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  • 93
    Proteintech antibody against ube2c
    Clinicopathological characteristics of patient samples and <t> UBE2C </t> expression in NPC
    Antibody Against Ube2c, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Proteintech mouse anti human ube2c
    Clinicopathological characteristics of patient samples and <t> UBE2C </t> expression in NPC
    Mouse Anti Human Ube2c, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human ube2c/product/Proteintech
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    92
    Santa Cruz Biotechnology tge virus antibody 1 q 17
    Clinicopathological characteristics of patient samples and <t> UBE2C </t> expression in NPC
    Tge Virus Antibody 1 Q 17, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tge virus antibody 1 q 17/product/Santa Cruz Biotechnology
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    93
    Proteintech 66087 1 rrid ab 11232220
    Clinicopathological characteristics of patient samples and <t> UBE2C </t> expression in NPC
    66087 1 Rrid Ab 11232220, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Proteintech ubch10 antibody
    Primers sequences for quantitative PCR
    Ubch10 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ubch10 antibody - by Bioz Stars, 2024-06
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    Image Search Results


    Clinicopathological characteristics of patient samples and  UBE2C  expression in NPC

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: Clinicopathological characteristics of patient samples and UBE2C expression in NPC

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Expressing

    Representative photographs of high UBE2C expression in NPC samples and low UBE2C expression in non-cancerous nasopharyngeal epithelial hyperplasia (NEH). The immunohistochemical PV9000 method was used to detect UBE2C protein expression in clinical samples. Non-immune IgG was used as a negative control. The expression and location of UBE2C in cells was revealed by staining with DAB and counterstaining with hematoxylin. IRS >6.0 in NPC (cases 1 & 2) and IRS <6.0 in NEH (case 3). Original magnification for cases 1–3, ×200, for the IgG control, ×100. Scale bar = 100 μM.

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: Representative photographs of high UBE2C expression in NPC samples and low UBE2C expression in non-cancerous nasopharyngeal epithelial hyperplasia (NEH). The immunohistochemical PV9000 method was used to detect UBE2C protein expression in clinical samples. Non-immune IgG was used as a negative control. The expression and location of UBE2C in cells was revealed by staining with DAB and counterstaining with hematoxylin. IRS >6.0 in NPC (cases 1 & 2) and IRS <6.0 in NEH (case 3). Original magnification for cases 1–3, ×200, for the IgG control, ×100. Scale bar = 100 μM.

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Expressing, Immunohistochemical staining, Negative Control, Staining

    Correlation between clinicopathological characteristics and  UBE2C  protein expression in NPC

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: Correlation between clinicopathological characteristics and UBE2C protein expression in NPC

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Expressing

    UBE2C expression in variously differentiated NPC cell lines and NP-69 cells. ( A ) Well-differentiated CNE1 cells, poorly-differentiated CNE2Z cells, undifferentiated C666-1 cells and immortalized NP-69 cells were seeded in triplicate in 24-well plates for each cell line (n=3), and were harvested for the analysis of UBE2C mRNA expression by qRT-PCR; β-actin was used as a loading control. ** P <0.01 vs . NP-69, # P <0.05 vs. CNE1, Δ P <0.05 vs. CNE2Z. ( B ) Western blotting analysis the UBE2C protein expression in CNE1, CNE2Z, C666-1 and NP-69 cells. β-actin was used as a loading control.

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: UBE2C expression in variously differentiated NPC cell lines and NP-69 cells. ( A ) Well-differentiated CNE1 cells, poorly-differentiated CNE2Z cells, undifferentiated C666-1 cells and immortalized NP-69 cells were seeded in triplicate in 24-well plates for each cell line (n=3), and were harvested for the analysis of UBE2C mRNA expression by qRT-PCR; β-actin was used as a loading control. ** P <0.01 vs . NP-69, # P <0.05 vs. CNE1, Δ P <0.05 vs. CNE2Z. ( B ) Western blotting analysis the UBE2C protein expression in CNE1, CNE2Z, C666-1 and NP-69 cells. β-actin was used as a loading control.

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Representative fluorescent photographs of UBE2C protein expression in various NPC cell lines and NP-69 cells. Cells grown on glass coverslips were incubated with primary rabbit-anti human UBE2C antibody overnight; the antigenic site of UBE2C was located by TRITC-conjugated goat anti-rabbit IgG (H+L) and photographed by confocal microscopy. Scale bars = 100 μM.

