5 fluorouridine Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Millipore 5 fluorouridine
    Disruption of TIF-IA mediated pre-rRNA synthesis is responsible for AICAR-induced apoptosis in LKB1-null/depleted cells A. H460 and H157 cells were plated on glass cover slips and treated with 1 mM AICAR for 12 hrs. Cells were labeled with 2 mM <t>5-fluorouridine</t> before immunofluorescence analysis. B. H460 and H157 cells were seeded in 60 mm dishes and treated with 1 mM AICAR, 2 mM uridine alone, or their combination. RNA was extracted with Trizol 12 hrs after AICAR treatment. Pre-RNA level was assessed by real-time PCR amplifying pre-45S ribosomal RNA using 18S rRNA as control. C. H460 and H157 cells were seeded in 6-well plates and treated with TIF-IA siRNA or control siRNA. Cell lysates were collected 48 hrs after transfection for immunoblot analysis and total caspase-3 was checked. D. H460 cells were stably infected with retrovirus containing the indicated TIF-IA wild-type or mutant constructs. Cells were treated with 1 mM AICAR for 24 hrs. Cell lysates were analyzed by immunoblot using indicated antibodies, including the one against cleaved caspase-3.
    5 Fluorouridine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouridine/product/Millipore
    Average 96 stars, based on 250 article reviews
    Price from $9.99 to $1999.99
    5 fluorouridine - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    99
    Millipore 2 deoxy 5 fluorouridine
    Disruption of TIF-IA mediated pre-rRNA synthesis is responsible for AICAR-induced apoptosis in LKB1-null/depleted cells A. H460 and H157 cells were plated on glass cover slips and treated with 1 mM AICAR for 12 hrs. Cells were labeled with 2 mM <t>5-fluorouridine</t> before immunofluorescence analysis. B. H460 and H157 cells were seeded in 60 mm dishes and treated with 1 mM AICAR, 2 mM uridine alone, or their combination. RNA was extracted with Trizol 12 hrs after AICAR treatment. Pre-RNA level was assessed by real-time PCR amplifying pre-45S ribosomal RNA using 18S rRNA as control. C. H460 and H157 cells were seeded in 6-well plates and treated with TIF-IA siRNA or control siRNA. Cell lysates were collected 48 hrs after transfection for immunoblot analysis and total caspase-3 was checked. D. H460 cells were stably infected with retrovirus containing the indicated TIF-IA wild-type or mutant constructs. Cells were treated with 1 mM AICAR for 24 hrs. Cell lysates were analyzed by immunoblot using indicated antibodies, including the one against cleaved caspase-3.
    2 Deoxy 5 Fluorouridine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 deoxy 5 fluorouridine/product/Millipore
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    2 deoxy 5 fluorouridine - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    TCI Tokyo Chemical Industry 2 deoxy 5 fluorouridine
    Disruption of TIF-IA mediated pre-rRNA synthesis is responsible for AICAR-induced apoptosis in LKB1-null/depleted cells A. H460 and H157 cells were plated on glass cover slips and treated with 1 mM AICAR for 12 hrs. Cells were labeled with 2 mM <t>5-fluorouridine</t> before immunofluorescence analysis. B. H460 and H157 cells were seeded in 60 mm dishes and treated with 1 mM AICAR, 2 mM uridine alone, or their combination. RNA was extracted with Trizol 12 hrs after AICAR treatment. Pre-RNA level was assessed by real-time PCR amplifying pre-45S ribosomal RNA using 18S rRNA as control. C. H460 and H157 cells were seeded in 6-well plates and treated with TIF-IA siRNA or control siRNA. Cell lysates were collected 48 hrs after transfection for immunoblot analysis and total caspase-3 was checked. D. H460 cells were stably infected with retrovirus containing the indicated TIF-IA wild-type or mutant constructs. Cells were treated with 1 mM AICAR for 24 hrs. Cell lysates were analyzed by immunoblot using indicated antibodies, including the one against cleaved caspase-3.
    2 Deoxy 5 Fluorouridine, supplied by TCI Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 deoxy 5 fluorouridine/product/TCI Tokyo Chemical Industry
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 deoxy 5 fluorouridine - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    89
    Becton Dickinson 5 fluorouridine
    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU <t>(5‐fluorouridine,</t> positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p
    5 Fluorouridine, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouridine/product/Becton Dickinson
    Average 89 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    5 fluorouridine - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    91
    Thermo Fisher 5 fluorouridine
    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU <t>(5‐fluorouridine,</t> positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p
    5 Fluorouridine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouridine/product/Thermo Fisher
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    5 fluorouridine - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    92
    Moravek Biochemicals h 5 deoxy 5 fluorouridine
    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU <t>(5‐fluorouridine,</t> positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p
