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  • 93
    Thermo Fisher iga human uncoated elisa kit
    Microbiota depletion impairs antibacterial defense in the lung by decreasing pulmonary <t>IgA</t> production. ( A – D ) Total levels of ( A ) IgM ( n = 8 for Conv; n = 5 for ABx mice), ( B ) IgG ( n = 5 for Conv; n = 4 for ABx mice), and ( C ) IgA ( n = 14 for Conv; n = 14 for ABx mice) in the BALF or ( D ) in plasma ( n = 9 for Conv; n = 7 for ABx mice) were measured. ( E ) Lung IgA + plasma cells were identified by FACS as IgA hi ( E , left) and B220 lo CD138 hi cells ( E , right). ( F ) Total lung IgA + cells and ( G ) IgA + plasma cells were quantified by FACS ( n = 10 for Conv and ABx mice). ( H ) Levels of P . aeruginosa –binding IgA were quantified by coating P . aeruginosa to <t>ELISA</t> plates and measuring bound IgA ( n = 7 for Conv and ABx mice). ( I , J ) WT and IgA –/– mice were pretreated or not with antibiotics, and afterwards infected with 1 × 10 5 CFU per mouse P . aeruginosa . Bacterial loads in the ( I ) lungs and ( J ) blood were measured at 24 hours after infection ( n = 24 for Conv WT; n = 28 for Conv IgA –/– ; n = 9 for WT ABx; n = 12 for IgA –/– ABx mice). Mann-Whitney U tests were applied to the data set. Values are mean ± SD. * P
    Iga Human Uncoated Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga human uncoated elisa kit/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga human uncoated elisa kit - by Bioz Stars, 2022-12
    93/100 stars
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    94
    Norgen Biotek plasma serum circulating dna purification mini kit
    Microbiota depletion impairs antibacterial defense in the lung by decreasing pulmonary <t>IgA</t> production. ( A – D ) Total levels of ( A ) IgM ( n = 8 for Conv; n = 5 for ABx mice), ( B ) IgG ( n = 5 for Conv; n = 4 for ABx mice), and ( C ) IgA ( n = 14 for Conv; n = 14 for ABx mice) in the BALF or ( D ) in plasma ( n = 9 for Conv; n = 7 for ABx mice) were measured. ( E ) Lung IgA + plasma cells were identified by FACS as IgA hi ( E , left) and B220 lo CD138 hi cells ( E , right). ( F ) Total lung IgA + cells and ( G ) IgA + plasma cells were quantified by FACS ( n = 10 for Conv and ABx mice). ( H ) Levels of P . aeruginosa –binding IgA were quantified by coating P . aeruginosa to <t>ELISA</t> plates and measuring bound IgA ( n = 7 for Conv and ABx mice). ( I , J ) WT and IgA –/– mice were pretreated or not with antibiotics, and afterwards infected with 1 × 10 5 CFU per mouse P . aeruginosa . Bacterial loads in the ( I ) lungs and ( J ) blood were measured at 24 hours after infection ( n = 24 for Conv WT; n = 28 for Conv IgA –/– ; n = 9 for WT ABx; n = 12 for IgA –/– ABx mice). Mann-Whitney U tests were applied to the data set. Values are mean ± SD. * P
    Plasma Serum Circulating Dna Purification Mini Kit, supplied by Norgen Biotek, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma serum circulating dna purification mini kit/product/Norgen Biotek
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasma serum circulating dna purification mini kit - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    90
    Novus Biologicals xpa
    Microbiota depletion impairs antibacterial defense in the lung by decreasing pulmonary <t>IgA</t> production. ( A – D ) Total levels of ( A ) IgM ( n = 8 for Conv; n = 5 for ABx mice), ( B ) IgG ( n = 5 for Conv; n = 4 for ABx mice), and ( C ) IgA ( n = 14 for Conv; n = 14 for ABx mice) in the BALF or ( D ) in plasma ( n = 9 for Conv; n = 7 for ABx mice) were measured. ( E ) Lung IgA + plasma cells were identified by FACS as IgA hi ( E , left) and B220 lo CD138 hi cells ( E , right). ( F ) Total lung IgA + cells and ( G ) IgA + plasma cells were quantified by FACS ( n = 10 for Conv and ABx mice). ( H ) Levels of P . aeruginosa –binding IgA were quantified by coating P . aeruginosa to <t>ELISA</t> plates and measuring bound IgA ( n = 7 for Conv and ABx mice). ( I , J ) WT and IgA –/– mice were pretreated or not with antibiotics, and afterwards infected with 1 × 10 5 CFU per mouse P . aeruginosa . Bacterial loads in the ( I ) lungs and ( J ) blood were measured at 24 hours after infection ( n = 24 for Conv WT; n = 28 for Conv IgA –/– ; n = 9 for WT ABx; n = 12 for IgA –/– ABx mice). Mann-Whitney U tests were applied to the data set. Values are mean ± SD. * P
    Xpa, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xpa/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xpa - by Bioz Stars, 2022-12
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    Image Search Results