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: Representative fluorescent photographs of UBE2C protein expression in various NPC cell lines and NP-69 cells. Cells grown on glass coverslips were incubated with primary rabbit-anti human UBE2C antibody overnight; the antigenic site of UBE2C was located by TRITC-conjugated goat anti-rabbit IgG (H+L) and photographed by confocal microscopy. Scale bars = 100 μM.

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Expressing, Incubation, Confocal Microscopy

    siRNA inhibited UBE2C expression in NPC cells and consequently resulted in attenuated cell proliferation. ( A ) C666-1 cells were transfected with si-UBE2C or si-Control or without siRNA (MOCK). Forty-eight hours later, UBE2C expression was assessed by PCR and immunofluorescence using TRITC-conjugated IgG (H+L). ( B ) Western blotting analysis the UBE2C protein expression in various cell lines post tranfection of siRNAs, the β-actin was used as loading controls. ( C ) Twenty-four and 48 h after transfection, CCK-8 assays were used to analyze the proliferation of various types of NPC cells. Values of optical density (OD) were obtained by the absorbance at the dual wavelengths 450/630 nM, and the results indicating the cell viability were plotted as the percentage over controls (MOCK cells). * P <0.05, ** P <0.01 vs . mock or si-Control–treated groups.

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: siRNA inhibited UBE2C expression in NPC cells and consequently resulted in attenuated cell proliferation. ( A ) C666-1 cells were transfected with si-UBE2C or si-Control or without siRNA (MOCK). Forty-eight hours later, UBE2C expression was assessed by PCR and immunofluorescence using TRITC-conjugated IgG (H+L). ( B ) Western blotting analysis the UBE2C protein expression in various cell lines post tranfection of siRNAs, the β-actin was used as loading controls. ( C ) Twenty-four and 48 h after transfection, CCK-8 assays were used to analyze the proliferation of various types of NPC cells. Values of optical density (OD) were obtained by the absorbance at the dual wavelengths 450/630 nM, and the results indicating the cell viability were plotted as the percentage over controls (MOCK cells). * P <0.05, ** P <0.01 vs . mock or si-Control–treated groups.

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Expressing, Transfection, Immunofluorescence, Western Blot, CCK-8 Assay

    Knockdown of UBE2C with siRNA arrested the cell cycle at G2-M and S phase. CNE1, CNE2Z, C666-1 and NP-69 cells were transfected with UBE2C siRNA (si-UBE2C) or si-Control or left untransfected (MOCK) for 48 h, then the cell cycle was assessed by FCM.

    Journal: BMC Cancer

    Article Title: High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

    doi: 10.1186/1471-2407-13-192

    Figure Lengend Snippet: Knockdown of UBE2C with siRNA arrested the cell cycle at G2-M and S phase. CNE1, CNE2Z, C666-1 and NP-69 cells were transfected with UBE2C siRNA (si-UBE2C) or si-Control or left untransfected (MOCK) for 48 h, then the cell cycle was assessed by FCM.

    Article Snippet: After overnight incubation with primary antibody against UBE2C (1/100) at 4°C, the antigenic sites were detected using TRITC-conjugated goat anti-rabbit IgG (1/100, Protein Tech Group, Inc., Chicago, IL, USA).

    Techniques: Transfection

    Primers sequences for quantitative PCR

    Journal: American Journal of Translational Research

    Article Title: RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma

    doi:

    Figure Lengend Snippet: Primers sequences for quantitative PCR

    Article Snippet: Then the sections were incubated with RIZ1 antibody (Abcam, UK) and UbcH10 antibody (Proteintech, USA) at 4°C overnight.

    Techniques: Sequencing

    Statistical analysis of the correlation between the expression of RIZ1 and UbcH10 in meningiomas. A: Expression of RIZ1 decreased with the increase of pathological grade and the UbcH10 expression was positively correlated with the malignancy grade in primary meningioma cells. B: Overexpression of RIZ1 decreased the UbcH10 protein level in M4 (WHO III) cells. C: RIZ1 silencing increased the UbcH10 Protein level in M1 (WHO I) cells. D: High expression of RIZ1 in the cytoplasm and nucleus of benign meningiomas (1), whereas the staining was significantly reduced in meningiomas with the increase of pathological grade (2, 3). The UbcH10 detected in cell nucleus was significantly lower in benign meningiomas (4) than that in atypical/anaplastic meningiomas (5, 6) by immunohistochemistry. E, F: Quantitative analysis of RIZ1 and UbcH10 expression by immunohistochemistry in meningiomas of different grades. G: RIZ1 and UbcH10 expression showed a positive correlation in meningioma patient samples.

