6-well plates Search Results


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  • 92
    Corning Life Sciences 6 well plates
    (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the <t>6-well</t> plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.
    6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 1350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Greiner Bio 6 well plates
    (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the <t>6-well</t> plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.
    6 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Sarstedt 6 well plates
    (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the <t>6-well</t> plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.
    6 Well Plates, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson 6 well plates
    (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the <t>6-well</t> plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.
    6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa 6 well plates
    (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the <t>6-well</t> plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.
    6 Well Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    6 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 6901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Boster Bio 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    6 Well Plates, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Elscolab 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    6 Well Plates, supplied by Elscolab, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Nest Biotechnology 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    6 Well Plates, supplied by Nest Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SPL Life Sciences 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    6 Well Plates, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson primaria 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    Primaria 6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Eppendorf AG 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    6 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Flexcell International Corp bioflex 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    Bioflex 6 Well Plates, supplied by Flexcell International Corp, used in various techniques. Bioz Stars score: 89/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Corning Life Sciences attachment 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    Attachment 6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Greiner Bio coated 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    Coated 6 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson falcon 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    Falcon 6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SPL Life Sciences sterile 6 well plates
    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a <t>6-well</t> plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.
    Sterile 6 Well Plates, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Corning Life Sciences transwell 6 well plates
    Figure 4.  BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.
    Transwell 6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher upcell 6 well plates
    Figure 4.  BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.
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    Image Search Results


    (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the 6-well plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.

    Journal: Regenerative Therapy

    Article Title: Effect of mechanical vibration stress in cell culture on human induced pluripotent stem cells

    doi: 10.1016/j.reth.2019.05.002

    Figure Lengend Snippet: (A) Schematic illustration of mechanical vibration experiment in this work. Vertical mechanical vibration was applied to the 6-well plate for 10 min every 24 h after the medium change. Phase-contrast microscopic images were acquired every 12 h. Cells were passaged continuously from P3 (Cycle 1) to P5 (Cycle 3). On the 7th day, the samples treated with vibration were collected, and seeded into a new 6-well plate. At the same time, partial samples were stained to evaluate the undifferentiation marker (B) Visualization of relative distances between designed conditions in this work. Four related parameters (acceleration, frequency, amplitude, energy) are visualized by principal component analysis. The proportion of variance for each principal component (PC) is PC1 (0.56), PC2 (0.27), and PC3 (0.16). The loadings are: acceleration: frequency: amplitude: energy = PC1 (−0.04, 0.42, −0.65, −0.64), PC2 (0.89, −0.43, 0.09, 0.13), PC3 (0.46, −0.80, −0.25, −0.30). The color representation is visualized in the legend. Representative four points from tapping and closing of incubator door condition measured in the preliminary examination is visualized with smaller dots to indicate the range of vibration.

    Article Snippet: Cells dissociated into single cells were seeded at the concentration of 5000 cells/well in 6-well plates (Corning Life Sciences, Corning, NY, USA).

    Techniques: Staining, Marker

    Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a 6-well plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.

    Journal: Virology

    Article Title: A 2a/1b full-length p7 inter-genotypic chimeric genome of hepatitis C virus is infectious in vitro

    doi: 10.1016/j.virol.2006.10.014

    Figure Lengend Snippet: Immunoblot analysis of the cells transfected with the wild type and chimeric genomes or infected with the wild type and chimeric viruses. The same number of cells was used in the transfection and infection experiments. 150 μl of Laemmli buffer was added to each well of a 6-well plate, and 12 μl of each sample was analyzed onto a 10% SDS-PAGE gel. The monoclonal antibody AP33 was used to detect the E2 protein, 1: mock-transfected cells, 2: JFH1-transfected cells, 3: Pp7-transfected cells, 4: Fp7-transfected cells, 5: JFH1 infected cells, 6: Pp7 infected cells, 7: Fp7 infected cells.

    Article Snippet: To examine the replication competency of the recombinant genomes, 4 μg of in vitro generated RNA transcripts was transfected into cells in 6-well plates (Nalge Nunc, Rochester, NY) using 10 μl of DMRIE-C (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.

    Techniques: Transfection, Infection, SDS Page

    Figure 4.  BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.

    Journal: Cell Cycle

    Article Title: Therapeutic targeting of Polo-like kinase-1 and Aurora kinases in T-cell acute lymphoblastic leukemia

    doi: 10.4161/cc.29267

    Figure Lengend Snippet: Figure 4. BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ® 6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ® 6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.

    Article Snippet: In another set of experiments, MS-5 cells were grown in the lower chamber of Transwell® 6-well plates (Corning) containing a 0.4-µm polyester membrane, then MOLT-4 cells were added to the upper chamber and treated with BI6727 (40 nM).

    Techniques: Mass Spectrometry, MTT Assay, Western Blot, Binding Assay, Flow Cytometry, Cytometry