Journal: Biomolecules

Article Title: Long Noncoding RNA DANCR Activates Wnt/β-Catenin Signaling through MiR-216a Inhibition in Non-Small Cell Lung Cancer

doi: 10.3390/biom10121646

Figure Lengend Snippet: LncRNA-DANCR is a competitive inhibitor of miR-216a. ( A ) Potential binding position of DANCR MRE and miR-216a; ( B ) (top) schematic of MCP-MS2 immunoprecipitation assay; (bottom) MCP–MS2 immunoprecipitation assay using A549 DANCR KD cells transfected with MS2-DANCR compared to MS2-control cells ( n = 3, t test, *** p < 0.001); ( C ) (top) schematic of luciferase-DANCR reporter assay; (bottom) 293T cells cotransfected with luciferase-DANCR reporter + miR-216a expression plasmid compared to luciferase-DANCR reporter + vector control cells ( n = 3, t test, *** p < 0.001); ( D ) fold change of miR-216a expression in NSCLC cell lines compared to lung epithelial cell line HBE2 ( n = 3, t test, *** p < 0.001, **** p < 0.0001).

Article Snippet: The day before, 293T cells were plated in a 12 well plate and then cotransfected with pSGG-luc-DANCR construct and the Renilla vector, along with the p-Babe-miR-216a plasmid (Addgene 65054) using Lipofectamine 2000.

Techniques: Binding Assay, Immunoprecipitation, Transfection, Luciferase, Reporter Assay, Expressing, Plasmid Preparation

Journal: Biomolecules

Article Title: Long Noncoding RNA DANCR Activates Wnt/β-Catenin Signaling through MiR-216a Inhibition in Non-Small Cell Lung Cancer

doi: 10.3390/biom10121646

Figure Lengend Snippet: DANCR promotes Wnt/β-catenin signaling through miR-216a inhibition. ( A ) qRT-PCR measuring fold change of DANCR expression in A549 cells transfected with pBABE-DANCR compared to vector control ( n = 3; t test, ** p < 0.001); ( B ) fold change of β-catenin protein expression in A549 DANCR overexpressing cells ( n = 3, t test, ** p < 0.001); ( C ) (left) qRT-PCR measuring fold change of DANCR expression in A549 cells doubly transfected with vector + vector (EV + EV), vector + pBABE-miR-216a (EV + miR-216a), vector + pBABE-DANCR (EV + DANCR), or pBABE-DANCR + pBABE-miR-216a (DANCR + miR-216a); (right) qRT-PCR measuring fold change of miR-216a expression ( n = 4, t test, * p < 0.05, ** p < 0.001); ( D ) fold change of β-catenin protein expression in A549 cells doubly transfected with vector + vector (EV + EV), vector + pBABE-miR-216a (EV + miR-216a), vector + pBABE-DANCR (EV + DANCR), or pBABE-DANCR + pBABE-miR-216a (DANCR + miR-216a) ( n = 4, t test, * p < 0.05).

Article Snippet: The day before, 293T cells were plated in a 12 well plate and then cotransfected with pSGG-luc-DANCR construct and the Renilla vector, along with the p-Babe-miR-216a plasmid (Addgene 65054) using Lipofectamine 2000.

Techniques: Inhibition, Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation

Journal: bioRxiv

Article Title: Reemergence of the Murine Bacterial Pathogen Chlamydia muridarum in Laboratory Mouse Colonies

doi: 10.1101/2022.05.16.491822

Figure Lengend Snippet: Fluorescent images of Chlamydia -infected cells. HeLa 229 cells were infected with Chlamydia spp. isolated with an NSG mouse cecum sample. At 30 hours post-infection, cells were fixed and stained with FITC-labeled anti -Chlamydia spp. antibody (green) and analyzed using an EVOS™ FL Auto Imaging fluorescent microscope (Life Technologies).

Article Snippet: Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.

Techniques: Infection, Isolation, Staining, Labeling, Imaging, Microscopy