5x mmulv buffer Search Results


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  • 99
    New England Biolabs moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5x reaction buffer
    5x Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcriptase rt buffer
    Reverse Transcriptase Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5x rt buffer
    5x Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase inhibitor
    Rnase Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mmulv reverse transcriptase
    Mmulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher aidtm mmulv h reverse transcriptase
    Aidtm Mmulv H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ribonuclease inhibitor
    Ribonuclease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dntp mix
    Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14684 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt primers
    Oligo Dt Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher moloney murine leukemia virus
    Moloney Murine Leukemia Virus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nuclease free water
    Nuclease Free Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher molony murine leukemia virus reverse transcriptase enzyme
    Molony Murine Leukemia Virus Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche high pure viral rna isolation kit
    High Pure Viral Rna Isolation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher revertaid reverse transcriptase kit
    Revertaid Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mmulv rt
    Mmulv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG eppendorf mastercycler
    Eppendorf Mastercycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 4823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs rnase h
    Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. <t>RNase</t> H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a T7 RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.
    Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t7 rnap
    Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a <t>T7</t> RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.
    T7 Rnap, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biometra real time qpcr cdna
    Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a <t>T7</t> RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.
    Real Time Qpcr Cdna, supplied by Biometra, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bsa molecular biology grade
    Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a <t>T7</t> RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.
    Bsa Molecular Biology Grade, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a T7 RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.

    Journal: bioRxiv

    Article Title: Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout

    doi: 10.1101/2020.02.18.954719

    Figure Lengend Snippet: Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a T7 RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.

    Article Snippet: An enzyme mix was prepared containing T7 RNAP, Moloney Murine Leukemia Virus reverse transcriptase (mMuLV), RNase H (New England Biolabs Inc., M0297) and bovine serum albumin (New England Biolabs Inc., B9000S).

    Techniques: Amplification, Sequencing, Construct, Generated

    Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a T7 RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.

    Journal: bioRxiv

    Article Title: Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout

    doi: 10.1101/2020.02.18.954719

    Figure Lengend Snippet: Apta-NASBA reaction. Schematic of the reaction. 1) Promoter-containing primer ( PP ) binds the amplicon of interest. Reverse transcriptase ( RT ) extends PP , generating a DNA-RNA hybrid comprised of the amplicon target sequence ( TS ) and an antisense DNA copy of the amplicon with an overhang containing the T7 RNA polymerase (T7 RNAP) promoter. RNase H degrades the original amplicon RNA of interest. Coding primer ( CP ) binds. RT extends CP , generating a DNA duplex corresponding to the amplicon, with a T7 RNAP promoter (red) and an aptamer coding sequence (blue). T7 RNAP generates an RNA fusion construct that contains the sequence antisense to TS , fused at the 3′ end with the aptamer corresponding to the sequence in CP (green). CP binds this RNA, which again enters the RT-RNase H-T7 RNAP cycle. At each step, RNA is generated with multiple turnover, enabling exponential amplification. Images on the right are representative Apta-NASBA reactions using aptamers that span the fluorescent spectrum.

    Article Snippet: An enzyme mix was prepared containing T7 RNAP, Moloney Murine Leukemia Virus reverse transcriptase (mMuLV), RNase H (New England Biolabs Inc., M0297) and bovine serum albumin (New England Biolabs Inc., B9000S).

    Techniques: Amplification, Sequencing, Construct, Generated