    Journal: American Journal of Translational Research

    Article Title: RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma

    doi:

    Figure Lengend Snippet: Statistical analysis of the correlation between the expression of RIZ1 and UbcH10 in meningiomas. A: Expression of RIZ1 decreased with the increase of pathological grade and the UbcH10 expression was positively correlated with the malignancy grade in primary meningioma cells. B: Overexpression of RIZ1 decreased the UbcH10 protein level in M4 (WHO III) cells. C: RIZ1 silencing increased the UbcH10 Protein level in M1 (WHO I) cells. D: High expression of RIZ1 in the cytoplasm and nucleus of benign meningiomas (1), whereas the staining was significantly reduced in meningiomas with the increase of pathological grade (2, 3). The UbcH10 detected in cell nucleus was significantly lower in benign meningiomas (4) than that in atypical/anaplastic meningiomas (5, 6) by immunohistochemistry. E, F: Quantitative analysis of RIZ1 and UbcH10 expression by immunohistochemistry in meningiomas of different grades. G: RIZ1 and UbcH10 expression showed a positive correlation in meningioma patient samples.

    Article Snippet: Then the sections were incubated with RIZ1 antibody (Abcam, UK) and UbcH10 antibody (Proteintech, USA) at 4°C overnight.

    Techniques: Expressing, Over Expression, Staining, Immunohistochemistry

    Silencing of UbcH10 inhibits cell proliferation, G2/M phase transition, migration and invasion in primary malignant meningioma cells. A, B: UbcH10 protein and mRNA level was down-regulated in siRNA group compared with NC group in M4 (WHO III) cells transfected with siRNA targeting UbcH10. WT: wild type cells; NC: scrambled siRNA transfected cells. C: siRNA targeting UbcH10 inhibited M4 (WHO III) cell growth as determined by WST-1 assay. D, E: Effect of siRNA-UbcH10 on cell cycle in M4 (WHO III) cells was detected through using flow cytometry, the population of G2/M phase was significantly increased after transfection. F-I: Silencing of UbcH10 inhibited cell migration and invasion in M4 (WHO III) cells. Data were based on at least 3 independent experiments, and shown as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001 as compared with NC).

    Journal: American Journal of Translational Research

    Article Title: RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma

    doi:

    Figure Lengend Snippet: Silencing of UbcH10 inhibits cell proliferation, G2/M phase transition, migration and invasion in primary malignant meningioma cells. A, B: UbcH10 protein and mRNA level was down-regulated in siRNA group compared with NC group in M4 (WHO III) cells transfected with siRNA targeting UbcH10. WT: wild type cells; NC: scrambled siRNA transfected cells. C: siRNA targeting UbcH10 inhibited M4 (WHO III) cell growth as determined by WST-1 assay. D, E: Effect of siRNA-UbcH10 on cell cycle in M4 (WHO III) cells was detected through using flow cytometry, the population of G2/M phase was significantly increased after transfection. F-I: Silencing of UbcH10 inhibited cell migration and invasion in M4 (WHO III) cells. Data were based on at least 3 independent experiments, and shown as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001 as compared with NC).

    Article Snippet: Then the sections were incubated with RIZ1 antibody (Abcam, UK) and UbcH10 antibody (Proteintech, USA) at 4°C overnight.

    Techniques: Sublimation, Migration, Transfection, WST-1 Assay, Flow Cytometry

    Effect of siRNA targeting UbcH10 on cell apoptosis in M4 (WHO III) cells. A: Induction of apoptosis was measured by PE/7-AAD staining at 24 h, 48 h, 72 h time point post transfection. B: Apoptotic cell population were significantly increased after UbcH10 siRNA transfection compared with NC group in M4 (WHO III) cells. C: Effect of UbcH10 siRNA on expression of Bax, Bcl-2, caspase3 protein in M4 (WHO III) cells, when transfected with UbcH10 siRNA 48 h, the expression of Bax and caspase3 were dramatically increased, whereas Bcl-2 expression was significantly decreased. Data were based on at least 3 independent experiments, and shown as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001 as compared with NC).

    Journal: American Journal of Translational Research

    Article Title: RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma

    doi:

    Figure Lengend Snippet: Effect of siRNA targeting UbcH10 on cell apoptosis in M4 (WHO III) cells. A: Induction of apoptosis was measured by PE/7-AAD staining at 24 h, 48 h, 72 h time point post transfection. B: Apoptotic cell population were significantly increased after UbcH10 siRNA transfection compared with NC group in M4 (WHO III) cells. C: Effect of UbcH10 siRNA on expression of Bax, Bcl-2, caspase3 protein in M4 (WHO III) cells, when transfected with UbcH10 siRNA 48 h, the expression of Bax and caspase3 were dramatically increased, whereas Bcl-2 expression was significantly decreased. Data were based on at least 3 independent experiments, and shown as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001 as compared with NC).

