Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Imaging data of Di-8-ANEPPQ labeled primary neuronal and cardiac cells in vitro The Di-8-ANEPPQ dye labeling efficiency was determined by flourescent imaging of the primary NG neurons (A) or primary neonatal cardiomyocytes (B) imaged by floresence microscopy. Di-8-ANEPPQ: green, scale bar: 100 µm.

Article Snippet: Di-8-ANEPPQ (61014, Biotium) at a stock solution of 10 mM was prepared to add 697 µl DMSO into the 5 mg dye powder, then vortexed and stored at −20 °C in small aliquots.

Techniques: Imaging, Labeling, In Vitro, Microscopy

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Emission spectrum analysis of Di-8-ANEPPQ labeled primary cell culture. Di-8-ANEPPQ (gray) labeled cardiac cells were excited (A) 488 nm, (B) 561 nm, and (C) 633 nm. Emission spectrum data between 415 nm and 690 nm were captured and displayed. Scale bars: 100 µm.

Article Snippet: Di-8-ANEPPQ (61014, Biotium) at a stock solution of 10 mM was prepared to add 697 µl DMSO into the 5 mg dye powder, then vortexed and stored at −20 °C in small aliquots.

Techniques: Labeling, Cell Culture

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Experimental steps for application of a retrograde dye to the heart in vivo. (A) The anesthetized mouse was shaved at the operation area and the skin was aseptically cleaned. (B) The mouse was intubated using a cannula and connected to the ventilation apparatus. (C) The incisions were made at the left side of the thorax above the xiphoid, and muscles were moved gently to expose the heart. (D) The retractors were placed to separate the third and fourth ribs. (E) T he Di-8-ANEPPQ was injected into apex or (F) DiI paste was applied on the heart. Arrow showed the tissue after DiI paste was applied. (G) The application area observed by the spread of the retrograde dye in the cardiac tissue. The injection site is shown by the arrow. (H) The intercostal muscles and (I) the skin were sutured.

Article Snippet: Di-8-ANEPPQ (61014, Biotium) at a stock solution of 10 mM was prepared to add 697 µl DMSO into the 5 mg dye powder, then vortexed and stored at −20 °C in small aliquots.

Techniques: In Vivo, Muscles, Injection

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Imaging data of Di-8-ANEPPQ from in vivo labeled cardiac tissue . Fluorescence imaging was performed on unlabeled control heart tissue for determining (A) the background or (B) following the Di-8-ANEPPQ administration to the murine heart in vivo by confocal microcopy. Di-8-ANEPPQ: green. Scale bar: 100 µm.

Article Snippet: Di-8-ANEPPQ (61014, Biotium) at a stock solution of 10 mM was prepared to add 697 µl DMSO into the 5 mg dye powder, then vortexed and stored at −20 °C in small aliquots.

Techniques: Imaging, In Vivo, Labeling, Fluorescence

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet:

Article Snippet: Di-8-ANEPPQ (61014, Biotium) at a stock solution of 10 mM was prepared to add 697 µl DMSO into the 5 mg dye powder, then vortexed and stored at −20 °C in small aliquots.

Techniques: In Vitro, In Vivo, Microscopy, Software, Staining, Labeling, Muscles, Injection, Incubation, Cell Culture, Confocal Microscopy

Journal:

Article Title: Production of Wax Esters during Aerobic Growth of Marine Bacteria on Isoprenoid Compounds

doi:

Figure Lengend Snippet: Proposed pathways for the production of isoprenoid wax esters during the metabolism of 6,10,14-trimethylpentadecan-2-one. (The processes for formation of esters 16 and 17 were omitted in order to simplify the scheme.)

Article Snippet: Like Marinobacter sp. strain CAB (DSMZ 11874) ( 43 ) and Acinetobacter sp. strain PHY9 ( 41 ), P. nautica (IP85/617) and M. hydrocarbonoclasticus (ATCC 49840) were able to grow on 6,10,14-trimethylpentadecan-2-one (compound 1) as the sole carbon and energy source under aerobic conditions.

Techniques:

Journal:

Article Title: Production of Wax Esters during Aerobic Growth of Marine Bacteria on Isoprenoid Compounds

doi:

Figure Lengend Snippet: Electron impact mass spectra of (E)-4,8,12-trimethyltridecyl-4,8,12-trimethyltridec-3-enoate (compound 14) (A), 4,8,12-trimethyltridecyl-4,8,12-trimethyltridecanoate (compound 11) (B), and 6,10,14-trimethylpentadec-2-yl-4,8,12-trimethyl-tridecanoate (compound 15) (C).

Article Snippet: Like Marinobacter sp. strain CAB (DSMZ 11874) ( 43 ) and Acinetobacter sp. strain PHY9 ( 41 ), P. nautica (IP85/617) and M. hydrocarbonoclasticus (ATCC 49840) were able to grow on 6,10,14-trimethylpentadecan-2-one (compound 1) as the sole carbon and energy source under aerobic conditions.

Techniques:

Journal:

Article Title: Production of Wax Esters during Aerobic Growth of Marine Bacteria on Isoprenoid Compounds

doi:

Figure Lengend Snippet: Partial total ion chromatograms showing the wax ester region of the extracts obtained after growth of P. nautica on 6,10,14-trimethylpentadecan-2-one (compound 1) with ammonium ion concentrations of 56 mM (A) and 0.1 mM (B). IS, internal standard (triacontane).

Article Snippet: Like Marinobacter sp. strain CAB (DSMZ 11874) ( 43 ) and Acinetobacter sp. strain PHY9 ( 41 ), P. nautica (IP85/617) and M. hydrocarbonoclasticus (ATCC 49840) were able to grow on 6,10,14-trimethylpentadecan-2-one (compound 1) as the sole carbon and energy source under aerobic conditions.

Techniques:

Journal: Cell reports

Article Title: Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy

doi: 10.1016/j.celrep.2022.110797

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-DNA mouse monoclonal, AC-30-10, lyophilized, purified , Progen , 61014; RRID: AB_2750935.

Techniques: Purification, Recombinant, Protease Inhibitor, Magnetic Beads, Lysis, Western Blot, Stripping, Staining, Transfection, Luciferase, Isolation, In Situ, Mutagenesis, Knock-Out, Stable Transfection, Expressing, Software, Imaging