Journal: Molecular & Cellular Proteomics : MCP
Article Title: A Proteomic Analysis Reveals Differential Regulation of the ?S-Dependent yciGFE(katN) Locus by YncC and H-NS in Salmonella and Escherichia coli K-12
Figure Lengend Snippet: Immunodetection of σ S , His 6 -YncC and His 6 -McbR. A) Detection of His 6 -McbR and His 6 -YncC produced from p mcbR HIS and p yncC HIS , respectively. E. coli strains, harboring p yncC HIS , p mcbR HIS and the vectors pACBg and pQE30, were grown to stationary phase in LB at 37 °C and analyzed by Western blotting with anti-His antibodies. A 5-μg aliquot of total protein was loaded in each slot. 1: MC4100 yciE-lacZ (pQE30), 2: MC4100 yciE-lacZ (p yncC HIS ), 3: MC4100 yciE-lacZ (pCABg), 4: MC4100 yciE-lacZ (p mcbR HIS ). Compared with the wild-type YncC/McbR proteins, the His 6 -McbR and His 6 -YncC proteins contain 18 and 10 additional amino acids; thus, His 6 -McbR migrates more slowly on SDS-PAGE than the His 6 -YncC protein. B–D , Detection of σ S in Salmonella (STM) and E. coli K-12 (ECO) strains. Salmonella and E. coli strains in exponential phase ( D ) or stationary phase ( B , C ) in LB at 37 °C were analyzed by Western blotting with anti-σ S antibodies. 10 μg of total protein was loaded in each slot. 1: MC1061, 2: MG1655, 3: MC4100 rpoS , 4: MC4100, 5: ATCC14028, 6: ATCC rpoS , 7: ATCC hns , 8: MC4100 hns , 9: MG1655 hns , 10: MC1061 hns , 11: ATCC2922K, 12: ATCC rpoS LT2 , 13:ATCC rpoS LT2 hns , 14: ATCC2922K hns , 15: ATCC2922K rpoS .
Article Snippet: This hypothesis was ruled out because similar levels of σS were detected in MG1655, MC4100, MC1061, and ATCC14028 ( B , C , D ).
Techniques: Immunodetection, Produced, Western Blot, SDS Page