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    • propionic acid  (Millipore)
    94
    Millipore propionic acid
    Propionic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/propionic acid/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    propionic acid - by Bioz Stars, 2022-08
    94/100 stars
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    cd40l  (Enzo Biochem)
    97
    Enzo Biochem cd40l
    Spermidine Induces TFEB Expression and Improves the Function of Old Human B Cells (A) The protein levels of TFEB, hypusinated eIF5A, and overall eIF5A of PBMCs from healthy human donors of indicated ages were assessed using western blot. A representative plot (left) and quantifications (right) are shown. n = 8–15 donors. (B) TFEB mRNA of human PBMCs was measured using qPCR with GAPDH as the reference gene. n = 7–9 donors. (C) Sorted B cells from young human donors were cultured with <t>anti-IgM/CD40L</t> and treated with DFMO with or without 10 μM spermidine for 7 days. n = 5 donors. (D) Sorted B cells from human donors were cultured as in (C) with spermidine and/or GC7 as indicated. The expression of hypusinated (Hyp) or non-hypusinated (AcLys or Lys) eIF5A was distinguished by two-dimensional western blot of eIF5A. Black arrow, hypusinated Lys 50 , pH 5.2; red arrow, unmodified Lys 50 , pH 5.1; blue arrow, acetylated Lys 47 with unmodified Lys 50 , pH 5.0. The non-hypusination ratio was calculated as eIF5A dot densitometric intensity (AcLys + Lys)/(AcLys + Lys + Hyp). n = 4 donors. (E–G) Sorted B cells from old human donors (age 77.5 ± 6.3 years) were cultured as in (C) together with spermidine and GC7. (E) The protein levels of TFEB and eIF5A hypusination were measured using western blot. (F) Autophagic flux was determined by flow cytometry as in Figure 1 A. (G) Supernatant IgG was assessed using ELISA. n = 4–14 donors. Data are represented as mean ± SEM. Unpaired two-tailed Student’s t test (A and D, comparison of young versus old). Welch’s t test (B). Paired one-way ANOVA with post hoc Tukey’s test (C). Paired one-tailed t test (D, comparisons of old versus old + Spd, old + Spd versus old + Spd + GC7 comparison, E–G). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. See also Figure S7 .
    Cd40l, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd40l/product/Enzo Biochem
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd40l - by Bioz Stars, 2022-08
    97/100 stars
    		  Buy from Supplier
    cd40l  (Enzo Life Sciences)
    93
    Enzo Life Sciences cd40l
    Spermidine Induces TFEB Expression and Improves the Function of Old Human B Cells (A) The protein levels of TFEB, hypusinated eIF5A, and overall eIF5A of PBMCs from healthy human donors of indicated ages were assessed using western blot. A representative plot (left) and quantifications (right) are shown. n = 8–15 donors. (B) TFEB mRNA of human PBMCs was measured using qPCR with GAPDH as the reference gene. n = 7–9 donors. (C) Sorted B cells from young human donors were cultured with <t>anti-IgM/CD40L</t> and treated with DFMO with or without 10 μM spermidine for 7 days. n = 5 donors. (D) Sorted B cells from human donors were cultured as in (C) with spermidine and/or GC7 as indicated. The expression of hypusinated (Hyp) or non-hypusinated (AcLys or Lys) eIF5A was distinguished by two-dimensional western blot of eIF5A. Black arrow, hypusinated Lys 50 , pH 5.2; red arrow, unmodified Lys 50 , pH 5.1; blue arrow, acetylated Lys 47 with unmodified Lys 50 , pH 5.0. The non-hypusination ratio was calculated as eIF5A dot densitometric intensity (AcLys + Lys)/(AcLys + Lys + Hyp). n = 4 donors. (E–G) Sorted B cells from old human donors (age 77.5 ± 6.3 years) were cultured as in (C) together with spermidine and GC7. (E) The protein levels of TFEB and eIF5A hypusination were measured using western blot. (F) Autophagic flux was determined by flow cytometry as in Figure 1 A. (G) Supernatant IgG was assessed using ELISA. n = 4–14 donors. Data are represented as mean ± SEM. Unpaired two-tailed Student’s t test (A and D, comparison of young versus old). Welch’s t test (B). Paired one-way ANOVA with post hoc Tukey’s test (C). Paired one-tailed t test (D, comparisons of old versus old + Spd, old + Spd versus old + Spd + GC7 comparison, E–G). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. See also Figure S7 .
    Cd40l, supplied by Enzo Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd40l/product/Enzo Life Sciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd40l - by Bioz Stars, 2022-08
    93/100 stars
    		  Buy from Supplier
    potassium antimonyl tartrate  (Millipore)
    93
    Millipore potassium antimonyl tartrate
    Spermidine Induces TFEB Expression and Improves the Function of Old Human B Cells (A) The protein levels of TFEB, hypusinated eIF5A, and overall eIF5A of PBMCs from healthy human donors of indicated ages were assessed using western blot. A representative plot (left) and quantifications (right) are shown. n = 8–15 donors. (B) TFEB mRNA of human PBMCs was measured using qPCR with GAPDH as the reference gene. n = 7–9 donors. (C) Sorted B cells from young human donors were cultured with <t>anti-IgM/CD40L</t> and treated with DFMO with or without 10 μM spermidine for 7 days. n = 5 donors. (D) Sorted B cells from human donors were cultured as in (C) with spermidine and/or GC7 as indicated. The expression of hypusinated (Hyp) or non-hypusinated (AcLys or Lys) eIF5A was distinguished by two-dimensional western blot of eIF5A. Black arrow, hypusinated Lys 50 , pH 5.2; red arrow, unmodified Lys 50 , pH 5.1; blue arrow, acetylated Lys 47 with unmodified Lys 50 , pH 5.0. The non-hypusination ratio was calculated as eIF5A dot densitometric intensity (AcLys + Lys)/(AcLys + Lys + Hyp). n = 4 donors. (E–G) Sorted B cells from old human donors (age 77.5 ± 6.3 years) were cultured as in (C) together with spermidine and GC7. (E) The protein levels of TFEB and eIF5A hypusination were measured using western blot. (F) Autophagic flux was determined by flow cytometry as in Figure 1 A. (G) Supernatant IgG was assessed using ELISA. n = 4–14 donors. Data are represented as mean ± SEM. Unpaired two-tailed Student’s t test (A and D, comparison of young versus old). Welch’s t test (B). Paired one-way ANOVA with post hoc Tukey’s test (C). Paired one-tailed t test (D, comparisons of old versus old + Spd, old + Spd versus old + Spd + GC7 comparison, E–G). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. See also Figure S7 .
    Potassium Antimonyl Tartrate, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/potassium antimonyl tartrate/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    potassium antimonyl tartrate - by Bioz Stars, 2022-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Spermidine Induces TFEB Expression and Improves the Function of Old Human B Cells (A) The protein levels of TFEB, hypusinated eIF5A, and overall eIF5A of PBMCs from healthy human donors of indicated ages were assessed using western blot. A representative plot (left) and quantifications (right) are shown. n = 8–15 donors. (B) TFEB mRNA of human PBMCs was measured using qPCR with GAPDH as the reference gene. n = 7–9 donors. (C) Sorted B cells from young human donors were cultured with anti-IgM/CD40L and treated with DFMO with or without 10 μM spermidine for 7 days. n = 5 donors. (D) Sorted B cells from human donors were cultured as in (C) with spermidine and/or GC7 as indicated. The expression of hypusinated (Hyp) or non-hypusinated (AcLys or Lys) eIF5A was distinguished by two-dimensional western blot of eIF5A. Black arrow, hypusinated Lys 50 , pH 5.2; red arrow, unmodified Lys 50 , pH 5.1; blue arrow, acetylated Lys 47 with unmodified Lys 50 , pH 5.0. The non-hypusination ratio was calculated as eIF5A dot densitometric intensity (AcLys + Lys)/(AcLys + Lys + Hyp). n = 4 donors. (E–G) Sorted B cells from old human donors (age 77.5 ± 6.3 years) were cultured as in (C) together with spermidine and GC7. (E) The protein levels of TFEB and eIF5A hypusination were measured using western blot. (F) Autophagic flux was determined by flow cytometry as in Figure 1 A. (G) Supernatant IgG was assessed using ELISA. n = 4–14 donors. Data are represented as mean ± SEM. Unpaired two-tailed Student’s t test (A and D, comparison of young versus old). Welch’s t test (B). Paired one-way ANOVA with post hoc Tukey’s test (C). Paired one-tailed t test (D, comparisons of old versus old + Spd, old + Spd versus old + Spd + GC7 comparison, E–G). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. See also Figure S7 .

