6 fam Millipore Search Results


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  • 77
    Millipore 6 fam se
    6 Fam Se, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Millipore 6 fam labeled primer
    6 Fam Labeled Primer, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore 6 fam fluorescein molecules
    6 Fam Fluorescein Molecules, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Millipore 6 fam sc1 rna
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    6 Fam Sc1 Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore 6 fam bhq 1 hydrolysis probe
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    6 Fam Bhq 1 Hydrolysis Probe, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore reporter dye 6 carboxyfluorescein
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    Reporter Dye 6 Carboxyfluorescein, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 6 carboxyfluorescein
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    6 Carboxyfluorescein, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore lna target probe
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    Lna Target Probe, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore rox dyes
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    Rox Dyes, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore 2 ned fluorescent dye
    Arginine perturbations disrupt FMRP LCR <t>-sc1</t> <t>RNA</t> phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with <t>6-FAM–labeled</t> sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.
    2 Ned Fluorescent Dye, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Arginine perturbations disrupt FMRP LCR -sc1 RNA phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with 6-FAM–labeled sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Phosphoregulated FMRP phase separation models activity-dependent translation through bidirectional control of mRNA granule formation

    doi: 10.1073/pnas.1814385116

    Figure Lengend Snippet: Arginine perturbations disrupt FMRP LCR -sc1 RNA phase separation but do not notably affect FMRP LCR binding to sc-1 RNA. ( A ) Schematic illustration of the three constructs used. ( B ) Similar binding affinities for interactions between FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR with 6-FAM–labeled sc1 RNA (25 nM) are shown using FP assayed in 25 mM Na 2 PO 4 , pH 7.4, 100 mM KCl, 2 mM DTT, 0.02 mg/mL E. coli tRNA, and 0.01% Nonidet P-40. Apparent dissociation constants ( k d,app ) from three experimental replicates. ( C ) Phase diagram of FMRP LCR , Me-FMRP LCR , and RtoK-FMRP LCR at a constant protein concentration of 100 μM with increasing sc1 RNA concentrations in buffer containing 25 mM Na 2 PO 4 , pH 7.4, 30 mM KCl, and 2 mM DTT. Green circles represent an observation of droplet formation, and open circles represent no droplet formation detected. The dotted red line is a visual aid for a qualitative phase boundary of FMRP LCR. ( Right ) Representative DIC microscopy images of droplets for the addition of 2.5 μM of RNA in the conditions described. (Scale bar, 5 μm.) Phase-separation propensity is dramatically decreased for methylated and RtoK mutant proteins, with smaller droplet sizes observed.

    Article Snippet: Sc1 RNA, 5′-labeled Cy3-sc1 RNA, 5′-labeled Cy3-miRNA-125b, and 5′-labeled 6-FAM-sc1 RNA were purchased from Sigma as lyophilized samples.

    Techniques: Binding Assay, Construct, Labeling, Protein Concentration, Microscopy, Methylation, Mutagenesis