    H 5 Deoxy 5 Fluorouridine, supplied by Moravek Biochemicals, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h 5 deoxy 5 fluorouridine/product/Moravek Biochemicals
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    h 5 deoxy 5 fluorouridine - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Synquest 2 deoxy 5 fluorouridine fdu
    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU <t>(5‐fluorouridine,</t> positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p
    2 Deoxy 5 Fluorouridine Fdu, supplied by Synquest, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 deoxy 5 fluorouridine fdu/product/Synquest
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 deoxy 5 fluorouridine fdu - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Horizon Discovery synthetic 5 fluorouridine containing oligonucleotide
    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU <t>(5‐fluorouridine,</t> positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p
    Synthetic 5 Fluorouridine Containing Oligonucleotide, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synthetic 5 fluorouridine containing oligonucleotide/product/Horizon Discovery
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    synthetic 5 fluorouridine containing oligonucleotide - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    99
    Millipore fluoro 2 deoxyuridine
    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU <t>(5‐fluorouridine,</t> positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p
    Fluoro 2 Deoxyuridine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluoro 2 deoxyuridine/product/Millipore
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    fluoro 2 deoxyuridine - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Becton Dickinson 5 fluorouridine 5 o β d galactopyranoside hela cells
    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of 5-fluorouridine-5′- O -β- d <t>-galactopyranoside</t> (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of <t>HeLa</t> cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).
    5 Fluorouridine 5 O β D Galactopyranoside Hela Cells, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouridine 5 o β d galactopyranoside hela cells/product/Becton Dickinson
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    5 fluorouridine 5 o β d galactopyranoside hela cells - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    86
    Tokyo Chemical Industry 5 fluorouridine furd
    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of 5-fluorouridine-5′- O -β- d <t>-galactopyranoside</t> (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of <t>HeLa</t> cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).
    5 Fluorouridine Furd, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouridine furd/product/Tokyo Chemical Industry
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    5 fluorouridine furd - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    89
    Carbosynth 5 fluorouridine
    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of <t>5-fluorouridine-5′-</t> O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).
    5 Fluorouridine, supplied by Carbosynth, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouridine/product/Carbosynth
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    5 fluorouridine - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    85
    AK Scientific 5 deoxy 5 fluorouridine
    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of <t>5-fluorouridine-5′-</t> O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).
    5 Deoxy 5 Fluorouridine, supplied by AK Scientific, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 deoxy 5 fluorouridine/product/AK Scientific
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 deoxy 5 fluorouridine - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Millipore cell permeable 5 fluorouridine
    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of <t>5-fluorouridine-5′-</t> O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).
    Cell Permeable 5 Fluorouridine, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell permeable 5 fluorouridine/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell permeable 5 fluorouridine - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Disruption of TIF-IA mediated pre-rRNA synthesis is responsible for AICAR-induced apoptosis in LKB1-null/depleted cells A. H460 and H157 cells were plated on glass cover slips and treated with 1 mM AICAR for 12 hrs. Cells were labeled with 2 mM 5-fluorouridine before immunofluorescence analysis. B. H460 and H157 cells were seeded in 60 mm dishes and treated with 1 mM AICAR, 2 mM uridine alone, or their combination. RNA was extracted with Trizol 12 hrs after AICAR treatment. Pre-RNA level was assessed by real-time PCR amplifying pre-45S ribosomal RNA using 18S rRNA as control. C. H460 and H157 cells were seeded in 6-well plates and treated with TIF-IA siRNA or control siRNA. Cell lysates were collected 48 hrs after transfection for immunoblot analysis and total caspase-3 was checked. D. H460 cells were stably infected with retrovirus containing the indicated TIF-IA wild-type or mutant constructs. Cells were treated with 1 mM AICAR for 24 hrs. Cell lysates were analyzed by immunoblot using indicated antibodies, including the one against cleaved caspase-3.