    Microbiota depletion impairs antibacterial defense in the lung by decreasing pulmonary IgA production. ( A – D ) Total levels of ( A ) IgM ( n = 8 for Conv; n = 5 for ABx mice), ( B ) IgG ( n = 5 for Conv; n = 4 for ABx mice), and ( C ) IgA ( n = 14 for Conv; n = 14 for ABx mice) in the BALF or ( D ) in plasma ( n = 9 for Conv; n = 7 for ABx mice) were measured. ( E ) Lung IgA + plasma cells were identified by FACS as IgA hi ( E , left) and B220 lo CD138 hi cells ( E , right). ( F ) Total lung IgA + cells and ( G ) IgA + plasma cells were quantified by FACS ( n = 10 for Conv and ABx mice). ( H ) Levels of P . aeruginosa –binding IgA were quantified by coating P . aeruginosa to ELISA plates and measuring bound IgA ( n = 7 for Conv and ABx mice). ( I , J ) WT and IgA –/– mice were pretreated or not with antibiotics, and afterwards infected with 1 × 10 5 CFU per mouse P . aeruginosa . Bacterial loads in the ( I ) lungs and ( J ) blood were measured at 24 hours after infection ( n = 24 for Conv WT; n = 28 for Conv IgA –/– ; n = 9 for WT ABx; n = 12 for IgA –/– ABx mice). Mann-Whitney U tests were applied to the data set. Values are mean ± SD. * P

    Journal: The Journal of Clinical Investigation

    Article Title: Antibiotic treatment–induced secondary IgA deficiency enhances susceptibility to Pseudomonas aeruginosa pneumonia

    doi: 10.1172/JCI97065

    Figure Lengend Snippet: Microbiota depletion impairs antibacterial defense in the lung by decreasing pulmonary IgA production. ( A – D ) Total levels of ( A ) IgM ( n = 8 for Conv; n = 5 for ABx mice), ( B ) IgG ( n = 5 for Conv; n = 4 for ABx mice), and ( C ) IgA ( n = 14 for Conv; n = 14 for ABx mice) in the BALF or ( D ) in plasma ( n = 9 for Conv; n = 7 for ABx mice) were measured. ( E ) Lung IgA + plasma cells were identified by FACS as IgA hi ( E , left) and B220 lo CD138 hi cells ( E , right). ( F ) Total lung IgA + cells and ( G ) IgA + plasma cells were quantified by FACS ( n = 10 for Conv and ABx mice). ( H ) Levels of P . aeruginosa –binding IgA were quantified by coating P . aeruginosa to ELISA plates and measuring bound IgA ( n = 7 for Conv and ABx mice). ( I , J ) WT and IgA –/– mice were pretreated or not with antibiotics, and afterwards infected with 1 × 10 5 CFU per mouse P . aeruginosa . Bacterial loads in the ( I ) lungs and ( J ) blood were measured at 24 hours after infection ( n = 24 for Conv WT; n = 28 for Conv IgA –/– ; n = 9 for WT ABx; n = 12 for IgA –/– ABx mice). Mann-Whitney U tests were applied to the data set. Values are mean ± SD. * P

    Article Snippet: Levels of prostaglandin E2 (PGE2) and the following cytokines and immunoglobulins were measured by ELISAs according to the manufacturers’ recommendations: mPGE2 (R & D Systems, catalog KGE004B), mIL-1α (eBioscience, catalog 50-112-5107), mIL-1β (eBioscience, catalog 88-7013-88), mTNF-α (eBioscience, catalog 88-7324-88), mIL-6 (eBioscience, catalog 88-7064-88), mCXCL1 (R & D Systems, catalog DY453-05), mCXCL2 (R & D Systems, catalog DY452-05), CCL2 (eBioscience, catalog 88-7391-22), mBAFF (R & D Systems, catalog MBLYS0), mAPRIL (LSBio, catalog LS-F7691), mIgM (eBioscience, catalog 88-50470-22), mIgG (eBioscience, catalog 88-50400), mIgA (eBioscience, catalog 88-50450-77, or direct ELISA with anti–mouse IgA/HRP cross-adsorbed from Thermo Fisher Scientific, catalog PA1-74397), hIgA (Thermo Fisher Scientific, catalog 88-50600-77), hIgM (eBioscience, catalog 88-50620-22), and hIgG (Thermo Fisher Scientific, catalog BMS2091TEN).

    Techniques: Mouse Assay, FACS, Binding Assay, Enzyme-linked Immunosorbent Assay, Infection, MANN-WHITNEY