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  • 93
    Santa Cruz Biotechnology anti cd5
    Antigens expressed in Neu-medullocytes as B cells: IgG, <t>CD5,</t> IgM, and MHC class II. ( I ) Reconfirmation of the expression of IgG on X-NANA-stained cells using F(ab′) 2 fragment of anti-mouse IgG as detected by confocal microscopy. The thymus from a C57BL/6 mouse (male, 6 W) was used. ( A ) X-NANA-stained; ( B ) FITC-anti-mouse IgG; ( C and D ) merged ( A and B ). ( D and E ) enlarged area enclosed by a white square in ( C ) and its DIC (differential interference contrast) image, respectively. ( F ) DIC image including the areas from ( A – C ) (white square) and ( D , E ) (black square). Scale bars in ( A , D and F ) indicate 50 (for A – C ), 20 (for D and E ) and 100 µm (for F ), respectively. ( II ) Other antigens expressed on X-NANA stained cells. The thymus sections from C57BL/6 mice were used for ( A – C ) (male, 6 W). The thymus sections from AKR mice (haplotype k) were used for ( D – F ) (male, 8 W) and for ( G – I ) (female, 5 W). ( A , D and G ) X-NANA stained; ( B ) FITC-anti-CD5 stained; ( C ) merged ( A and B) ; ( E ) FITC-anti-MHC class II (anti I-A k ) stained; ( F ) merged ( D and E ); ( H ) R.R.-anti-IgM stained; ( I ) merged ( G and H ). The scale bars (μm) indicate 20 in A (for A – C ), 20 in D (for D – F ), and 50 in G (for G – I ).
    Anti Cd5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd5/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd5 - by Bioz Stars, 2022-12
    93/100 stars
      Buy from Supplier

    91
    Addgene inc pfa6a kanmx6 pgal1 gfp
    Antigens expressed in Neu-medullocytes as B cells: IgG, <t>CD5,</t> IgM, and MHC class II. ( I ) Reconfirmation of the expression of IgG on X-NANA-stained cells using F(ab′) 2 fragment of anti-mouse IgG as detected by confocal microscopy. The thymus from a C57BL/6 mouse (male, 6 W) was used. ( A ) X-NANA-stained; ( B ) FITC-anti-mouse IgG; ( C and D ) merged ( A and B ). ( D and E ) enlarged area enclosed by a white square in ( C ) and its DIC (differential interference contrast) image, respectively. ( F ) DIC image including the areas from ( A – C ) (white square) and ( D , E ) (black square). Scale bars in ( A , D and F ) indicate 50 (for A – C ), 20 (for D and E ) and 100 µm (for F ), respectively. ( II ) Other antigens expressed on X-NANA stained cells. The thymus sections from C57BL/6 mice were used for ( A – C ) (male, 6 W). The thymus sections from AKR mice (haplotype k) were used for ( D – F ) (male, 8 W) and for ( G – I ) (female, 5 W). ( A , D and G ) X-NANA stained; ( B ) FITC-anti-CD5 stained; ( C ) merged ( A and B) ; ( E ) FITC-anti-MHC class II (anti I-A k ) stained; ( F ) merged ( D and E ); ( H ) R.R.-anti-IgM stained; ( I ) merged ( G and H ). The scale bars (μm) indicate 20 in A (for A – C ), 20 in D (for D – F ), and 50 in G (for G – I ).
    Pfa6a Kanmx6 Pgal1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfa6a kanmx6 pgal1 gfp/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pfa6a kanmx6 pgal1 gfp - by Bioz Stars, 2022-12
    91/100 stars
      Buy from Supplier