    Journal: Oncotarget

    Article Title: LKB1 promotes cell survival by modulating TIF-IA-mediated pre-ribosomal RNA synthesis under uridine downregulated conditions

    doi: 10.18632/oncotarget.6224

    Figure Lengend Snippet: Disruption of TIF-IA mediated pre-rRNA synthesis is responsible for AICAR-induced apoptosis in LKB1-null/depleted cells A. H460 and H157 cells were plated on glass cover slips and treated with 1 mM AICAR for 12 hrs. Cells were labeled with 2 mM 5-fluorouridine before immunofluorescence analysis. B. H460 and H157 cells were seeded in 60 mm dishes and treated with 1 mM AICAR, 2 mM uridine alone, or their combination. RNA was extracted with Trizol 12 hrs after AICAR treatment. Pre-RNA level was assessed by real-time PCR amplifying pre-45S ribosomal RNA using 18S rRNA as control. C. H460 and H157 cells were seeded in 6-well plates and treated with TIF-IA siRNA or control siRNA. Cell lysates were collected 48 hrs after transfection for immunoblot analysis and total caspase-3 was checked. D. H460 cells were stably infected with retrovirus containing the indicated TIF-IA wild-type or mutant constructs. Cells were treated with 1 mM AICAR for 24 hrs. Cell lysates were analyzed by immunoblot using indicated antibodies, including the one against cleaved caspase-3.

    Article Snippet: Uridine, cytidine, adenosine, thymidine, deoxythymidine triphosphate (dTTP), uracil, phenformin and 5-fluorouridine were purchased from Sigma-Aldrich Co. LLC, (St Louis, MO).

    Techniques: IA, Labeling, Immunofluorescence, Real-time Polymerase Chain Reaction, Transfection, Stable Transfection, Infection, Mutagenesis, Construct

    LKB1 kinase activity is required for the nuclear accumulation of TIF-IA after AICAR treatment A. Pre-rRNA synthesis was evaluated in H157-LKB1-WT or H157-LKB1-K78M cells by quantitative real-time PCR. K78M: Kinase-dead mutant. B. Pre-rRNA synthesis was evaluated by 5-fluorouridine incorporation. C. Alteration in endogenous TIF-IA protein level after 1 or 2 mM AICAR treatment for 24 hrs was evaluated by immunoblot in isogenic cells. D. Time course analysis of TIF-IA cellular localization after 1 mM AICAR treatment in H157 isogenic cells. E. Nuclear localization of ectopically-expressed 636A and 636D TIF-IA mutant in isogenic H460 cells after 1 mM AICAR treatment for 24 hrs was evaluated by immunoblot analysis. C: Cytoplasmic fraction; N: Nuclear fraction.

    Journal: Oncotarget

    Article Title: LKB1 promotes cell survival by modulating TIF-IA-mediated pre-ribosomal RNA synthesis under uridine downregulated conditions

    doi: 10.18632/oncotarget.6224

    Figure Lengend Snippet: LKB1 kinase activity is required for the nuclear accumulation of TIF-IA after AICAR treatment A. Pre-rRNA synthesis was evaluated in H157-LKB1-WT or H157-LKB1-K78M cells by quantitative real-time PCR. K78M: Kinase-dead mutant. B. Pre-rRNA synthesis was evaluated by 5-fluorouridine incorporation. C. Alteration in endogenous TIF-IA protein level after 1 or 2 mM AICAR treatment for 24 hrs was evaluated by immunoblot in isogenic cells. D. Time course analysis of TIF-IA cellular localization after 1 mM AICAR treatment in H157 isogenic cells. E. Nuclear localization of ectopically-expressed 636A and 636D TIF-IA mutant in isogenic H460 cells after 1 mM AICAR treatment for 24 hrs was evaluated by immunoblot analysis. C: Cytoplasmic fraction; N: Nuclear fraction.

