Article Title: A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types
Figure Lengend Snippet: ( A ) AAV–TREtight-myrmCherry expression in 56L. mCherry expression was restricted to mCitrine positive cells (mCitrine+ cells/ mCherry+ cells: 152/152). ( A2 ) higher magnification of injection site. ( B ) Co-infection of AAV–TRE3G–Cre and AAV–CAG–Flex-myrmCherry HAnsulator sequence from. There was strong non-specific mCherry expression near injection site ( B2 ). ( C ) TRE3G–Split Cre had specific expression of reporter without apparent leak. ( D ) TRE3G–Flpe had non-specific expression in a few cells ( D2 , arrows) ( E ) TRE3G split Cre had non-specific expression from Ai14 reporter allele in P113 subiculum. ( F ) TRE3G-nanobody Split Cre had specific expression (mCitrine+ cells/mCherry + cells: 64/64). ( F2 ) higher magnification of the injection site. ( G ) Specific expression of AAV Cre repoter by TRE3G-nanobody split Cre in 56L. DOI: http://dx.doi.org/10.7554/eLife.13503.017
Article Snippet: For nanobody-split Cre construction, GBP1 and GBP6 (Addgene #50791 and #50796, ) were fused with NCre and CCre from split Cre (gifts from ).
Techniques: Expressing, Injection, Infection, Sequencing