Journal: Experimental and Therapeutic Medicine

Article Title: GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial-mesenchymal transition and metastasis through regulation of SLUG

doi: 10.3892/etm.2018.6826

Figure Lengend Snippet: GATA4 facilitates epithelial-mesenchymal transition in nasopharyngeal cancer cells. 5-8F cells were transfected with an empty vector, FLAG-GATA4, SCR or siGATA4. (A) Western blotting was used to detect the protein levels and (B) reverse transcription-quantitative polymerase chain reaction analysis was used to detect and quantify the mRNA expression levels of E-cadherin, α-catenin, N-cadherin and vimentin. *P<0.05. FLAG-GATA4, vector overexpressing GATA4; si, small interfering RNA; SCR, scramble siRNA; siGATA4, siRNA against GATA4.

Article Snippet: The antibodies used were as follows: Rabbit anti-GATA4 (1:2,000; cat, 19530-1-AP; Proteintech Group, Inc., Chicago, IL, USA), rabbit anti-TWIST (1:1,000; cat: 25465-1-AP; Proteintech Group, Inc.), rabbit anti-a-catenin (1:3,000; cat, 12831-1-AP; Proteintech Group, Inc.), rabbit antibodies from the EMT kit (1:2,000; cat, 9783; Cell Signaling Technology, Inc., Danvers, MA, USA) and mouse anit-β-actin (1:3,000; cat, 6276; Abcam, Cambridge, UK), goat anti-rabbit (HRP-conjugated; 1:5,000; cat, ab205718; Abcam) and goat anti-mouse (HRP-conjugated; 1:3,000; cat, ab205719; Abcam).

Techniques: Transfection, Plasmid Preparation, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Small Interfering RNA

Journal: Oncotarget

Article Title: YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo

doi: 10.18632/oncotarget.13188

Figure Lengend Snippet: The wound healing assay was used to evaluate the migration properties of BGC-823 GC cells ( A , G ) and GES-1 cells ( B , H ). Cells were photographed 0, 24, 48, and 72 h after wounding (magnification×100). The Transwell assay was used to evaluate the migration properties of BGC-823 GC cells ( C , I ) and GES-1 cells ( D , J ) (magnification: ×200). The Transwell assay was used to evaluate the invasion properties of BGC-823 GC cells ( E , K ) and GES-1 cells ( F , L ). ( M ) p-ERK1/2, ERK1/2, epithelial-related protein E-cadherin, mesenchymal related protein Vimentin, α-catenin, and β-catenin expressions were determined by western blot. β-actin was used as an inner control. HOX transcript antisense RNA (HOTAIR) ( N , O ), H19 ( P , Q ), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) ( R , S ), human large tumor suppressor 2 (LATS2)-AS1-001 ( T , U ), and LATS2 ( V , W ) LncRNA expressions and YAP1 mRNA expression ( X , Y ) in BGC-823 and GES-1 cells were determined by qRT-PCR. Data are shown as mean ± SD. * P < 0.05; ** P < 0.01.

Article Snippet: The primary antibodies were: E-cadherin (1:500, Cell signaling, #3195), Vimentin (1:1000, Cell signaling, #5741), ERK1/2 (1:1000, Cell signaling; #4695), p-ERK1/2 (Thr202/Tyr204) (1:1000, Cell signaling, #9101), α-catenin (1:500, Proteintech, Catalog number: 66221-1-Ig), β-catenin (1:1000, Santa Cruz, sc-7963), and β-actin (1:1000, Bioss).

Techniques: Wound Healing Assay, Migration, Transwell Assay, Western Blot, Expressing, Quantitative RT-PCR

Journal: Development (Cambridge, England)

Article Title: Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis

doi: 10.1242/dev.055566

Figure Lengend Snippet: Perturbation of adhesion junctions in Daam1gt/gt cardiomyocytes. (A) Aberrant staining pattern of α-catenin and N-cadherin in E12.5 Daam1gt/gt cardiomyocytes. The Daam1gt/gt cardiomyocytes of the developing ventricular myocardial wall (b,e,h) have lost their uniform expression of α-catenin and N-cadherin compared with the wild-type (a,d,g) and Daam1gt/gt/Nkx2-5Cre/+ (c,f,i) cardiomyocytes. Scale bars: 5 μm. (B) Aberrant adherens junctions in E12.5 Daam1gt/gt cardiomyocytes. The adherens junction in Daam1gt/gt cardiomyocytes (b) has prominent plaques (asterisk), which are not positioned nicely alongside of the plasma membrane, as seen with adherens junctions in wild-type (a) and Daam1gt/gt/Nkx2-5Cre/+ (c) cardiomyocytes. (a) Arrow indicates a normal desmosome. Scale bars: 0.25 μm. (C) Cell misalignment in Daam1gt/gt hearts. E12.5 heart sections are stained with WGA (for membranes) and PI (for nuclei). Cardiomyocytes in developing ventricular walls (the cells within the bracket) of Daam1gt/+ (a) and Daam1gt/gt/Nkx2-5Cre/+ (c) exhibit fine alignment parallel to the epicardium (arrows), whereas Daam1gt/gt cardiomyocytes (b) manifest a randomized distribution. Scale bars: 10 μm. (D) Abnormal adhesion of cultured E12.5 Daam1gt/gt cardiomyocytes to collagen type I. Cardiomyocytes of three genotypes (a-c) were stained by α-actinin. Scale bars: 50 μm. (d) Quantification of the adhesion assay is shown in D. *Significantly different from heterozygous and rescue, P<0.05. Results are mean ± s.e.m.

Article Snippet: Antibodies used were: monoclonal antibody (mAb) against Daam1 (Abcam), which recognizes the N terminus of Daam1 protein; polyclonal antibody (pAb) against Daam1 (Proteintech), which recognizes the C terminus of Daam1 protein; mAb against α-catenin, Dvl2 and RhoA, and pAb against N-cadherin, ROCK1, ROCK2 and Cdc42 (Santa Cruz Biotechnology); pAb against phospho-JNK (Thr183/Tyr185), total-JNK and phospho-LIMK1 (Thr508) (Cell Signaling Technology); pAb against phospho-MYPT1 (Thr696); mAb again Rac1 (Upstate); and mAb against α-actin and α-actinin (Sigma).

Techniques: Staining, Expressing, Cell Culture, Cell Adhesion Assay