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  • 99
    ATCC corynebacterium glutamicum 534 ncib 10025
    Corynebacterium Glutamicum 534 Ncib 10025, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/corynebacterium glutamicum 534 ncib 10025/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    corynebacterium glutamicum 534 ncib 10025 - by Bioz Stars, 2021-07
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    85
    ATCC bvdv strain nadl
    Fusion from without of <t>BVDV</t> in the presence or absence of DTT. MDBK cells were inoculated with BVDV strain <t>NADL</t> and briefly shifted to 37°C at the indicated pH in the presence or absence of 10 mM DTT; virus uptake via endocytosis was blocked by replacing buffer with DMEM containing bafilomycin A1. Higher concentrations of DTT could not be used due to high cell toxicity. The numbers of infectious centers were determined 12 to 16 h p.i. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.
    Bvdv Strain Nadl, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bvdv strain nadl - by Bioz Stars, 2021-07
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    Category Protein Products Peptides P69 522 534 M leprae Size 5 mg
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    N/A
    Rabbit polyclonal antibody to Tau phospho 534 Isotype Note IgG Host Note Rabbit Conjugation Note Unconjugated Reactivity Note Human Mouse Application Note WB
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    Fusion from without of BVDV in the presence or absence of DTT. MDBK cells were inoculated with BVDV strain NADL and briefly shifted to 37°C at the indicated pH in the presence or absence of 10 mM DTT; virus uptake via endocytosis was blocked by replacing buffer with DMEM containing bafilomycin A1. Higher concentrations of DTT could not be used due to high cell toxicity. The numbers of infectious centers were determined 12 to 16 h p.i. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: Fusion from without of BVDV in the presence or absence of DTT. MDBK cells were inoculated with BVDV strain NADL and briefly shifted to 37°C at the indicated pH in the presence or absence of 10 mM DTT; virus uptake via endocytosis was blocked by replacing buffer with DMEM containing bafilomycin A1. Higher concentrations of DTT could not be used due to high cell toxicity. The numbers of infectious centers were determined 12 to 16 h p.i. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques:

    pH stability of BVDV. A total of 2 × 10 6 PFU of BVDV strain NADL were incubated in citrate-phosphate buffers of a defined pH (pH 3.0 to 7.0) in the presence of 10 mM DTT for 15 min at 25°C and titrated on MDBK cells. The same experiment was performed in the absence of DTT, but only the infectivity after treatment at pH 3.0 and 7.0 is indicated. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: pH stability of BVDV. A total of 2 × 10 6 PFU of BVDV strain NADL were incubated in citrate-phosphate buffers of a defined pH (pH 3.0 to 7.0) in the presence of 10 mM DTT for 15 min at 25°C and titrated on MDBK cells. The same experiment was performed in the absence of DTT, but only the infectivity after treatment at pH 3.0 and 7.0 is indicated. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques: Incubation, Infection

    Effect of chlorpromazine and β-MCD on BVDV and BHV-1 infection. BVDV NADL (dark gray bars) or BHV-1 (light gray bars) was adsorbed to MDBK cells, and the effects of chlorpromazine and β-MCD on infection were investigated. Susceptibility to BVDV infection was decreased up to 1,000-fold, whereas BHV-1 infection was inhibited five-fold by β-MCD but not by chlorpromazine. The columns represent mean values of duplicate experiments; bars indicate maximum and minimum values.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: Effect of chlorpromazine and β-MCD on BVDV and BHV-1 infection. BVDV NADL (dark gray bars) or BHV-1 (light gray bars) was adsorbed to MDBK cells, and the effects of chlorpromazine and β-MCD on infection were investigated. Susceptibility to BVDV infection was decreased up to 1,000-fold, whereas BHV-1 infection was inhibited five-fold by β-MCD but not by chlorpromazine. The columns represent mean values of duplicate experiments; bars indicate maximum and minimum values.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques: Infection

