Journal: Journal of Neuroinflammation

Article Title: Activation of the nuclear receptor PPARδ is neuroprotective in a transgenic mouse model of Alzheimer’s disease through inhibition of inflammation

doi: 10.1186/s12974-014-0229-9

Figure Lengend Snippet: GW0742 treatment decreased the expression levels of proinflammatory mediators in the brains of 5XFAD mice. The expression levels of C3 (A) , C1qa (B) , IL-6 (C) ,TNFα (D) , CCL2 (E) , INFγ (F) , CXCR2 (G) and IL-1β (H) in the brain of the 5XFAD mice were analyzed by qPCR. Results are presented as mean ± SEM. VEH = vehicle- and GW0742 = GW0742-treated mice. ** P < 0.01 *** P < 0.001 as analyzed by 1-way ANOVA followed by Tukey’s post hoc test. n = 10 in WT vehicle-treated group, n = 9 in TG vehicle- and n = 9 in TG GW0742-treated group.

Article Snippet: The sections were incubated with antibodies to glial fibrillary acidic protein (GFAP; 1:500 dilution, Dako, Carpinteria, CA, USA), ionized calcium binding adaptor molecule 1 (Iba-1; 1:200 dilution, Wako Chemicals, Richmond, VA, USA), NeuN (Aves Labs Inc, Tigard, Oregon, USA), complement component 3 (C3) and C1qa (1:1,000 dilution, both from Novus Biologicals, Littleton, CO, USA,), and 6E10 (BioLegend, Dedham, MA, USA) followed by incubation with appropriate Alexafluor 488 or 546 conjugated secondary antibodies (Molecular Probes/Life Technologies, Grand Island, NY, USA).

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Activation of the nuclear receptor PPARδ is neuroprotective in a transgenic mouse model of Alzheimer’s disease through inhibition of inflammation

doi: 10.1186/s12974-014-0229-9

Figure Lengend Snippet: The expression levels of C1qa and C3 were reduced in GW0742-treated mouse brain as analyzed by immunohistochemistry. Immunohistochemistry against C1qa and C3 revealed a staining pattern associated predominantly with glial fibrillary acidic protein (GFAP) immunoreactivity. Figures (A) and (B) show typical example images of C1qa and GFAP immunoreactivities in vehicle-treated TG mice, respectively and figure (C) is the overlay of C1qa and GFAP. Figures (D-F) show example images of corresponding immunoreactivities in GW0742-treated TG mice. Figures (G-H) show C3 and GFAP immunoreactivities in vehicle-treated TG mice and figure (I) the overlay of C3 and GFAP. Figure (J-L) depict example images of corresponding immunoreactivities in GW0742-treated TG mice. The high magnification insets show typical C1qa and C3 staining pattern resembling astrocytes. Scale bar = 200 μm.

Article Snippet: The sections were incubated with antibodies to glial fibrillary acidic protein (GFAP; 1:500 dilution, Dako, Carpinteria, CA, USA), ionized calcium binding adaptor molecule 1 (Iba-1; 1:200 dilution, Wako Chemicals, Richmond, VA, USA), NeuN (Aves Labs Inc, Tigard, Oregon, USA), complement component 3 (C3) and C1qa (1:1,000 dilution, both from Novus Biologicals, Littleton, CO, USA,), and 6E10 (BioLegend, Dedham, MA, USA) followed by incubation with appropriate Alexafluor 488 or 546 conjugated secondary antibodies (Molecular Probes/Life Technologies, Grand Island, NY, USA).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Strains used in this study.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques:

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Sequence similarity of excised DNA fragments.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing