6-well plates Search Results


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  • 99
    Thermo Fisher 6 well plates
    6 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 6 well plates
    6 Well Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Corning Life Sciences 6 well plates
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 1363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    abcam 6 well plates
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    6 Well Plates, supplied by abcam, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Beyotime 6 well plates
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    6 Well Plates, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pierce streptavidin coated high capacity plates
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    Pierce Streptavidin Coated High Capacity Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore corning synthemax r surface multiwell plates
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    Corning Synthemax R Surface Multiwell Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Greiner Bio thincert cell culture inserts
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    Thincert Cell Culture Inserts, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sarstedt 6 well plates
    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) <t>6-well</t> plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P
    6 Well Plates, supplied by Sarstedt, used in various techniques. Bioz Stars score: 92/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 952 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Eppendorf AG 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    6 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 97/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher collagen coated 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    Collagen Coated 6 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    MatTek glass bottom 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    Glass Bottom 6 Well Plates, supplied by MatTek, used in various techniques. Bioz Stars score: 88/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    SPL Life Sciences 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    6 Well Plates, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore collagen coated 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    Collagen Coated 6 Well Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Corning Life Sciences matrigel coated 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    Matrigel Coated 6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    6 Well Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nest Biotechnology 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    6 Well Plates, supplied by Nest Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson collagen coated 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    Collagen Coated 6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Greiner Bio 6 well plates
    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of <t>6-well</t> plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p
    6 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor 6 well plates
    FITC-labeled PIMAP39 in mouse primary macrophages ( a-c ) and mouse liver (tissue sections; d-g ). Primary macrophage samples: cells (5 × 10 3 ) from 3-month-old mice were seeded over cover slips (22 mm) in <t>6-well</t> plates, and were treated with 500
    6 Well Plates, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    6 well plates - by Bioz Stars, 2021-01
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    92
    Iwaki America 6 well plates
    FITC-labeled PIMAP39 in mouse primary macrophages ( a-c ) and mouse liver (tissue sections; d-g ). Primary macrophage samples: cells (5 × 10 3 ) from 3-month-old mice were seeded over cover slips (22 mm) in <t>6-well</t> plates, and were treated with 500
    6 Well Plates, supplied by Iwaki America, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 39 article reviews
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    6 well plates - by Bioz Stars, 2021-01
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    N/A
    Sealing Mat for 96 Well Plates 0 5 and 1 2 mL 50 pk
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    N/A
    Cole Parmer PCR products are specifically designed for amplifying nucleic acids during the Polymerase Chain Reaction PCR process Certified RNase DNase and pyrogen safe to ensure no degradation of the
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    N/A
    The 96 Well Half Area Microplates are black polystyrene plates with a flat bottom and 96 round wells These plates have a total well volume of 190 μl and are
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    N/A
    For optimized handling of 96‑well plates with a vacuum manifold on BioRobot 9600 9604 and 3000 Qiagen MultiPROBE II Janus PerkinElmer Biomek 2000 3000 and FX NX Beckman Coulter
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    Image Search Results


    Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) 6-well plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P

    Journal: The Journal of International Medical Research

    Article Title: Titanium dioxide nanoparticles induce endoplasmic reticulum stress-mediated apoptotic cell death in liver cancer cells

    doi: 10.1177/0300060520903652

    Figure Lengend Snippet: Influence of TiO 2 nanotubes on cell growth. L02 (a) and HepG2 (b) cells were incubated in TiO 2 nanotube-coated (2.5, 5.0, 7.5, and 10 µg/mL) 6-well plates for 48 hours at 60°C. Cell numbers were counted using cell counting chambers. Cells incubated in 6-well plates coated with 0 µg/mL TiO 2 nanotubes were used as a control. * P

    Article Snippet: Solutions of TiO2 nanoparticles (400 µL) at concentrations of 0, 2.5, 5.0, 7.5, and 10 µg/mL in 75% ethanol were added to the bottom layer of 6-well plates (Costar Corning Inc., Corning, NY, USA) and incubated in an electric thermostatically controlled incubator at 60°C (DHP-9162, Shanghai Yiheng Technical Co., Ltd., Shanghai, China).

    Techniques: Incubation, Cell Counting

    L02 (a) and HepG2 (b) cells were cultured in 6-well plates for 48 hours, then apoptosis of L02 cells and HepG2 cells was detected by flow cytometry. TiO 2 increased the percentage of apoptotic liver cancer cells. Annexin V-positive cells were considered as apoptotic liver cancer cells. Cells incubated with 0 µg/mL TiO 2 were used as a control. * P

    Journal: The Journal of International Medical Research

    Article Title: Titanium dioxide nanoparticles induce endoplasmic reticulum stress-mediated apoptotic cell death in liver cancer cells

    doi: 10.1177/0300060520903652

    Figure Lengend Snippet: L02 (a) and HepG2 (b) cells were cultured in 6-well plates for 48 hours, then apoptosis of L02 cells and HepG2 cells was detected by flow cytometry. TiO 2 increased the percentage of apoptotic liver cancer cells. Annexin V-positive cells were considered as apoptotic liver cancer cells. Cells incubated with 0 µg/mL TiO 2 were used as a control. * P

    Article Snippet: Solutions of TiO2 nanoparticles (400 µL) at concentrations of 0, 2.5, 5.0, 7.5, and 10 µg/mL in 75% ethanol were added to the bottom layer of 6-well plates (Costar Corning Inc., Corning, NY, USA) and incubated in an electric thermostatically controlled incubator at 60°C (DHP-9162, Shanghai Yiheng Technical Co., Ltd., Shanghai, China).

    Techniques: Cell Culture, Flow Cytometry, Incubation

    Clonogenic and self-renewal potential of MDA-MB-231 cells cultured on a 2D surface or within Col-I gels. (A–D) Evaluation of the clonogenic potential of MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected and processed to obtain individualized cell suspension. After plating (500 cells/35 mm plate), cells were cultured for 14 days and then washed, fixed and stained with crystal violet. The images were acquired with a transilluminator and represent technical replicates of one independent experiment. (E) Bar graphs represent mean ± SD of five independent experiments performed in technical quadruplicates. (F–I) Evaluation of mammosphere (MS)-formation efficiency by MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected, processed to obtain individualized cell suspension and grown in ultra-low attachment 6-well plates (5,000 cells/well) with culture medium enriched for MS formation. After 10 days, images of at least 25 fields per well were acquired. Dashed squares outline zoomed-in MS, which have a minimum diameter of 50 μm (scale) and compact shape. Bar graphs (J) and scatter plots (K–M) represent mean ± SD of five independent experiments, performed in technical triplicate.

    Journal: PeerJ

    Article Title: High type I collagen density fails to increase breast cancer stem cell phenotype

    doi: 10.7717/peerj.9153

    Figure Lengend Snippet: Clonogenic and self-renewal potential of MDA-MB-231 cells cultured on a 2D surface or within Col-I gels. (A–D) Evaluation of the clonogenic potential of MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected and processed to obtain individualized cell suspension. After plating (500 cells/35 mm plate), cells were cultured for 14 days and then washed, fixed and stained with crystal violet. The images were acquired with a transilluminator and represent technical replicates of one independent experiment. (E) Bar graphs represent mean ± SD of five independent experiments performed in technical quadruplicates. (F–I) Evaluation of mammosphere (MS)-formation efficiency by MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected, processed to obtain individualized cell suspension and grown in ultra-low attachment 6-well plates (5,000 cells/well) with culture medium enriched for MS formation. After 10 days, images of at least 25 fields per well were acquired. Dashed squares outline zoomed-in MS, which have a minimum diameter of 50 μm (scale) and compact shape. Bar graphs (J) and scatter plots (K–M) represent mean ± SD of five independent experiments, performed in technical triplicate.

    Article Snippet: Analysis of self-renewal potential Cells previously cultured on 2D surface (5 × 103 cells/plate) or within Col-I gels (25 × 103 cells/gel) for 7 days were collected, dissociated, and cell suspension was plated in ultra-low attachment 6-well plates (Corning Inc., Corning, NY, USA) at low cell density (5 × 103 cells/well) in DMEM-F12 containing 20 ng/ml bFGF (Sigma, Ronkonkoma, NY, USA), 20 ng/ml EGF (Sigma, Ronkonkoma, NY, USA) and 1X B-27 supplement (Gibco, Langley, OK, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture, Staining

    Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of 6-well plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p

    Journal: Cells

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

    doi: 10.3390/cells9071715

    Figure Lengend Snippet: Immunobiotic mediated regulation of adipocyte differentiation in an immune cell-adipocyte co-culture model. The mRNA expressions of PPARγ and GLUT4 resulted from various immunobiotic lactic acid bacteria (LAB) stimulation into the co-culture model. A total of 1 × 10 6 immune cells/well of 6-well plate were cultured on adipocytes 4-days differentiated. LAB samples (5 × 10 8 cells/well) also added into the adipocyte plate and maintained for further 2 or 4 Days. All data shown are the average ±SD of 3 independent experiments performed in triplicate. The asterisk (*) indicated statistical differences when compared with control at the significance level of p

    Article Snippet: Induction of Fat Accumulation by SFAs Stimulation Adipocytes were cultured at a density of 2.5 × 104 /cm2 in 6-well plates (BD Falcon, Tokyo, Japan).

    Techniques: Co-Culture Assay, Cell Culture

    Immunobiotic mediated inflammatory responses in the antigen presenting cells (APCs) of Payer’s patches. The APCs were seeded at 1 × 10 6 cells/well of 6-well plate and cultured with each bacterial strain (5 × 10 8 cells/well) separately for 24 h and the mRNA expressions of IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IFN-β and IFN-γ) were analyzed with RT-qPCR. Data shown are the mean ±SD of 3 independent experiments performed in triplicates. The asterisks: (*) and (**) indicated statistical differences with significant levels of p

    Journal: Cells

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

    doi: 10.3390/cells9071715

    Figure Lengend Snippet: Immunobiotic mediated inflammatory responses in the antigen presenting cells (APCs) of Payer’s patches. The APCs were seeded at 1 × 10 6 cells/well of 6-well plate and cultured with each bacterial strain (5 × 10 8 cells/well) separately for 24 h and the mRNA expressions of IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IFN-β and IFN-γ) were analyzed with RT-qPCR. Data shown are the mean ±SD of 3 independent experiments performed in triplicates. The asterisks: (*) and (**) indicated statistical differences with significant levels of p

    Article Snippet: Induction of Fat Accumulation by SFAs Stimulation Adipocytes were cultured at a density of 2.5 × 104 /cm2 in 6-well plates (BD Falcon, Tokyo, Japan).

    Techniques: Cell Culture, Quantitative RT-PCR

    Immunobiotic mediated regulation of fat accumulation and inflammatory responses in an immune cell-adipocyte co-culture model. A total of 1 × 10 6 immune cells/well of a 6-well plate were cultured on adipocytes 4-days differentiated. Bacterial samples (5 × 10 8 cells/well) were also added and cultured for 2 or 4 days. Adipocyte cells were stained with Oil red O stained and fat accumulation in the adipocytes was quantitatively analyzed by image J software. The ELISA-based concentration of CCL2 protein in supernatant of adipocyte-culture stimulated by immune cells and lactic acid bacteria (LAB). All data shown are the mean ± SD of three independent experiments performed in triplicate. The asterisks: ‘*’ and ‘**’ indicated statistical differences either between bars of panel-a and control or between bars of panel-b and IMU, with significant levels of p

    Journal: Cells

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

    doi: 10.3390/cells9071715

    Figure Lengend Snippet: Immunobiotic mediated regulation of fat accumulation and inflammatory responses in an immune cell-adipocyte co-culture model. A total of 1 × 10 6 immune cells/well of a 6-well plate were cultured on adipocytes 4-days differentiated. Bacterial samples (5 × 10 8 cells/well) were also added and cultured for 2 or 4 days. Adipocyte cells were stained with Oil red O stained and fat accumulation in the adipocytes was quantitatively analyzed by image J software. The ELISA-based concentration of CCL2 protein in supernatant of adipocyte-culture stimulated by immune cells and lactic acid bacteria (LAB). All data shown are the mean ± SD of three independent experiments performed in triplicate. The asterisks: ‘*’ and ‘**’ indicated statistical differences either between bars of panel-a and control or between bars of panel-b and IMU, with significant levels of p

    Article Snippet: Induction of Fat Accumulation by SFAs Stimulation Adipocytes were cultured at a density of 2.5 × 104 /cm2 in 6-well plates (BD Falcon, Tokyo, Japan).

    Techniques: Co-Culture Assay, Cell Culture, Staining, Software, Enzyme-linked Immunosorbent Assay, Concentration Assay

    FITC-labeled PIMAP39 in mouse primary macrophages ( a-c ) and mouse liver (tissue sections; d-g ). Primary macrophage samples: cells (5 × 10 3 ) from 3-month-old mice were seeded over cover slips (22 mm) in 6-well plates, and were treated with 500

    Journal:

    Article Title: A PTP4A3 Peptide PIMAP39 Modulates TNF-Alpha Levels and Endotoxic Shock

    doi: 10.1159/000235685

    Figure Lengend Snippet: FITC-labeled PIMAP39 in mouse primary macrophages ( a-c ) and mouse liver (tissue sections; d-g ). Primary macrophage samples: cells (5 × 10 3 ) from 3-month-old mice were seeded over cover slips (22 mm) in 6-well plates, and were treated with 500

    Article Snippet: For macrophage samples, cells (5 × 103 ) from 3-month-old mice were seeded over cover slips (22 mm; VWR Scientific) in 6-well plates at 37°C and 5% CO2 overnight.

    Techniques: Labeling, Mouse Assay