    Image Search Results


    Antigens expressed in Neu-medullocytes as B cells: IgG, CD5, IgM, and MHC class II. ( I ) Reconfirmation of the expression of IgG on X-NANA-stained cells using F(ab′) 2 fragment of anti-mouse IgG as detected by confocal microscopy. The thymus from a C57BL/6 mouse (male, 6 W) was used. ( A ) X-NANA-stained; ( B ) FITC-anti-mouse IgG; ( C and D ) merged ( A and B ). ( D and E ) enlarged area enclosed by a white square in ( C ) and its DIC (differential interference contrast) image, respectively. ( F ) DIC image including the areas from ( A – C ) (white square) and ( D , E ) (black square). Scale bars in ( A , D and F ) indicate 50 (for A – C ), 20 (for D and E ) and 100 µm (for F ), respectively. ( II ) Other antigens expressed on X-NANA stained cells. The thymus sections from C57BL/6 mice were used for ( A – C ) (male, 6 W). The thymus sections from AKR mice (haplotype k) were used for ( D – F ) (male, 8 W) and for ( G – I ) (female, 5 W). ( A , D and G ) X-NANA stained; ( B ) FITC-anti-CD5 stained; ( C ) merged ( A and B) ; ( E ) FITC-anti-MHC class II (anti I-A k ) stained; ( F ) merged ( D and E ); ( H ) R.R.-anti-IgM stained; ( I ) merged ( G and H ). The scale bars (μm) indicate 20 in A (for A – C ), 20 in D (for D – F ), and 50 in G (for G – I ).

    Journal: Scientific Reports

    Article Title: Neu-medullocytes, sialidase-positive B cells in the thymus, express autoimmune regulator (AIRE)

    doi: 10.1038/s41598-018-37225-y

    Figure Lengend Snippet: Antigens expressed in Neu-medullocytes as B cells: IgG, CD5, IgM, and MHC class II. ( I ) Reconfirmation of the expression of IgG on X-NANA-stained cells using F(ab′) 2 fragment of anti-mouse IgG as detected by confocal microscopy. The thymus from a C57BL/6 mouse (male, 6 W) was used. ( A ) X-NANA-stained; ( B ) FITC-anti-mouse IgG; ( C and D ) merged ( A and B ). ( D and E ) enlarged area enclosed by a white square in ( C ) and its DIC (differential interference contrast) image, respectively. ( F ) DIC image including the areas from ( A – C ) (white square) and ( D , E ) (black square). Scale bars in ( A , D and F ) indicate 50 (for A – C ), 20 (for D and E ) and 100 µm (for F ), respectively. ( II ) Other antigens expressed on X-NANA stained cells. The thymus sections from C57BL/6 mice were used for ( A – C ) (male, 6 W). The thymus sections from AKR mice (haplotype k) were used for ( D – F ) (male, 8 W) and for ( G – I ) (female, 5 W). ( A , D and G ) X-NANA stained; ( B ) FITC-anti-CD5 stained; ( C ) merged ( A and B) ; ( E ) FITC-anti-MHC class II (anti I-A k ) stained; ( F ) merged ( D and E ); ( H ) R.R.-anti-IgM stained; ( I ) merged ( G and H ). The scale bars (μm) indicate 20 in A (for A – C ), 20 in D (for D – F ), and 50 in G (for G – I ).

    Article Snippet: The following antibodies were purchased: FITC-conjugated F(ab′)2 fragment of donkey anti-mouse IgG (H + L) (Jackson ImmunoResearch, West Grove, PA), anti-CD5 (Q-20, goat polyclonal IgG for mouse CD5 at the N-terminus, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), anti-NEU1 middle region antibody (rabbit polyclonal antibody, Aviva Systems Biology (San Diego, CA)), Anti-AIRE (rabbit polyclonal IgG against AIRE (KLH-conjugated synthetic peptide derived from human AIRE, purified by protein A (cross-reactive species: human, mouse, rat), from Bioss (Boston, MA)).

    Techniques: Expressing, Staining, Confocal Microscopy, Mouse Assay

    CD5 expression confers thymocyte survival advantage. ( A , together with Annexin V and 7AAD to assess cell death. The populations were grouped based on top and bottom 20% of CD5 expression within each population. Representative flow plots are shown. ( B ) The frequencies of Annexin V + cells within the top and bottom 20% of CD5 expressers are shown ( n = 4 from 1 experiment is shown and n = 3 from a second independent experiment). ( C , together with Annexin V and 7AAD to assess cell death. Representative flow plots are shown for the double-positive CD69 hi population. ( D ) The frequencies of Annexin V + cells within the top and bottom 20% of CD5 expressers of the double-positive CD69 hi population are shown ( n = 3 from 1 independent experiment). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

    doi: 10.1073/pnas.1922525117

    Figure Lengend Snippet: CD5 expression confers thymocyte survival advantage. ( A , together with Annexin V and 7AAD to assess cell death. The populations were grouped based on top and bottom 20% of CD5 expression within each population. Representative flow plots are shown. ( B ) The frequencies of Annexin V + cells within the top and bottom 20% of CD5 expressers are shown ( n = 4 from 1 experiment is shown and n = 3 from a second independent experiment). ( C , together with Annexin V and 7AAD to assess cell death. Representative flow plots are shown for the double-positive CD69 hi population. ( D ) The frequencies of Annexin V + cells within the top and bottom 20% of CD5 expressers of the double-positive CD69 hi population are shown ( n = 3 from 1 independent experiment). * P

