Journal: Molecular Plant Pathology

Article Title: Species' identification and microarray‐based comparative genome analysis of Streptomyces species isolated from potato scab lesions in Norway

doi: 10.1111/j.1364-3703.2011.00741.x

Figure Lengend Snippet: Microarray analysis‐based grouping of the 28 pathogenic Streptomyces strains isolated from potato scab lesions in this study, including S. scabies (type strain ATCC 49173) and S. turgidiscabies (strain St32) for comparison. Clustering was performed on the basis of the signals from 12 766 probes designed for 8848 genes of S. scabies obtained in two independent hybridizations. The signal intensities of the microarray data were preprocessed, the local background signal intensity was subtracted and the signals were subsequently normalized after transformation into the log2 domain. The microarray data were clustered hierarchically using the R package pvclust. Clade I consists of the S. turgidiscabies strain St32 and 14 strains (nos. 3, 5, 6, 7, 8, 19, 22, 36, 37, 39, 43, 44, 46 and 48) isolated in this study. The remaining 14 strains (nos. 9, 10, 14, 15, 17, 18, 21, 26, 31, 32, 33, 35, 40 and 50) form clade II. The type strain of S. scabies (ATCC 49173) is not placed in either clade. Euclidean distances and P values calculated from 1000 bootstraps were used.

Article Snippet: Ss, S. scabies strain ATCC 49173; St, S. turgidiscabies strain St32.

Techniques: Microarray, Isolation, Transformation Assay

Journal: Molecular Plant Pathology

Article Title: Species' identification and microarray‐based comparative genome analysis of Streptomyces species isolated from potato scab lesions in Norway

doi: 10.1111/j.1364-3703.2011.00741.x

Figure Lengend Snippet: Phylogenetic analysis of Streptomyces strains based on the neighbour‐joining analysis of the 16S rRNA gene sequences, including Norwegian strains of Streptomyces (accession numbers: HQ441807-HQ441834), the type strains of S. scabies (strain ATCC 49173; accession no. AB026199), S. turgidiscabies (strain ATCC 700248; AB026221), S. europaeiscabiei (strain CFBP 4497; AJ007423), S. acidiscabies (strain ATCC49003; AB026220), S. luridiscabiei (strain S63; NR_025155), S. niveiscabiei (strain S78; AF361786), S. puniciscabiei (strain S77; NR_025156), S. stelliscabiei (strain CFBP4521; NR_025294) and S. turgidiscabies (strain St32; EU828541) for comparison. Bootstrap values >90 are shown. The bar indicates a distance of 0.002 substitutions per site.

Article Snippet: Ss, S. scabies strain ATCC 49173; St, S. turgidiscabies strain St32.

Techniques:

Journal: Molecular Plant Pathology

Article Title: Species' identification and microarray‐based comparative genome analysis of Streptomyces species isolated from potato scab lesions in Norway

doi: 10.1111/j.1364-3703.2011.00741.x

Figure Lengend Snippet: Clustering of the Streptomyces strains based on signal intensities from two independent hybridizations of 17 probes designed for four genes (tomA, nec1, txtA and txtB) of the pathogenicity islands of S. turgidiscabies and used for microarray analysis. Higher signal intensities are indicated with darker colours. Ss, S. scabies strain ATCC 49173; St, S. turgidiscabies strain St32.

Article Snippet: Ss, S. scabies strain ATCC 49173; St, S. turgidiscabies strain St32.

Techniques: Microarray

Journal:

Article Title: Horizontal Transfer of the Plant Virulence Gene, nec1 , and Flanking Sequences among Genetically Distinct Streptomyces Strains in the Diastatochromogenes Cluster

doi: 10.1128/AEM.68.2.738-744.2002

Figure Lengend Snippet: Source, phenotypic and genetic characteristics of pathogenic Streptomyces strains and selected nonpathogens

Article Snippet: Pairwise DNA-DNA hybridization of S. scabiei ATCC 49173 T and strains 84.174, DNK-G10,and DNK-G01 were 37, 36, and 50%, respectively (Table ).

Techniques: Isolation