    Journal: Molecular Cell

    Article Title: Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence

    doi: 10.1016/j.molcel.2019.08.005

    Figure Lengend Snippet: Spermidine Induces TFEB Expression and Improves the Function of Old Human B Cells (A) The protein levels of TFEB, hypusinated eIF5A, and overall eIF5A of PBMCs from healthy human donors of indicated ages were assessed using western blot. A representative plot (left) and quantifications (right) are shown. n = 8–15 donors. (B) TFEB mRNA of human PBMCs was measured using qPCR with GAPDH as the reference gene. n = 7–9 donors. (C) Sorted B cells from young human donors were cultured with anti-IgM/CD40L and treated with DFMO with or without 10 μM spermidine for 7 days. n = 5 donors. (D) Sorted B cells from human donors were cultured as in (C) with spermidine and/or GC7 as indicated. The expression of hypusinated (Hyp) or non-hypusinated (AcLys or Lys) eIF5A was distinguished by two-dimensional western blot of eIF5A. Black arrow, hypusinated Lys 50 , pH 5.2; red arrow, unmodified Lys 50 , pH 5.1; blue arrow, acetylated Lys 47 with unmodified Lys 50 , pH 5.0. The non-hypusination ratio was calculated as eIF5A dot densitometric intensity (AcLys + Lys)/(AcLys + Lys + Hyp). n = 4 donors. (E–G) Sorted B cells from old human donors (age 77.5 ± 6.3 years) were cultured as in (C) together with spermidine and GC7. (E) The protein levels of TFEB and eIF5A hypusination were measured using western blot. (F) Autophagic flux was determined by flow cytometry as in Figure 1 A. (G) Supernatant IgG was assessed using ELISA. n = 4–14 donors. Data are represented as mean ± SEM. Unpaired two-tailed Student’s t test (A and D, comparison of young versus old). Welch’s t test (B). Paired one-way ANOVA with post hoc Tukey’s test (C). Paired one-tailed t test (D, comparisons of old versus old + Spd, old + Spd versus old + Spd + GC7 comparison, E–G). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. See also Figure S7 .

    Article Snippet: B cells (5 × 105 cells) were seeded in 24-well plates, then activated with anti-IgM (5 μg/ml, Jackson Immuno Research) and CD40L (100 ng/ml, Enzo Life science) and treated with 1 mM DFMO, 10 μM spermidine, or 10 μM GC7 for 7 days.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Cell Culture, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Two Tailed Test, One-tailed Test