DSMZ
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DSMZ
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Millipore
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Bruker Corporation
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Image Search Results

Journal: Data in Brief
Article Title: Data on the optimization of an archaea-specific probe-based qPCR assay
doi: 10.1016/j.dib.2020.106610
Figure Lengend Snippet: The bacterial and archaeal strains used as template for optimizing the archaeal qPCR assay. The genomic DNA concentration of all strains was set to a working concentration of 200 pg/µL. The qPCR efficiency was further tested using amplicons of the archaeal 16S rRNA genes. The targeted DNA concentrations of the prepared amplicons were 10 pg/µL, but ranged in general between 8 and 11 pg/µL, except for DSM 800 M. barkerii , for which the amplicon DNA concentration was lower. The qPCR was run using two different dilutions of the amplicons.
Article Snippet:
Techniques: Concentration Assay, Amplification, DNA Extraction

Journal: Data in Brief
Article Title: Data on the optimization of an archaea-specific probe-based qPCR assay
doi: 10.1016/j.dib.2020.106610
Figure Lengend Snippet: The average Crossing point (Cp) values for the original genomic DNA extracts, negative DNA extraction kit controls and no template controls in the bacterial and archaeal 16S rRNA gene targeted Sybr green assays, respectively. Archaeal DNA extracts were not tested in the bacterial assay (na, not analysed). – indicates negative result.
Article Snippet:
Techniques: DNA Extraction, SYBR Green Assay