60-mm-diameter Search Results


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  • 91
    Greiner Bio 60 mm diameter dishes
    60 Mm Diameter Dishes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 91/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60 mm diameter dishes/product/Greiner Bio
    Average 91 stars, based on 63 article reviews
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    60 mm diameter dishes - by Bioz Stars, 2020-07
    91/100 stars
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    90
    Corning Life Sciences 60 mm diameter dishes
    60 Mm Diameter Dishes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60 mm diameter dishes/product/Corning Life Sciences
    Average 90 stars, based on 15 article reviews
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    60 mm diameter dishes - by Bioz Stars, 2020-07
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    90
    Magtron Inc 60 mm diameter gradient coil
    60 Mm Diameter Gradient Coil, supplied by Magtron Inc, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 23 article reviews
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    60 mm diameter gradient coil - by Bioz Stars, 2020-07
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    92
    Avantor 60 mm diameter petri dishes
    60 Mm Diameter Petri Dishes, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    60 mm diameter petri dishes - by Bioz Stars, 2020-07
    92/100 stars
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    90
    Corning Life Sciences 60 mm diameter
    60 Mm Diameter, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60 mm diameter/product/Corning Life Sciences
    Average 90 stars, based on 10 article reviews
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    60 mm diameter - by Bioz Stars, 2020-07
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    92
    Iwaki America 60 mm diameter culture dishes
    60 Mm Diameter Culture Dishes, supplied by Iwaki America, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 4 article reviews
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    60 mm diameter culture dishes - by Bioz Stars, 2020-07
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    88
    Becton Dickinson 60 mm diameter polystyrene dishes
    60 Mm Diameter Polystyrene Dishes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 20 article reviews
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    60 mm diameter polystyrene dishes - by Bioz Stars, 2020-07
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    90
    Roche 60 mm diameter dishes
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Dishes, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 71 article reviews
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    60 mm diameter dishes - by Bioz Stars, 2020-07
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    90
    Becton Dickinson 60 mm diameter dishes
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Dishes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 16 article reviews
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    60 mm diameter dishes - by Bioz Stars, 2020-07
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    88
    Becton Dickinson 60 mm diameter plate
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Plate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 3 article reviews
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    60 mm diameter plate - by Bioz Stars, 2020-07
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    85
    Biocoat 60 mm diameter collagen coated dishes
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Collagen Coated Dishes, supplied by Biocoat, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 2 article reviews
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    60 mm diameter collagen coated dishes - by Bioz Stars, 2020-07
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    84
    Covidien 60 mm diameter linear stapler
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Linear Stapler, supplied by Covidien, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 1 article reviews
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    60 mm diameter linear stapler - by Bioz Stars, 2020-07
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    88
    Becton Dickinson 60 mm diameter petri dishes
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Petri Dishes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 10 article reviews
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    60 mm diameter petri dishes - by Bioz Stars, 2020-07
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    85
    Corning Life Sciences 60 mm diameter petri dish
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Petri Dish, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60 mm diameter petri dish/product/Corning Life Sciences
    Average 85 stars, based on 7 article reviews
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    60 mm diameter petri dish - by Bioz Stars, 2020-07
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    80
    Difco 60 mm diameter dishes nunc
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Dishes Nunc, supplied by Difco, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 2 article reviews
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    60 mm diameter dishes nunc - by Bioz Stars, 2020-07
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    85
    Hirschmann 60 mm diameter glass coverslips
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Glass Coverslips, supplied by Hirschmann, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    60 mm diameter glass coverslips - by Bioz Stars, 2020-07
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    80
    Becton Dickinson 60 mm diameter tissue culture dish
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Tissue Culture Dish, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    60 mm diameter tissue culture dish - by Bioz Stars, 2020-07
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    88
    Corning Life Sciences 60 mm diameter petri dishes
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Petri Dishes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    60 mm diameter petri dishes - by Bioz Stars, 2020-07
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    91
    Fisher Scientific 60 mm diameter petri dish
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Petri Dish, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biocoat 60 mm diameter collagen type i coated dish
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Collagen Type I Coated Dish, supplied by Biocoat, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biocoat 60 mm diameter poly l lysine coated plates
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
    60 Mm Diameter Poly L Lysine Coated Plates, supplied by Biocoat, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 60 mm diameter culture dish
    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in <t>60-mm-diameter</t> dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.
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    Image Search Results


    Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in 60-mm-diameter dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.

