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    Covaris m220 ultrasonicator
    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using <t>Covaris</t> ® <t>M220</t> Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].
    M220 Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m220 ultrasonicator/product/Covaris
    Average 99 stars, based on 249 article reviews
    Price from $9.99 to $1999.99
    m220 ultrasonicator - by Bioz Stars, 2020-05
    99/100 stars
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    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Journal: Genes

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples

    doi: 10.3390/genes9010049

    Figure Lengend Snippet: Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Article Snippet: Nuclear DNA Sensitivity and Size Selection Study The single source male control DNA NA24129 (Coriell Institute for Medical Research, Camden, NJ, USA) was mechanically fragmented using the Covaris® M220 Focused-ultrasonicatorTM (Covaris, Woburn, MA, USA) to an average size of 175 bp with a range from 25 bp to 250 bp.

    Techniques: Next-Generation Sequencing, Sequencing, Amplification