    Article Snippet: Membranes were blocked with 5% skim milk at room temperature for 1 h. Membranes were incubated with primary antibodies of anti-CD5 (SC6986, SantaCruz Biotechnology), anti-IκBα (9242, Cell Signaling Technology), antiphosphor-SHP-1(Y564) (8849, Cell Signaling Technology), antiactin (A5441, Sigma Aldrich), anti-PLCγ1 (SC81, SantaCruz Biotechnology), anti-p65 NF-κB (SC372, SantaCruz Biotechnology), or anti-SP1 (SC59, SantaCruz Biotechnology), for 4 h followed by HRP-conjugated anti-mouse IgG, anti-rabbit IgG or anti-goat IgG.

    Techniques: Expressing

    The CD5 hi and CD5 lo fractions of peripheral T cells show differential pool of cytoplasmic NF-κB. ( A ) gMFI of NF-κB in peripheral CD4 and CD8 T cells isolated from lymph nodes ( n = 8 from 3 independent experiments). ( B ) Purified WT T cells were stained and analyzed by imaging flow cytometry. Screenshot of dashboard from IDEAS analysis from data collected on Amnis ImageStream. ( C ) Mean NF-κB expressed as a ratio of CD5 hi to CD5 lo within CD4 and CD8 peripheral resting T cells ( n = 5 from 1 independent experiment). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

    doi: 10.1073/pnas.1922525117

    Figure Lengend Snippet: The CD5 hi and CD5 lo fractions of peripheral T cells show differential pool of cytoplasmic NF-κB. ( A ) gMFI of NF-κB in peripheral CD4 and CD8 T cells isolated from lymph nodes ( n = 8 from 3 independent experiments). ( B ) Purified WT T cells were stained and analyzed by imaging flow cytometry. Screenshot of dashboard from IDEAS analysis from data collected on Amnis ImageStream. ( C ) Mean NF-κB expressed as a ratio of CD5 hi to CD5 lo within CD4 and CD8 peripheral resting T cells ( n = 5 from 1 independent experiment). * P

    Article Snippet: Membranes were blocked with 5% skim milk at room temperature for 1 h. Membranes were incubated with primary antibodies of anti-CD5 (SC6986, SantaCruz Biotechnology), anti-IκBα (9242, Cell Signaling Technology), antiphosphor-SHP-1(Y564) (8849, Cell Signaling Technology), antiactin (A5441, Sigma Aldrich), anti-PLCγ1 (SC81, SantaCruz Biotechnology), anti-p65 NF-κB (SC372, SantaCruz Biotechnology), or anti-SP1 (SC59, SantaCruz Biotechnology), for 4 h followed by HRP-conjugated anti-mouse IgG, anti-rabbit IgG or anti-goat IgG.

    Techniques: Isolation, Purification, Staining, Imaging, Flow Cytometry

    Concomitant up-regulation of IκBα with developmental and peripheral modulation of CD5 levels. ( A ). DP = CD4 + CD8 + and SP = single positive for either CD4 or CD8 expression as designated. ( B ) The CD5 and IκBα gMFI on thymocyte subsets as identified in A ( n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). ( C ) Representative peripheral expression levels of CD5 and IκBα in T cells gated by CD4 + , TCR-β + , and CD25 expression, to enrich for regulatory T cells in pooled lymph node (the CD25 hi population). ( D ) The CD5 and IκBα gMFI on CD25 hi and CD25 lo T cells ( n = 7 from 3 independent experiments; statistical significance was calculated using paired t tests). ( E ) 5C.C7 TCR-tg CD4 + T cells were adoptively transferred to congenically distinct B10.A mice and challenged with MCC peptide and LPS challenge in B10.A hosts. Expression of CD5 was analyzed at varying time points after challenge on adoptively transferred TCR-tg CD4 + T cells ( n = 2 to 3 from 1 independent experiment; statistical significance was calculated using Tukey’s multiple comparisons). ( F ) Representative flow plots gated on live, TCR-β + and CD4 + or CD8 + ). ( G ) CD5 and IκBα gMFI on CD4 or CD8 cells gated as indicated in F from lymph node or spleen ( n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