    Journal: The Biochemical Journal

    Article Title: Regulation of Cidea protein stability by the ubiquitin-mediated proteasomal degradation pathway

    doi: 10.1042/BJ20070690

    Figure Lengend Snippet: Cidea K23 is the major contributor to protein instability ( A ) Amino acid sequence alignment of Cidea sequence in mammals using ClustalX (version 1.83) software. The shaded regions indicate identical amino acids and conserved lysine residues. The proposed Cidea Cide-N domains are overlined. ( B ) A 1.0 μg portion of CMV5–HA vector, CMV5–HA–Cidea and CMV–HA–Cidea–K23A, CMV–HA–Cidea–K23E, CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R was co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in 60-mm-diameter dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham) and results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( D ) HEK-293T cells were co-transfected with 1.0 μg of CMV5–HA control vector, CMV5–HA–Cidea, CMV5–HA–Cidea–K23A, CMV–HA–Cidea–K23E, or CMV–HA–Cidea–K23G or CMV–HA–Cidea–K23R with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then an in vivo ubiquitination assay was performed as described above and cell lysates were subjected to IP (immunoprecipitation) using anti-HA and Protein A/G Plus–agarose beads. The immunoprecipites were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. IB, immunoblotting.

    Article Snippet: Transfections were performed in 60-mm-diameter dishes using either Dosper reagent (Roche) or the calcium phosphate method [ ].

    Techniques: Sequencing, Software, Plasmid Preparation, Transfection, Western Blot, In Vivo, Ubiquitin Assay, Immunoprecipitation

    Cidea N-terminal lysine residues play a predominant role in controlling protein stability ( A ) Schematic drawing of human Cidea, WT, N-5KA, C-5KA and KO mutants. ( B ) A 1.0 μg portion of CMV5–HA–Cidea, CMV5–Cidea–N-5KA, CMV5–Cidea–C-5KA and CMV5–Cidea-KO were co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in 60-mm-diameter dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham). Results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The value was set to 100% at zero time. The results shown are representative of the three independent experiments. ( D ) HEK-293T cells in 60-mm-diameter dishes were co-transfected with 1.0 μg of CMV5-HA control vector, CMV5–HA–Cidea (WT), CMV5–HA–Cidea–N-5KA and CMV5–HA–Cidea–C-5KA or CMV5–HA–Cidea-KO control vector with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then in vivo ubiquitination assays were performed as described above and cell lysates were subjected to IP using anti-HA in protein A/G beads. The immunoprecipitates were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. ( E ) Apoptosis induced by Cidea and its lysine-less mutants. A 1.5 μg portion of the indicated plasmids and 0.5 μg of pEGFP-N1 were co-transfected into CHO-K1 cells. Apoptotic cells were quantified, and the percentage of cell death was calculated retative to the GFP-positive cells. Results are means±S.D. for at least three independent experiments. IB, immunoblotting.

    Journal: The Biochemical Journal

    Article Title: Regulation of Cidea protein stability by the ubiquitin-mediated proteasomal degradation pathway

    doi: 10.1042/BJ20070690

    Figure Lengend Snippet: Cidea N-terminal lysine residues play a predominant role in controlling protein stability ( A ) Schematic drawing of human Cidea, WT, N-5KA, C-5KA and KO mutants. ( B ) A 1.0 μg portion of CMV5–HA–Cidea, CMV5–Cidea–N-5KA, CMV5–Cidea–C-5KA and CMV5–Cidea-KO were co-transfected with 0.5 μg of pEGFP-N1 in HEK-293T cells in 60-mm-diameter dishes using the calcium phosphate method. The CHX-based protein chase experiment was performed as described above. Total cell lysates were detected by Western blotting using anti-HA and anti-GFP antibodies. The blot shown is representative of three separate experiments. ( C ) Quantification of scanned gel bands shown in ( B ) was performed using ImageQuant-TL software (Amersham). Results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The value was set to 100% at zero time. The results shown are representative of the three independent experiments. ( D ) HEK-293T cells in 60-mm-diameter dishes were co-transfected with 1.0 μg of CMV5-HA control vector, CMV5–HA–Cidea (WT), CMV5–HA–Cidea–N-5KA and CMV5–HA–Cidea–C-5KA or CMV5–HA–Cidea-KO control vector with 0.5 μg of pXJ-40-Myc-Ub and pEGFP-N1. Then in vivo ubiquitination assays were performed as described above and cell lysates were subjected to IP using anti-HA in protein A/G beads. The immunoprecipitates were analysed by Western blotting using anti-Myc, anti-HA and anti-GFP antibodies. ( E ) Apoptosis induced by Cidea and its lysine-less mutants. A 1.5 μg portion of the indicated plasmids and 0.5 μg of pEGFP-N1 were co-transfected into CHO-K1 cells. Apoptotic cells were quantified, and the percentage of cell death was calculated retative to the GFP-positive cells. Results are means±S.D. for at least three independent experiments. IB, immunoblotting.

