the effects of CFHR3 on HCC cell invasiveness under hypoxia. ** P < 0.01.
Article Snippet: Primary antibodies against HIF1α (1:100; Cat No. 20960-1-AP; Proteintech, Wuhan, China),
Techniques: In Vitro, Immunohistochemical staining, Staining, Expressing, Western Blot, Cell Culture, Negative Control, Transfection, Plasmid Preparation

Journal: Bioengineered
Article Title: A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma
doi: 10.1080/21655979.2022.2073943
Figure Lengend Snippet: Exploration of the biological functions of CFHR3 in HCC cells in vivo . (a, b) The proliferation of HepG2 cells with CFHR3 overexpression and NC cells in vivo . (c) Expression levels of HIF1α, CA9, CFHR3, and proliferating cell nuclear antigen (PCNA) in tumor tissues derived from HepG2 cells with CFHR3 overexpression and NC cells. (d, e) Metastatic foci in the lungs from mice injected with SMMC-7721 cells with CFHR3 overexpression and NC cells. ** P < 0.01.
Article Snippet: Primary antibodies against HIF1α (1:100; Cat No. 20960-1-AP; Proteintech, Wuhan, China),
Techniques: In Vivo, Over Expression, Expressing, Derivative Assay, Injection