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    Miltenyi Biotec mdscs
    TRAF6 knockdown impairs the immunosuppressive effects of <t>MDSCs</t> in vitro . Specific siRNA (siTRAF6) was used to knockdown the expression of TRAF6 in MDSCs, and the efficiency of siTRAF6 knockdown was validated by qRT-PCR (A) and Western blotting (B) . (C) Tumor-derived MDSCs were transfected with siTRAF6 and cocultured with CFSE-labeled <t>CD4</t> + T cells, and proliferation was measured by flow cytometry after 72 h. (D) Statistical analyses of the percentage of proliferating CD4 + T cells co-cultured with MDSCs transfected with siTRAF6. After TRAF6 knockdown, the activity of Arg1 was measured by a QuantiChrom arginase assay kit (E) , and the concentration of NO was determined via a Griess reagent system kit (F) . *** p
    Mdscs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho Paxillin Thr 538 Blocking Peptide
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    Paxillin Thr 538 phospho specific Rabbit Polyclonal
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    TRAF6 knockdown impairs the immunosuppressive effects of MDSCs in vitro . Specific siRNA (siTRAF6) was used to knockdown the expression of TRAF6 in MDSCs, and the efficiency of siTRAF6 knockdown was validated by qRT-PCR (A) and Western blotting (B) . (C) Tumor-derived MDSCs were transfected with siTRAF6 and cocultured with CFSE-labeled CD4 + T cells, and proliferation was measured by flow cytometry after 72 h. (D) Statistical analyses of the percentage of proliferating CD4 + T cells co-cultured with MDSCs transfected with siTRAF6. After TRAF6 knockdown, the activity of Arg1 was measured by a QuantiChrom arginase assay kit (E) , and the concentration of NO was determined via a Griess reagent system kit (F) . *** p

    Journal: Frontiers in Immunology

    Article Title: TRAF6 Regulates the Immunosuppressive Effects of Myeloid-Derived Suppressor Cells in Tumor-Bearing Host

    doi: 10.3389/fimmu.2021.649020

    Figure Lengend Snippet: TRAF6 knockdown impairs the immunosuppressive effects of MDSCs in vitro . Specific siRNA (siTRAF6) was used to knockdown the expression of TRAF6 in MDSCs, and the efficiency of siTRAF6 knockdown was validated by qRT-PCR (A) and Western blotting (B) . (C) Tumor-derived MDSCs were transfected with siTRAF6 and cocultured with CFSE-labeled CD4 + T cells, and proliferation was measured by flow cytometry after 72 h. (D) Statistical analyses of the percentage of proliferating CD4 + T cells co-cultured with MDSCs transfected with siTRAF6. After TRAF6 knockdown, the activity of Arg1 was measured by a QuantiChrom arginase assay kit (E) , and the concentration of NO was determined via a Griess reagent system kit (F) . *** p

    Article Snippet: Isolation of MDSCs and CD4+ T Cells Murine Gr1+ CD11b+ MDSCs were isolated using a mouse MDSC kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: In Vitro, Expressing, Quantitative RT-PCR, Western Blot, Derivative Assay, Transfection, Labeling, Flow Cytometry, Cell Culture, Activity Assay, Arginase Assay, Concentration Assay

    TRAF6 expression was augmented in MDSCs from lung cancer patients. To examine the modulation of TRAF6 in MDSCs from lung cancer patients, the level of TRAF6 in MDSCs was measured in the lung cancer patient group (LC) and healthy control group (HC). (A) The proportions of MDSCs in the PBMCs of lung cancer patients and healthy persons were analyzed by flow cytometry. Representative dot plots of CD11b + CD33 + HLA-DR − MDSCs in the blood of patients with LC and healthy controls are shown. (B) The mean fluorescence intensity (MFI) of TRAF6 in MDSCs was determined by flow cytometry. (C) The MFI of arginase-1 in MDSCs was determined by flow cytometry. (D) The correlation between TRAF6 and arginase-1 in MDSCs was analyzed. *** p

    Journal: Frontiers in Immunology

    Article Title: TRAF6 Regulates the Immunosuppressive Effects of Myeloid-Derived Suppressor Cells in Tumor-Bearing Host

    doi: 10.3389/fimmu.2021.649020

    Figure Lengend Snippet: TRAF6 expression was augmented in MDSCs from lung cancer patients. To examine the modulation of TRAF6 in MDSCs from lung cancer patients, the level of TRAF6 in MDSCs was measured in the lung cancer patient group (LC) and healthy control group (HC). (A) The proportions of MDSCs in the PBMCs of lung cancer patients and healthy persons were analyzed by flow cytometry. Representative dot plots of CD11b + CD33 + HLA-DR − MDSCs in the blood of patients with LC and healthy controls are shown. (B) The mean fluorescence intensity (MFI) of TRAF6 in MDSCs was determined by flow cytometry. (C) The MFI of arginase-1 in MDSCs was determined by flow cytometry. (D) The correlation between TRAF6 and arginase-1 in MDSCs was analyzed. *** p

    Article Snippet: Isolation of MDSCs and CD4+ T Cells Murine Gr1+ CD11b+ MDSCs were isolated using a mouse MDSC kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Expressing, Flow Cytometry, Fluorescence