    Article Snippet: Uridine, cytidine, adenosine, thymidine, deoxythymidine triphosphate (dTTP), uracil, phenformin and 5-fluorouridine were purchased from Sigma-Aldrich Co. LLC, (St Louis, MO).

    Techniques: Activity Assay, IA, Real-time Polymerase Chain Reaction, Mutagenesis

    Effect of Pol I transcription blocks and transcription rate on SNORD44 and its sdRNA and long form. ( A, B ) HeLa cells were treated with Actinomycin D (Act D, 50 ng/ml) and incubated for 3 hours. A Cells were stained for NPM ( green ), FBL ( red ) and DNA ( blue ). Merged images are shown. Scale bar 10 µm. B Cells were subjected to subcellular fractionation, RNA was isolated and Northern hybridization was conducted using SNORD44 5′ probe. ( C and D ) HeLa cells were transfected with control or RPA194-targeting siRNA and incubated for 48 hours. C Cells were stained for RPA194 ( red ) and DNA ( blue ). Merged images are shown. Scale bar 10 µm. D Nuclear (Nu) and cytoplasmic (Cyto) fractions were prepared, and RNA was isolated. Northern hybridization was conducted using SNORD44 5′ probe. ( E–G ) HeLa cells were starved for 48 hours in serum-depleted medium. ( E and F ) Cells were incubated with 5-fluorouridine (FU) for 1 h, detected with 5-BrdU antibodies ( E ) and quantified ( F ). The decrease in FU incorporation over days 1–3 of starvation is shown. Scale bar 10 µm. G RNA was isolated and Northern hybridization was conducted using SNORD44 5′ probe. ( B, D, G ) Signal intensities for 120 nt and sdRNAs were quantified and normalized against the mature SNORD44.

    Journal: PLoS ONE

    Article Title: RNA-Seq of the Nucleolus Reveals Abundant SNORD44-Derived Small RNAs

    doi: 10.1371/journal.pone.0107519

    Figure Lengend Snippet: Effect of Pol I transcription blocks and transcription rate on SNORD44 and its sdRNA and long form. ( A, B ) HeLa cells were treated with Actinomycin D (Act D, 50 ng/ml) and incubated for 3 hours. A Cells were stained for NPM ( green ), FBL ( red ) and DNA ( blue ). Merged images are shown. Scale bar 10 µm. B Cells were subjected to subcellular fractionation, RNA was isolated and Northern hybridization was conducted using SNORD44 5′ probe. ( C and D ) HeLa cells were transfected with control or RPA194-targeting siRNA and incubated for 48 hours. C Cells were stained for RPA194 ( red ) and DNA ( blue ). Merged images are shown. Scale bar 10 µm. D Nuclear (Nu) and cytoplasmic (Cyto) fractions were prepared, and RNA was isolated. Northern hybridization was conducted using SNORD44 5′ probe. ( E–G ) HeLa cells were starved for 48 hours in serum-depleted medium. ( E and F ) Cells were incubated with 5-fluorouridine (FU) for 1 h, detected with 5-BrdU antibodies ( E ) and quantified ( F ). The decrease in FU incorporation over days 1–3 of starvation is shown. Scale bar 10 µm. G RNA was isolated and Northern hybridization was conducted using SNORD44 5′ probe. ( B, D, G ) Signal intensities for 120 nt and sdRNAs were quantified and normalized against the mature SNORD44.

    Article Snippet: Nascent rRNA Synthesis Cells were incubated with 1 mM 5-fluorouridine (FU) (Sigma-Aldrich) using hypotonic shift and fixed with ice-cold methanol and acetone according to ref. .