    Expression of Dyn K44A reduces susceptibility to BVDV infection. (a) Immunoblot of MDBK Tet on dynamin-overexpressing cell lines. Crude cell lysates from equal numbers of cells grown in the presence or absence of 10 μg of doxycycline/ml were separated. After induction, a 99-kDa band of each HA-tagged protein is visible. (b) Inhibition of BVDV NADL/SinV infection by overexpression of dominant-negative Dyn K44A . Each indicated cell line was tested for its susceptibility to BVDV or SinV infection by inoculation with 2 × 10 5 PFU of BVDV strain NADL or SinV, respectively. MDBK cells overexpressing mutant dynamin after induction with doxycycline exhibited a 10-fold- reduced susceptibility compared to MDBK cells. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: Expression of Dyn K44A reduces susceptibility to BVDV infection. (a) Immunoblot of MDBK Tet on dynamin-overexpressing cell lines. Crude cell lysates from equal numbers of cells grown in the presence or absence of 10 μg of doxycycline/ml were separated. After induction, a 99-kDa band of each HA-tagged protein is visible. (b) Inhibition of BVDV NADL/SinV infection by overexpression of dominant-negative Dyn K44A . Each indicated cell line was tested for its susceptibility to BVDV or SinV infection by inoculation with 2 × 10 5 PFU of BVDV strain NADL or SinV, respectively. MDBK cells overexpressing mutant dynamin after induction with doxycycline exhibited a 10-fold- reduced susceptibility compared to MDBK cells. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques: Expressing, Infection, Inhibition, Over Expression, Dominant Negative Mutation, Mutagenesis

    Effect of different inhibitors of endosomal acidification on BVDV and BHV-1 infection. Directly after adsorption of BVDV NADL (dark gray bars) or BHV-1 (light gray bars) to MDBK cells, different inhibitors of endosomal acidification (bafilomycin A1, chloroquine, or ammonium chloride) were applied to determine the pH dependence of viral entry. Each inhibitor of endosomal acidification blocks BVDV infection in a concentration-dependent manner, whereas BHV-1 infection is not affected. The columns represent mean values of duplicate experiments; bars indicate maximum and minimum values.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: Effect of different inhibitors of endosomal acidification on BVDV and BHV-1 infection. Directly after adsorption of BVDV NADL (dark gray bars) or BHV-1 (light gray bars) to MDBK cells, different inhibitors of endosomal acidification (bafilomycin A1, chloroquine, or ammonium chloride) were applied to determine the pH dependence of viral entry. Each inhibitor of endosomal acidification blocks BVDV infection in a concentration-dependent manner, whereas BHV-1 infection is not affected. The columns represent mean values of duplicate experiments; bars indicate maximum and minimum values.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques: Infection, Adsorption, Concentration Assay

    Fusion from without of BVDV and SinV. MDBK cells were inoculated with BVDV strain NADL or SinV, respectively, for 1 h at 4°C. Medium was replaced by prewarmed buffers of the indicated pH, followed by incubation for 2 min at 37°C. Viral uptake via endocytosis was blocked by replacing buffer with DMEM containing bafilomycin A1. Since fusion from without is cell type specific, SinV was used as control. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: Fusion from without of BVDV and SinV. MDBK cells were inoculated with BVDV strain NADL or SinV, respectively, for 1 h at 4°C. Medium was replaced by prewarmed buffers of the indicated pH, followed by incubation for 2 min at 37°C. Viral uptake via endocytosis was blocked by replacing buffer with DMEM containing bafilomycin A1. Since fusion from without is cell type specific, SinV was used as control. The columns represent mean values of triplicate experiments; bars indicate maximum and minimum values.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques: Incubation

    Comparison of CxxC motifs from different viruses. A decapeptide of BVDV strain NADL and CSFV Alfort E2 containing the CxxC motif was aligned to the corresponding sequence in rubella virus E1 (GI:33415288) and Mo-MuLV gPr80 glycosylated envelope polyprotein (GI:18448745). The numbers denote the position of the decapeptide in the respective protein; the conserved CxxC motif is indicated above the sequence. Conserved cysteine residues are boxed.

    Journal: Journal of Virology

    Article Title: Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry

    doi: 10.1128/JVI.79.7.4191-4200.2005

    Figure Lengend Snippet: Comparison of CxxC motifs from different viruses. A decapeptide of BVDV strain NADL and CSFV Alfort E2 containing the CxxC motif was aligned to the corresponding sequence in rubella virus E1 (GI:33415288) and Mo-MuLV gPr80 glycosylated envelope polyprotein (GI:18448745). The numbers denote the position of the decapeptide in the respective protein; the conserved CxxC motif is indicated above the sequence. Conserved cysteine residues are boxed.

    Article Snippet: BVDV strain NADL (ATCC no. VR-534) and bovine herpesvirus 1 “M” (BHV-1; W. Eichhorn, Munich, Germany) were propagated on MDBK cells and stored at −70°C.

    Techniques: Sequencing