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    Novus Biologicals anti sos2
    mRNA expression of Ras-GEFs and Ras isoforms in IEC-18 cells (revealed by RNA-Seq analysis)
    Anti Sos2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sos2/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti sos2 - by Bioz Stars, 2024-02
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    mRNA expression of Ras-GEFs and Ras isoforms in IEC-18 cells (revealed by RNA-Seq analysis)

    Journal: The Journal of Biological Chemistry

    Article Title: A novel antiproliferative PKCα-Ras-ERK signaling axis in intestinal epithelial cells

    doi: 10.1016/j.jbc.2022.102121

    Figure Lengend Snippet: mRNA expression of Ras-GEFs and Ras isoforms in IEC-18 cells (revealed by RNA-Seq analysis)

    Article Snippet: Anti-KSR1 (ab68483) and anti-PKCα (ab32376 used for immunoblot analysis; ab221611 used for immunoprecipitation) antibodies were from Abcam; anti-H-Ras (A19619), anti–pan-Ras (A4735), anti-RasGRP1 (A10495), and anti-PKCε (A2110) antibodies were from Abclonal; anti-KSR2 (H00283455-M08) antibody was from Abnova; anti-A-Raf (75804S), anti-B-Raf (14814S), anti–phospho-Ser259 C-Raf (9421), anti–phospho-Ser338 C-Raf (9427), anti-cyclin D1 (2978), anti-Gapdh (5174), anti–pan-Ras (3339S), anti-pERK (9106 and 4370), anti-pMEK (9121), anti–total ERK (9102), anti–total MEK (9122), anti-SOS1 (D3T7T), and anti-pRSK(S380) (9341) antibodies were from Cell Signaling Technology; anti-C-Raf (610151) and anti-p21 Cip1 (556430) antibodies were from BD Pharma; anti-Id1 (BCH-1/195-14-50) antibody was from BioCheck; anti-pRSK(S380) (04-418) was from Millipore; anti-p21 Cip1 (NBP2-29463) antibody was from Novus Biologicals; anti-RasGRP3 (sc-271068), anti-SOS2 (sc-393667), and anti-PKCδ (sc-8402) antibodies were from Santa Cruz Biotechnology; and anti-actin (A2066) antibody was from Sigma–Aldrich.

    Techniques: Expressing

    Role of SOS1/2 RasGEFs in PKCα-induced growth inhibitory ERK signaling. A and B , IEC-18 cells were transfected with nontargeting (NT) siRNA or siRNA targeting SOS1 and/or SOS2 as indicated. After 48 h, cells were treated with 100 nM PMA for 15 min ( A ) or 2 h ( B ) and analyzed for the indicated proteins by Western blotting. The graph to the right of the blots in ( A ) shows densitometric analysis of relative levels of pERK normalized to loading control (±SD, n = 3. ∗ p ≤ 0.05; ∗∗ p ≤ 0.01). All data in ( B ) are from single immunoblots; the vertical lines indicate rearrangement of the blot for clarity. C , i, IEC-18 cells were pretreated with vehicle (−) or 4 μM Gö6976 to inhibit PKCα activity, followed by 15 min treatment with vehicle (−) or 100 nM PMA as indicated. Lysates were run on Phos-tag gels and analyzed for SOS1 by immunoblotting. ii, IEC-18 cells were treated with 100 nM PMA for 15 min. Cells were then lysed, and half of each lysate was treated with lambda phosphatase (λPP) as indicated before being subjected to Phos-tag gel analysis. iii, Phos-tag gel analysis of cells treated with PMA in the absence or presence of the ERK inhibitor SCH772984 as indicated. iv, as in ( A ), except that (NT) siRNA or SOS1 siRNA transfected cells were treated as indicated for 2 h. Data are representative of at least three independent experiments, except C (ii) and C (iii) which are representative of two independent experiments. PMA, phorbol 12-myristate 13-acetate.

    Journal: The Journal of Biological Chemistry

    Article Title: A novel antiproliferative PKCα-Ras-ERK signaling axis in intestinal epithelial cells

    doi: 10.1016/j.jbc.2022.102121

    Figure Lengend Snippet: Role of SOS1/2 RasGEFs in PKCα-induced growth inhibitory ERK signaling. A and B , IEC-18 cells were transfected with nontargeting (NT) siRNA or siRNA targeting SOS1 and/or SOS2 as indicated. After 48 h, cells were treated with 100 nM PMA for 15 min ( A ) or 2 h ( B ) and analyzed for the indicated proteins by Western blotting. The graph to the right of the blots in ( A ) shows densitometric analysis of relative levels of pERK normalized to loading control (±SD, n = 3. ∗ p ≤ 0.05; ∗∗ p ≤ 0.01). All data in ( B ) are from single immunoblots; the vertical lines indicate rearrangement of the blot for clarity. C , i, IEC-18 cells were pretreated with vehicle (−) or 4 μM Gö6976 to inhibit PKCα activity, followed by 15 min treatment with vehicle (−) or 100 nM PMA as indicated. Lysates were run on Phos-tag gels and analyzed for SOS1 by immunoblotting. ii, IEC-18 cells were treated with 100 nM PMA for 15 min. Cells were then lysed, and half of each lysate was treated with lambda phosphatase (λPP) as indicated before being subjected to Phos-tag gel analysis. iii, Phos-tag gel analysis of cells treated with PMA in the absence or presence of the ERK inhibitor SCH772984 as indicated. iv, as in ( A ), except that (NT) siRNA or SOS1 siRNA transfected cells were treated as indicated for 2 h. Data are representative of at least three independent experiments, except C (ii) and C (iii) which are representative of two independent experiments. PMA, phorbol 12-myristate 13-acetate.

    Article Snippet: Anti-KSR1 (ab68483) and anti-PKCα (ab32376 used for immunoblot analysis; ab221611 used for immunoprecipitation) antibodies were from Abcam; anti-H-Ras (A19619), anti–pan-Ras (A4735), anti-RasGRP1 (A10495), and anti-PKCε (A2110) antibodies were from Abclonal; anti-KSR2 (H00283455-M08) antibody was from Abnova; anti-A-Raf (75804S), anti-B-Raf (14814S), anti–phospho-Ser259 C-Raf (9421), anti–phospho-Ser338 C-Raf (9427), anti-cyclin D1 (2978), anti-Gapdh (5174), anti–pan-Ras (3339S), anti-pERK (9106 and 4370), anti-pMEK (9121), anti–total ERK (9102), anti–total MEK (9122), anti-SOS1 (D3T7T), and anti-pRSK(S380) (9341) antibodies were from Cell Signaling Technology; anti-C-Raf (610151) and anti-p21 Cip1 (556430) antibodies were from BD Pharma; anti-Id1 (BCH-1/195-14-50) antibody was from BioCheck; anti-pRSK(S380) (04-418) was from Millipore; anti-p21 Cip1 (NBP2-29463) antibody was from Novus Biologicals; anti-RasGRP3 (sc-271068), anti-SOS2 (sc-393667), and anti-PKCδ (sc-8402) antibodies were from Santa Cruz Biotechnology; and anti-actin (A2066) antibody was from Sigma–Aldrich.

    Techniques: Transfection, Western Blot, Activity Assay