    Article Snippet: Then the sections were incubated with RIZ1 antibody (Abcam, UK) and UbcH10 antibody (Proteintech, USA) at 4°C overnight.

    Techniques: Staining, Transfection, Expressing

    RIZ1 regulates UbcH10 expression via c-Myc. A: c-Myc occupies the E-box of the UbcH10 prompter region, as measured by ChIP assay in M3 (WHO II) and M4 (WHO III) cells, primer 6 and primer 10 showed DNA positive strand. B: c-Myc binding sites in the UbcH10 promoter were located in -1500/-1201 bp and -300/-1 bp. C: Luciferase reporter gene assay indicated that co-transfection with pGL3-UbcH10 and pIRES2-c-Myc increased UbcH10 promoter activity, while the mutation constructs pGL3-UbcH10-mutation1 and pGL3-UbcH10-mutation2 both inhibited the transcriptional activity compared with pGL3-UbcH10. Co-transfected with pIRES2-c-Myc and pGL3-UbcH10-mutation1&2 did not make influence on the luciferase activity. The sketches of wild-type and mutated reporter constructs of UbcH10 were showed on the leftside. D, E: Transfection of pCMV-RIZ1 into M4 (WHO III) cells induced a significant decrease in UbcH10 expression, however, almost no reduction of UbcH10 expression was observed after transfection of pCMV-RIZ1 into the c-Myc shRNA-transduced M4 (WHO III) cells, both at the mRNA level and protein level. F: Luciferase reporter gene assay revealed that co-transfected with RIZ1 and pGL3-UbcH10 decreased the luciferase activity, whereas it exerted no significant effect on UbcH10 promoter activity when the reporter plasmid was pGL3-UbcH10-mutation1&2. G: Proposed model of the mechanism of RIZ1 inhibits the occurrence and development of meningioma via the c-Myc/UbcH10 axis. (*P<0.05, **P<0.01, ***P<0.001 as compared with NC).

    Journal: American Journal of Translational Research

    Article Title: RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma

    doi:

    Figure Lengend Snippet: RIZ1 regulates UbcH10 expression via c-Myc. A: c-Myc occupies the E-box of the UbcH10 prompter region, as measured by ChIP assay in M3 (WHO II) and M4 (WHO III) cells, primer 6 and primer 10 showed DNA positive strand. B: c-Myc binding sites in the UbcH10 promoter were located in -1500/-1201 bp and -300/-1 bp. C: Luciferase reporter gene assay indicated that co-transfection with pGL3-UbcH10 and pIRES2-c-Myc increased UbcH10 promoter activity, while the mutation constructs pGL3-UbcH10-mutation1 and pGL3-UbcH10-mutation2 both inhibited the transcriptional activity compared with pGL3-UbcH10. Co-transfected with pIRES2-c-Myc and pGL3-UbcH10-mutation1&2 did not make influence on the luciferase activity. The sketches of wild-type and mutated reporter constructs of UbcH10 were showed on the leftside. D, E: Transfection of pCMV-RIZ1 into M4 (WHO III) cells induced a significant decrease in UbcH10 expression, however, almost no reduction of UbcH10 expression was observed after transfection of pCMV-RIZ1 into the c-Myc shRNA-transduced M4 (WHO III) cells, both at the mRNA level and protein level. F: Luciferase reporter gene assay revealed that co-transfected with RIZ1 and pGL3-UbcH10 decreased the luciferase activity, whereas it exerted no significant effect on UbcH10 promoter activity when the reporter plasmid was pGL3-UbcH10-mutation1&2. G: Proposed model of the mechanism of RIZ1 inhibits the occurrence and development of meningioma via the c-Myc/UbcH10 axis. (*P<0.05, **P<0.01, ***P<0.001 as compared with NC).

    Article Snippet: Then the sections were incubated with RIZ1 antibody (Abcam, UK) and UbcH10 antibody (Proteintech, USA) at 4°C overnight.

    Techniques: Expressing, Binding Assay, Luciferase, Reporter Gene Assay, Cotransfection, Activity Assay, Mutagenesis, Construct, Transfection, shRNA, Plasmid Preparation