Journal: Journal of Neuroinflammation
Article Title: Activation of the nuclear receptor PPARδ is neuroprotective in a transgenic mouse model of Alzheimer’s disease through inhibition of inflammation
Figure Lengend Snippet: GW0742 treatment decreased the expression levels of proinflammatory mediators in the brains of 5XFAD mice. The expression levels of C3 (A) , C1qa (B) , IL-6 (C) ,TNFα (D) , CCL2 (E) , INFγ (F) , CXCR2 (G) and IL-1β (H) in the brain of the 5XFAD mice were analyzed by qPCR. Results are presented as mean ± SEM. VEH = vehicle- and GW0742 = GW0742-treated mice. ** P < 0.01 *** P < 0.001 as analyzed by 1-way ANOVA followed by Tukey’s post hoc test. n = 10 in WT vehicle-treated group, n = 9 in TG vehicle- and n = 9 in TG GW0742-treated group.
Article Snippet: The sections were incubated with antibodies to glial fibrillary acidic protein (GFAP; 1:500 dilution, Dako, Carpinteria, CA, USA), ionized calcium binding adaptor molecule 1 (Iba-1; 1:200 dilution, Wako Chemicals, Richmond, VA, USA), NeuN (Aves Labs Inc, Tigard, Oregon, USA), complement component 3 (C3) and C1qa (1:1,000 dilution, both from Novus Biologicals, Littleton, CO, USA,), and 6E10 (BioLegend, Dedham, MA, USA) followed by incubation with appropriate Alexafluor 488 or 546 conjugated secondary antibodies (Molecular Probes/Life Technologies, Grand Island, NY, USA).