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    Addgene inc streptococcus pyogenes dcas9 sequence
    Streptococcus Pyogenes Dcas9 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology pinch 1 sirna
    Expression of nephrin, phosphorylated nephrin, PIP complex and F-actin in rats with PAN. (A) Immunofluorescence detection of glomerular nephrin expression in different groups. Original magnification ×400. (B) Western blot for phosphorylated nephrin and nephrin in the glomeruli of rats of different groups. *P < 0.05 compared with the group of day 0. (C) Co-immunoprecipitation of the PIP complex in the glomeruli of rats of different groups. ILK and <t>PINCH-1</t> were immunoprecipitated by an α-parvin antibody. *P < 0.05 compared with the group of day 0. (D) Western blot for F-actin expression in the glomeruli of rats of different groups. *P < 0.05 compared with the group of day 0.
    Pinch 1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression of nephrin, phosphorylated nephrin, PIP complex and F-actin in rats with PAN. (A) Immunofluorescence detection of glomerular nephrin expression in different groups. Original magnification ×400. (B) Western blot for phosphorylated nephrin and nephrin in the glomeruli of rats of different groups. *P < 0.05 compared with the group of day 0. (C) Co-immunoprecipitation of the PIP complex in the glomeruli of rats of different groups. ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody. *P < 0.05 compared with the group of day 0. (D) Western blot for F-actin expression in the glomeruli of rats of different groups. *P < 0.05 compared with the group of day 0.

    Journal: BMB Reports

    Article Title: Nephrin phosphorylation regulates podocyte adhesion through the PINCH-1-ILK-α-parvin complex

    doi: 10.5483/BMBRep.2013.46.4.270

    Figure Lengend Snippet: Expression of nephrin, phosphorylated nephrin, PIP complex and F-actin in rats with PAN. (A) Immunofluorescence detection of glomerular nephrin expression in different groups. Original magnification ×400. (B) Western blot for phosphorylated nephrin and nephrin in the glomeruli of rats of different groups. *P < 0.05 compared with the group of day 0. (C) Co-immunoprecipitation of the PIP complex in the glomeruli of rats of different groups. ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody. *P < 0.05 compared with the group of day 0. (D) Western blot for F-actin expression in the glomeruli of rats of different groups. *P < 0.05 compared with the group of day 0.

    Article Snippet: PINCH-1 siRNA and nephrin siRNA transfection was performed according to the Santa Cruz transfection reagent handbook (Santa Cruz, USA).

    Techniques: Expressing, Immunofluorescence, Western Blot, Immunoprecipitation

    Knockdown of the PIP complex resulted in the reorganization of the cytoskeleton, and down-regulated podocyte adhesion and spreading. (A) ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody in cultured normal podocytes and podocytes stimulated by PA with/without PINCH-1 siRNA or scrambled siRNA transfection. The immunoprecipitates were analyzed by Western blot with antibodies of ILK, PINCH-1 and α-parvin. *P < 0.05 compared with the normal group; # P < 0.05 compared with the groups stimulated by PA with/without scrambled siRNA. (B) Nephrin and phosphorylated nephrin were detected by Western blot in different groups. *P < 0.05 compared with the normal group; # P < 0.05 compared with the groups stimulated by PA with/without scrambled siRNA. (C) Stress fiber of the cytoskeleton was detected by immunofluorescence with FITC-phalloidin. Original magnification ×400. (a) Normal podocytes; (b) podocytes transfected with PINCH-1 siRNA; (c) podocytes transfected with scrambled siRNA; (d) podocytes stimulated by PA; (e) podocytes stimulated by PA with PINCH-1 siRNA transfection; (f) podocytes stimulated by PA with scrambled siRNA transfection. (D) Cell adhesion was measured with spectrophotometry. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group. (E) Cell spreading was detected and counted under inverted microscope. Original magnification ×400. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group.

    Journal: BMB Reports

    Article Title: Nephrin phosphorylation regulates podocyte adhesion through the PINCH-1-ILK-α-parvin complex

    doi: 10.5483/BMBRep.2013.46.4.270

    Figure Lengend Snippet: Knockdown of the PIP complex resulted in the reorganization of the cytoskeleton, and down-regulated podocyte adhesion and spreading. (A) ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody in cultured normal podocytes and podocytes stimulated by PA with/without PINCH-1 siRNA or scrambled siRNA transfection. The immunoprecipitates were analyzed by Western blot with antibodies of ILK, PINCH-1 and α-parvin. *P < 0.05 compared with the normal group; # P < 0.05 compared with the groups stimulated by PA with/without scrambled siRNA. (B) Nephrin and phosphorylated nephrin were detected by Western blot in different groups. *P < 0.05 compared with the normal group; # P < 0.05 compared with the groups stimulated by PA with/without scrambled siRNA. (C) Stress fiber of the cytoskeleton was detected by immunofluorescence with FITC-phalloidin. Original magnification ×400. (a) Normal podocytes; (b) podocytes transfected with PINCH-1 siRNA; (c) podocytes transfected with scrambled siRNA; (d) podocytes stimulated by PA; (e) podocytes stimulated by PA with PINCH-1 siRNA transfection; (f) podocytes stimulated by PA with scrambled siRNA transfection. (D) Cell adhesion was measured with spectrophotometry. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group. (E) Cell spreading was detected and counted under inverted microscope. Original magnification ×400. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group.