    Techniques: Activated Clotting Time Assay, Incubation, Staining, Fractionation, Isolation, Northern Blot, Hybridization, Transfection

    Mechanism of action of capecitabine and proposed synergistic interaction with valproic acid Capecitabine is an oral fluoropyrimidine absorbed unchanged through the gastrointestinal wall and converted to 5′-DFUR in the liver, by the sequential action of CE and CyD, and to 5-FU in tumor cells by TP. The active metabolite FdUMP by binding and inhibiting TS, causes rapid depletion of dTTP and thus thymine-less apoptosis. FdUTP and FUTP can be incorporated into DNA and RNA, respectively, contributing to 5-FU cytotoxicity. VPA synergizes with capecitabine preferentially by up-regulating TP, while down-regulation of TS expression is only partially involved. Abbreviation: CE, carboxylesterase; 5-DFCR, 5′-deoxy-5-fluorocytidine; CyD, cytidine deaminase; 5-DFUR, 5′-deoxy-5-fluorouridine; TP, thymidine phosphorylase; 5-FU, 5-fluorouracil; OPRT, orotate phosphoribosyl transferase; FUMP, 5-fluorouridine monophosphate; FUDP, 5-fluorouridinediphosphate; RR, ribonucleotide reductase; FUTP, 5-fluorouridine triphosphate; F-dUrd, 5-fluoro-2′-deoxyuridine; TK, thymidine kinase; F-dUDP, 5-Fluoro-2′-deoxyuridine diphosphate; F-dUMP, 5-fluoro-2′-deoxyuridine monophosphate; dUMP, 2′-deoxyuridine monophosphate; TS, thymidylate synthase; F-dUTP, 5-Fluoro-2′-deoxyuridine triphosphate; NDK, nucleoside diphosphate kinase; UMP-CMPK, urdine monophosphate-cytidine monophosphate kinase; dTTP, Deoxythymidine triphosphate.

    Journal: Oncotarget

    Article Title: Valproic acid potentiates the anticancer activity of capecitabine in vitro and in vivo in breast cancer models via induction of thymidine phosphorylase expression

    doi: 10.18632/oncotarget.6802

    Figure Lengend Snippet: Mechanism of action of capecitabine and proposed synergistic interaction with valproic acid Capecitabine is an oral fluoropyrimidine absorbed unchanged through the gastrointestinal wall and converted to 5′-DFUR in the liver, by the sequential action of CE and CyD, and to 5-FU in tumor cells by TP. The active metabolite FdUMP by binding and inhibiting TS, causes rapid depletion of dTTP and thus thymine-less apoptosis. FdUTP and FUTP can be incorporated into DNA and RNA, respectively, contributing to 5-FU cytotoxicity. VPA synergizes with capecitabine preferentially by up-regulating TP, while down-regulation of TS expression is only partially involved. Abbreviation: CE, carboxylesterase; 5-DFCR, 5′-deoxy-5-fluorocytidine; CyD, cytidine deaminase; 5-DFUR, 5′-deoxy-5-fluorouridine; TP, thymidine phosphorylase; 5-FU, 5-fluorouracil; OPRT, orotate phosphoribosyl transferase; FUMP, 5-fluorouridine monophosphate; FUDP, 5-fluorouridinediphosphate; RR, ribonucleotide reductase; FUTP, 5-fluorouridine triphosphate; F-dUrd, 5-fluoro-2′-deoxyuridine; TK, thymidine kinase; F-dUDP, 5-Fluoro-2′-deoxyuridine diphosphate; F-dUMP, 5-fluoro-2′-deoxyuridine monophosphate; dUMP, 2′-deoxyuridine monophosphate; TS, thymidylate synthase; F-dUTP, 5-Fluoro-2′-deoxyuridine triphosphate; NDK, nucleoside diphosphate kinase; UMP-CMPK, urdine monophosphate-cytidine monophosphate kinase; dTTP, Deoxythymidine triphosphate.

