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    Novus Biologicals pht1 slc15a4
    Primers for qRT-PCR.
    Pht1 Slc15a4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pht1 slc15a4/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pht1 slc15a4 - by Bioz Stars, 2024-02
    90/100 stars
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    Standard format Plasmid sent in bacteria as agar stab
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    Primers for qRT-PCR.

    Journal: Frontiers in Physiology

    Article Title: Carnosine Supplementation Enhances Post Ischemic Hind Limb Revascularization

    doi: 10.3389/fphys.2019.00751

    Figure Lengend Snippet: Primers for qRT-PCR.

    Article Snippet: The following critical reagents were purchased from commercial vendors: carnosine, hematoxylin, and eosin dyes, histamine dihydrochloride, EDC [ N -(3-Dimethylaminopropyl)- N ′-ethylcarbodiimide], and N -methylmorpholine (Sigma-Aldrich); dry DCM and dry DMF (Acros Organics), microfil dye (Flow Tech Inc.); isolectin antibody (Molecular Probes); antibodies to PEPT2/SCL15A2 (ab 186999; abcam); HIF-1α (Sc-13515; Santa Cruz), and PHT1/SLC15A4 (NB1-87279; Novus biological), HDAC (05-814; Millipore), and VEGF (Sc-7269; Santa Cruz).

    Techniques:

    Carnosine transport is enhanced in the hamstring skeletal muscle during hind limb ischemia (HLI). (A) Representative LC/MS/MS spectra of carnosine ( m/z 227) and internal standard ( m/z 319) detected in the hamstring skeletal muscle. (B) Quantitative analysis of carnosine in the hamstring skeletal muscle normalized to total protein. (C) qRT PCR shows the mRNA levels of Atpgd1, Cndp2, Pht1, TauT, and Pept2 in the hamstring skeletal muscle after 3 days of sham and HLI surgeries without and with carnosine. (D) (i) Representative Western blots of PHT1 and PEPT2 normalized to amido-black (AB) in the ischemic limb of carnosine treated and untreated mice 3 days after HLI, (ii) lower panel shows the quantitative analysis for PHT1 and PEPT2. Data are mean ± SEM, n = 3–5 mice in each group, ∗ p < 0.05 vs. sham, δ p < 0.05 vs. sham + carnosine, # p < 0.05 vs. untreated HLI.

    Journal: Frontiers in Physiology

    Article Title: Carnosine Supplementation Enhances Post Ischemic Hind Limb Revascularization

    doi: 10.3389/fphys.2019.00751

    Figure Lengend Snippet: Carnosine transport is enhanced in the hamstring skeletal muscle during hind limb ischemia (HLI). (A) Representative LC/MS/MS spectra of carnosine ( m/z 227) and internal standard ( m/z 319) detected in the hamstring skeletal muscle. (B) Quantitative analysis of carnosine in the hamstring skeletal muscle normalized to total protein. (C) qRT PCR shows the mRNA levels of Atpgd1, Cndp2, Pht1, TauT, and Pept2 in the hamstring skeletal muscle after 3 days of sham and HLI surgeries without and with carnosine. (D) (i) Representative Western blots of PHT1 and PEPT2 normalized to amido-black (AB) in the ischemic limb of carnosine treated and untreated mice 3 days after HLI, (ii) lower panel shows the quantitative analysis for PHT1 and PEPT2. Data are mean ± SEM, n = 3–5 mice in each group, ∗ p < 0.05 vs. sham, δ p < 0.05 vs. sham + carnosine, # p < 0.05 vs. untreated HLI.

    Article Snippet: The following critical reagents were purchased from commercial vendors: carnosine, hematoxylin, and eosin dyes, histamine dihydrochloride, EDC [ N -(3-Dimethylaminopropyl)- N ′-ethylcarbodiimide], and N -methylmorpholine (Sigma-Aldrich); dry DCM and dry DMF (Acros Organics), microfil dye (Flow Tech Inc.); isolectin antibody (Molecular Probes); antibodies to PEPT2/SCL15A2 (ab 186999; abcam); HIF-1α (Sc-13515; Santa Cruz), and PHT1/SLC15A4 (NB1-87279; Novus biological), HDAC (05-814; Millipore), and VEGF (Sc-7269; Santa Cruz).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Quantitative RT-PCR, Western Blot

    Post ischemic angiogenic signaling in the ischemic muscle is potentiated by carnosine feeding. Bioavailability of carnosine in the ischemic limb is facilitated by increased expression of carnosine transporters (human peptide transporter; PEPT2, carnosine/histidine transporter; PHT1) and normalization of taurine (TauT) transporters. Chelation of (iron) Fe 2+ by carnosine could inactivate prolyl hydroxylase domain proteins (PHDs), stabilize and translocate hypoxia inducible factor-1 alpha (HIF-1α) to the nucleus, stimulate vascular endothelial growth factor (VEGF) release and mobilize Flk-1 + /Sca-1 + cells into circulation.

    Journal: Frontiers in Physiology

    Article Title: Carnosine Supplementation Enhances Post Ischemic Hind Limb Revascularization

    doi: 10.3389/fphys.2019.00751

    Figure Lengend Snippet: Post ischemic angiogenic signaling in the ischemic muscle is potentiated by carnosine feeding. Bioavailability of carnosine in the ischemic limb is facilitated by increased expression of carnosine transporters (human peptide transporter; PEPT2, carnosine/histidine transporter; PHT1) and normalization of taurine (TauT) transporters. Chelation of (iron) Fe 2+ by carnosine could inactivate prolyl hydroxylase domain proteins (PHDs), stabilize and translocate hypoxia inducible factor-1 alpha (HIF-1α) to the nucleus, stimulate vascular endothelial growth factor (VEGF) release and mobilize Flk-1 + /Sca-1 + cells into circulation.

    Article Snippet: The following critical reagents were purchased from commercial vendors: carnosine, hematoxylin, and eosin dyes, histamine dihydrochloride, EDC [ N -(3-Dimethylaminopropyl)- N ′-ethylcarbodiimide], and N -methylmorpholine (Sigma-Aldrich); dry DCM and dry DMF (Acros Organics), microfil dye (Flow Tech Inc.); isolectin antibody (Molecular Probes); antibodies to PEPT2/SCL15A2 (ab 186999; abcam); HIF-1α (Sc-13515; Santa Cruz), and PHT1/SLC15A4 (NB1-87279; Novus biological), HDAC (05-814; Millipore), and VEGF (Sc-7269; Santa Cruz).

    Techniques: Expressing