    doi: 10.1073/pnas.1922525117

    Figure Lengend Snippet: Concomitant up-regulation of IκBα with developmental and peripheral modulation of CD5 levels. ( A ). DP = CD4 + CD8 + and SP = single positive for either CD4 or CD8 expression as designated. ( B ) The CD5 and IκBα gMFI on thymocyte subsets as identified in A ( n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). ( C ) Representative peripheral expression levels of CD5 and IκBα in T cells gated by CD4 + , TCR-β + , and CD25 expression, to enrich for regulatory T cells in pooled lymph node (the CD25 hi population). ( D ) The CD5 and IκBα gMFI on CD25 hi and CD25 lo T cells ( n = 7 from 3 independent experiments; statistical significance was calculated using paired t tests). ( E ) 5C.C7 TCR-tg CD4 + T cells were adoptively transferred to congenically distinct B10.A mice and challenged with MCC peptide and LPS challenge in B10.A hosts. Expression of CD5 was analyzed at varying time points after challenge on adoptively transferred TCR-tg CD4 + T cells ( n = 2 to 3 from 1 independent experiment; statistical significance was calculated using Tukey’s multiple comparisons). ( F ) Representative flow plots gated on live, TCR-β + and CD4 + or CD8 + ). ( G ) CD5 and IκBα gMFI on CD4 or CD8 cells gated as indicated in F from lymph node or spleen ( n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). * P

    Article Snippet: Membranes were blocked with 5% skim milk at room temperature for 1 h. Membranes were incubated with primary antibodies of anti-CD5 (SC6986, SantaCruz Biotechnology), anti-IκBα (9242, Cell Signaling Technology), antiphosphor-SHP-1(Y564) (8849, Cell Signaling Technology), antiactin (A5441, Sigma Aldrich), anti-PLCγ1 (SC81, SantaCruz Biotechnology), anti-p65 NF-κB (SC372, SantaCruz Biotechnology), or anti-SP1 (SC59, SantaCruz Biotechnology), for 4 h followed by HRP-conjugated anti-mouse IgG, anti-rabbit IgG or anti-goat IgG.

    Techniques: Expressing, Mouse Assay

    Components of NF-κB signaling are selectively altered in CD5 hi compared to CD5 lo peripheral T cells. The surface levels of CD5 and intracellular levels of Zap70 ( A ), PLCγ ( B ), LAT ( C ), IκBα ( D ), or NF-κB ( E ), are shown for either CD4 ( Left ) or CD8 ( Right ) T cells. The gMFI of CD5 ( F ), IκBα ( G ), or NFκB ( H ) expressed by CD4 or CD8 T cells from lymph node or spleen cells, gated on live, TCR-β + cells ( n = 11 from 3 independent experiments). Statistical significance was calculated using a paired t test. *** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

    doi: 10.1073/pnas.1922525117

    Figure Lengend Snippet: Components of NF-κB signaling are selectively altered in CD5 hi compared to CD5 lo peripheral T cells. The surface levels of CD5 and intracellular levels of Zap70 ( A ), PLCγ ( B ), LAT ( C ), IκBα ( D ), or NF-κB ( E ), are shown for either CD4 ( Left ) or CD8 ( Right ) T cells. The gMFI of CD5 ( F ), IκBα ( G ), or NFκB ( H ) expressed by CD4 or CD8 T cells from lymph node or spleen cells, gated on live, TCR-β + cells ( n = 11 from 3 independent experiments). Statistical significance was calculated using a paired t test. *** P

    Article Snippet: Membranes were blocked with 5% skim milk at room temperature for 1 h. Membranes were incubated with primary antibodies of anti-CD5 (SC6986, SantaCruz Biotechnology), anti-IκBα (9242, Cell Signaling Technology), antiphosphor-SHP-1(Y564) (8849, Cell Signaling Technology), antiactin (A5441, Sigma Aldrich), anti-PLCγ1 (SC81, SantaCruz Biotechnology), anti-p65 NF-κB (SC372, SantaCruz Biotechnology), or anti-SP1 (SC59, SantaCruz Biotechnology), for 4 h followed by HRP-conjugated anti-mouse IgG, anti-rabbit IgG or anti-goat IgG.