    Article Snippet: PINCH-1 siRNA and nephrin siRNA transfection was performed according to the Santa Cruz transfection reagent handbook (Santa Cruz, USA).

    Techniques: Immunoprecipitation, Cell Culture, Transfection, Western Blot, Immunofluorescence, Spectrophotometry, Inverted Microscopy

    Diminishment of phosphorylated nephrin resulted in cytoskeleton reorganization, decreased podocyte adhesion and spreading in podocyte injury. (A) Nephrin and phosphorylated nephrin were detected by Western blot in different groups. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group. (B) ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody in different groups. The immunoprecipitates were analyzed by Western blot with antibodies against ILK and PINCH-1. *P < 0.05 compared with the control group; # P < 0.05 compared with the PA or scrambled siRNA group. (C) Stress fiber of the cytoskeleton was detected by immunofluorescence with FITC-phalloidin. Original magnification ×400. (a) Normal podocytes; (b) podocytes transfected with nephrin siRNA; (c) podocytes transfected with scrambled siRNA; (d) podocytes stimulated by PA; (e) podocytes stimulated by PA with nephrin siRNA transfection; (f) podocytes stimulated by PA with scrambled siRNA transfection. (D) Cell adhesion was measured with a spectrophotometer. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group. (E) Cell spreading was detected and counted under an inverted microscope. Original magnification ×400. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group.

    Journal: BMB Reports

    Article Title: Nephrin phosphorylation regulates podocyte adhesion through the PINCH-1-ILK-α-parvin complex

    doi: 10.5483/BMBRep.2013.46.4.270

    Figure Lengend Snippet: Diminishment of phosphorylated nephrin resulted in cytoskeleton reorganization, decreased podocyte adhesion and spreading in podocyte injury. (A) Nephrin and phosphorylated nephrin were detected by Western blot in different groups. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group. (B) ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody in different groups. The immunoprecipitates were analyzed by Western blot with antibodies against ILK and PINCH-1. *P < 0.05 compared with the control group; # P < 0.05 compared with the PA or scrambled siRNA group. (C) Stress fiber of the cytoskeleton was detected by immunofluorescence with FITC-phalloidin. Original magnification ×400. (a) Normal podocytes; (b) podocytes transfected with nephrin siRNA; (c) podocytes transfected with scrambled siRNA; (d) podocytes stimulated by PA; (e) podocytes stimulated by PA with nephrin siRNA transfection; (f) podocytes stimulated by PA with scrambled siRNA transfection. (D) Cell adhesion was measured with a spectrophotometer. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group. (E) Cell spreading was detected and counted under an inverted microscope. Original magnification ×400. *P < 0.05 compared with the normal group; # P < 0.05 compared with the PA or scrambled siRNA group.

    Article Snippet: PINCH-1 siRNA and nephrin siRNA transfection was performed according to the Santa Cruz transfection reagent handbook (Santa Cruz, USA).

    Techniques: Western Blot, Immunoprecipitation, Immunofluorescence, Transfection, Spectrophotometry, Inverted Microscopy

    Effect of phosphorylated nephrin on podocyte cytoskeleton, adhesion and spreading in the physiological state. (A) Phosphorylated nephrin was detected by Western blot with antibody against phosphorylated nephrin (pY1217) in cultured normal podocytes and podocytes stimulated by the Src family kinase inhibitor (PP2). *P < 0.05 compared with the normal group. (B) ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody in cultured normal podocytes and podocytes stimulated by PP2. The immunoprecipitates were analyzed by Western blot with antibodies against ILK, PINCH-1 and α-parvin. *P < 0.05 compared with the normal group. (C) Stress fiber of the cytoskeleton was detected by immunofluorescence with FITC-phalloidin. Original magnification ×400. (D) Cell adhesion was measured with a spectrophotometer. *P < 0.05 compared with the normal group. (E) Cell spreading was detected and counted under an inverted microscope. Original magnification ×400. *P < 0.05 compared with the normal group.

    Journal: BMB Reports

    Article Title: Nephrin phosphorylation regulates podocyte adhesion through the PINCH-1-ILK-α-parvin complex

    doi: 10.5483/BMBRep.2013.46.4.270

    Figure Lengend Snippet: Effect of phosphorylated nephrin on podocyte cytoskeleton, adhesion and spreading in the physiological state. (A) Phosphorylated nephrin was detected by Western blot with antibody against phosphorylated nephrin (pY1217) in cultured normal podocytes and podocytes stimulated by the Src family kinase inhibitor (PP2). *P < 0.05 compared with the normal group. (B) ILK and PINCH-1 were immunoprecipitated by an α-parvin antibody in cultured normal podocytes and podocytes stimulated by PP2. The immunoprecipitates were analyzed by Western blot with antibodies against ILK, PINCH-1 and α-parvin. *P < 0.05 compared with the normal group. (C) Stress fiber of the cytoskeleton was detected by immunofluorescence with FITC-phalloidin. Original magnification ×400. (D) Cell adhesion was measured with a spectrophotometer. *P < 0.05 compared with the normal group. (E) Cell spreading was detected and counted under an inverted microscope. Original magnification ×400. *P < 0.05 compared with the normal group.

    Article Snippet: PINCH-1 siRNA and nephrin siRNA transfection was performed according to the Santa Cruz transfection reagent handbook (Santa Cruz, USA).

    Techniques: Western Blot, Cell Culture, Immunoprecipitation, Immunofluorescence, Spectrophotometry, Inverted Microscopy