    Article Snippet: Reagents Valproic acid (VPA) was purchased from Enzo Life Sciences and 5′-deoxy-5-fluorouridine (5′-DFUR) was purchased from Sigma-Aldrich.

    Techniques: Binding Assay, Expressing

    SmgGDS-558 physically interacts with UBF, and this interaction promotes the nucleolar accumulation of SmgGDS-558. ( a ) HEK293T cells were transfected with cDNAs encoding the HA vector or HA-tagged SmgGDS, followed by immunoprecipitation using HA antibody and silver staining to detect co-precipitating proteins. Mass spectrometry identified UBF as one of the co-precipitating proteins (HC and LC; heavy and light chains, respectively, of antibodies used in the immunoprecipitation). ( b ) Lysates from HEK293T cells transfected with cDNAs encoding the HA vector or HA-tagged SmgGDS were immunoprecipitated using HA antibody, followed by immunoblotting using antibodies to UBF and HA ( n =3). ( c ) HEK293T cells were transfected with cDNAs encoding the HA vector or SmgGDS-558-NES mut -HA along with non-targeting (NT) siRNA or UBF siRNA. After 72 h, the cells were immunofluorescently stained with HA antibody, UBF antibody and 4,6-diamidino-2-phenylindole (DAPI), and examined by confocal fluorescence microscopy ( n =3). ( d ) HEK293T cells expressing SmgGDS-558-NES mut -HA were treated with or without the RNA Pol I inhibitor CX-5461 (1 μ m , 2 h), followed by FuRD (2 m m , 15 min), and immunofluorescently stained using antibodies to HA and BrdU ( n =3). Images were obtained by confocal microscopy. ( e ) NCI-H1703 cells were transfected with the indicated siRNAs to deplete SmgGDS, and 72 h later quantitative PCR was conducted to examine 47S pre-rRNA levels (normalized to cellular GAPDH). Control cells were treated with CX-5461 (1 μ m , 2 h) before collecting RNA. Error bars represent ±s.e.m. of three biological replicates, and statistical significance was determined by one-way analysis of variance and Holm-Sidak multiple comparisons test (* P

    Journal: Oncogene

    Article Title: SmgGDS is a transient nucleolar protein that protects cells from nucleolar stress and promotes the cell cycle by regulating DREAM complex gene expression

    doi: 10.1038/onc.2017.280

    Figure Lengend Snippet: SmgGDS-558 physically interacts with UBF, and this interaction promotes the nucleolar accumulation of SmgGDS-558. ( a ) HEK293T cells were transfected with cDNAs encoding the HA vector or HA-tagged SmgGDS, followed by immunoprecipitation using HA antibody and silver staining to detect co-precipitating proteins. Mass spectrometry identified UBF as one of the co-precipitating proteins (HC and LC; heavy and light chains, respectively, of antibodies used in the immunoprecipitation). ( b ) Lysates from HEK293T cells transfected with cDNAs encoding the HA vector or HA-tagged SmgGDS were immunoprecipitated using HA antibody, followed by immunoblotting using antibodies to UBF and HA ( n =3). ( c ) HEK293T cells were transfected with cDNAs encoding the HA vector or SmgGDS-558-NES mut -HA along with non-targeting (NT) siRNA or UBF siRNA. After 72 h, the cells were immunofluorescently stained with HA antibody, UBF antibody and 4,6-diamidino-2-phenylindole (DAPI), and examined by confocal fluorescence microscopy ( n =3). ( d ) HEK293T cells expressing SmgGDS-558-NES mut -HA were treated with or without the RNA Pol I inhibitor CX-5461 (1 μ m , 2 h), followed by FuRD (2 m m , 15 min), and immunofluorescently stained using antibodies to HA and BrdU ( n =3). Images were obtained by confocal microscopy. ( e ) NCI-H1703 cells were transfected with the indicated siRNAs to deplete SmgGDS, and 72 h later quantitative PCR was conducted to examine 47S pre-rRNA levels (normalized to cellular GAPDH). Control cells were treated with CX-5461 (1 μ m , 2 h) before collecting RNA. Error bars represent ±s.e.m. of three biological replicates, and statistical significance was determined by one-way analysis of variance and Holm-Sidak multiple comparisons test (* P

