5-methyl-dctp locus-specific primers Search Results


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  • 99
    Thermo Fisher phusion hot start dna polymerase
    Phusion Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 674 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TriLink 5 methyl dctp
    BAC clone sequencing based on the MspJI-based DNA fragmentation approach. ( a ) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM <t>5-methyl-dCTP</t> were included. ( b ) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.
    5 Methyl Dctp, supplied by TriLink, used in various techniques. Bioz Stars score: 91/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche expand long range dntpack
    BAC clone sequencing based on the MspJI-based DNA fragmentation approach. ( a ) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM <t>5-methyl-dCTP</t> were included. ( b ) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.
    Expand Long Range Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum pfx dna polymerase
    BAC clone sequencing based on the MspJI-based DNA fragmentation approach. ( a ) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM <t>5-methyl-dCTP</t> were included. ( b ) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.
    Platinum Pfx Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    platinum pfx dna polymerase - by Bioz Stars, 2020-08
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    Thermo Fisher platinum taq polymerase
    BAC clone sequencing based on the MspJI-based DNA fragmentation approach. ( a ) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM <t>5-methyl-dCTP</t> were included. ( b ) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.
    Platinum Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    BAC clone sequencing based on the MspJI-based DNA fragmentation approach. ( a ) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM 5-methyl-dCTP were included. ( b ) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.

    Journal: BMC Biotechnology

    Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

    doi: 10.1186/s12896-015-0139-7

    Figure Lengend Snippet: BAC clone sequencing based on the MspJI-based DNA fragmentation approach. ( a ) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM 5-methyl-dCTP were included. ( b ) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.

    Article Snippet: 5-methyl-dCTP (TriLink, CA, USA) was added to the PCR mixture at final concentrations of from 0 to 60 μM.

    Techniques: BAC Assay, Sequencing, Amplification, Clone Assay, Software

    Sequencing of the fungal endophyte genome with the MspJI-based DNA fragmentation method. Two independent experiments (rep1 and rep2) were performed using the single endophyte strain. ( a ) WGA of the endophyte genome with the Φ29 enzyme in the presence of 5-methyl-dCTP (X μM). ( b ) MspJI digestion of the endophyte genome-derived amplicons. ( c ) Flowchart of the MspJI- and physical shearing-based library prep procedures from WGA products.

    Journal: BMC Biotechnology

    Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

    doi: 10.1186/s12896-015-0139-7

    Figure Lengend Snippet: Sequencing of the fungal endophyte genome with the MspJI-based DNA fragmentation method. Two independent experiments (rep1 and rep2) were performed using the single endophyte strain. ( a ) WGA of the endophyte genome with the Φ29 enzyme in the presence of 5-methyl-dCTP (X μM). ( b ) MspJI digestion of the endophyte genome-derived amplicons. ( c ) Flowchart of the MspJI- and physical shearing-based library prep procedures from WGA products.

    Article Snippet: 5-methyl-dCTP (TriLink, CA, USA) was added to the PCR mixture at final concentrations of from 0 to 60 μM.

    Techniques: Sequencing, Whole Genome Amplification, Derivative Assay

    MspJI-enzymatic digestion of 5 m C-containing PCR and Φ29 products. ( a ) DNA fragments amplified with the locus-specific PCR primers for the Agro _gc50 sequence under the presence of 5-methyl-dCTP (0, 2, 4 or 8 μM). ( b ) MspJI-digested DNA fragments derived from PCR products with each locus-specific primers and Agro gDNA as DNA template. Molar concentration denotes the 5-methyl-dCTP-concentration in PCR solution. ( c ) MspJI-enzymatic digestion of Φ29 enzyme-amplified DNA with randomly incorporated 5 m C from a range of DNA templates. 0, 10, 15 and 20 μM denote final concentrations of 5-methyl-dCTP in the REPLI-g WGA mixture.

    Journal: BMC Biotechnology

    Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

    doi: 10.1186/s12896-015-0139-7

    Figure Lengend Snippet: MspJI-enzymatic digestion of 5 m C-containing PCR and Φ29 products. ( a ) DNA fragments amplified with the locus-specific PCR primers for the Agro _gc50 sequence under the presence of 5-methyl-dCTP (0, 2, 4 or 8 μM). ( b ) MspJI-digested DNA fragments derived from PCR products with each locus-specific primers and Agro gDNA as DNA template. Molar concentration denotes the 5-methyl-dCTP-concentration in PCR solution. ( c ) MspJI-enzymatic digestion of Φ29 enzyme-amplified DNA with randomly incorporated 5 m C from a range of DNA templates. 0, 10, 15 and 20 μM denote final concentrations of 5-methyl-dCTP in the REPLI-g WGA mixture.

    Article Snippet: 5-methyl-dCTP (TriLink, CA, USA) was added to the PCR mixture at final concentrations of from 0 to 60 μM.

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing, Derivative Assay, Concentration Assay, Whole Genome Amplification