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    Cell Signaling Technology Inc fnip2
    A. UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were assayed for mTORC1-dependent phosphorylation of S6 and mTORC2-dependent phosphorylation of AKT(S473) by western blotting. B. Extracts from UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were immunoprecipitated with anti-FLCN antibody. The levels of FLCN, FNIP1 and <t>FNIP2</t> in the precipitates (α-FLCN IP) and extracts (Extract) were determined by western blotting. C. Extracts from UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were immunoprecipitated with anti-RagC antibody. The levels of FLCN, FNIP2, RagC in the precipitates (α-RagC IP) and extracts (Extract) were assayed by western blotting.
    Fnip2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fnip2/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fnip2 - by Bioz Stars, 2024-02
    92/100 stars
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    Standard format Plasmid sent in bacteria as agar stab
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    A. UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were assayed for mTORC1-dependent phosphorylation of S6 and mTORC2-dependent phosphorylation of AKT(S473) by western blotting. B. Extracts from UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were immunoprecipitated with anti-FLCN antibody. The levels of FLCN, FNIP1 and FNIP2 in the precipitates (α-FLCN IP) and extracts (Extract) were determined by western blotting. C. Extracts from UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were immunoprecipitated with anti-RagC antibody. The levels of FLCN, FNIP2, RagC in the precipitates (α-RagC IP) and extracts (Extract) were assayed by western blotting.

    Journal: FEBS letters

    Article Title: Ciliary localization of folliculin mediated via a kinesin-2-binding motif is required for its functions in mTOR regulation and tumor suppression

    doi: 10.1002/1873-3468.13959

    Figure Lengend Snippet: A. UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were assayed for mTORC1-dependent phosphorylation of S6 and mTORC2-dependent phosphorylation of AKT(S473) by western blotting. B. Extracts from UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were immunoprecipitated with anti-FLCN antibody. The levels of FLCN, FNIP1 and FNIP2 in the precipitates (α-FLCN IP) and extracts (Extract) were determined by western blotting. C. Extracts from UOK257 cells stably expressing control vector (Ctrl), wild type and mutants of FLCN were immunoprecipitated with anti-RagC antibody. The levels of FLCN, FNIP2, RagC in the precipitates (α-RagC IP) and extracts (Extract) were assayed by western blotting.

    Article Snippet: Antibodies for FLCN (Cat# 3697), FNIP2 (Cat# 57612), AKT (Cat# 9272), phospho-AKT at S473 (cat# 4051), S6 (#2317) and phospho-S6 at S235/236 (Cat# 2211), 4EBP1 (#9452) and phospho-4EBP1at S65 (#9451), RagC (#9480) antibodies were purchased from Cell Signaling Technology.

    Techniques: Stable Transfection, Expressing, Plasmid Preparation, Western Blot, Immunoprecipitation