    TRAF6 is highly expressed in MDSCs derived from the tumor tissue of tumor-bearing mice. Approximately 1 × 10 6 LLC cells were s.c. injected in the backs of C57BL/6 mice for 28 d to establish a tumor-bearing (TB) mouse model. MDSCs were isolated by immunomagnetic beads from the spleens of TB mice, the tumor tissue of TB mice or the spleens of wild-type (WT) mice. (A) The purity of the isolated MDSCs was determined using flow cytometry via the detection of the CD11b + Gr1 + phenotype. The expression of TRAF6 in MDSCs derived from different sources was determined by qRT-PCR (B) or Western blotting (C) . The mRNA expression of TRAF6 in PMN-MDSCs and M-MDSCs derived from the spleen (D) or tumor tissue (E) . (F) CFSE-labeled CD4 + T cells were co-cultured with MDSCs derived from the spleen or tumor tissue in the presence of CD3 and CD28 stimulation. After 72 h, the proliferation of CD4 + T cells was tested via flow cytometry. (G) Statistical analyses of the percentage of proliferating CD4 + T cells co-cultured with MDSCs derived from the spleen or tumor tissue of TB mice. The mRNA expression levels of Arg1 (H) and iNOS (I) in MDSCs were measured by qRT-PCR. *** p

    Journal: Frontiers in Immunology

    Article Title: TRAF6 Regulates the Immunosuppressive Effects of Myeloid-Derived Suppressor Cells in Tumor-Bearing Host

    doi: 10.3389/fimmu.2021.649020

    Figure Lengend Snippet: TRAF6 is highly expressed in MDSCs derived from the tumor tissue of tumor-bearing mice. Approximately 1 × 10 6 LLC cells were s.c. injected in the backs of C57BL/6 mice for 28 d to establish a tumor-bearing (TB) mouse model. MDSCs were isolated by immunomagnetic beads from the spleens of TB mice, the tumor tissue of TB mice or the spleens of wild-type (WT) mice. (A) The purity of the isolated MDSCs was determined using flow cytometry via the detection of the CD11b + Gr1 + phenotype. The expression of TRAF6 in MDSCs derived from different sources was determined by qRT-PCR (B) or Western blotting (C) . The mRNA expression of TRAF6 in PMN-MDSCs and M-MDSCs derived from the spleen (D) or tumor tissue (E) . (F) CFSE-labeled CD4 + T cells were co-cultured with MDSCs derived from the spleen or tumor tissue in the presence of CD3 and CD28 stimulation. After 72 h, the proliferation of CD4 + T cells was tested via flow cytometry. (G) Statistical analyses of the percentage of proliferating CD4 + T cells co-cultured with MDSCs derived from the spleen or tumor tissue of TB mice. The mRNA expression levels of Arg1 (H) and iNOS (I) in MDSCs were measured by qRT-PCR. *** p

    Article Snippet: Isolation of MDSCs and CD4+ T Cells Murine Gr1+ CD11b+ MDSCs were isolated using a mouse MDSC kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Mouse Assay, Injection, Isolation, Flow Cytometry, Expressing, Quantitative RT-PCR, Western Blot, Labeling, Cell Culture

    TRAF6 knockdown attenuates the ability of MDSCs to accelerate tumor progression in tumor-bearing mice. To investigate the effects of TRAF6 on the suppressive activity of MDSCs in vivo , 2 groups of wild-type C57BL/6 mice were s.c. injected with 1 × 10 6 LLC cells and 1 × 10 6 MDSCs transfected with siTRAF6 (siTRAF6 group) or MDSCs transfected with siNC (control group). (A) Tumor growth was constantly monitored. The width “a” and length “b” were measured, and tumor volume was calculated. (B,C) On the 28th day after the inoculation of LLC cells, the mice were sacrificed, and the tumor image and weights were showed in both groups. (D) The proportion of CD4 + IFN-γ + Th1 cells in the tumor tissue of both groups was analyzed by FCM. (E) The proportion of CD8 + IFN-γ + CTLs in the tumor tissue of both groups was analyzed by FCM. * p

    Journal: Frontiers in Immunology

    Article Title: TRAF6 Regulates the Immunosuppressive Effects of Myeloid-Derived Suppressor Cells in Tumor-Bearing Host

    doi: 10.3389/fimmu.2021.649020

    Figure Lengend Snippet: TRAF6 knockdown attenuates the ability of MDSCs to accelerate tumor progression in tumor-bearing mice. To investigate the effects of TRAF6 on the suppressive activity of MDSCs in vivo , 2 groups of wild-type C57BL/6 mice were s.c. injected with 1 × 10 6 LLC cells and 1 × 10 6 MDSCs transfected with siTRAF6 (siTRAF6 group) or MDSCs transfected with siNC (control group). (A) Tumor growth was constantly monitored. The width “a” and length “b” were measured, and tumor volume was calculated. (B,C) On the 28th day after the inoculation of LLC cells, the mice were sacrificed, and the tumor image and weights were showed in both groups. (D) The proportion of CD4 + IFN-γ + Th1 cells in the tumor tissue of both groups was analyzed by FCM. (E) The proportion of CD8 + IFN-γ + CTLs in the tumor tissue of both groups was analyzed by FCM. * p

    Article Snippet: Isolation of MDSCs and CD4+ T Cells Murine Gr1+ CD11b+ MDSCs were isolated using a mouse MDSC kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Mouse Assay, Activity Assay, In Vivo, Injection, Transfection