    Techniques:

    CD5-IκBα correlation is independent of SHP-1 and has a saturable level of IκBα up-regulation. ( A ) Western blot analysis of thymocytes from WT and SHP-1–KO animals ( n = 1 from 1 independent experiment). ( B ) Flow cytometry analysis of WT and SHP-1–KO splenocytes ( n = 1 from 1 independent experiment). ( C ) CD5 and IκBα gMFI in WT and SHP-1–KO splenocytes ( n = 1 from 1 independent experiment). ( D ) CD5 and IκBα gMFI of peripheral T cells after 24 h ex vivo culture in the presence or absence of varying concentrations CX4945 ( n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). ( E ) WT and CD5 transgenic (CD5-Tg) thymocytes were harvested and analyzed for expression of CD5 and IκBα. Representative flow plots are shown. ( F ) CD5 and IκBα gMFI on peripheral CD4 and CD8 T cells from WT and CD5-Tg animals ( n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). ( G . Frequency of population in each bin is displayed for CD4 and CD8 peripheral T cells ( n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). ( H ) Lymphocytes from SMARTA CD4 + TCR-Tg mice were isolated and stimulated ex vivo with TCR-αβ KO antigen presenting cells and cognate peptide for the indicated time points. Cells were harvested and analyzed by flow cytometry. CD5 and IκBα gMFI of SMARTA CD4 + TCR-Tg T cells ( n = 1 from 1 independent experiment). ( I ) BW-5147 cells were transduced with empty or CD5 retroviral vector. Cells were rested for 5 d after transduction and then analyzed by flow cytometry. Representative flow plots are shown. ( J ) CD5 and IκBα gMFI on retrovirally transduced BW-5147 cells ( n = 4 from 3 independent experiments; statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test). ** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

    doi: 10.1073/pnas.1922525117

    Figure Lengend Snippet: CD5-IκBα correlation is independent of SHP-1 and has a saturable level of IκBα up-regulation. ( A ) Western blot analysis of thymocytes from WT and SHP-1–KO animals ( n = 1 from 1 independent experiment). ( B ) Flow cytometry analysis of WT and SHP-1–KO splenocytes ( n = 1 from 1 independent experiment). ( C ) CD5 and IκBα gMFI in WT and SHP-1–KO splenocytes ( n = 1 from 1 independent experiment). ( D ) CD5 and IκBα gMFI of peripheral T cells after 24 h ex vivo culture in the presence or absence of varying concentrations CX4945 ( n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). ( E ) WT and CD5 transgenic (CD5-Tg) thymocytes were harvested and analyzed for expression of CD5 and IκBα. Representative flow plots are shown. ( F ) CD5 and IκBα gMFI on peripheral CD4 and CD8 T cells from WT and CD5-Tg animals ( n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). ( G . Frequency of population in each bin is displayed for CD4 and CD8 peripheral T cells ( n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). ( H ) Lymphocytes from SMARTA CD4 + TCR-Tg mice were isolated and stimulated ex vivo with TCR-αβ KO antigen presenting cells and cognate peptide for the indicated time points. Cells were harvested and analyzed by flow cytometry. CD5 and IκBα gMFI of SMARTA CD4 + TCR-Tg T cells ( n = 1 from 1 independent experiment). ( I ) BW-5147 cells were transduced with empty or CD5 retroviral vector. Cells were rested for 5 d after transduction and then analyzed by flow cytometry. Representative flow plots are shown. ( J ) CD5 and IκBα gMFI on retrovirally transduced BW-5147 cells ( n = 4 from 3 independent experiments; statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test). ** P

    Article Snippet: Membranes were blocked with 5% skim milk at room temperature for 1 h. Membranes were incubated with primary antibodies of anti-CD5 (SC6986, SantaCruz Biotechnology), anti-IκBα (9242, Cell Signaling Technology), antiphosphor-SHP-1(Y564) (8849, Cell Signaling Technology), antiactin (A5441, Sigma Aldrich), anti-PLCγ1 (SC81, SantaCruz Biotechnology), anti-p65 NF-κB (SC372, SantaCruz Biotechnology), or anti-SP1 (SC59, SantaCruz Biotechnology), for 4 h followed by HRP-conjugated anti-mouse IgG, anti-rabbit IgG or anti-goat IgG.

    Techniques: Western Blot, Flow Cytometry, Ex Vivo, Transgenic Assay, Expressing, Mouse Assay, Isolation, Transduction, Plasmid Preparation