    Article Snippet: Transfections were performed in 60-mm-diameter dishes using either Dosper reagent (Roche) or the calcium phosphate method [ ].

    Techniques: Transfection, Western Blot, Software, Plasmid Preparation, In Vivo

    Cidea is a short-lived protein and its degradation is proteasome-dependent ( A , B and C ) A 1 μg portion of CMV5–HA–hCidea was co-transfected with 0.5 μg of pEGFP-N1 into HEK-293T, CHO-K1 and H1299 cells in 60-mm-diameter dishes using Dosper reagent. CHX-based protein chase experiments were performed as described below. At 24 h post-transfection, and 1 h prior to the addition of CHX, the medium was replaced with fresh medium, and then CHX was added to a final concentration of 100 μg/ml. Cells were harvested in 0.5 ml of lysis buffer at different time points: 0, 30, 60 and 120 min. Total cell lysates were prepared and analysed by Western blotting [IB (immunoblotting)] using anti-HA and anti-GFP as primary antibodies. The amount of GFP transiently co-expressed by pEGFP-N1 present in total cell lysates was used for the normalization of transfection protein. ( D ) Graph showing band intensities corresponding to HA–Cidea and GFP scanned and quantitified using ImageQuant-TL software (Amersham). Results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( E ) HEK-293T cells in 60-mm-diameter dishes were co-transfected with 1 μg of CMV-HA vector (lane 1) or 1 μg of CMV5–HA–hCidea (lane 2–11) and 0.5 μg of pEGFP-N1 using the calcium phosphate method. After 24 h, CHX was added to 100 μg/ml (lanes 3–11) along with different reagents [DMSO, ethanol, pepstatin (0.5 μg/ml), leupeptin (5 μg/ml), aprotinin (2 μg/ml), ALLN (50 μM), MG132 (10 μM), chloroquine (50 μg/ml) and NH 4 Cl (2.5 mM)] for 2 h. Cells were harvested in 0.5 ml of lysis buffer after the 2 h treatment. Total cell lysates were subjected to Western blotting and detected with anti-HA and anti-GFP antibodies.

    Journal: The Biochemical Journal

    Article Title: Regulation of Cidea protein stability by the ubiquitin-mediated proteasomal degradation pathway

    doi: 10.1042/BJ20070690

    Figure Lengend Snippet: Cidea is a short-lived protein and its degradation is proteasome-dependent ( A , B and C ) A 1 μg portion of CMV5–HA–hCidea was co-transfected with 0.5 μg of pEGFP-N1 into HEK-293T, CHO-K1 and H1299 cells in 60-mm-diameter dishes using Dosper reagent. CHX-based protein chase experiments were performed as described below. At 24 h post-transfection, and 1 h prior to the addition of CHX, the medium was replaced with fresh medium, and then CHX was added to a final concentration of 100 μg/ml. Cells were harvested in 0.5 ml of lysis buffer at different time points: 0, 30, 60 and 120 min. Total cell lysates were prepared and analysed by Western blotting [IB (immunoblotting)] using anti-HA and anti-GFP as primary antibodies. The amount of GFP transiently co-expressed by pEGFP-N1 present in total cell lysates was used for the normalization of transfection protein. ( D ) Graph showing band intensities corresponding to HA–Cidea and GFP scanned and quantitified using ImageQuant-TL software (Amersham). Results are expressed as the percentage of the signal of HA-Cidea compared with that of GFP. The ratio was set to 100% at zero time. The results shown are representative of three independent experiments. ( E ) HEK-293T cells in 60-mm-diameter dishes were co-transfected with 1 μg of CMV-HA vector (lane 1) or 1 μg of CMV5–HA–hCidea (lane 2–11) and 0.5 μg of pEGFP-N1 using the calcium phosphate method. After 24 h, CHX was added to 100 μg/ml (lanes 3–11) along with different reagents [DMSO, ethanol, pepstatin (0.5 μg/ml), leupeptin (5 μg/ml), aprotinin (2 μg/ml), ALLN (50 μM), MG132 (10 μM), chloroquine (50 μg/ml) and NH 4 Cl (2.5 mM)] for 2 h. Cells were harvested in 0.5 ml of lysis buffer after the 2 h treatment. Total cell lysates were subjected to Western blotting and detected with anti-HA and anti-GFP antibodies.

    Article Snippet: Transfections were performed in 60-mm-diameter dishes using either Dosper reagent (Roche) or the calcium phosphate method [ ].

    Techniques: Transfection, Concentration Assay, Lysis, Western Blot, Software, Plasmid Preparation