    Article Snippet: The cells were cultured as previously described, and treated in some experiments with the following compounds: leptomycin B (L2913, Sigma, St Louis, MO, USA); MG-132 (Z-Leu-Leu-Leu-al, Sigma, C2211); lactacystin (Sigma, L6785); cycloheximide (239764, Calbiochem, San Diego, CA, USA); CX-5461 (Calbiochem, 509265); and FuRD (Sigma, F5130).

    Techniques: Transfection, Plasmid Preparation, Immunoprecipitation, Silver Staining, Mass Spectrometry, Staining, Fluorescence, Microscopy, Expressing, Confocal Microscopy, Real-time Polymerase Chain Reaction

    Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU (5‐fluorouridine, positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p

    Journal: ChemistryOpen

    Article Title: Nucleolipids of Canonical Purine ß‐d‐Ribo‐Nucleosides: Synthesis and Cytostatic/Cytotoxic Activities Toward Human and Rat Glioblastoma Cells

    doi: 10.1002/open.201500197

    Figure Lengend Snippet: Viability/survival of human astrocytoma/oligodendroglioma GOS‐3 cells after 48 h of incubation with 5‐FU (5‐fluorouridine, positive control) or its derivative 8 c . Values are given [in % survival of control (incubation with medium alone=100 % survival] as mean±SEM; ** p

    Article Snippet: The cells were cultured in 90 % RPMI 1640 medium supplemented with 10 % fetal bovine serum (FBS), 100 U mL−1 penicillin, and 0.1 mg mL−1 streptomycin, and were maintained at 37 °C in a humidified atmosphere (5 % CO2 , 95 % air) as described earlier., Determination of viability/survival of 5‐fluorouridine and derivatives : 96‐well plates (BD Falcon, Becton Dickinson GmbH, Heidelberg, Germany) were seeded with 1.5×104 GOS‐3, 5×103 BT4Ca, or 3×104 THP‐1 cells.

    Techniques: Incubation, Positive Control

    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of 5-fluorouridine-5′- O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).

    Journal: Scientific Reports

    Article Title: Molecular logistics using cytocleavable polyrotaxanes for the reactivation of enzymes delivered in living cells

    doi: 10.1038/srep02252

    Figure Lengend Snippet: Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of 5-fluorouridine-5′- O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).

    Article Snippet: Enzyme-prodrug assay with 5-fluorouridine-5′-O -β-d -galactopyranoside HeLa cells were seeded on a 96-well plate (BD Falcon) at a density of 2.5 × 104 cells/cm2 and incubated overnight.

    Techniques: Activation Assay, Incubation

    Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of 5-fluorouridine-5′- O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).

    Journal: Scientific Reports

    Article Title: Molecular logistics using cytocleavable polyrotaxanes for the reactivation of enzymes delivered in living cells

    doi: 10.1038/srep02252

    Figure Lengend Snippet: Activation of anticancer prodrugs by the DMAE-SS-PRX/β-gal complexes. (A) Chemical structures of 5-fluorouridine-5′- O -β- d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR). (B) Relative viability of HeLa cells incubated for 24 h with 5-FUR-β-gal (open circles) and 5-FUR (closed circles). (C) Relative viability of HeLa cells treated with Xfect/β-gal, cleavable DMAE-SS-PRX/β-gal complexes (N/C 2), and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) at various β-gal concentrations for 24 h, followed by incubation with or without 5-FUR-β-gal (100 μM) for 24 h. Data are expressed as the means ± SD (n = 6).

    Article Snippet: 5-Fluorouridine-5′-O -β-d -galactopyranoside (5-FUR-β-gal) and 5-fluorouridine (5-FUR) were obtained from Carbosynth (Berkshire, UK) and TCI (Tokyo, Japan), respectively.

    Techniques: Activation Assay, Incubation