Journal: Frontiers in Immunology
Article Title: TRAF6 Regulates the Immunosuppressive Effects of Myeloid-Derived Suppressor Cells in Tumor-Bearing Host
Figure Lengend Snippet: TRAF6 knockdown impairs the immunosuppressive effects of MDSCs in vitro . Specific siRNA (siTRAF6) was used to knockdown the expression of TRAF6 in MDSCs, and the efficiency of siTRAF6 knockdown was validated by qRT-PCR (A) and Western blotting (B) . (C) Tumor-derived MDSCs were transfected with siTRAF6 and cocultured with CFSE-labeled CD4 + T cells, and proliferation was measured by flow cytometry after 72 h. (D) Statistical analyses of the percentage of proliferating CD4 + T cells co-cultured with MDSCs transfected with siTRAF6. After TRAF6 knockdown, the activity of Arg1 was measured by a QuantiChrom arginase assay kit (E) , and the concentration of NO was determined via a Griess reagent system kit (F) . *** p
Article Snippet: Isolation of MDSCs and CD4+ T Cells Murine Gr1+ CD11b+ MDSCs were isolated using a mouse MDSC kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.
Techniques: In Vitro, Expressing, Quantitative RT-PCR, Western Blot, Derivative Assay, Transfection, Labeling, Flow Cytometry, Cell Culture, Activity Assay, Arginase Assay, Concentration Assay