Journal: The Journal of allergy and clinical immunology
Article Title: B-cell development and functions and therapeutic options in adenosine deaminase–deficient patients
Figure Lengend Snippet: Impaired B-cell proliferation after BCR/TLR triggering in patients undergoing ERT is corrected after HSC-GT. A, Percentage of CFSE-diluting B cells in patients undergoing HSC-GT (n = 4 for CpG stimulation and n = 6 when either immunoglobulin or CD40L
Article Snippet: CFSE-stained B cells (6 × 104 ) from patients undergoing HSC-GT and donors were plated in 96-well flat-bottom culture plates and stimulated for 72 hours in RPMI 10% Hyclone (Sera; Thermo Scientific, Uppsala, Sweden) with 2.5 μg/mL CpG2006 (5′-TsCsg sTsGsg sTsTsT sTsgsT sCsgsT sTsTsT sgsTsC sgsTsT-3′; TIB BioMol, Genoa, Italy), 2.5 μg/mL F(ab′)2 anti-human IgM/IgG/IgA (Jackson Immunoresearch), and 3 ng/mL CD40L (Alexis Biochemicals, San Diego, Calif).