5x first strand buffer Thermo Fisher Search Results


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  • 90
    Thermo Fisher 5x second strand synthesis buffer
    5x Second Strand Synthesis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5x first strand buffer
    5x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5x first strand fs buffer
    5x First Strand Fs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript first strand buffer
    Superscript First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript ii 5x first strand buffer
    Superscript Ii 5x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher revertaid first strand cdna synthesis kit
    Revertaid First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 29432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase free 5x first strand
    Rnase Free 5x First Strand, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher first strand synthesis buffer
    First Strand Synthesis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher buffer
    Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher superscript iii reverse transcriptase
    Microcapillary electrophoresis of total <t>RNA</t> from whole cells and projections. Microcapillary electrophoresis of total RNA (Bioanalyzer RNA pico assay) from whole cells and projections of <t>three</t> biological replicates. Only RNAs > ~150 nt were sequenced for this study. In projections, the band at ~100 nt was striking. By sequencing RNAs within 20–150 nt size range, we found that they were mostly full length and fragmented tRNAs (unpublished data).
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 69294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher lysis buffer
    Microcapillary electrophoresis of total <t>RNA</t> from whole cells and projections. Microcapillary electrophoresis of total RNA (Bioanalyzer RNA pico assay) from whole cells and projections of <t>three</t> biological replicates. Only RNAs > ~150 nt were sequenced for this study. In projections, the band at ~100 nt was striking. By sequencing RNAs within 20–150 nt size range, we found that they were mostly full length and fragmented tRNAs (unpublished data).
    Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 35040 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher m mlv reverse transcriptase buffer
    Microcapillary electrophoresis of total <t>RNA</t> from whole cells and projections. Microcapillary electrophoresis of total RNA (Bioanalyzer RNA pico assay) from whole cells and projections of <t>three</t> biological replicates. Only RNAs > ~150 nt were sequenced for this study. In projections, the band at ~100 nt was striking. By sequencing RNAs within 20–150 nt size range, we found that they were mostly full length and fragmented tRNAs (unpublished data).
    M Mlv Reverse Transcriptase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dtt
    Microcapillary electrophoresis of total <t>RNA</t> from whole cells and projections. Microcapillary electrophoresis of total RNA (Bioanalyzer RNA pico assay) from whole cells and projections of <t>three</t> biological replicates. Only RNAs > ~150 nt were sequenced for this study. In projections, the band at ~100 nt was striking. By sequencing RNAs within 20–150 nt size range, we found that they were mostly full length and fragmented tRNAs (unpublished data).
    Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 23611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Microcapillary electrophoresis of total RNA from whole cells and projections. Microcapillary electrophoresis of total RNA (Bioanalyzer RNA pico assay) from whole cells and projections of three biological replicates. Only RNAs > ~150 nt were sequenced for this study. In projections, the band at ~100 nt was striking. By sequencing RNAs within 20–150 nt size range, we found that they were mostly full length and fragmented tRNAs (unpublished data).

    Journal: eLife

    Article Title: Free circular introns with an unusual branchpoint in neuronal projections

    doi: 10.7554/eLife.47809

    Figure Lengend Snippet: Microcapillary electrophoresis of total RNA from whole cells and projections. Microcapillary electrophoresis of total RNA (Bioanalyzer RNA pico assay) from whole cells and projections of three biological replicates. Only RNAs > ~150 nt were sequenced for this study. In projections, the band at ~100 nt was striking. By sequencing RNAs within 20–150 nt size range, we found that they were mostly full length and fragmented tRNAs (unpublished data).

    Article Snippet: RNA was hydrolyzed using 5X first strand buffer (provided with Superscript III reverse transcriptase, ThermoFisher #18080044) at 94°C for 4 min and 50 s and immediately moved to ice.

    Techniques: Electrophoresis, Sequencing